Prupe.6G087100_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011729
Physical Location & Seq
Forward (+)
5889945 .. 5893597
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G087100.1

Sequence Viewer

Length: 1644 bp
ATGGAGGCAGAGGCACCAGCTGAGCAGCCGCAGGCAGCGCCGCCAAGCTTGCCACGGAAAAAGATGACGAAACAGTTAACGGGAAAGCGCGATGATACGCCTTTGCATTCTGCCGCAAGATCAGGAAATATGGGGGTGGTGATGGATATCCTTGAAAATACAGAAGAGGCTGAATTGAAGGAGTTATTGGCAAAGCAAAATTCATCTGATGAAACAGCCCTCTATGTTGCTGCAGAATATGGTTATGTTGATTTGGTCAGGGAGATTATCAAATACTATGATCTTGCTGATGCTGGAATCAAAGCAAGAAATGGGTTTGATGCATTCCACATTGCTGCCAAACAAGGGGATATGGATGTATTGAATGTGCTTATGGAGGCTCATCCAGAATTGACTATGACTGTGGATCTATCAAACACCACAGCTTTGCACACGGCTGCCACCCAAGGGCATACAGAGGTGGTGAACTTTCTATTGGAAGCAGGAAGCAGCCTGGCAACCATTGCTAGAAGCAATGGCAAAACCGCGCTGCATTCTGCAGCAAGAAATGGTCATTTGGTGGTTGTGAAAGCGCTTCTGGAAAAGGAGCCTGGTATTGCAACACGCGTCGATAAGAAGGGGCAGACGGCACTTCACATGGCAGCAAAGGGGCAGAATCTTGAGATGATGGAGGAGTTGATTAAGGCAGATCCTTCGTTGATAAACATGGCTGATAATAAAGACAACACGGCATTGCATATAGCTACTCGAAAGGGCAGAGCTGAGATTGTTAAGTTGCTACTTGAACACAGTGAAACCGACACAAAAGCTGTTAACAAGTCTGCAGAAACTGCCCTTGACACTGCCGAGAAAACTGGGAACTCGGACATTAAAGCTTTTCTCCAAGAGCGCGGTGTCCAGAGTGCTAAAGAAATCAAGCCACAGGCTACAAACCCAGCTCGAGAGCTGAAACAAACTGTGAGCGACATAAAGCACGAAGTCCACCACCAGCTAGAACACACTCGCCAAACCAGAAGACGCGTCCAGGGCATTGCGAAACGTCTTCACAAAATGCACACAGAAGGCCTTAACAATGCAATAAACTCCAACACTGTTGTTGCTGTCCTCATTGCCACGGTTACCTTTGCAGCCATTTACCAAATCCCTGGCCAATATGTGGATGACCCGAAAGACATTCCTCATGGGCAGTCGCTTGGGGAAGCCAACATTGCTCCCAAACTGGCCTTTATGATCTTCTTCATCTTTGATTCCATTGCCCTTTTCATTTCCCTGGCTGTTGTGGTGGTGCAAACTTCAGTGGTGGTGATTGAGAGCAAGGCCAAGAAGCAGATGATGGCAATCATTAACAAGCTCATGTGGTTGGCTTGTGTTCTGGTTTCTGTGGCATTTTTGGCACTCTCTTTTGTAGTGGTGGGTGGCCAGAAGTGGCTAGCAATTGGAGTTACAATTATAGGAACAGTCATCATGGCTGCGACTTTGGGCACTATGTGTTTCTGGGTAGTTAGGCATCGAATTGAGGCAAAAAACATGAGGAGCATTCGGAGATCATCGCTGGGCAGCAGATCGCGGTCGTTGTCGTTGTCTGCAGTGATTTCTGAATCTGAGCTCCTCAACAATGACTACAAGAAGATGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

548

Amino Acids

59.78

Weight (kDa)

8.53

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07710 AT1G07710 AT5G60070
fragaria_vesca FvH4_3g34650 FvH4_3g34650
malus_domestica MD03G1103100.v1.1 MD11G1116700.v1.1
prunus_persica Prupe.6G087100_v2.0.a1
pyrus_communis pycom03g08370 pycom11g09810
rosa_chinensis RchiOBHm_Chr5g0062351
rosa_laevigata RLG00000035552
rosa_multiflora Rmu_sc0010221.1_g000002
rosa_roxburghii Rroxscaffold_1G00018330
rosa_rugosa Rorug05G0348900
rosa_samantha Rh5BG422800 Rh5CG446800 Rh5DG437600
rosa_wichuraiana Rw5G038520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 1156
AccII CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
AciI CCGC 6 cut(s) 29, 41, 114, 525, 891, 1567
AclWI GGATC 2 cut(s) 414, 683
