FvH4_1g10980

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007911
Physical Location & Seq
Reverse (-)
5973501 .. 5976418
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10980.t4

Sequence Viewer

Length: 984 bp
ATGATGCTCGGAGCTGCTCAATTCTCCGTCGCCGATCGCTCGAATGCCGCTTTCCCGATCCCAAATACTCGCCCAGCGCTTTCCTCTTCTTCTTCCTCTTCCTCTCACACCTTAGATTCCGTCGTCTTCCGGTTAGGTTTCCGAGCCAAACGCCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCGACTCCTCCTCTCGCGACCGATCCACTAATCAGCAAGATTACGAGTACATTCAAGCTTCCCTCCTGCTTTCAGAAACTATATCTCACTACCGAATGCAAAAACGAGGGTTTCAAGAAGAGACGAATTGGAATACCTCCAGAAAACTGCGGCCGTTTTCGGTGCAAGCAACCCGGCCCCGGAGTGTTTTGGATAAACTAGGCAGGAACTTCCTCCAGCGATTCCAAAGTCCTACTATTTTCCTCAAGATATCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGATAGAGAATCTCATTAATGGCCACTGGGGAGATGAGAATGGGGATTCCCCGGACCAGTTTCAGTTTGTGAAAAGTCTTGTTGAAGAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTGCTGCATACTTACTTTGCCAGATTGTATTTTAGTAAGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCCCGTCCATCAGATGCCATAAATGTTGCATACAGGTGCAAGGCACCCATATATGTAAATAAACAGATTGTTGTAAGAGATGCTGTAAGAATCAGTTATGGGATGGGCAGAATGCGTGAGATGAAGCCTACTTACGATGTCTCTCTTGATAGTGCTGCAGATGGTCCCAATTTATTAGCTCAAGAACTTGTTTTGGTGAAGAATATCGACTTGGCTATCAAAGAGGAAAGATATAAGGATGCAGCCATGTGGAGAGATGAACTAGTCAAACTTCGCAAGTTAAGTCAAGGGCATCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

328

Amino Acids

37.24

Weight (kDa)

