Prupe.3G299900_v2.0.a1

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0021221
Physical Location & Seq
Reverse (-)
26503582 .. 26504615
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G299900.1

Sequence Viewer

Length: 537 bp
ATGGAGAAAACAAAATCACCCCTTCATGGTCACACTAACGGAGGTCTCACCACTGTTCTTTCTATTGATGGAGGTGGAATAAGAGGGATTATTCCAGGAGTTATGCTTGCATTCCTAGAGTCTATGCTTCAGAAGATAGATGGTGATCATGTCAGGCTTGTAGATTATCTTGATTGGGTTGTAGGGATGAGCACAGGTGGCCTTATGGCGTCCATGCTTACTACCCCAAATAAAAATAATCATCCTTTATATGCTGCTAAAGATATCGTCCCCTTTTATCGCCAACACTGCCTTAAGATTTTCCCCCAACCCAGATACGTATACAGCTCTCACATCGGCAAAATCATATATTATTTGAAATGTTTAGCAGGGCCCAAATATAATGGGAAGTCTTTATGTAAGCTGTTGAAGGAAACACTAGGAGACAAACATTTGCAGGACATGTTGACTAACGTTGCAATCCCAACTACTGACATGTTGGCTGATCGGAAAAGATCATTCGGCTCCACTGGTGGATCTTCTTATGAGAGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

179

Amino Acids

19.62

Weight (kDa)

9.3

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 321
AclI AACGTT 1 cut(s) 453
AclWI GGATC 1 cut(s) 523
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 26
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 2 cut(s) 441, 474
AgsI TTSAA 2 cut(s) 358, 409
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 2 cut(s) 327, 403
AluI AGCT 2 cut(s) 327, 403
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 2 cut(s) 50, 417
AlwI GGATC 1 cut(s) 523
AoxI GGCC 2 cut(s) 199, 371
ApaI GGGCCC 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 254
AspS9I GGNCC 2 cut(s) 371, 372
AsuHPI GGTGA 3 cut(s) 9, 40, 155
BaeGI GKGCMC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 62, 134
BciT130I CCWGG 1 cut(s) 96
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 50, 417
BfaI CTAG 2 cut(s) 116, 419
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 96
BmgT120I GGNCC 2 cut(s) 371, 372
BmiI GGNNCC 2 cut(s) 373, 505
BmrFI CCNGG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 319
BsaBI GATNNNNATC 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 96
BseGI GGATG 2 cut(s) 192, 241
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 514
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 241
BshFI GGCC 2 cut(s) 201, 373
BsiHKAI GWGCWC 1 cut(s) 194
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 2 cut(s) 50, 417
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 110
BsnI GGCC 2 cut(s) 201, 373
Bso31I GGTCTC 1 cut(s) 50
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 194, 375
Bsp143I GATC 4 cut(s) 145, 484, 494, 515
BspANI GGCC 2 cut(s) 201, 373
BspLI GGNNCC 2 cut(s) 373, 505
BspPI GGATC 1 cut(s) 523
BspTI CTTAAG 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 50
BsrI ACTGG 1 cut(s) 514
BssMI GATC 4 cut(s) 145, 484, 494, 515
BssNAI GTATAC 1 cut(s) 322
BssNI GRCGYC 1 cut(s) 209
Bst1107I GTATAC 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 55
BstACI GRCGYC 1 cut(s) 209
BstAFI CTTAAG 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 108
BstF5I GGATG 2 cut(s) 192, 241
BstKTI GATC 4 cut(s) 148, 487, 497, 518
BstMAI GTCTC 2 cut(s) 50, 417
BstMBI GATC 4 cut(s) 145, 484, 494, 515
BstMWI GCNNNNNNNGC 2 cut(s) 198, 288
BstNI CCWGG 1 cut(s) 96
BstNSI RCATGY 2 cut(s) 445, 478
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 375
BstSNI TACGTA 1 cut(s) 319
BstV1I GCAGC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
BstZ17I GTATAC 1 cut(s) 322
BsuRI GGCC 2 cut(s) 201, 373
BtsCI GGATG 2 cut(s) 192, 241
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 3 cut(s) 51, 286, 507
Cac8I GCNNGC 1 cut(s) 108
Cfr13I GGNCC 2 cut(s) 371, 372
CseI GACGC 1 cut(s) 198
CviAII CATG 5 cut(s) 26, 149, 214, 442, 475
CviJI RGCY 7 cut(s) 157, 201, 327, 373, 403, 482, 504
CviKI_1 RGCY 7 cut(s) 157, 201, 327, 373, 403, 482, 504
DpnI GATC 4 cut(s) 147, 486, 496, 517
DpnII GATC 4 cut(s) 145, 484, 494, 515
Eco105I TACGTA 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 94
EcoRV GATATC 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 375
FaeI CATG 5 cut(s) 29, 152, 217, 445, 478
FaqI GGGAC 1 cut(s) 254
FatI CATG 5 cut(s) 25, 148, 213, 441, 474
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 2 cut(s) 199, 228
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 2 cut(s) 116, 419
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 201, 373
HgaI GACGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 5 cut(s) 29, 152, 217, 445, 478
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 1 cut(s) 119
HphI GGTGA 3 cut(s) 9, 40, 155
Hpy166II GTNNAC 2 cut(s) 322, 447
Hpy188I TCNGA 2 cut(s) 132, 489
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 2 cut(s) 322, 447
HpyAV CCTTC 2 cut(s) 32, 403
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 2 cut(s) 318, 453
HpyCH4V TGCA 3 cut(s) 110, 436, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 288
HpySE526I ACGT 2 cut(s) 318, 453
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 5 cut(s) 29, 152, 217, 445, 478
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 145, 484, 494, 515
LmnI GCTCC 1 cut(s) 509
LpnPI CCDG 8 cut(s) 81, 108, 139, 180, 325, 354, 422, 495
Lsp1109I GCAGC 1 cut(s) 241
MaeI CTAG 2 cut(s) 116, 419
MaeII ACGT 2 cut(s) 318, 453
MaeIII GTNAC 1 cut(s) 29
MalI GATC 4 cut(s) 147, 486, 496, 517
MboI GATC 4 cut(s) 145, 484, 494, 515
MboII GAAGA 2 cut(s) 145, 510
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 2 cut(s) 194, 375
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 3 cut(s) 35, 65, 77
MseI TTAA 1 cut(s) 294
MspCI CTTAAG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 110
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 198, 288
NdeII GATC 4 cut(s) 145, 484, 494, 515
NlaIII CATG 5 cut(s) 29, 152, 217, 445, 478
NlaIV GGNNCC 2 cut(s) 373, 505
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 2 cut(s) 445, 478
PciI ACATGT 2 cut(s) 441, 474
PctI GAATGC 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Ppu21I YACGTR 1 cut(s) 319
PscI ACATGT 2 cut(s) 441, 474
Psp1406I AACGTT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 2 cut(s) 373, 505
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 2 cut(s) 371, 372
PsuI RGATCY 1 cut(s) 515
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 4 cut(s) 145, 484, 494, 515
Sau96I GGNCC 2 cut(s) 371, 372
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 2 cut(s) 194, 375
SetI ASST 7 cut(s) 46, 76, 199, 321, 329, 405, 456
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SnaBI TACGTA 1 cut(s) 319
SspMI CTAG 2 cut(s) 116, 419
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 2 cut(s) 321, 456
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 3 cut(s) 58, 293, 514
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 14
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 58, 293, 514
Vha464I CTTAAG 1 cut(s) 293
XceI RCATGY 2 cut(s) 445, 478
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 116, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.