Rmu_co8414957.1_g000001

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8414957.1
OG0007911
Physical Location & Seq
Reverse (-)
1 .. 1248
1248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8414957.1_g000001.1.cds

Sequence Viewer

Length: 386 bp
atggtgagaattacagaaagagtgctgaatacttactttgccagattgtattttagcaaggtggtggtggtaaaattcccaggcaaaagtgacattcttagtgtggatgcacgtccatcagatgccataaatgttgcatatagatgcaaggcacccatatatgtaaataaacagattgttttaaaggatgctgtaagactcagttatgggatgggcagaatgcgtgatatgaaatctacttacgatgtctctcttgatagtgctgctgatggtcccaattttttagctcaagaacttgttttggtgaagaagatcgaattagctatcaaagaggaaagatataaggatgcagccatgtggagagacgaactaatcaaacttcgcaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

128

Amino Acids

14.71

Weight (kDa)

9.57

Isoelectric Point (pI)

22.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 151
AcsI RAATTY 1 cut(s) 74
AjiI CACGTC 1 cut(s) 113
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 2 cut(s) 287, 323
AluI AGCT 2 cut(s) 287, 323
Alw26I GTCTC 2 cut(s) 253, 357
ApeKI GCWGC 2 cut(s) 263, 350
ApoI RAATTY 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 16, 316
AvaII GGWCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 250, 362
BccI CCATC 3 cut(s) 124, 205, 263
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 253, 357
BisI GCNGC 2 cut(s) 264, 351
BlsI GCNGC 2 cut(s) 265, 352
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 2 cut(s) 153, 274
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 5 cut(s) 97, 112, 134, 178, 337
BpuEI CTTGAG 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 79
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 4 cut(s) 112, 193, 216, 352
BseMII CTCAG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 250, 362
BshNI GGYRCC 1 cut(s) 151
BslFI GGGAC 1 cut(s) 258
BsmAI GTCTC 2 cut(s) 253, 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 258
BsmI GAATGC 1 cut(s) 225
Bsp143I GATC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 153, 274
BspT107I GGYRCC 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 98, 200
BstF5I GGATG 4 cut(s) 112, 193, 216, 352
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 253, 357
BstMBI GATC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 79
BstV1I GCAGC 2 cut(s) 250, 362
BtrI CACGTC 1 cut(s) 113
BtsCI GGATG 4 cut(s) 112, 193, 216, 352
Cfr13I GGNCC 1 cut(s) 272
CviAII CATG 1 cut(s) 355
CviJI RGCY 3 cut(s) 287, 323, 353
CviKI_1 RGCY 3 cut(s) 287, 323, 353
DdeI CTNAG 2 cut(s) 98, 200
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
DraI TTTAAA 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 79
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 1 cut(s) 358
FaqI GGGAC 1 cut(s) 258
FatI CATG 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 264, 351
FokI GGATG 4 cut(s) 119, 200, 223, 359
Fsp4HI GCNGC 2 cut(s) 264, 351
GluI GCNGC 2 cut(s) 264, 351
Hin1II CATG 1 cut(s) 358
HinfI GANTC 1 cut(s) 198
HphI GGTGA 2 cut(s) 16, 316
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 2 cut(s) 254, 290
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 110, 137, 147, 350
HpyF3I CTNAG 2 cut(s) 98, 200
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 358
Kzo9I GATC 1 cut(s) 312
LpnPI CCDG 3 cut(s) 55, 66, 93
Lsp1109I GCAGC 2 cut(s) 250, 362
LweI GCATC 5 cut(s) 97, 112, 134, 178, 337
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 89
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 2 cut(s) 319, 322
MluCI AATT 4 cut(s) 9, 74, 277, 317
MlyI GAGTC 1 cut(s) 192
MnlI CCTC 1 cut(s) 325
MseI TTAA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 1 cut(s) 81
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 2 cut(s) 153, 274
NmuCI GTSAC 1 cut(s) 89
PctI GAATGC 1 cut(s) 225
PkrI GCNGC 2 cut(s) 265, 352
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 153, 274
PspPI GGNCC 1 cut(s) 272
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 1 cut(s) 182
SatI GCNGC 2 cut(s) 264, 351
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 1 cut(s) 81
SetI ASST 4 cut(s) 63, 115, 289, 325
SfaNI GCATC 5 cut(s) 97, 112, 134, 178, 337
SinI GGWCC 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 4 cut(s) 9, 74, 277, 317
StyD4I CCNGG 1 cut(s) 79
TaiI ACGT 1 cut(s) 115
TaqI TCGA 1 cut(s) 315
TasI AATT 4 cut(s) 9, 74, 277, 317
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 2 cut(s) 263, 350
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 272
XapI RAATTY 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.