Bifunctional nuclease
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G66050 AT5G66050 AT5G66050 AT5G66050 |
| fragaria_vesca | FvH4_1g10980 FvH4_1g10980 FvH4_1g10980 FvH4_1g10980 |
| malus_domestica | MD02G1122800.v1.1 MD15G1236800.v1.1 |
| prunus_persica | Prupe.7G175700_v2.0.a1 |
| pyrus_communis | pycom02g09580 pycom15g21060 |
| rosa_chinensis | RchiOBHm_Chr2g0098241 |
| rosa_laevigata | RLG00000016778 |
| rosa_multiflora | Rmu_co8414957.1_g000001 Rmu_sc0009178.1_g000001 |
| rosa_roxburghii | Rroxscaffold_2G00144130 |
| rosa_rugosa | Rorug02G0071400 Rorug02G0071500 |
| rosa_samantha | Rh2AG118500 Rh2BG121600 Rh2CG123200 |
| rosa_wichuraiana | Rw2G009300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 752 |
| Acc36I | ACCTGC | 1 cut(s) | 263 |
| AccII | CGCG | 1 cut(s) | 753 |
| AclWI | GGATC | 1 cut(s) | 794 |
| AcsI | RAATTY | 2 cut(s) | 28, 76 |
| AcuI | CTGAAG | 1 cut(s) | 693 |
| AfaI | GTAC | 2 cut(s) | 188, 259 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 272, 383, 529 |
| AgsI | TTSAA | 3 cut(s) | 298, 326, 604 |
| AluBI | AGCT | 4 cut(s) | 280, 452, 491, 540 |
| AluI | AGCT | 4 cut(s) | 280, 452, 491, 540 |
| Alw26I | GTCTC | 1 cut(s) | 489 |
| AlwI | GGATC | 1 cut(s) | 794 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 266 |
| ApoI | RAATTY | 2 cut(s) | 28, 76 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 536, 568 |
| AspLEI | GCGC | 1 cut(s) | 804 |
| AsuHPI | GGTGA | 2 cut(s) | 230, 791 |
| AxyI | CCTNAGG | 1 cut(s) | 695 |
| BanII | GRGCYC | 1 cut(s) | 693 |
| BbsI | GAAGAC | 1 cut(s) | 182 |
| BccI | CCATC | 3 cut(s) | 217, 377, 776 |
| BceAI | ACGGC | 1 cut(s) | 378 |
| BciVI | GTATCC | 1 cut(s) | 338 |
| BcoDI | GTCTC | 1 cut(s) | 489 |
| BfaI | CTAG | 1 cut(s) | 108 |
| BfmI | CTRYAG | 1 cut(s) | 12 |
| BfuAI | ACCTGC | 1 cut(s) | 263 |
| BfuI | GTATCC | 1 cut(s) | 338 |
| BmsI | GCATC | 3 cut(s) | 127, 259, 550 |
| BpiI | GAAGAC | 1 cut(s) | 182 |
| BpmI | CTGGAG | 2 cut(s) | 201, 471 |
| BpuEI | CTTGAG | 1 cut(s) | 194 |
| BsaBI | GATNNNNATC | 1 cut(s) | 443 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 645, 675 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 272, 383, 529 |
| Bse1I | ACTGG | 4 cut(s) | 41, 184, 346, 584 |
| Bse21I | CCTNAGG | 1 cut(s) | 695 |
| Bse3DI | GCAATG | 1 cut(s) | 102 |
| Bse8I | GATNNNNATC | 1 cut(s) | 443 |
| BseGI | GGATG | 3 cut(s) | 118, 228, 685 |
| BseJI | GATNNNNATC | 1 cut(s) | 443 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 272, 383, 529 |
| BseMI | GCAATG | 1 cut(s) | 102 |
| BseNI | ACTGG | 4 cut(s) | 41, 184, 346, 584 |
| BseRI | GAGGAG | 1 cut(s) | 347 |
| BsgI | GTGCAG | 1 cut(s) | 105 |
| Bsh1236I | CGCG | 1 cut(s) | 753 |
