Rmu_sc0009178.1_g000001

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009178.1
OG0007911
Physical Location & Seq
Forward (+)
132 .. 2102
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009178.1_g000001.1.cds

Sequence Viewer

Length: 696 bp
atgcaaaagagaggatttcaacaagagacgaattggaattcctctgctaaattgcggccattttcggtacgagccacccgcccccggaatgtattggatacactcgacaggaactttctccaccgtttccaaagtcctaccattttcctcaagatttcctgtgacggagactttttgcttccaattgttgtaggcgaacttgcgatagagaatctcataaatggccactggggagatgaatttggggattccccagaccagtttcagtttgtgaaaaatcttgtcgaaaaacttgaccatcaagtgaatatggtgagaattacagaaagagtgctgaatacttactttgccagattgtattttagcaagccaggcaaaagtgacattcttagtgtggatgcacgtccatcagatgccataaatgttgcatatagatgcaaggcacccatatatgtaaataaacagattgttttaaaggatgctgtaagactcagttatgggatgggcagaatgcgtgatatgaaatctacttacgatgtctctcttgatagtgctgctgatggtcccaattttttagctcaagaacttgttttggtgaagaagatcgaattagctatcaaagaggaaagatataaggatgcagccatgtggagagacgaactaatcaaacttcgcaagttaagtcaagggcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

231

Amino Acids

26.61

Weight (kDa)