AcoI YGGCCR 2 cut(s) 1147, 1417
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 1278
AdeI CACNNNGTG 1 cut(s) 1488
AfeI AGCGCT 1 cut(s) 573
AfiI CCNNNNNNNGG 3 cut(s) 345, 447, 1156
AflIII ACRYGT 2 cut(s) 604, 1018
AgsI TTSAA 4 cut(s) 155, 178, 364, 785
AjnI CCWGG 5 cut(s) 492, 589, 1023, 1144, 1269
AjuI GAANNNNNNNTTGG 4 cut(s) 170, 202, 458, 490
Alw21I GWGCWC 1 cut(s) 1608
AlwI GGATC 2 cut(s) 414, 683
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 939
Aor51HI AGCGCT 1 cut(s) 573
AoxI GGCC 5 cut(s) 1063, 1147, 1221, 1317, 1417
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 5 cut(s) 40, 90, 529, 574, 891
AsuHPI GGTGA 3 cut(s) 151, 475, 1315
AsuNHI GCTAGC 1 cut(s) 1429
AvaI CYCGRG 1 cut(s) 939
BaeGI GKGCMC 1 cut(s) 1484
BalI TGGCCA 2 cut(s) 1149, 1419
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 1608
BbsI GAAGAC 2 cut(s) 1021, 1034
Bbv12I GWGCWC 1 cut(s) 1608
BccI CCATC 3 cut(s) 136, 661, 1327
BceAI ACGGC 3 cut(s) 450, 642, 744
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 5 cut(s) 494, 591, 1025, 1146, 1271
BfaI CTAG 3 cut(s) 507, 992, 1430
BfmI CTRYAG 4 cut(s) 231, 537, 822, 1584
BfoI RGCGCY 2 cut(s) 41, 575
BlpI GCTNAGC 1 cut(s) 21
Bme1390I CCNGG 5 cut(s) 494, 591, 1025, 1146, 1271
BmeT110I CYCGRG 1 cut(s) 939
BmiI GGNNCC 2 cut(s) 15, 588
BmrFI CCNGG 5 cut(s) 494, 591, 1025, 1146, 1271
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 280, 310, 1516
BmtI GCTAGC 1 cut(s) 1433
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 2 cut(s) 1021, 1034
Bpu1102I GCTNAGC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 680
BsaBI GATNNNNATC 2 cut(s) 146, 1337
BsaJI CCNNGG 6 cut(s) 53, 445, 1024, 1113, 1144, 1269
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 447, 1156
Bse1I ACTGG 2 cut(s) 859, 1224
Bse3DI GCAATG 8 cut(s) 330, 501, 520, 731, 1029, 1107, 1206, 1251
Bse8I GATNNNNATC 2 cut(s) 146, 1337
BseBI CCWGG 5 cut(s) 494, 591, 1025, 1146, 1271
BseDI CCNNGG 6 cut(s) 53, 445, 1024, 1113, 1144, 1269
BseGI GGATG 3 cut(s) 361, 382, 1165
BseJI GATNNNNATC 2 cut(s) 146, 1337
BseLI CCNNNNNNNGG 3 cut(s) 345, 447, 1156
BseMI GCAATG 8 cut(s) 330, 501, 520, 731, 1029, 1107, 1206, 1251
BseMII CTCAG 3 cut(s) 12, 753, 1593
BseNI ACTGG 2 cut(s) 859, 1224
BseRI GAGGAG 3 cut(s) 686, 1546, 1598
BseSI GKGCMC 1 cut(s) 1484
BseYI CCCAGC 2 cut(s) 934, 1552
Bsh1236I CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
Bsh1285I CGRYCG 1 cut(s) 1571
BshFI GGCC 5 cut(s) 1065, 1149, 1223, 1319, 1419
BshNI GGYRCC 1 cut(s) 13
BsiEI CGRYCG 1 cut(s) 1571
BsiHKAI GWGCWC 1 cut(s) 1608
BsiHKCI CYCGRG 1 cut(s) 939
BslI CCNNNNNNNGG 3 cut(s) 345, 447, 1156
BsmI GAATGC 4 cut(s) 106, 323, 532, 1536