| Bsh1285I | CGRYCG | 1 cut(s) | 412 |
| BsiEI | CGRYCG | 1 cut(s) | 412 |
| BslFI | GGGAC | 2 cut(s) | 670, 748 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 272, 383, 529 |
| BsmAI | GTCTC | 1 cut(s) | 489 |
| BsmFI | GGGAC | 2 cut(s) | 670, 748 |
| Bsp1286I | GDGCHC | 1 cut(s) | 693 |
| Bsp143I | GATC | 1 cut(s) | 786 |
| BspFNI | CGCG | 1 cut(s) | 753 |
| BspMAI | CTGCAG | 1 cut(s) | 16 |
| BspMI | ACCTGC | 1 cut(s) | 263 |
| BspPI | GGATC | 1 cut(s) | 794 |
| BsrDI | GCAATG | 1 cut(s) | 102 |
| BsrI | ACTGG | 4 cut(s) | 41, 184, 346, 584 |
| BssMI | GATC | 1 cut(s) | 786 |
| Bst4CI | ACNGT | 1 cut(s) | 661 |
| Bst6I | CTCTTC | 2 cut(s) | 357, 669 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 269 |
| BstC8I | GCNNGC | 2 cut(s) | 454, 800 |
| BstDEI | CTNAG | 2 cut(s) | 514, 695 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 270 |
| BstF5I | GGATG | 3 cut(s) | 118, 228, 685 |
| BstFNI | CGCG | 1 cut(s) | 753 |
| BstHHI | GCGC | 1 cut(s) | 804 |
| BstKTI | GATC | 1 cut(s) | 789 |
| BstMAI | GTCTC | 1 cut(s) | 489 |
| BstMBI | GATC | 1 cut(s) | 786 |
| BstMCI | CGRYCG | 1 cut(s) | 412 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 92, 269 |
| BstSFI | CTRYAG | 1 cut(s) | 12 |
| BstUI | CGCG | 1 cut(s) | 753 |
| BstV2I | GAAGAC | 1 cut(s) | 182 |
| Bsu36I | CCTNAGG | 1 cut(s) | 695 |
| BsuI | GTATCC | 1 cut(s) | 338 |
| BtsCI | GGATG | 3 cut(s) | 118, 228, 685 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 1 cut(s) | 93 |
| BveI | ACCTGC | 1 cut(s) | 263 |
| Cac8I | GCNNGC | 2 cut(s) | 454, 800 |
| CaiI | CAGNNNCTG | 1 cut(s) | 266 |
| CfoI | GCGC | 1 cut(s) | 804 |
| CseI | GACGC | 1 cut(s) | 742 |
| Csp6I | GTAC | 2 cut(s) | 187, 258 |
| CviAII | CATG | 1 cut(s) | 289 |
| CviQI | GTAC | 2 cut(s) | 187, 258 |
| DdeI | CTNAG | 2 cut(s) | 514, 695 |
| DpnI | GATC | 1 cut(s) | 788 |
| DpnII | GATC | 1 cut(s) | 786 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 752 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 752 |
| Eam1104I | CTCTTC | 2 cut(s) | 357, 669 |
| EarI | CTCTTC | 2 cut(s) | 357, 669 |
| Eco24I | GRGCYC | 1 cut(s) | 693 |
| Eco57I | CTGAAG | 1 cut(s) | 693 |
| Eco81I | CCTNAGG | 1 cut(s) | 695 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 270 |
| EcoRI | GAATTC | 1 cut(s) | 76 |
| EcoT38I | GRGCYC | 1 cut(s) | 693 |
| FaeI | CATG | 1 cut(s) | 292 |
| FaqI | GGGAC | 2 cut(s) | 670, 748 |
| FatI | CATG | 1 cut(s) | 288 |
| FauNDI | CATATG | 1 cut(s) | 421 |
| FokI | GGATG | 3 cut(s) | 105, 235, 692 |
| FriOI | GRGCYC | 1 cut(s) | 693 |
| FspBI | CTAG | 1 cut(s) | 108 |
| GlaI | GCGC | 1 cut(s) | 803 |
| GsuI | CTGGAG | 2 cut(s) | 201, 471 |
| HgaI | GACGC | 1 cut(s) | 742 |
| HhaI | GCGC | 1 cut(s) | 804 |
