Prupe.8G044000_v2.0.a1

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0021221
Physical Location & Seq
Forward (+)
4540182 .. 4541699
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G044000.1

Sequence Viewer

Length: 600 bp
ATGGAGAGAACACAATCATCCCTTCATTCTCCCACTAACGGAAGTCTCATTACTATTCTTAGTATTGATGGAGGTGGAATAAGAGGGATTATCGCAGGAGTTATACTCAAGTTCCTAGAGTTTGAGCTTCAAAAGCTGGATGGTAATCATGTAAGACTTGCAGATAATTTTCATATGGTTGCAGGGACGAGCACAGGTGGCCTTGTGGCGGCCATGCATACTACCCCAAACAAAAATAATCATCTCTTGTATGCTGCTAAAGATATCGTCCCCTTTTATCTAGAACACTGTCCTAAAGTCTTTTCTGAAAGCTTTGCAAGTTGTGGGATTAACCTTGAGTTGTGGGCTTTAAATAGAGATCTTTCCCTTCTACAAACAGGAAACTTTAGTTTCCTATTTTATTCAAGGTTCACCTCAACCTTGACCTCCCCTGTTTCTTCAATATCAGGGTTTGCTGCATCCTATAACCAATCCTCTATAGACTCTATCAAAATCCAAGAACGAATCTGCAAACGTAAAATCTCTGGGATCAACAGGGTCAAGTGTGTCCTTCAAATTCTTGGGTCTTTGAAGCTTGATGGAGGGTTAGTTCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

200

Amino Acids

21.65

Weight (kDa)

