Rw7G004850

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0018710
Physical Location & Seq
Reverse (-)
4030012 .. 4032062
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004850.1

Sequence Viewer

Length: 1290 bp
ATGGCTTTGGCAATATTTTCTTCTGCAGTGGCTTATGATCCGTTTGATCCTTCTGGAAGCATAAATATCAAGTGGGATGTTTTGTCTTGGACCCCCGATGGCTATGTGGCAGCAGTGTCAATAAGCAACAACCAAATGTACCGGCAAATCACAAGCCCGGGATGGAGCATAGGATGGACATGGGCAAAGAAGGAAGTGATCTGGTCCATGGTGGGAGCTAAAGTGACCGATCAAGGGGACTGTTCGAAATTCAAAGGGAACACCCCACTTTGTTGTGAGAAAACTCCAACAGTTATTGACTTGCTTCCTGGAGCTCCTTACAACCAACAGTTCACCAATTGCTGCAAGGGTGGTGTTTTGACATCATTGGGACAAGACCCTTCTACTGCAGTCGCAGCATTTCAGATCAGTGTTGGCTTTGCAGGCACTTCAAATAAAACAGTAAAGCCTCCAAAGAAATTCTTTTTGCTTGGTCCTGGACCAGGCTATACTTGTAGTGCCGTGAACAGTGTGCCATCCTCGGTTTCCTTGTCACCTGATGGCCGAAGTAAAACTCGAGCAATGATGACATGGAGTTTGACTTGCACTTACTCACAATTGCTGGCCTCTAAAAGCCCAAAGTGCTGTGTGTCTCTGTCTTCCTTCTACAACCCCTTGATCACTCCTTGTCCATCTTGTGTTTGTGGTTGTAACGATGCAAATAATTGTGTGAATGACCTCAAGGATTCAAGTCTGCTACAATCAAAAACATCAGCCAAGGAAAATGCACCACAATTGTTACAATGCACAGAACACAAGTGCCCAATCCGAGTTCATTGGCATGTGAAAGCTAACTACAGAGAGTATTGGCGTGTGAAAATTACCATCACCAATTTTAACTTGCTCATGAATTACACACAATGGACACTAGTTGTTCAGCACCCAAATCTCAACAAACTGGTCAATATATCTAGCTTTCTCTATAAGCCACTTATCCAATACAGCCCCATAAATGACACTGGCATGTTTTATGGCATAAAATCTCTTAATGAACTAATAAGGGAAGCTGGGCCTAAGGGATATATCTACACAGAGTTGATTCTTAAGAAGGACAAGGATACATTCACATTAGATCAAGGTTGGGCATTTCCTTTGAGGCTTTACTTCAATGGTGATGAGTGCGTGATGCCCCTTCCTGATCTATACCCTTATCTACCCAACTCTGCTTTCTATAATTCATATCCAGTTTCAATATCATCTTCTACAGTACTGGCTACCTTTTTGGTTTTGGTACTGCTAGTCTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

429

Amino Acids

47.48

Weight (kDa)

8.7

Isoelectric Point (pI)

32.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 35 - 198 9.9e-62 COBRA-like protein
COB_C PF25079 207 - 396 1.1e-46 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018710)