BsnI GGCC 5 cut(s) 1065, 1149, 1223, 1319, 1419
BsoBI CYCGRG 1 cut(s) 939
Bsp1286I GDGCHC 2 cut(s) 1484, 1608
Bsp143I GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
Bsp1720I GCTNAGC 1 cut(s) 21
BspACI CCGC 6 cut(s) 29, 41, 114, 525, 891, 1567
BspANI GGCC 5 cut(s) 1065, 1149, 1223, 1319, 1419
BspCNI CTCAG 3 cut(s) 13, 754, 1594
BspFNI CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
BspLI GGNNCC 2 cut(s) 15, 588
BspMAI CTGCAG 4 cut(s) 235, 541, 826, 1588
BspOI GCTAGC 1 cut(s) 1433
BspPI GGATC 2 cut(s) 414, 683
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 8 cut(s) 330, 501, 520, 731, 1029, 1107, 1206, 1251
BsrI ACTGG 2 cut(s) 859, 1224
BssECI CCNNGG 6 cut(s) 53, 445, 1024, 1113, 1144, 1269
BssMI GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 5 cut(s) 494, 591, 1025, 1146, 1271
Bst4CI ACNGT 7 cut(s) 75, 403, 791, 958, 1093, 1117, 1459
Bst6I CTCTTC 1 cut(s) 159
BstAPI GCANNNNNTGC 2 cut(s) 503, 830
BstC8I GCNNGC 3 cut(s) 33, 50, 1431
BstDEI CTNAG 3 cut(s) 21, 762, 1602
BstDSI CCRYGG 2 cut(s) 53, 1113
BstEII GGTNACC 1 cut(s) 1117
BstF5I GGATG 3 cut(s) 361, 382, 1165
BstFNI CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
BstH2I RGCGCY 2 cut(s) 41, 575
BstHHI GCGC 5 cut(s) 40, 90, 529, 574, 891
BstKTI GATC 7 cut(s) 122, 283, 409, 691, 1233, 1547, 1565
BstMBI GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
BstMCI CGRYCG 1 cut(s) 1571
BstMWI GCNNNNNNNGC 7 cut(s) 37, 49, 503, 830, 1026, 1208, 1391
BstNI CCWGG 5 cut(s) 494, 591, 1025, 1146, 1271
BstPI GGTNACC 1 cut(s) 1117
BstSCI CCNGG 5 cut(s) 492, 589, 1023, 1144, 1269
BstSFI CTRYAG 4 cut(s) 231, 537, 822, 1584
BstSLI GKGCMC 1 cut(s) 1484
BstUI CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
BstV2I GAAGAC 2 cut(s) 1021, 1034
BstX2I RGATCY 2 cut(s) 406, 688
BstXI CCANNNNNNTGG 1 cut(s) 1145
BstYI RGATCY 2 cut(s) 406, 688
BsuRI GGCC 5 cut(s) 1065, 1149, 1223, 1319, 1419
BtgI CCRYGG 2 cut(s) 53, 1113
BtgZI GCGATG 2 cut(s) 105, 1533
BtsCI GGATG 3 cut(s) 361, 382, 1165
BtsI GCAGTG 2 cut(s) 840, 1593
BtsIMutI CAGTG 5 cut(s) 796, 840, 1089, 1302, 1593
Cac8I GCNNGC 3 cut(s) 33, 50, 1431
CaiI CAGNNNCTG 1 cut(s) 830
CfoI GCGC 5 cut(s) 40, 90, 529, 574, 891
CseI GACGC 3 cut(s) 595, 1009, 1026
CviAII CATG 6 cut(s) 637, 706, 1181, 1354, 1465, 1528
DdeI CTNAG 3 cut(s) 21, 762, 1602
DpnI GATC 7 cut(s) 121, 282, 408, 690, 1232, 1546, 1564
DpnII GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
DraIII CACNNNGTG 1 cut(s) 1488
EaeI YGGCCR 2 cut(s) 1147, 1417
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 1606
Eco130I CCWWGG 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 1608
Eco32I GATATC 1 cut(s) 148