9.51

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 150 - 247 1.9e-14 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 724
AccII CGCG 1 cut(s) 204
AciI CCGC 4 cut(s) 48, 154, 178, 337
AclWI GGATC 3 cut(s) 52, 169, 204
AcoI YGGCCR 2 cut(s) 338, 505
AfaI GTAC 1 cut(s) 236
AfeI AGCGCT 1 cut(s) 78
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 3 cut(s) 242, 302, 569
AhlI ACTAGT 1 cut(s) 943
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
AluBI AGCT 3 cut(s) 14, 245, 860
AluI AGCT 3 cut(s) 14, 245, 860
Alw26I GTCTC 3 cut(s) 302, 444, 826
AlwI GGATC 3 cut(s) 52, 169, 204
Aor51HI AGCGCT 1 cut(s) 78
AoxI GGCC 3 cut(s) 338, 362, 505
ApeKI GCWGC 4 cut(s) 14, 616, 836, 923
AseI ATTAAT 1 cut(s) 501
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 363, 538, 845
AsuC2I CCSGG 3 cut(s) 361, 367, 536
AsuHPI GGTGA 2 cut(s) 607, 889
AvaII GGWCC 2 cut(s) 538, 845
BalI TGGCCA 1 cut(s) 507
BanI GGYRCC 1 cut(s) 724
BarI GAAGNNNNNNTAC 2 cut(s) 797, 829
BbsI GAAGAC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 603, 823, 935
BccI CCATC 4 cut(s) 588, 697, 778, 836
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 654
BcnI CCSGG 3 cut(s) 361, 367, 536
BcoDI GTCTC 3 cut(s) 302, 444, 826
BcuI ACTAGT 1 cut(s) 943
BfaI CTAG 2 cut(s) 386, 944
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 7 cut(s) 15, 48, 154, 338, 617, 837, 924
BlsI GCNGC 7 cut(s) 16, 49, 155, 339, 618, 838, 925
Bme1390I CCNGG 4 cut(s) 361, 367, 536, 654
Bme18I GGWCC 2 cut(s) 538, 845
BmgT120I GGNCC 3 cut(s) 363, 538, 845
BmiI GGNNCC 3 cut(s) 365, 726, 847
BmrFI CCNGG 4 cut(s) 361, 367, 536, 654
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 4 cut(s) 670, 685, 751, 910
BmuI ACTGGG 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 118
BpmI CTGGAG 2 cut(s) 310, 386
BpuEI CTTGAG 2 cut(s) 416, 846
BpuMI CCSGG 3 cut(s) 361, 367, 536
Bsa29I ATCGAT 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 160, 365, 534
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 515, 541
BseBI CCWGG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 160, 365, 534
BseGI GGATG 3 cut(s) 685, 789, 925
BseLI CCNNNNNNNGG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 515, 541
BseRI GAGGAG 2 cut(s) 184, 187
BseX3I CGGCCG 1 cut(s) 338
BseXI GCAGC 3 cut(s) 603, 823, 935
BseYI CCCAGC 1 cut(s) 73
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 3 cut(s) 37, 208, 341
BshFI GGCC 3 cut(s) 340, 364, 507
BshNI GGYRCC 1 cut(s) 724
BshVI ATCGAT 1 cut(s) 173
BsiEI CGRYCG 3 cut(s) 37, 208, 341
BsiSI CCGG 4 cut(s) 130, 361, 367, 536
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 3 cut(s) 302, 444, 826
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 831
BsmI GAATGC 3 cut(s) 49, 288, 798
BsnI GGCC 3 cut(s) 340, 364, 507
Bsp143I GATC 4 cut(s) 34, 57, 174, 209
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 4 cut(s) 48, 154, 178, 337
BspANI GGCC 3 cut(s) 340, 364, 507
BspDI ATCGAT 1 cut(s) 173
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 365, 726, 847
BspMAI CTGCAG 1 cut(s) 841
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 3 cut(s) 52, 169, 204
BspT107I GGYRCC 1 cut(s) 724
BsrI ACTGG 2 cut(s) 515, 541
BssECI CCNNGG 3 cut(s) 160, 365, 534
BssMI GATC 4 cut(s) 34, 57, 174, 209
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 3 cut(s) 91, 103, 300
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 112, 671
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 3 cut(s) 685, 789, 925
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 4 cut(s) 37, 60, 177, 212
BstMAI GTCTC 3 cut(s) 302, 444, 826
BstMBI GATC 4 cut(s) 34, 57, 174, 209
BstMCI CGRYCG 3 cut(s) 37, 208, 341
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 4 cut(s) 359, 365, 534, 652
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 603, 823, 935
BstV2I GAAGAC 1 cut(s) 118
BstZI CGGCCG 1 cut(s) 338
Bsu15I ATCGAT 1 cut(s) 173
BsuRI GGCC 3 cut(s) 340, 364, 507
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 3 cut(s) 685, 789, 925
BtsIMutI CAGTG 1 cut(s) 508
BtuMI TCGCGA 1 cut(s) 204
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 363, 538, 845
ClaI ATCGAT 1 cut(s) 173
Csp6I GTAC 1 cut(s) 235
CviAII CATG 1 cut(s) 928
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 112, 671
DpnI GATC 4 cut(s) 36, 59, 176, 211
DpnII GATC 4 cut(s) 34, 57, 174, 209
EaeI YGGCCR 2 cut(s) 338, 505