8.95

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 209
AclWI GGATC 1 cut(s) 536
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 555
AfiI CCNNNNNNNGG 2 cut(s) 38, 208
AgsI TTSAA 5 cut(s) 131, 405, 441, 554, 571
AloI GAACNNNNNNTCC 1 cut(s) 573
AluBI AGCT 4 cut(s) 127, 136, 312, 574
AluI AGCT 4 cut(s) 127, 136, 312, 574
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 1 cut(s) 536
AoxI GGCC 2 cut(s) 199, 210
ApeKI GCWGC 2 cut(s) 254, 455
ApoI RAATTY 1 cut(s) 555
AsuHPI GGTGA 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 34, 66
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 241, 442
BccI CCATC 3 cut(s) 62, 134, 572
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 2 cut(s) 116, 281
BfmI CTRYAG 1 cut(s) 477
BglII AGATCT 1 cut(s) 358
BisI GCNGC 3 cut(s) 210, 255, 456
BlsI GCNGC 3 cut(s) 211, 256, 457
BmsI GCATC 1 cut(s) 467
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BpuEI CTTGAG 2 cut(s) 92, 356
BsaBI GATNNNNATC 1 cut(s) 144
BsaXI ACNNNNNCTCC 1 cut(s) 573
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 208
Bse8I GATNNNNATC 1 cut(s) 144
BseGI GGATG 3 cut(s) 17, 145, 458
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 2 cut(s) 38, 208
BseXI GCAGC 2 cut(s) 241, 442
BshFI GGCC 2 cut(s) 201, 212
BsiHKAI GWGCWC 1 cut(s) 194
BslFI GGGAC 2 cut(s) 199, 254
BslI CCNNNNNNNGG 2 cut(s) 38, 208
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 2 cut(s) 199, 254
BsnI GGCC 2 cut(s) 201, 212
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 2 cut(s) 358, 528
BspACI CCGC 1 cut(s) 209
BspANI GGCC 2 cut(s) 201, 212
BspPI GGATC 1 cut(s) 536
BssMI GATC 2 cut(s) 358, 528
Bst4CI ACNGT 1 cut(s) 290
BstDEI CTNAG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 17, 145, 458
BstKTI GATC 2 cut(s) 361, 531
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 2 cut(s) 358, 528
BstMWI GCNNNNNNNGC 2 cut(s) 133, 198
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 241, 442
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 201, 212
BtsCI GGATG 3 cut(s) 17, 145, 458
BtsIMutI CAGTG 1 cut(s) 286
CviAII CATG 2 cut(s) 149, 214
CviJI RGCY 7 cut(s) 127, 136, 201, 212, 312, 347, 574
CviKI_1 RGCY 7 cut(s) 127, 136, 201, 212, 312, 347, 574
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 360, 530
DpnII GATC 2 cut(s) 358, 528
DraI TTTAAA 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 210
Eco32I GATATC 1 cut(s) 265
EcoRV GATATC 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 219
FaeI CATG 2 cut(s) 152, 217
FaiI YATR 9 cut(s) 104, 150, 174, 176, 215, 219, 252, 465, 479
FaqI GGGAC 2 cut(s) 199, 254
FatI CATG 2 cut(s) 148, 213
FauNDI CATATG 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 210, 255, 456
FokI GGATG 3 cut(s) 4, 152, 445
Fsp4HI GCNGC 3 cut(s) 210, 255, 456
FspBI CTAG 2 cut(s) 116, 281
GluI GCNGC 3 cut(s) 210, 255, 456
HaeIII GGCC 2 cut(s) 201, 212
Hin1II CATG 2 cut(s) 152, 217
HindIII AAGCTT 2 cut(s) 310, 572
HinfI GANTC 2 cut(s) 482, 504
HphI GGTGA 1 cut(s) 403
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 1 cut(s) 411
HpyAV CCTTC 3 cut(s) 32, 377, 560
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 514
HpyCH4V TGCA 6 cut(s) 161, 182, 217, 317, 458, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 198
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 514
Hsp92II CATG 2 cut(s) 152, 217
Kzo9I GATC 2 cut(s) 358, 528
Lsp1109I GCAGC 2 cut(s) 241, 442
LweI GCATC 1 cut(s) 467
MaeI CTAG 2 cut(s) 116, 281
MaeII ACGT 1 cut(s) 514
MalI GATC 2 cut(s) 360, 530
MboI GATC 2 cut(s) 358, 528
MboII GAAGA 1 cut(s) 429
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 2 cut(s) 166, 555
MlyI GAGTC 1 cut(s) 476
MnlI CCTC 6 cut(s) 65, 77, 424, 436, 484, 575
Mph1103I ATGCAT 1 cut(s) 219
MseI TTAA 2 cut(s) 330, 350
MwoI GCNNNNNNNGC 2 cut(s) 133, 198
NdeI CATATG 1 cut(s) 174
NdeII GATC 2 cut(s) 358, 528
NlaIII CATG 2 cut(s) 152, 217
NsiI ATGCAT 1 cut(s) 219
PfeI GAWTC 1 cut(s) 504
PkrI GCNGC 3 cut(s) 211, 256, 457
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
PsuI RGATCY 1 cut(s) 358
SaqAI TTAA 2 cut(s) 330, 350
SatI GCNGC 3 cut(s) 210, 255, 456
Sau3AI GATC 2 cut(s) 358, 528
SchI GAGTC 1 cut(s) 476
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 1 cut(s) 467
SfcI CTRYAG 1 cut(s) 477
SmlI CTYRAG 2 cut(s) 107, 335
SmoI CTYRAG 2 cut(s) 107, 335
Sse9I AATT 2 cut(s) 166, 555
SsiI CCGC 1 cut(s) 209
SspMI CTAG 2 cut(s) 116, 281
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 517
TasI AATT 2 cut(s) 166, 555
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 2 cut(s) 330, 350
Tru9I TTAA 2 cut(s) 330, 350
TscAI CASTG 1 cut(s) 293
TseI GCWGC 2 cut(s) 254, 455
TspDTI ATGAA 2 cut(s) 14, 161
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 293
XapI RAATTY 1 cut(s) 555
XbaI TCTAGA 1 cut(s) 280
XspI CTAG 2 cut(s) 116, 281
Zsp2I ATGCAT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.