Species Orthologous Gene IDs
prunus_persica Prupe.5G208000_v2.0.a1 Prupe.5G208100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0183431
rosa_roxburghii Rroxscaffold_3G00270460
rosa_rugosa Rorug06G0457900
rosa_samantha Rh7AG058900 Rh7CG060600 Rh7DG058700
rosa_wichuraiana Rw7G004850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 32, 41
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 2 cut(s) 248, 458
AfaI GTAC 3 cut(s) 140, 1248, 1272
AfiI CCNNNNNNNGG 2 cut(s) 234, 482
AflII CTTAAG 1 cut(s) 1082
AgsI TTSAA 5 cut(s) 253, 432, 729, 1147, 1230
AhlI ACTAGT 1 cut(s) 907
AjnI CCWGG 3 cut(s) 307, 475, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AluBI AGCT 5 cut(s) 218, 314, 830, 954, 1046
AluI AGCT 5 cut(s) 218, 314, 830, 954, 1046
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 636
AlwI GGATC 2 cut(s) 32, 41
Ama87I CYCGRG 2 cut(s) 157, 555
AoxI GGCC 3 cut(s) 541, 603, 1049
ApeKI GCWGC 3 cut(s) 110, 342, 395
ApoI RAATTY 2 cut(s) 248, 458
ArsI GACNNNNNNTTYG 2 cut(s) 559, 591
AspS9I GGNCC 5 cut(s) 90, 204, 473, 479, 1049
AsuC2I CCSGG 2 cut(s) 158, 159
AsuHPI GGTGA 4 cut(s) 325, 525, 859, 1163
AsuII TTCGAA 1 cut(s) 245
AvaI CYCGRG 2 cut(s) 157, 555
AvaII GGWCC 4 cut(s) 90, 204, 473, 479
AxyI CCTNAGG 1 cut(s) 1053
BaeGI GKGCMC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 3 cut(s) 122, 329, 407
BccI CCATC 7 cut(s) 92, 156, 168, 523, 533, 679, 872
BceAI ACGGC 1 cut(s) 485
BciT130I CCWGG 3 cut(s) 309, 477, 483
BciVI GTATCC 1 cut(s) 1090
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 2 cut(s) 158, 159
BcoDI GTCTC 1 cut(s) 636
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 3 cut(s) 908, 951, 1277
BfmI CTRYAG 4 cut(s) 24, 387, 835, 1242
BfrI CTTAAG 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 1090
BisI GCNGC 3 cut(s) 111, 343, 396
BlsI GCNGC 3 cut(s) 112, 344, 397
BmcAI AGTACT 1 cut(s) 1248
Bme1390I CCNGG 5 cut(s) 158, 159, 309, 477, 483
Bme18I GGWCC 4 cut(s) 90, 204, 473, 479
BmeT110I CYCGRG 2 cut(s) 157, 555
BmgT120I GGNCC 5 cut(s) 90, 204, 473, 479, 1049
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 5 cut(s) 158, 159, 309, 477, 483
BmsI GCATC 2 cut(s) 685, 1155
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 330
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 158, 159
BsaJI CCNNGG 4 cut(s) 157, 207, 519, 756
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 482
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 4 cut(s) 942, 1003, 1223, 1254
Bse21I CCTNAGG 1 cut(s) 1053
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 3 cut(s) 309, 477, 483
BseDI CCNNGG 4 cut(s) 157, 207, 519, 756
BseGI GGATG 4 cut(s) 82, 167, 179, 515
BseLI CCNNNNNNNGG 2 cut(s) 234, 482
BseMI GCAATG 1 cut(s) 567
BseNI ACTGG 4 cut(s) 942, 1003, 1223, 1254
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 3 cut(s) 122, 329, 407
BseYI CCCAGC 1 cut(s) 1046
BshFI GGCC 3 cut(s) 543, 605, 1051
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 2 cut(s) 157, 555
BsiSI CCGG 2 cut(s) 142, 158
BslFI GGGAC 2 cut(s) 251, 384
BslI CCNNNNNNNGG 2 cut(s) 234, 482
BsmAI GTCTC 1 cut(s) 636
BsmFI GGGAC 2 cut(s) 251, 384
BsnI GGCC 3 cut(s) 543, 605, 1051
BsoBI CYCGRG 2 cut(s) 157, 555
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 316, 803
Bsp143I GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
Bsp19I CCATGG 1 cut(s) 207
BspANI GGCC 3 cut(s) 543, 605, 1051
BspHI TCATGA 1 cut(s) 885
BspLI GGNNCC 1 cut(s) 92
BspMAI CTGCAG 2 cut(s) 28, 391