EagI CGGCCG 1 cut(s) 338
Eam1104I CTCTTC 3 cut(s) 91, 103, 300
EarI CTCTTC 3 cut(s) 91, 103, 300
EclXI CGGCCG 1 cut(s) 338
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 538, 845
Eco47III AGCGCT 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 438
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 1 cut(s) 931
FaqI GGGAC 1 cut(s) 831
FatI CATG 1 cut(s) 927
Fnu4HI GCNGC 7 cut(s) 15, 48, 154, 338, 617, 837, 924
FokI GGATG 3 cut(s) 692, 796, 932
Fsp4HI GCNGC 7 cut(s) 15, 48, 154, 338, 617, 837, 924
FspBI CTAG 2 cut(s) 386, 944
GlaI GCGC 1 cut(s) 78
GluI GCNGC 7 cut(s) 15, 48, 154, 338, 617, 837, 924
GsaI CCCAGC 1 cut(s) 77
GsuI CTGGAG 2 cut(s) 310, 386
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 3 cut(s) 340, 364, 507
HapII CCGG 4 cut(s) 130, 361, 367, 536
HhaI GCGC 1 cut(s) 79
Hin1II CATG 1 cut(s) 931
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 7 cut(s) 116, 165, 191, 408, 493, 530, 771
HpaII CCGG 4 cut(s) 130, 361, 367, 536
HphI GGTGA 2 cut(s) 607, 889
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 4 cut(s) 11, 143, 262, 694
Hpy188III TCNNGA 7 cut(s) 55, 203, 302, 327, 433, 827, 863
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 2 cut(s) 32, 125
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4V TGCA 7 cut(s) 286, 352, 619, 710, 720, 839, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 2 cut(s) 112, 671
Hsp92II CATG 1 cut(s) 931
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 4 cut(s) 34, 57, 174, 209
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 3 cut(s) 603, 823, 935
LweI GCATC 4 cut(s) 670, 685, 751, 910
MaeI CTAG 2 cut(s) 386, 944
MaeIII GTNAC 2 cut(s) 443, 662
MalI GATC 4 cut(s) 36, 59, 176, 211
MboI GATC 4 cut(s) 34, 57, 174, 209
MboII GAAGA 8 cut(s) 78, 81, 84, 90, 118, 317, 581, 892
MfeI CAATTG 1 cut(s) 465
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 5 cut(s) 20, 313, 465, 600, 850
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 2 cut(s) 159, 185
Mox20I TGGCCA 1 cut(s) 507
MscI TGGCCA 1 cut(s) 507
MseI TTAA 3 cut(s) 501, 962, 982
MslI CAYNNNNRTG 1 cut(s) 715
Msp20I TGGCCA 1 cut(s) 507
MspI CCGG 4 cut(s) 130, 361, 367, 536
MspR9I CCNGG 4 cut(s) 361, 367, 536, 654
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 3 cut(s) 49, 288, 798
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 186
NciI CCSGG 3 cut(s) 361, 367, 536
NdeII GATC 4 cut(s) 34, 57, 174, 209
NlaIII CATG 1 cut(s) 931
NlaIV GGNNCC 3 cut(s) 365, 726, 847
NmuCI GTSAC 2 cut(s) 443, 662
NruI TCGCGA 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 3 cut(s) 49, 288, 798
PfeI GAWTC 5 cut(s) 116, 408, 493, 530, 771
PkrI GCNGC 7 cut(s) 16, 49, 155, 339, 618, 838, 925
Ple19I CGATCG 1 cut(s) 37
PleI GAGTC 2 cut(s) 159, 185
PpsI GAGTC 2 cut(s) 159, 185
PshBI ATTAAT 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 3 cut(s) 365, 726, 847
PspPI GGNCC 3 cut(s) 363, 538, 845
PstI CTGCAG 1 cut(s) 841
PvuI CGATCG 1 cut(s) 37
RruI TCGCGA 1 cut(s) 204
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 715
SaqAI TTAA 3 cut(s) 501, 962, 982
SatI GCNGC 7 cut(s) 15, 48, 154, 338, 617, 837, 924
Sau3AI GATC 4 cut(s) 34, 57, 174, 209
Sau96I GGNCC 3 cut(s) 363, 538, 845
SchI GAGTC 2 cut(s) 159, 185
ScrFI CCNGG 4 cut(s) 361, 367, 536, 654
SetI ASST 8 cut(s) 16, 113, 139, 188, 247, 326, 719, 862
SfaNI GCATC 4 cut(s) 670, 685, 751, 910
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 2 cut(s) 538, 845
SmiMI CAYNNNNRTG 1 cut(s) 715
SmlI CTYRAG 2 cut(s) 431, 861
SmoI CTYRAG 2 cut(s) 431, 861
SpeI ACTAGT 1 cut(s) 943
Sse9I AATT 5 cut(s) 20, 313, 465, 600, 850
SsiI CCGC 4 cut(s) 48, 154, 178, 337
SspMI CTAG 2 cut(s) 386, 944
StyD4I CCNGG 4 cut(s) 359, 365, 534, 652
TaaI ACNGT 1 cut(s) 161
TaqI TCGA 3 cut(s) 41, 173, 888
TaqII GACCGA 1 cut(s) 222
TasI AATT 5 cut(s) 20, 313, 465, 600, 850
TatI WGTACW 1 cut(s) 234
TauI GCSGC 3 cut(s) 50, 156, 340
TfiI GAWTC 5 cut(s) 116, 408, 493, 530, 771
Tru1I TTAA 3 cut(s) 501, 962, 982
Tru9I TTAA 3 cut(s) 501, 962, 982
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 2 cut(s) 443, 662
TseI GCWGC 4 cut(s) 14, 616, 836, 923
Tsp45I GTSAC 2 cut(s) 443, 662
TspDTI ATGAA 2 cut(s) 818, 954
TspGWI ACGGA 3 cut(s) 16, 109, 462
TspRI CASTG 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 538, 845
VspI ATTAAT 1 cut(s) 501
XspI CTAG 2 cut(s) 386, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.