Eco47III AGCGCT 1 cut(s) 573
Eco53kI GAGCTC 1 cut(s) 1606
Eco57I CTGAAG 1 cut(s) 1278
Eco88I CYCGRG 1 cut(s) 939
Eco91I GGTNACC 1 cut(s) 1117
EcoICRI GAGCTC 1 cut(s) 1606
EcoO65I GGTNACC 1 cut(s) 1117
EcoRII CCWGG 5 cut(s) 492, 589, 1023, 1144, 1269
EcoRV GATATC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 445
EcoT22I ATGCAT 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 1608
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 6 cut(s) 640, 709, 1184, 1357, 1468, 1531
FatI CATG 6 cut(s) 636, 705, 1180, 1353, 1464, 1527
FokI GGATG 3 cut(s) 368, 369, 1172
FriOI GRGCYC 1 cut(s) 1608
FspBI CTAG 3 cut(s) 507, 992, 1430
GlaI GCGC 5 cut(s) 39, 89, 528, 573, 890
GsaI CCCAGC 2 cut(s) 938, 1556
HaeII RGCGCY 2 cut(s) 41, 575
HaeIII GGCC 5 cut(s) 1065, 1149, 1223, 1319, 1419
HgaI GACGC 3 cut(s) 595, 1009, 1026
HhaI GCGC 5 cut(s) 40, 90, 529, 574, 891
Hin1II CATG 6 cut(s) 640, 709, 1184, 1357, 1468, 1531
Hin6I GCGC 5 cut(s) 38, 88, 527, 572, 889
HinP1I GCGC 5 cut(s) 38, 88, 527, 572, 889
HincII GTYRAC 2 cut(s) 78, 814
HindII GTYRAC 2 cut(s) 78, 814
HindIII AAGCTT 2 cut(s) 46, 873
HinfI GANTC 4 cut(s) 297, 655, 1247, 1598
HpaI GTTAAC 2 cut(s) 78, 814
HphI GGTGA 3 cut(s) 151, 475, 1315
Hpy166II GTNNAC 4 cut(s) 78, 466, 814, 982
Hpy188I TCNGA 5 cut(s) 208, 865, 1542, 1597, 1603
Hpy188III TCNNGA 6 cut(s) 123, 386, 578, 659, 898, 941
Hpy8I GTNNAC 4 cut(s) 78, 466, 814, 982
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 4 cut(s) 172, 610, 702, 1055
HpyCH4III ACNGT 7 cut(s) 75, 403, 791, 958, 1093, 1117, 1459
HpyCH4IV ACGT 1 cut(s) 1039
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 49, 503, 830, 1026, 1208, 1391
HpyF3I CTNAG 3 cut(s) 21, 762, 1602
HpySE526I ACGT 1 cut(s) 1039
Hsp92II CATG 6 cut(s) 640, 709, 1184, 1357, 1468, 1531
HspAI GCGC 5 cut(s) 38, 88, 527, 572, 889
KspAI GTTAAC 2 cut(s) 78, 814
Kzo9I GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
LmnI GCTCC 4 cut(s) 586, 1216, 1533, 1611
LweI GCATC 3 cut(s) 280, 310, 1516
MaeI CTAG 3 cut(s) 507, 992, 1430
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 2 cut(s) 1117, 1441
MalI GATC 7 cut(s) 121, 282, 408, 690, 1232, 1546, 1564
MboI GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
MboII GAAGA 6 cut(s) 176, 1026, 1034, 1225, 1228, 1639
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 2 cut(s) 406, 688
MhlI GDGCHC 2 cut(s) 1484, 1608
MlsI TGGCCA 2 cut(s) 1149, 1419
MluCI AATT 7 cut(s) 173, 199, 389, 1434, 1446, 1512, 1638
MluI ACGCGT 2 cut(s) 604, 1018
MluNI TGGCCA 2 cut(s) 1149, 1419
MmeI TCCRAC 1 cut(s) 1110
Mox20I TGGCCA 2 cut(s) 1149, 1419
Mph1103I ATGCAT 1 cut(s) 325
MscI TGGCCA 2 cut(s) 1149, 1419
