Prupe.5G208000_v2.0.a1

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0018710
Physical Location & Seq
Reverse (-)
16574962 .. 16577617
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G208000.1

Sequence Viewer

Length: 1317 bp
ATGATTTTCAACAAGTATATGTGCCTCGTTCTCTCGGCTGTTTCGTTTATACTTAAAATTCCTCATGCAGCTGCTTATGACCCATTGGATCCGAATGGGAACATTACATTGAAATGGGATGTCAAATCCTGGACTTCCGATGGTTATGTGGCAGTTGTGACGATGAACAACTTTCAAATGTACCGGCACATCATGAGCCCTGGCTGGACCTTAGGATGGGTATGGGCAAAGAAAGAGGTGATCTGGTCCATGGTTGGAGCACAAGCCGTTGACCAAGGAGACTGTACCAAGTTTAAAGGAAACATACCACATTGTTGTAAGAAAAATCCTACAGTTGTTGACTTGCTCCCTGGTGTCCCCTACAACCAACAGATTGCCAATTGCTGCAAAGCTGGTGTTGTGGCATCATGGGGTCAAGACCCTTCAGCTGCTGTGTCTTCCTTCCAGCTGAGTGTTGGGCGCTCTGGAACTTCCAACAAAACCGTCAGACTACCAAAGAATTTCACTTTGCTTGGTCCAGGACCAGGCTACACTTGCAGCCAAGCAAAGATTGTGCGATCTACAGTTTTCAACTCACCTGATGGTCGTCGAAAAACTCAAGCGCTGATGACTTGGAATGTGATATGTACTTACTCACAGTTGCTGGCGTCTCGCTACCCAACCTGTTGTGTCTCTATGTCATCATTTTACAACTCTACAATCACTCCTTGTCCAACTTGTGCCTGTGGTTGCCGAGACAAAAAGAAATGCATTGTGAGTGATTCAAAGTTGTCGAGTGTTGTGGGAATACACACACCAACAAAGGATAACACCCCGCTGCTTCAGTGCACACAACATATGTGCCCAATTCGGGTACATTGGCATGTGAAAACAAACTACAAGCAGTATTGGCGTGTGAAGATTACCATAACTAATTTCAACCATCGGTTGAATTACACGCAGTGGACGCTTGTGGTTGAACATCCAAATCTCAACCATATTACGGAAGTTTTTAGCTTTGTCTACAAGCCCCTGACCCCATATCAATCCAAGAATGACACTGGTTTGTTTTACGGCATAAAATTCTACAACGACCTCCTAAAAGAAGCTGGGCCAGAGGGAAATGTACAATCAGAGTTGATTCTTGAGAAGAATGCAAACACATTCACATTCAAGGAGGGTTGGGGTTTCCCTCGTAAGGTCTATTTCAATGGGGATGAATGCATGATGCCCCAACCTGATGAATTCCCAGGTCTACCAAATGCTGCCCATACAAATCTAATTACAGTACCAAAATTGGCTCTCTCTTGGCTTCTGATGTTTCTGGCTCTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

439

Amino Acids

49.23

Weight (kDa)

9.13

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018710)

