Prupe.7G175800_v2.0.a1

ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015516
Physical Location & Seq
Reverse (-)
17487979 .. 17490015
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G175800.1

Sequence Viewer

Length: 1410 bp
ATGACTTATAGCAATTACTCCGGAGTTCTCCCGGTGAATCTCCTGCCAGACGAAGCATCACCACGATGGAACAACAAAGGAGACAATGGATGGCAGCTAATAGCAACCACCATGGTAGGCATGCAGAGTGTTCCTGGCCTTGTCATACTCTATGGAAGCATGGTGAAGAAGAAATGGGCTGTAAACTCAGCTTTCATGGCCCTATATGCCTTTGCTGCAGTCCTCATCTGTTGGGTTCTTTGGGCTCACAACATGTCATTTGGTACCAGATTTTGCCCAATATTGGGCAAGCCTGCTTTTGCTCTGACCCGAAGGTTTCTCCTCCATGAGTATGGAGGTTTTTATGTTCCAATGGCTGATTATGTCTACTATGAATTTGTTTTTGCTGCCATTACTGTGATATTGCTTGGGGGCTCCTTGCTTGGGAGGATGAACTTCTATGCTTGGATGTTGTTTGTGCCAATGTGGCTCACCTTGTCTTACACAGTTGGTGCTTTCAGTATTTGGGGAAAAGGGTTTCTTGAGAACTACATCATTGATTATGCTGGTGGATATGTGATTCATCTGTCATCTGGTGTGGCTGGTTTTACTGCTGCTTATTGGGTAGGACCAAGGCAATCACATGACAGGCAACATTTCCCTCCAAACAACATAATTCACATGTTGGGAGGTGCAGGGTTTTTGTGGCTTGGATGGACAGGGTTCAATGGTGGCTCTCCATTTGCAGCAAACTTGATTGCATCTTTGGCCATTATCAACACCCATATATGCACTGCCACTAGCCTCTTGGTGTGGGTTTCCCTTGATATGGTAGTGTACAAGAAAAGCTCCGTGATCGGCGCGGTCCAGGGGATGATCACTGGCCTCGTCTGCATCACACCTGGTGCAGGGATTGTGGAGCCATGGGCAGCAATAATAATGGGAGCAATGTCTGGTTCAATACCTTGGTACACCATGATGGTATTGCACAGAAGATCAGCCCTCTTCCAAAGTGTTGATGACACATTGGGGGCCTTTCACACCCACGCCGTGGCTGGCCTGTTGGGGGGTCTCCTCTCAGGCTTCTTTGCTCGAGCCAACCTTCTGAGAATGATGTACCCGACTAGCCATTACGGTCCAGGCTTAATGTACAGCATCCTTGATAGGAAACCAAAAGATGGCCTCATACAAATGGGGCACCAGATCATTGGAGCAGTTTTCATTATCGTATGGAATGTCGTTGTCACAAGCTTGATTTGCATTTTGATCAGCCGTATCGTTGATCTTAGGATGAAGGAAGAGGACCTTGACATTGGTGATGATGCTGCGCATGGAGAAGAAGCCTATGCTCTGTGGGGAGACGGTGAGAAGATGCCGATCAGATGGCAGATGCCTCCAAGGATTCCTTCCATGTGCAGACGGCTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

470

Amino Acids

51.8

Weight (kDa)