| Hin1II | CATG | 1 cut(s) | 292 |
| Hin6I | GCGC | 1 cut(s) | 802 |
| HinP1I | GCGC | 1 cut(s) | 802 |
| HinfI | GANTC | 2 cut(s) | 373, 482 |
| HphI | GGTGA | 2 cut(s) | 230, 791 |
| Hpy166II | GTNNAC | 2 cut(s) | 126, 187 |
| Hpy188III | TCNNGA | 3 cut(s) | 295, 377, 503 |
| Hpy8I | GTNNAC | 2 cut(s) | 126, 187 |
| Hpy99I | CGWCG | 1 cut(s) | 758 |
| HpyAV | CCTTC | 2 cut(s) | 523, 527 |
| HpyCH4III | ACNGT | 1 cut(s) | 661 |
| HpyCH4V | TGCA | 8 cut(s) | 14, 52, 86, 145, 233, 272, 686, 798 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 92, 269 |
| HpyF3I | CTNAG | 2 cut(s) | 514, 695 |
| Hsp92II | CATG | 1 cut(s) | 292 |
| HspAI | GCGC | 1 cut(s) | 802 |
| Kzo9I | GATC | 1 cut(s) | 786 |
| LmnI | GCTCC | 2 cut(s) | 4, 537 |
| LweI | GCATC | 3 cut(s) | 127, 259, 550 |
| MaeI | CTAG | 1 cut(s) | 108 |
| MaeIII | GTNAC | 1 cut(s) | 236 |
| MalI | GATC | 1 cut(s) | 788 |
| MboI | GATC | 1 cut(s) | 786 |
| MboII | GAAGA | 3 cut(s) | 187, 344, 686 |
| MfeI | CAATTG | 1 cut(s) | 321 |
| MhlI | GDGCHC | 1 cut(s) | 693 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MseI | TTAA | 3 cut(s) | 218, 612, 726 |
| MspA1I | CMGCKG | 1 cut(s) | 280 |
| MunI | CAATTG | 1 cut(s) | 321 |
| MvnI | CGCG | 1 cut(s) | 753 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 92, 269 |
| NdeI | CATATG | 1 cut(s) | 421 |
| NdeII | GATC | 1 cut(s) | 786 |
| NlaIII | CATG | 1 cut(s) | 292 |
| NmuCI | GTSAC | 1 cut(s) | 236 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 368 |
| PfeI | GAWTC | 1 cut(s) | 373 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| PstI | CTGCAG | 1 cut(s) | 16 |
| PstNI | CAGNNNCTG | 1 cut(s) | 266 |
| PvuII | CAGCTG | 1 cut(s) | 280 |
| RsaI | GTAC | 2 cut(s) | 188, 259 |
| RsaNI | GTAC | 2 cut(s) | 187, 258 |
| SaqAI | TTAA | 3 cut(s) | 218, 612, 726 |
| Sau3AI | GATC | 1 cut(s) | 786 |
| SchI | GAGTC | 1 cut(s) | 491 |
| SduI | GDGCHC | 1 cut(s) | 693 |
| SfaNI | GCATC | 3 cut(s) | 127, 259, 550 |
| SfcI | CTRYAG | 1 cut(s) | 12 |
| SmlI | CTYRAG | 1 cut(s) | 209 |
| SmoI | CTYRAG | 1 cut(s) | 209 |
| SspMI | CTAG | 1 cut(s) | 108 |
| TaaI | ACNGT | 1 cut(s) | 661 |
| TaqI | TCGA | 3 cut(s) | 408, 543, 756 |
| TaqII | GACCGA | 1 cut(s) | 426 |
| TfiI | GAWTC | 1 cut(s) | 373 |
| Tru1I | TTAA | 3 cut(s) | 218, 612, 726 |
| Tru9I | TTAA | 3 cut(s) | 218, 612, 726 |
| TscAI | CASTG | 1 cut(s) | 93 |
| TseFI | GTSAC | 1 cut(s) | 236 |
| Tsp45I | GTSAC | 1 cut(s) | 236 |
| TspDTI | ATGAA | 4 cut(s) | 188, 438, 466, 703 |
| TspGWI | ACGGA | 2 cut(s) | 111, 718 |
| TspRI | CASTG | 1 cut(s) | 93 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 270 |
| XapI | RAATTY | 2 cut(s) | 28, 76 |
| XspI | CTAG | 1 cut(s) | 108 |