Species Orthologous Gene IDs
prunus_persica Prupe.5G208000_v2.0.a1 Prupe.5G208100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0183431
rosa_roxburghii Rroxscaffold_3G00270460
rosa_rugosa Rorug06G0457900
rosa_samantha Rh7AG058900 Rh7CG060600 Rh7DG058700
rosa_wichuraiana Rw7G004850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1002, 1234
AciI CCGC 1 cut(s) 815
AclWI GGATC 2 cut(s) 83, 96
AcsI RAATTY 4 cut(s) 57, 499, 1061, 1223
AcuI CTGAAG 2 cut(s) 408, 806
AcyI GRCGYC 1 cut(s) 647
AdeI CACNNNGTG 1 cut(s) 942
AfaI GTAC 6 cut(s) 182, 286, 628, 855, 1107, 1269
AfeI AGCGCT 1 cut(s) 603
AfiI CCNNNNNNNGG 5 cut(s) 216, 524, 850, 982, 1177
AjnI CCWGG 6 cut(s) 128, 199, 349, 517, 523, 1228
AluBI AGCT 6 cut(s) 71, 392, 428, 448, 996, 1088
AluI AGCT 6 cut(s) 71, 392, 428, 448, 996, 1088
Alw21I GWGCWC 2 cut(s) 262, 830
Alw26I GTCTC 4 cut(s) 273, 654, 676, 729
Alw44I GTGCAC 1 cut(s) 826
AlwI GGATC 2 cut(s) 83, 96
AlwNI CAGNNNCTG 2 cut(s) 431, 643
Aor51HI AGCGCT 1 cut(s) 603
AoxI GGCC 1 cut(s) 1091
ApaLI GTGCAC 1 cut(s) 826
ApeKI GCWGC 7 cut(s) 68, 71, 384, 428, 537, 817, 1244
ApoI RAATTY 4 cut(s) 57, 499, 1061, 1223
AspLEI GCGC 2 cut(s) 462, 604
AspS9I GGNCC 5 cut(s) 207, 246, 515, 521, 1091
AsuHPI GGTGA 2 cut(s) 250, 567
AvaII GGWCC 4 cut(s) 207, 246, 515, 521
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 830, 845
BamHI GGATCC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 2 cut(s) 262, 830
BbvI GCAGC 7 cut(s) 58, 80, 371, 415, 549, 804, 1231
BccI CCATC 4 cut(s) 134, 210, 575, 930
BceAI ACGGC 2 cut(s) 251, 1069
BciT130I CCWGG 6 cut(s) 130, 201, 351, 519, 525, 1230
BcoDI GTCTC 4 cut(s) 273, 654, 676, 729
BfmI CTRYAG 2 cut(s) 330, 561
BfoI RGCGCY 2 cut(s) 463, 605
BisI GCNGC 7 cut(s) 69, 72, 385, 429, 538, 818, 1245
BlsI GCNGC 7 cut(s) 70, 73, 386, 430, 539, 819, 1246
Bme1390I CCNGG 6 cut(s) 130, 201, 351, 519, 525, 1230
Bme18I GGWCC 4 cut(s) 207, 246, 515, 521
BmgT120I GGNCC 5 cut(s) 207, 246, 515, 521, 1091
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 6 cut(s) 130, 201, 351, 519, 525, 1230
BmsI GCATC 2 cut(s) 413, 1197
BpiI GAAGAC 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 582, 1145
BsaHI GRCGYC 1 cut(s) 647
BsaJI CCNNGG 5 cut(s) 199, 249, 274, 349, 1228
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 524, 850, 982, 1177
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 1045
Bse21I CCTNAGG 1 cut(s) 211
BseBI CCWGG 6 cut(s) 130, 201, 351, 519, 525, 1230
BseDI CCNNGG 5 cut(s) 199, 249, 274, 349, 1228
BseGI GGATG 4 cut(s) 124, 221, 961, 1201
BseLI CCNNNNNNNGG 5 cut(s) 216, 524, 850, 982, 1177
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 1045
BseSI GKGCMC 2 cut(s) 830, 845
BseXI GCAGC 7 cut(s) 58, 80, 371, 415, 549, 804, 1231
BseYI CCCAGC 1 cut(s) 1088
BshFI GGCC 1 cut(s) 1093
BsiHKAI GWGCWC 2 cut(s) 262, 830
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 5 cut(s) 216, 524, 850, 982, 1177
BsmAI GTCTC 4 cut(s) 273, 654, 676, 729
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 2 cut(s) 1138, 1205
BsnI GGCC 1 cut(s) 1093
Bsp1286I GDGCHC 4 cut(s) 200, 262, 830, 845
Bsp1407I TGTACA 1 cut(s) 1105
Bsp143I GATC 3 cut(s) 88, 240, 557
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 1 cut(s) 815
BspANI GGCC 1 cut(s) 1093
BspCNI CTCAG 1 cut(s) 441
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 2 cut(s) 83, 96
BspQI GCTCTTC 1 cut(s) 1314
BsrFI RCCGGY 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 1105
BsrI ACTGG 1 cut(s) 1045