8.61

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015516)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10970
malus_domestica MD02G1122700.v1.1
prunus_persica Prupe.7G175800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0098231
rosa_laevigata RLG00000016777
rosa_multiflora Rmu_co8345397.1_g000001 Rmu_sc0009178.1_g000002 Rmu_sc0009922.1_g000007
rosa_roxburghii Rroxscaffold_2G00144140
rosa_rugosa Rorug02G0071300 Rorug02G0071400
rosa_samantha Rh2AG118400 Rh2BG121500 Rh2CG123100 Rh2DG123200
rosa_wichuraiana Rw2G009290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1308
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 263, 1176
AccB7I CCANNNNNTGG 2 cut(s) 1030, 1157
AccI GTMKAC 1 cut(s) 366
AccII CGCG 1 cut(s) 842
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 1 cut(s) 842
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 1 cut(s) 374
AdeI CACNNNGTG 2 cut(s) 884, 1030
AfaI GTAC 5 cut(s) 265, 818, 950, 1097, 1130
AfiI CCNNNNNNNGG 8 cut(s) 283, 284, 423, 808, 887, 1030, 1045, 1157
AflIII ACRYGT 2 cut(s) 252, 660
AgsI TTSAA 2 cut(s) 706, 939
AjnI CCWGG 4 cut(s) 133, 846, 880, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 1369, 1401
AluBI AGCT 4 cut(s) 97, 191, 828, 1230
AluI AGCT 4 cut(s) 97, 191, 828, 1230
Alw26I GTCTC 3 cut(s) 75, 1055, 1332
Ama87I CYCGRG 1 cut(s) 1071
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 7 cut(s) 136, 198, 747, 862, 1011, 1036, 1159
ApeKI GCWGC 7 cut(s) 94, 215, 386, 593, 725, 908, 1304
ApoI RAATTY 1 cut(s) 374
Asp718I GGTACC 1 cut(s) 263
AspLEI GCGC 2 cut(s) 842, 1309
AspS9I GGNCC 6 cut(s) 199, 608, 844, 1011, 1115, 1282
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 6 cut(s) 46, 51, 175, 463, 1307, 1355
AvaI CYCGRG 1 cut(s) 1071
AvaII GGWCC 4 cut(s) 608, 844, 1115, 1282
BaeGI GKGCMC 1 cut(s) 1179
BalI TGGCCA 1 cut(s) 749
BanI GGYRCC 2 cut(s) 263, 1176
BanII GRGCYC 2 cut(s) 247, 416
BbvI GCAGC 7 cut(s) 106, 202, 373, 580, 737, 920, 1291
BccI CCATC 6 cut(s) 60, 84, 687, 952, 1151, 1356
BceAI ACGGC 2 cut(s) 1013, 1236
BciT130I CCWGG 4 cut(s) 135, 848, 882, 1119
BclI TGATCA 2 cut(s) 855, 1245
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 3 cut(s) 75, 1055, 1332
BfaI CTAG 2 cut(s) 780, 1104
BfmI CTRYAG 1 cut(s) 216
BglI GCCNNNNNGGC 1 cut(s) 466
BisI GCNGC 7 cut(s) 95, 216, 387, 594, 726, 909, 1305
BlsI GCNGC 7 cut(s) 96, 217, 388, 595, 727, 910, 1306
Bme1390I CCNGG 5 cut(s) 32, 135, 848, 882, 1119
Bme18I GGWCC 4 cut(s) 608, 844, 1115, 1282
BmeT110I CYCGRG 1 cut(s) 1071
BmgT120I GGNCC 6 cut(s) 199, 608, 844, 1011, 1115, 1282
BmiI GGNNCC 5 cut(s) 265, 415, 900, 1012, 1178
BmrFI CCNGG 5 cut(s) 32, 135, 848, 882, 1119
BmsI GCATC 7 cut(s) 65, 749, 882, 1143, 1291, 1341, 1359
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 1355
BsaI GGTCTC 1 cut(s) 1055
BsaJI CCNNGG 7 cut(s) 111, 611, 847, 902, 944, 1029, 1376
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 8 cut(s) 283, 284, 423, 808, 887, 1030, 1045, 1157
Bse1I ACTGG 1 cut(s) 865