BspPI GGATC 2 cut(s) 32, 41
BspT104I TTCGAA 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 1082
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 4 cut(s) 942, 1003, 1223, 1254
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 4 cut(s) 157, 207, 519, 756
BssMI GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
BssT1I CCWWGG 2 cut(s) 207, 756
Bst2UI CCWGG 3 cut(s) 309, 477, 483
Bst4CI ACNGT 6 cut(s) 242, 292, 330, 442, 509, 1246
BstAFI CTTAAG 1 cut(s) 1082
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 424, 603
BstDEI CTNAG 1 cut(s) 1053
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 1 cut(s) 480
BstF5I GGATG 4 cut(s) 82, 167, 179, 515
BstKTI GATC 8 cut(s) 40, 49, 201, 232, 408, 660, 1114, 1180
BstMAI GTCTC 1 cut(s) 636
BstMBI GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
BstMWI GCNNNNNNNGC 3 cut(s) 395, 423, 621
BstNI CCWGG 3 cut(s) 309, 477, 483
BstNSI RCATGY 2 cut(s) 824, 1006
BstSCI CCNGG 5 cut(s) 156, 157, 307, 475, 481
BstSFI CTRYAG 4 cut(s) 24, 387, 835, 1242
BstSLI GKGCMC 1 cut(s) 803
BstV1I GCAGC 3 cut(s) 122, 329, 407
BstV2I GAAGAC 1 cut(s) 630
Bsu36I CCTNAGG 1 cut(s) 1053
BsuI GTATCC 1 cut(s) 1090
BsuRI GGCC 3 cut(s) 543, 605, 1051
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 4 cut(s) 82, 167, 179, 515
BtsI GCAGTG 2 cut(s) 33, 120
BtsIMutI CAGTG 5 cut(s) 33, 120, 415, 514, 996
Cac8I GCNNGC 2 cut(s) 424, 603
CciI TCATGA 1 cut(s) 885
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 5 cut(s) 90, 204, 473, 479, 1049
Cfr9I CCCGGG 1 cut(s) 157
Csp6I GTAC 3 cut(s) 139, 1247, 1271
CviAII CATG 6 cut(s) 180, 208, 570, 821, 886, 1003
CviQI GTAC 3 cut(s) 139, 1247, 1271
DdeI CTNAG 1 cut(s) 1053
DpnI GATC 8 cut(s) 39, 48, 200, 231, 407, 659, 1113, 1179
DpnII GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
EaeI YGGCCR 1 cut(s) 541
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 2 cut(s) 207, 756
Eco24I GRGCYC 1 cut(s) 316
Eco47I GGWCC 4 cut(s) 90, 204, 473, 479
Eco53kI GAGCTC 1 cut(s) 314
Eco81I CCTNAGG 1 cut(s) 1053
Eco88I CYCGRG 2 cut(s) 157, 555
EcoICRI GAGCTC 1 cut(s) 314
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoRII CCWGG 3 cut(s) 307, 475, 481
EcoT14I CCWWGG 2 cut(s) 207, 756
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 2 cut(s) 207, 756
FaeI CATG 6 cut(s) 183, 211, 573, 824, 889, 1006
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 2 cut(s) 251, 384
FatI CATG 6 cut(s) 179, 207, 569, 820, 885, 1002
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 111, 343, 396
FokI GGATG 4 cut(s) 89, 174, 186, 502
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 3 cut(s) 111, 343, 396
FspBI CTAG 3 cut(s) 908, 951, 1277
GluI GCNGC 3 cut(s) 111, 343, 396
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 3 cut(s) 543, 605, 1051
HapII CCGG 2 cut(s) 142, 158
Hin1II CATG 6 cut(s) 183, 211, 573, 824, 889, 1006
HinfI GANTC 2 cut(s) 725, 1078
HpaII CCGG 2 cut(s) 142, 158
HphI GGTGA 4 cut(s) 325, 525, 859, 1163
Hpy166II GTNNAC 2 cut(s) 333, 505
Hpy188I TCNGA 2 cut(s) 405, 809
Hpy188III TCNNGA 3 cut(s) 54, 886, 1175
Hpy8I GTNNAC 2 cut(s) 333, 505
HpyAV CCTTC 6 cut(s) 60, 184, 390, 652, 1081, 1181
HpyCH4III ACNGT 6 cut(s) 242, 292, 330, 442, 509, 1246
HpyCH4V TGCA 8 cut(s) 26, 345, 389, 422, 585, 698, 767, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 423, 621
HpyF3I CTNAG 1 cut(s) 1053
Hsp92II CATG 6 cut(s) 183, 211, 573, 824, 889, 1006