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 5 cut(s) 199, 249, 274, 349, 1228
BssMI GATC 3 cut(s) 88, 240, 557
BssNI GRCGYC 1 cut(s) 647
BssT1I CCWWGG 2 cut(s) 249, 274
Bst2UI CCWGG 6 cut(s) 130, 201, 351, 519, 525, 1230
Bst4CI ACNGT 6 cut(s) 284, 334, 484, 565, 639, 1267
Bst6I CTCTTC 1 cut(s) 1314
BstACI GRCGYC 1 cut(s) 647
BstAUI TGTACA 1 cut(s) 1105
BstC8I GCNNGC 1 cut(s) 645
BstDEI CTNAG 2 cut(s) 211, 449
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 4 cut(s) 124, 221, 961, 1201
BstH2I RGCGCY 2 cut(s) 463, 605
BstHHI GCGC 2 cut(s) 462, 604
BstKTI GATC 3 cut(s) 91, 243, 560
BstMAI GTCTC 4 cut(s) 273, 654, 676, 729
BstMBI GATC 3 cut(s) 88, 240, 557
BstMWI GCNNNNNNNGC 3 cut(s) 534, 889, 946
BstNI CCWGG 6 cut(s) 130, 201, 351, 519, 525, 1230
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 6 cut(s) 128, 199, 349, 517, 523, 1228
BstSFI CTRYAG 2 cut(s) 330, 561
BstSLI GKGCMC 2 cut(s) 830, 845
BstV1I GCAGC 7 cut(s) 58, 80, 371, 415, 549, 804, 1231
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 1093
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 4 cut(s) 124, 221, 961, 1201
BtsI GCAGTG 1 cut(s) 947
BtsIMutI CAGTG 4 cut(s) 830, 947, 1038, 1312
Cac8I GCNNGC 1 cut(s) 645
CaiI CAGNNNCTG 2 cut(s) 431, 643
CciI TCATGA 1 cut(s) 192
CfoI GCGC 2 cut(s) 462, 604
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 5 cut(s) 207, 246, 515, 521, 1091
CseI GACGC 2 cut(s) 636, 955
Csp6I GTAC 6 cut(s) 181, 285, 627, 854, 1106, 1268
CviAII CATG 6 cut(s) 65, 193, 250, 408, 863, 1204
CviQI GTAC 6 cut(s) 181, 285, 627, 854, 1106, 1268
DdeI CTNAG 2 cut(s) 211, 449
DpnI GATC 3 cut(s) 90, 242, 559
DpnII GATC 3 cut(s) 88, 240, 557
DraI TTTAAA 1 cut(s) 295
DraIII CACNNNGTG 1 cut(s) 942
Eam1104I CTCTTC 1 cut(s) 1314
EarI CTCTTC 1 cut(s) 1314
Eco130I CCWWGG 2 cut(s) 249, 274
Eco24I GRGCYC 1 cut(s) 200
Eco47I GGWCC 4 cut(s) 207, 246, 515, 521
Eco47III AGCGCT 1 cut(s) 603
Eco57I CTGAAG 2 cut(s) 408, 806
Eco81I CCTNAGG 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 1223
EcoRII CCWGG 6 cut(s) 128, 199, 349, 517, 523, 1228
EcoT14I CCWWGG 2 cut(s) 249, 274
EcoT22I ATGCAT 2 cut(s) 752, 1205
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 2 cut(s) 249, 274
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 6 cut(s) 68, 196, 253, 411, 866, 1207
FaqI GGGAC 1 cut(s) 341
FatI CATG 6 cut(s) 64, 192, 249, 407, 862, 1203
FauI CCCGC 1 cut(s) 822
FauNDI CATATG 1 cut(s) 837
FblI GTMKAC 2 cut(s) 1002, 1234
Fnu4HI GCNGC 7 cut(s) 69, 72, 385, 429, 538, 818, 1245
FokI GGATG 4 cut(s) 131, 228, 948, 1208
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 7 cut(s) 69, 72, 385, 429, 538, 818, 1245
GlaI GCGC 2 cut(s) 461, 603
GluI GCNGC 7 cut(s) 69, 72, 385, 429, 538, 818, 1245
GsaI CCCAGC 1 cut(s) 1092
HaeII RGCGCY 2 cut(s) 463, 605
HaeIII GGCC 1 cut(s) 1093
HapII CCGG 1 cut(s) 184
HgaI GACGC 2 cut(s) 636, 955
HhaI GCGC 2 cut(s) 462, 604
Hin1I GRCGYC 1 cut(s) 647
Hin1II CATG 6 cut(s) 68, 196, 253, 411, 866, 1207
Hin6I GCGC 2 cut(s) 460, 602
HinP1I GCGC 2 cut(s) 460, 602
HincII GTYRAC 2 cut(s) 271, 340
HindII GTYRAC 2 cut(s) 271, 340
HinfI GANTC 2 cut(s) 761, 1120
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 250, 567
Hpy166II GTNNAC 6 cut(s) 271, 340, 828, 945, 1003, 1235
Hpy188I TCNGA 5 cut(s) 93, 139, 488, 1114, 1296
Hpy188III TCNNGA 4 cut(s) 193, 416, 465, 1124
Hpy8I GTNNAC 6 cut(s) 271, 340, 828, 945, 1003, 1235
Hpy99I CGWCG 1 cut(s) 591
HpyAV CCTTC 2 cut(s) 432, 451
HpyCH4III ACNGT 6 cut(s) 284, 334, 484, 565, 639, 1267