Bse3DI GCAATG 1 cut(s) 933
Bse8I GATNNNNATC 1 cut(s) 1355
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 4 cut(s) 135, 848, 882, 1119
BseDI CCNNGG 7 cut(s) 111, 611, 847, 902, 944, 1029, 1376
BseGI GGATG 7 cut(s) 95, 435, 453, 698, 858, 1134, 1275
BseJI GATNNNNATC 1 cut(s) 1355
BseLI CCNNNNNNNGG 8 cut(s) 283, 284, 423, 808, 887, 1030, 1045, 1157
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 3 cut(s) 201, 1071, 1076
BseNI ACTGG 1 cut(s) 865
BseRI GAGGAG 2 cut(s) 311, 1043
BseSI GKGCMC 1 cut(s) 1179
BseXI GCAGC 7 cut(s) 106, 202, 373, 580, 737, 920, 1291
BsgI GTGCAG 2 cut(s) 693, 906
Bsh1236I CGCG 1 cut(s) 842
BshFI GGCC 7 cut(s) 138, 200, 749, 864, 1013, 1038, 1161
BshNI GGYRCC 2 cut(s) 263, 1176
BsiHKCI CYCGRG 1 cut(s) 1071
BsiSI CCGG 2 cut(s) 21, 32
BslI CCNNNNNNNGG 8 cut(s) 283, 284, 423, 808, 887, 1030, 1045, 1157
BsmAI GTCTC 3 cut(s) 75, 1055, 1332
BsmBI CGTCTC 1 cut(s) 1332
BsnI GGCC 7 cut(s) 138, 200, 749, 864, 1013, 1038, 1161
Bso31I GGTCTC 1 cut(s) 1055
BsoBI CYCGRG 1 cut(s) 1071
Bsp1286I GDGCHC 3 cut(s) 247, 416, 1179
Bsp13I TCCGGA 1 cut(s) 20
Bsp1407I TGTACA 2 cut(s) 816, 1128
Bsp143I GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
Bsp19I CCATGG 2 cut(s) 111, 902
BspACI CCGC 1 cut(s) 842
BspANI GGCC 7 cut(s) 138, 200, 749, 864, 1013, 1038, 1161
BspCNI CTCAG 3 cut(s) 200, 1070, 1077
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 1 cut(s) 842
BspLI GGNNCC 5 cut(s) 265, 415, 900, 1012, 1178
BspMAI CTGCAG 1 cut(s) 220
BspT107I GGYRCC 2 cut(s) 263, 1176
BspTNI GGTCTC 1 cut(s) 1055
BsrDI GCAATG 1 cut(s) 933
BsrGI TGTACA 2 cut(s) 816, 1128
BsrI ACTGG 1 cut(s) 865
BssECI CCNNGG 7 cut(s) 111, 611, 847, 902, 944, 1029, 1376
BssMI GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
BssT1I CCWWGG 5 cut(s) 111, 611, 902, 944, 1376
Bst2UI CCWGG 4 cut(s) 135, 848, 882, 1119
Bst4CI ACNGT 4 cut(s) 397, 487, 1115, 1343
Bst6I CTCTTC 2 cut(s) 989, 1272
BstAUI TGTACA 2 cut(s) 816, 1128
BstC8I GCNNGC 4 cut(s) 122, 290, 294, 1036
BstDEI CTNAG 4 cut(s) 187, 1057, 1085, 1265
BstDSI CCRYGG 3 cut(s) 111, 902, 1029
BstENI CCTNNNNNAGG 1 cut(s) 885
BstF5I GGATG 7 cut(s) 95, 435, 453, 698, 858, 1134, 1275
BstFNI CGCG 1 cut(s) 842
BstHHI GCGC 2 cut(s) 842, 1309
BstKTI GATC 7 cut(s) 837, 858, 977, 1185, 1248, 1264, 1359
BstMAI GTCTC 3 cut(s) 75, 1055, 1332
BstMBI GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
BstMWI GCNNNNNNNGC 7 cut(s) 197, 206, 215, 466, 746, 870, 1236
BstNI CCWGG 4 cut(s) 135, 848, 882, 1119
BstNSI RCATGY 3 cut(s) 124, 256, 664
BstSCI CCNGG 5 cut(s) 30, 133, 846, 880, 1117
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 1 cut(s) 1179
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 7 cut(s) 106, 202, 373, 580, 737, 920, 1291
BstXI CCANNNNNNTGG 2 cut(s) 332, 1187
BsuRI GGCC 7 cut(s) 138, 200, 749, 864, 1013, 1038, 1161
BtgI CCRYGG 3 cut(s) 111, 902, 1029
BtsCI GGATG 7 cut(s) 95, 435, 453, 698, 858, 1134, 1275
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 2 cut(s) 771, 858