MseI TTAA 7 cut(s) 77, 681, 771, 813, 870, 1068, 1344
Msp20I TGGCCA 2 cut(s) 1149, 1419
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 5 cut(s) 494, 591, 1025, 1146, 1271
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 4 cut(s) 106, 323, 532, 1536
MvaI CCWGG 5 cut(s) 494, 591, 1025, 1146, 1271
MvnI CGCG 6 cut(s) 90, 527, 606, 891, 1020, 1567
MwoI GCNNNNNNNGC 7 cut(s) 37, 49, 503, 830, 1026, 1208, 1391
NdeII GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
NheI GCTAGC 1 cut(s) 1429
NlaIII CATG 6 cut(s) 640, 709, 1184, 1357, 1468, 1531
NlaIV GGNNCC 2 cut(s) 15, 588
NmeAIII GCCGAG 1 cut(s) 871
NsiI ATGCAT 1 cut(s) 325
PaeR7I CTCGAG 1 cut(s) 939
PceI AGGCCT 1 cut(s) 1065
PctI GAATGC 4 cut(s) 106, 323, 532, 1536
PfeI GAWTC 4 cut(s) 297, 655, 1247, 1598
PflMI CCANNNNNTGG 1 cut(s) 1156
Psp124BI GAGCTC 1 cut(s) 1608
Psp6I CCWGG 5 cut(s) 492, 589, 1023, 1144, 1269
PspEI GGTNACC 1 cut(s) 1117
PspFI CCCAGC 2 cut(s) 934, 1552
PspGI CCWGG 5 cut(s) 492, 589, 1023, 1144, 1269
PspN4I GGNNCC 2 cut(s) 15, 588
PstI CTGCAG 4 cut(s) 235, 541, 826, 1588
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 406, 688
PvuII CAGCTG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 1608
SaqAI TTAA 7 cut(s) 77, 681, 771, 813, 870, 1068, 1344
Sau3AI GATC 7 cut(s) 119, 280, 406, 688, 1230, 1544, 1562
ScrFI CCNGG 5 cut(s) 494, 591, 1025, 1146, 1271
SduI GDGCHC 2 cut(s) 1484, 1608
SfaNI GCATC 3 cut(s) 280, 310, 1516
SfcI CTRYAG 4 cut(s) 231, 537, 822, 1584
Sfr274I CTCGAG 1 cut(s) 939
SlaI CTCGAG 1 cut(s) 939
SmlI CTYRAG 2 cut(s) 659, 939
SmoI CTYRAG 2 cut(s) 659, 939
Sse9I AATT 7 cut(s) 173, 199, 389, 1434, 1446, 1512, 1638
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 6 cut(s) 29, 41, 114, 525, 891, 1567
SspMI CTAG 3 cut(s) 507, 992, 1430
SstI GAGCTC 1 cut(s) 1608
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 5 cut(s) 492, 589, 1023, 1144, 1269
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 7 cut(s) 75, 403, 791, 958, 1093, 1117, 1459
TaiI ACGT 1 cut(s) 1042
TaqI TCGA 4 cut(s) 609, 748, 940, 1510
TasI AATT 7 cut(s) 173, 199, 389, 1434, 1446, 1512, 1638
TauI GCSGC 3 cut(s) 31, 43, 116
TfiI GAWTC 4 cut(s) 297, 655, 1247, 1598
Tru1I TTAA 7 cut(s) 77, 681, 771, 813, 870, 1068, 1344
Tru9I TTAA 7 cut(s) 77, 681, 771, 813, 870, 1068, 1344
TscAI CASTG 5 cut(s) 796, 847, 1096, 1302, 1593
TspDTI ATGAA 4 cut(s) 192, 225, 1228, 1252
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 5 cut(s) 796, 847, 1096, 1302, 1593
Van91I CCANNNNNTGG 1 cut(s) 1156
XapI RAATTY 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 939
XspI CTAG 3 cut(s) 507, 992, 1430
Zsp2I ATGCAT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.