HpyCH4V TGCA 7 cut(s) 68, 387, 537, 750, 828, 1136, 1203
HpyF10VI GCNNNNNNNGC 3 cut(s) 534, 889, 946
HpyF3I CTNAG 2 cut(s) 211, 449
Hsp92I GRCGYC 1 cut(s) 647
Hsp92II CATG 6 cut(s) 68, 196, 253, 411, 866, 1207
HspAI GCGC 2 cut(s) 460, 602
Kzo9I GATC 3 cut(s) 88, 240, 557
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 2 cut(s) 257, 351
Lsp1109I GCAGC 7 cut(s) 58, 80, 371, 415, 549, 804, 1231
LweI GCATC 2 cut(s) 413, 1197
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 90, 242, 559
MboI GATC 3 cut(s) 88, 240, 557
MboII GAAGA 4 cut(s) 429, 910, 1141, 1301
MfeI CAATTG 1 cut(s) 379
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 4 cut(s) 200, 262, 830, 845
MmeI TCCRAC 3 cut(s) 235, 498, 737
MnlI CCTC 7 cut(s) 35, 72, 229, 1085, 1090, 1150, 1182
Mph1103I ATGCAT 2 cut(s) 752, 1205
MseI TTAA 2 cut(s) 54, 294
MslI CAYNNNNRTG 2 cut(s) 112, 861
MspA1I CMGCKG 4 cut(s) 71, 428, 448, 817
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 6 cut(s) 130, 201, 351, 519, 525, 1230
MunI CAATTG 1 cut(s) 379
Mva1269I GAATGC 2 cut(s) 1138, 1205
MvaI CCWGG 6 cut(s) 130, 201, 351, 519, 525, 1230
MwoI GCNNNNNNNGC 3 cut(s) 534, 889, 946
NcoI CCATGG 1 cut(s) 249
NdeI CATATG 1 cut(s) 837
NdeII GATC 3 cut(s) 88, 240, 557
NlaIII CATG 6 cut(s) 68, 196, 253, 411, 866, 1207
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 2 cut(s) 14, 758
NmuCI GTSAC 1 cut(s) 157
NsiI ATGCAT 2 cut(s) 752, 1205
NspI RCATGY 1 cut(s) 866
PagI TCATGA 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PctI GAATGC 2 cut(s) 1138, 1205
PfeI GAWTC 2 cut(s) 761, 1120
PfoI TCCNGGA 2 cut(s) 128, 517
PkrI GCNGC 7 cut(s) 70, 73, 386, 430, 539, 819, 1246
Psp6I CCWGG 6 cut(s) 128, 199, 349, 517, 523, 1228
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 6 cut(s) 128, 199, 349, 517, 523, 1228
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 5 cut(s) 207, 246, 515, 521, 1091
PstNI CAGNNNCTG 2 cut(s) 431, 643
PsuI RGATCY 1 cut(s) 88
PvuII CAGCTG 3 cut(s) 71, 428, 448
RsaI GTAC 6 cut(s) 182, 286, 628, 855, 1107, 1269
RsaNI GTAC 6 cut(s) 181, 285, 627, 854, 1106, 1268
RseI CAYNNNNRTG 2 cut(s) 112, 861
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 2 cut(s) 54, 294
SatI GCNGC 7 cut(s) 69, 72, 385, 429, 538, 818, 1245
Sau3AI GATC 3 cut(s) 88, 240, 557
Sau96I GGNCC 5 cut(s) 207, 246, 515, 521, 1091
ScrFI CCNGG 6 cut(s) 130, 201, 351, 519, 525, 1230
SduI GDGCHC 4 cut(s) 200, 262, 830, 845
SfaNI GCATC 2 cut(s) 413, 1197
SfcI CTRYAG 2 cut(s) 330, 561
SinI GGWCC 4 cut(s) 207, 246, 515, 521
SmiMI CAYNNNNRTG 2 cut(s) 112, 861
SmlI CTYRAG 2 cut(s) 597, 1124
SmoI CTYRAG 2 cut(s) 597, 1124
SsiI CCGC 1 cut(s) 815
StyD4I CCNGG 6 cut(s) 128, 199, 349, 517, 523, 1228
StyI CCWWGG 2 cut(s) 249, 274
TaaI ACNGT 6 cut(s) 284, 334, 484, 565, 639, 1267
TaqI TCGA 2 cut(s) 589, 773
TatI WGTACW 2 cut(s) 626, 1105
TfiI GAWTC 2 cut(s) 761, 1120
Tru1I TTAA 2 cut(s) 54, 294
Tru9I TTAA 2 cut(s) 54, 294
TscAI CASTG 3 cut(s) 830, 947, 1045
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 7 cut(s) 68, 71, 384, 428, 537, 817, 1244
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 3 cut(s) 179, 1212, 1236
TspGWI ACGGA 1 cut(s) 998
TspRI CASTG 3 cut(s) 830, 947, 1045
VneI GTGCAC 1 cut(s) 826
VpaK11BI GGWCC 4 cut(s) 207, 246, 515, 521
XapI RAATTY 4 cut(s) 57, 499, 1061, 1223
XceI RCATGY 1 cut(s) 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XmiI GTMKAC 2 cut(s) 1002, 1234
Zsp2I ATGCAT 2 cut(s) 752, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.