Cac8I GCNNGC 4 cut(s) 122, 290, 294, 1036
CfoI GCGC 2 cut(s) 842, 1309
Cfr13I GGNCC 6 cut(s) 199, 608, 844, 1011, 1115, 1282
CsiI ACCWGGT 1 cut(s) 880
Csp6I GTAC 5 cut(s) 264, 817, 949, 1096, 1129
CviQI GTAC 5 cut(s) 264, 817, 949, 1096, 1129
DdeI CTNAG 4 cut(s) 187, 1057, 1085, 1265
DpnI GATC 7 cut(s) 836, 857, 976, 1184, 1247, 1263, 1358
DpnII GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
DraIII CACNNNGTG 2 cut(s) 884, 1030
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 989, 1272
EarI CTCTTC 2 cut(s) 989, 1272
Eco130I CCWWGG 5 cut(s) 111, 611, 902, 944, 1376
Eco24I GRGCYC 2 cut(s) 247, 416
Eco31I GGTCTC 1 cut(s) 1055
Eco47I GGWCC 4 cut(s) 608, 844, 1115, 1282
Eco88I CYCGRG 1 cut(s) 1071
EcoNI CCTNNNNNAGG 1 cut(s) 885
EcoO109I RGGNCCY 2 cut(s) 1011, 1282
EcoRII CCWGG 4 cut(s) 133, 846, 880, 1117
EcoT14I CCWWGG 5 cut(s) 111, 611, 902, 944, 1376
EcoT38I GRGCYC 2 cut(s) 247, 416
ErhI CCWWGG 5 cut(s) 111, 611, 902, 944, 1376
Esp3I CGTCTC 1 cut(s) 1332
FalI AAGNNNNNCTT 6 cut(s) 504, 536, 1269, 1301, 1369, 1401
FbaI TGATCA 2 cut(s) 855, 1245
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 7 cut(s) 95, 216, 387, 594, 726, 909, 1305
FokI GGATG 7 cut(s) 102, 442, 460, 705, 865, 1121, 1282
FriOI GRGCYC 2 cut(s) 247, 416
Fsp4HI GCNGC 7 cut(s) 95, 216, 387, 594, 726, 909, 1305
FspBI CTAG 2 cut(s) 780, 1104
FspI TGCGCA 1 cut(s) 1308
GlaI GCGC 2 cut(s) 841, 1308
GluI GCNGC 7 cut(s) 95, 216, 387, 594, 726, 909, 1305
HaeIII GGCC 7 cut(s) 138, 200, 749, 864, 1013, 1038, 1161
HapII CCGG 2 cut(s) 21, 32
HhaI GCGC 2 cut(s) 842, 1309
Hin6I GCGC 2 cut(s) 840, 1307
HinP1I GCGC 2 cut(s) 840, 1307
HindIII AAGCTT 1 cut(s) 1228
HinfI GANTC 3 cut(s) 37, 559, 1381
HpaII CCGG 2 cut(s) 21, 32
HphI GGTGA 6 cut(s) 46, 51, 175, 463, 1307, 1355
Hpy166II GTNNAC 4 cut(s) 184, 367, 817, 951
Hpy188I TCNGA 3 cut(s) 306, 1086, 1361
Hpy188III TCNNGA 2 cut(s) 21, 521
Hpy8I GTNNAC 4 cut(s) 184, 367, 817, 951
HpyAV CCTTC 4 cut(s) 306, 1091, 1267, 1395
HpyCH4III ACNGT 4 cut(s) 397, 487, 1115, 1343
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 206, 215, 466, 746, 870, 1236
HpyF3I CTNAG 4 cut(s) 187, 1057, 1085, 1265
HspAI GCGC 2 cut(s) 840, 1307
Kpn2I TCCGGA 1 cut(s) 20
KpnI GGTACC 1 cut(s) 267
Ksp22I TGATCA 2 cut(s) 855, 1245
Kzo9I GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
LmnI GCTCC 5 cut(s) 419, 833, 898, 923, 1190
Lsp1109I GCAGC 7 cut(s) 106, 202, 373, 580, 737, 920, 1291
LweI GCATC 7 cut(s) 65, 749, 882, 1143, 1291, 1341, 1359
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 2 cut(s) 780, 1104
MaeIII GTNAC 1 cut(s) 1222
MalI GATC 7 cut(s) 836, 857, 976, 1184, 1247, 1263, 1358
MboI GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
MboII GAAGA 7 cut(s) 178, 181, 976, 984, 1289, 1328, 1360
MhlI GDGCHC 3 cut(s) 247, 416, 1179
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 4 cut(s) 13, 374, 654, 1405
MluNI TGGCCA 1 cut(s) 749
Mox20I TGGCCA 1 cut(s) 749
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 749