9.26

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AciI CCGC 2 cut(s) 55, 79
AcoI YGGCCR 2 cut(s) 56, 223
AcsI RAATTY 2 cut(s) 37, 239
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 84
AgsI TTSAA 1 cut(s) 20
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 578, 614
AluI AGCT 2 cut(s) 578, 614
Alw26I GTCTC 4 cut(s) 20, 162, 544, 648
AoxI GGCC 2 cut(s) 56, 223
ApeKI GCWGC 2 cut(s) 554, 641
ApoI RAATTY 2 cut(s) 37, 239
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 2 cut(s) 325, 607
AvaII GGWCC 1 cut(s) 563
BalI TGGCCA 1 cut(s) 225
BanI GGYRCC 1 cut(s) 442
BbvI GCAGC 2 cut(s) 541, 653
BccI CCATC 4 cut(s) 306, 415, 496, 554
BciT130I CCWGG 1 cut(s) 372
BciVI GTATCC 1 cut(s) 91
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 4 cut(s) 20, 162, 544, 648
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 3 cut(s) 56, 555, 642
BlsI GCNGC 3 cut(s) 57, 556, 643
Bme1390I CCNGG 2 cut(s) 85, 372
Bme18I GGWCC 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 2 cut(s) 444, 565
BmrFI CCNGG 2 cut(s) 85, 372
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 5 cut(s) 388, 403, 425, 469, 628
BmuI ACTGGG 1 cut(s) 238
BpuEI CTTGAG 2 cut(s) 134, 564
BpuMI CCSGG 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse1I ACTGG 2 cut(s) 233, 259
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 4 cut(s) 403, 484, 507, 643
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 505
BseNI ACTGG 2 cut(s) 233, 259
BseXI GCAGC 2 cut(s) 541, 653
BshFI GGCC 2 cut(s) 58, 225
BshNI GGYRCC 1 cut(s) 442
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 549
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 4 cut(s) 20, 162, 544, 648
BsmBI CGTCTC 2 cut(s) 20, 648
BsmFI GGGAC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 2 cut(s) 58, 225
Bsp143I GATC 1 cut(s) 603
BspACI CCGC 2 cut(s) 55, 79
BspANI GGCC 2 cut(s) 58, 225
BspCNI CTCAG 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 444, 565
BspT107I GGYRCC 1 cut(s) 442
BsrI ACTGG 2 cut(s) 233, 259
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 389, 491
BstF5I GGATG 4 cut(s) 403, 484, 507, 643
BstKTI GATC 1 cut(s) 606
BstMAI GTCTC 4 cut(s) 20, 162, 544, 648
BstMBI GATC 1 cut(s) 603
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 83, 370
BstV1I GCAGC 2 cut(s) 541, 653
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 2 cut(s) 58, 225
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 4 cut(s) 403, 484, 507, 643
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 563
Csp6I GTAC 1 cut(s) 68
CviAII CATG 1 cut(s) 646
CviJI RGCY 7 cut(s) 58, 74, 225, 370, 578, 614, 644
CviKI_1 RGCY 7 cut(s) 58, 74, 225, 370, 578, 614, 644
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 2 cut(s) 389, 491
DpnI GATC 1 cut(s) 605
DpnII GATC 1 cut(s) 603
DraI TTTAAA 1 cut(s) 474
EaeI YGGCCR 2 cut(s) 56, 223
Eco47I GGWCC 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 370
Esp3I CGTCTC 2 cut(s) 20, 648
FaeI CATG 1 cut(s) 649
FaqI GGGAC 1 cut(s) 549
FatI CATG 1 cut(s) 645
FauI CCCGC 1 cut(s) 86
Fnu4HI GCNGC 3 cut(s) 56, 555, 642
FokI GGATG 4 cut(s) 410, 491, 514, 650
Fsp4HI GCNGC 3 cut(s) 56, 555, 642
GluI GCNGC 3 cut(s) 56, 555, 642
HaeIII GGCC 2 cut(s) 58, 225
HapII CCGG 1 cut(s) 85
Hin1II CATG 1 cut(s) 649
HinfI GANTC 3 cut(s) 211, 248, 489
HpaII CCGG 1 cut(s) 85
HphI GGTGA 2 cut(s) 325, 607
Hpy188I TCNGA 1 cut(s) 412
Hpy188III TCNNGA 3 cut(s) 151, 545, 581
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 403
HpyCH4V TGCA 5 cut(s) 4, 401, 428, 438, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 2 cut(s) 389, 491
HpySE526I ACGT 1 cut(s) 403
Hsp92II CATG 1 cut(s) 649
Kzo9I GATC 1 cut(s) 603
LpnPI CCDG 9 cut(s) 94, 98, 172, 214, 267, 272, 357, 364, 384
Lsp1109I GCAGC 2 cut(s) 541, 653
LweI GCATC 5 cut(s) 388, 403, 425, 469, 628
MaeII ACGT 1 cut(s) 403
MaeIII GTNAC 2 cut(s) 161, 380
MalI GATC 1 cut(s) 605
MboI GATC 1 cut(s) 603
MboII GAAGA 2 cut(s) 610, 613
MfeI CAATTG 1 cut(s) 183
MlsI TGGCCA 1 cut(s) 225
MluCI AATT 8 cut(s) 31, 37, 50, 183, 239, 318, 568, 608
MluNI TGGCCA 1 cut(s) 225
MlyI GAGTC 1 cut(s) 483
MnlI CCTC 4 cut(s) 5, 52, 158, 616
Mox20I TGGCCA 1 cut(s) 225
MscI TGGCCA 1 cut(s) 225
MseI TTAA 3 cut(s) 473, 680, 694
MslI CAYNNNNRTG 1 cut(s) 433
Msp20I TGGCCA 1 cut(s) 225
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 85, 372
MunI CAATTG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 372
NciI CCSGG 1 cut(s) 85
NdeII GATC 1 cut(s) 603
NlaIII CATG 1 cut(s) 649
NlaIV GGNNCC 2 cut(s) 444, 565
NmuCI GTSAC 2 cut(s) 161, 380
PctI GAATGC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 211, 248
PkrI GCNGC 3 cut(s) 57, 556, 643
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 444, 565
PspPI GGNCC 1 cut(s) 563
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 433
SaqAI TTAA 3 cut(s) 473, 680, 694
SatI GCNGC 3 cut(s) 56, 555, 642
Sau3AI GATC 1 cut(s) 603
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 2 cut(s) 85, 372
SetI ASST 3 cut(s) 406, 580, 616
SfaNI GCATC 5 cut(s) 388, 403, 425, 469, 628
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 433
SmlI CTYRAG 2 cut(s) 149, 579
SmoI CTYRAG 2 cut(s) 149, 579
Sse9I AATT 8 cut(s) 31, 37, 50, 183, 239, 318, 568, 608
SsiI CCGC 2 cut(s) 55, 79
StyD4I CCNGG 2 cut(s) 83, 370
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 1 cut(s) 406
TaqI TCGA 3 cut(s) 105, 285, 606
TasI AATT 8 cut(s) 31, 37, 50, 183, 239, 318, 568, 608
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 2 cut(s) 211, 248
Tru1I TTAA 3 cut(s) 473, 680, 694
Tru9I TTAA 3 cut(s) 473, 680, 694
TscAI CASTG 1 cut(s) 233
TseFI GTSAC 2 cut(s) 161, 380
TseI GCWGC 2 cut(s) 554, 641
Tsp45I GTSAC 2 cut(s) 161, 380
TspDTI ATGAA 2 cut(s) 252, 536
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 563
XapI RAATTY 2 cut(s) 37, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.