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
LmnI GCTCC 4 cut(s) 165, 215, 311, 319
Lsp1109I GCAGC 3 cut(s) 122, 329, 407
LweI GCATC 2 cut(s) 685, 1155
MaeI CTAG 3 cut(s) 908, 951, 1277
MaeIII GTNAC 4 cut(s) 223, 531, 689, 777
MalI GATC 8 cut(s) 39, 48, 200, 231, 407, 659, 1113, 1179
MboI GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
MboII GAAGA 3 cut(s) 12, 630, 1230
MfeI CAATTG 3 cut(s) 337, 596, 773
MhlI GDGCHC 2 cut(s) 316, 803
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 5 cut(s) 459, 529, 616, 728, 1128
MseI TTAA 3 cut(s) 876, 1026, 1083
MslI CAYNNNNRTG 2 cut(s) 819, 1001
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 2 cut(s) 142, 158
MspR9I CCNGG 5 cut(s) 158, 159, 309, 477, 483
MunI CAATTG 3 cut(s) 337, 596, 773
MvaI CCWGG 3 cut(s) 309, 477, 483
MwoI GCNNNNNNNGC 3 cut(s) 395, 423, 621
NciI CCSGG 2 cut(s) 158, 159
NcoI CCATGG 1 cut(s) 207
NdeII GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
NlaIII CATG 6 cut(s) 183, 211, 573, 824, 889, 1006
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 2 cut(s) 223, 531
NspI RCATGY 2 cut(s) 824, 1006
NspV TTCGAA 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 555
PagI TCATGA 1 cut(s) 885
PfeI GAWTC 2 cut(s) 725, 1078
PfoI TCCNGGA 2 cut(s) 307, 475
PkrI GCNGC 3 cut(s) 112, 344, 397
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 3 cut(s) 307, 475, 481
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 3 cut(s) 307, 475, 481
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 5 cut(s) 90, 204, 473, 479, 1049
PspXI VCTCGAGB 1 cut(s) 555
PstI CTGCAG 2 cut(s) 28, 391
RsaI GTAC 3 cut(s) 140, 1248, 1272
RsaNI GTAC 3 cut(s) 139, 1247, 1271
RseI CAYNNNNRTG 2 cut(s) 819, 1001
SacI GAGCTC 1 cut(s) 316
SaqAI TTAA 3 cut(s) 876, 1026, 1083
SatI GCNGC 3 cut(s) 111, 343, 396
Sau3AI GATC 8 cut(s) 37, 46, 198, 229, 405, 657, 1111, 1177
Sau96I GGNCC 5 cut(s) 90, 204, 473, 479, 1049
ScaI AGTACT 1 cut(s) 1248
ScrFI CCNGG 5 cut(s) 158, 159, 309, 477, 483
SduI GDGCHC 2 cut(s) 316, 803
SetI ASST 9 cut(s) 220, 316, 538, 720, 832, 956, 1048, 1120, 1259
SfaNI GCATC 2 cut(s) 685, 1155
SfcI CTRYAG 4 cut(s) 24, 387, 835, 1242
Sfr274I CTCGAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 245
SinI GGWCC 4 cut(s) 90, 204, 473, 479
SlaI CTCGAG 1 cut(s) 555
SmaI CCCGGG 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 819, 1001
SmlI CTYRAG 3 cut(s) 555, 719, 1082
SmoI CTYRAG 3 cut(s) 555, 719, 1082
SpeI ACTAGT 1 cut(s) 907
SspI AATATT 1 cut(s) 15
SspMI CTAG 3 cut(s) 908, 951, 1277
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 5 cut(s) 156, 157, 307, 475, 481
StyI CCWWGG 2 cut(s) 207, 756
TaaI ACNGT 6 cut(s) 242, 292, 330, 442, 509, 1246
TaqI TCGA 2 cut(s) 245, 556
TaqII GACCGA 1 cut(s) 242
TatI WGTACW 1 cut(s) 1246
TfiI GAWTC 2 cut(s) 725, 1078
Tru1I TTAA 3 cut(s) 876, 1026, 1083
Tru9I TTAA 3 cut(s) 876, 1026, 1083
TscAI CASTG 5 cut(s) 33, 120, 415, 514, 1003
TseFI GTSAC 2 cut(s) 223, 531
TseI GCWGC 3 cut(s) 110, 342, 395
Tsp45I GTSAC 2 cut(s) 223, 531
TspDTI ATGAA 4 cut(s) 803, 902, 1044, 1206
TspGWI ACGGA 1 cut(s) 30
TspMI CCCGGG 1 cut(s) 157
TspRI CASTG 5 cut(s) 33, 120, 415, 514, 1003
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 4 cut(s) 90, 204, 473, 479
XagI CCTNNNNNAGG 1 cut(s) 480
XapI RAATTY 2 cut(s) 248, 458
XceI RCATGY 2 cut(s) 824, 1006
XhoI CTCGAG 1 cut(s) 555
XmaI CCCGGG 1 cut(s) 157
XspI CTAG 3 cut(s) 908, 951, 1277
ZrmI AGTACT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.