MseI TTAA 2 cut(s) 1124, 1408
MslI CAYNNNNRTG 5 cut(s) 64, 330, 395, 956, 1169
Msp20I TGGCCA 1 cut(s) 749
MspI CCGG 2 cut(s) 21, 32
MspR9I CCNGG 5 cut(s) 32, 135, 848, 882, 1119
MvaI CCWGG 4 cut(s) 135, 848, 882, 1119
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 7 cut(s) 197, 206, 215, 466, 746, 870, 1236
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 2 cut(s) 111, 902
NdeII GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
NlaIV GGNNCC 5 cut(s) 265, 415, 900, 1012, 1178
NmuCI GTSAC 1 cut(s) 1222
NsbI TGCGCA 1 cut(s) 1308
NspI RCATGY 3 cut(s) 124, 256, 664
PaeI GCATGC 1 cut(s) 124
PaeR7I CTCGAG 1 cut(s) 1071
PciI ACATGT 2 cut(s) 252, 660
PfeI GAWTC 3 cut(s) 37, 559, 1381
PflMI CCANNNNNTGG 2 cut(s) 1030, 1157
PkrI GCNGC 7 cut(s) 96, 217, 388, 595, 727, 910, 1306
PpuMI RGGWCCY 1 cut(s) 1282
PscI ACATGT 2 cut(s) 252, 660
Psp5II RGGWCCY 1 cut(s) 1282
Psp6I CCWGG 4 cut(s) 133, 846, 880, 1117
PspGI CCWGG 4 cut(s) 133, 846, 880, 1117
PspN4I GGNNCC 5 cut(s) 265, 415, 900, 1012, 1178
PspPI GGNCC 6 cut(s) 199, 608, 844, 1011, 1115, 1282
PspPPI RGGWCCY 1 cut(s) 1282
PspXI VCTCGAGB 1 cut(s) 1071
PstI CTGCAG 1 cut(s) 220
RsaI GTAC 5 cut(s) 265, 818, 950, 1097, 1130
RsaNI GTAC 5 cut(s) 264, 817, 949, 1096, 1129
RseI CAYNNNNRTG 5 cut(s) 64, 330, 395, 956, 1169
SaqAI TTAA 2 cut(s) 1124, 1408
SatI GCNGC 7 cut(s) 95, 216, 387, 594, 726, 909, 1305
Sau3AI GATC 7 cut(s) 834, 855, 974, 1182, 1245, 1261, 1356
Sau96I GGNCC 6 cut(s) 199, 608, 844, 1011, 1115, 1282
ScrFI CCNGG 5 cut(s) 32, 135, 848, 882, 1119
SduI GDGCHC 3 cut(s) 247, 416, 1179
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 7 cut(s) 65, 749, 882, 1143, 1291, 1341, 1359
SfcI CTRYAG 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 1071
SinI GGWCC 4 cut(s) 608, 844, 1115, 1282
SlaI CTCGAG 1 cut(s) 1071
SmiMI CAYNNNNRTG 5 cut(s) 64, 330, 395, 956, 1169
SmlI CTYRAG 2 cut(s) 521, 1071
SmoI CTYRAG 2 cut(s) 521, 1071
SphI GCATGC 1 cut(s) 124
Sse9I AATT 4 cut(s) 13, 374, 654, 1405
SsiI CCGC 1 cut(s) 842
SspI AATATT 1 cut(s) 282
SspMI CTAG 2 cut(s) 780, 1104
StyD4I CCNGG 5 cut(s) 30, 133, 846, 880, 1117
StyI CCWWGG 5 cut(s) 111, 611, 902, 944, 1376
TaaI ACNGT 4 cut(s) 397, 487, 1115, 1343
TaqI TCGA 1 cut(s) 1072
TasI AATT 4 cut(s) 13, 374, 654, 1405
TatI WGTACW 2 cut(s) 816, 1128
TfiI GAWTC 3 cut(s) 37, 559, 1381
Tru1I TTAA 2 cut(s) 1124, 1408
Tru9I TTAA 2 cut(s) 1124, 1408
TscAI CASTG 2 cut(s) 778, 865
TseFI GTSAC 1 cut(s) 1222
TseI GCWGC 7 cut(s) 94, 215, 386, 593, 725, 908, 1304
Tsp45I GTSAC 1 cut(s) 1222
TspDTI ATGAA 6 cut(s) 184, 387, 446, 551, 1189, 1286
TspGWI ACGGA 1 cut(s) 820
TspRI CASTG 2 cut(s) 778, 865
Van91I CCANNNNNTGG 2 cut(s) 1030, 1157
VpaK11BI GGWCC 4 cut(s) 608, 844, 1115, 1282
XagI CCTNNNNNAGG 1 cut(s) 885
XapI RAATTY 1 cut(s) 374
XceI RCATGY 3 cut(s) 124, 256, 664
XcmI CCANNNNNNNNNTGG 2 cut(s) 784, 1031
XhoI CTCGAG 1 cut(s) 1071
XmiI GTMKAC 1 cut(s) 366
XspI CTAG 2 cut(s) 780, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.