RchiOBHm_Chr2g0098241

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007911
Physical Location & Seq
Reverse (-)
10654750 .. 10657658
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47304

Sequence Viewer

Length: 984 bp
ATGATGCTCGGAGCTGCCCAATTCTCCGTCGCCGATCATCGCTCGAATGCCGTTATTCCGATCCCAAATACTCGGCCAGCTGTTTCTTCTTCTTCCTCTTCTCCTCACACCTCCGATTTCGTCGTCGTTCGGTTAGGTTTCAGACTCAAACGCCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCAACTCTTCCCCTCACGACGGATCCCTTAATTCCGATCATCAAAACTACGAGTATATCCAAGCTTCCCTCTTGCTTTCAGAAACCATATCTCACTACCGTATGCAAAAGAGAGGATTTCAAGAAGAGACGAATTGGAATTCCTCTGGTAAATTGCGGCCATTTTCGGTCCGAGCCACCCGCCCCCGGAATGTTTTGGACACACTAGACAGGAACTTTCTCCACCGTTTCCAAAGTCCTACCATTTTCCTCAAGATTTCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGATAGAGAATCTCATAAATAGCCACTGGGGAGATGAATTTGGGGATTCCCCAGACCAGTTTCAGTTTGTGAAAAATCTTGTCGAAAAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTGCTGAATACTTACTTTGCCAGATTATATTTTAGCAAGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCACGTCCATCAGATGCCATAAATGTTGCATATAGATGCAAGGCACCCATATATGTAAATAAACAGATTGTTTTAAAAGATGCTGTAAGACTCAGTTATGGGATGGGCAGAATGCGTGATATGAAGTCTACTTACGATGTCTCTCTTGATAGTGCTGCAGATGGTCCCAATTTTTTAGCTCAAGAACTTGTTTTGGTGAAGAAGATCGAATTAGCTATCAAAGAGGAAAGATATAAGGATGCAGCCATGTGGAGAGACGAACTAGTCAAACTTCGCAAGTTAAGTCAAGGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

327

Amino Acids

37.28

Weight (kDa)

9.48

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 152 - 258 6.6e-19 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 950
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 815
AciI CCGC 4 cut(s) 154, 178, 343, 367
AclWI GGATC 4 cut(s) 55, 169, 204, 217
AcoI YGGCCR 2 cut(s) 74, 344
AcsI RAATTY 2 cut(s) 325, 527
AfiI CCNNNNNNNGG 2 cut(s) 206, 372
AgsI TTSAA 1 cut(s) 308
AhlI ACTAGT 1 cut(s) 949
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 5 cut(s) 14, 80, 251, 866, 902
AluI AGCT 5 cut(s) 14, 80, 251, 866, 902
Alw26I GTCTC 4 cut(s) 308, 450, 832, 936
AlwI GGATC 4 cut(s) 55, 169, 204, 217
AoxI GGCC 2 cut(s) 74, 344
ApeKI GCWGC 3 cut(s) 14, 842, 929
ApoI RAATTY 2 cut(s) 325, 527
AspS9I GGNCC 2 cut(s) 355, 851
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 613, 895
AvaII GGWCC 2 cut(s) 355, 851
BamHI GGATCC 1 cut(s) 209
BanI GGYRCC 1 cut(s) 730
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
BbvI GCAGC 2 cut(s) 829, 941
BccI CCATC 4 cut(s) 594, 703, 784, 842
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 660
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 308, 450, 832, 936
BcuI ACTAGT 1 cut(s) 949
BfaI CTAG 2 cut(s) 392, 950
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 5 cut(s) 15, 154, 344, 843, 930
BlsI GCNGC 5 cut(s) 16, 155, 345, 844, 931
Bme1390I CCNGG 2 cut(s) 373, 660
Bme18I GGWCC 2 cut(s) 355, 851
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 2 cut(s) 355, 851
BmiI GGNNCC 3 cut(s) 211, 732, 853
BmrFI CCNGG 2 cut(s) 373, 660
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 5 cut(s) 676, 691, 713, 757, 916
BmuI ACTGGG 1 cut(s) 526
BpuEI CTTGAG 2 cut(s) 422, 852
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 160, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 372
Bse1I ACTGG 2 cut(s) 521, 547
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 160, 371
BseGI GGATG 3 cut(s) 691, 795, 931
BseLI CCNNNNNNNGG 2 cut(s) 206, 372
BseMII CTCAG 1 cut(s) 793
BseNI ACTGG 2 cut(s) 521, 547
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 2 cut(s) 829, 941
BshFI GGCC 2 cut(s) 76, 346
BshNI GGYRCC 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 173
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 2 cut(s) 206, 372
BsmAI GTCTC 4 cut(s) 308, 450, 832, 936
BsmBI CGTCTC 2 cut(s) 308, 936
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 2 cut(s) 52, 804
BsnI GGCC 2 cut(s) 76, 346
Bsp143I GATC 6 cut(s) 34, 60, 174, 209, 223, 891
BspACI CCGC 4 cut(s) 154, 178, 343, 367
BspANI GGCC 2 cut(s) 76, 346
BspCNI CTCAG 1 cut(s) 792
BspDI ATCGAT 1 cut(s) 173
BspLI GGNNCC 3 cut(s) 211, 732, 853
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 4 cut(s) 55, 169, 204, 217
BspT107I GGYRCC 1 cut(s) 730
BsrI ACTGG 2 cut(s) 521, 547
BssECI CCNNGG 2 cut(s) 160, 371
BssMI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 3 cut(s) 161, 287, 413
Bst6I CTCTTC 3 cut(s) 103, 199, 306
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 78, 656
BstDEI CTNAG 2 cut(s) 677, 779
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 3 cut(s) 691, 795, 931
BstKTI GATC 6 cut(s) 37, 63, 177, 212, 226, 894
BstMAI GTCTC 4 cut(s) 308, 450, 832, 936
BstMBI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
BstMWI GCNNNNNNNGC 2 cut(s) 186, 660
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 2 cut(s) 371, 658
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 2 cut(s) 829, 941
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 173
BsuRI GGCC 2 cut(s) 76, 346
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 3 cut(s) 691, 795, 931
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 78, 656
Cfr13I GGNCC 2 cut(s) 355, 851
ClaI ATCGAT 1 cut(s) 173
CpoI CGGWCCG 1 cut(s) 355
CspI CGGWCCG 1 cut(s) 355
CviAII CATG 1 cut(s) 934
DdeI CTNAG 2 cut(s) 677, 779
DpnI GATC 6 cut(s) 36, 62, 176, 211, 225, 893
DpnII GATC 6 cut(s) 34, 60, 174, 209, 223, 891
DraI TTTAAA 1 cut(s) 762
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
EaeI YGGCCR 2 cut(s) 74, 344
Eam1104I CTCTTC 3 cut(s) 103, 199, 306
EarI CTCTTC 3 cut(s) 103, 199, 306
Eco47I GGWCC 2 cut(s) 355, 851
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 658
Esp3I CGTCTC 2 cut(s) 308, 936
FaeI CATG 1 cut(s) 937
FaqI GGGAC 1 cut(s) 837
FatI CATG 1 cut(s) 933
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 1 cut(s) 815
Fnu4HI GCNGC 5 cut(s) 15, 154, 344, 843, 930
FokI GGATG 3 cut(s) 698, 802, 938
Fsp4HI GCNGC 5 cut(s) 15, 154, 344, 843, 930
FspBI CTAG 2 cut(s) 392, 950
GluI GCNGC 5 cut(s) 15, 154, 344, 843, 930
HaeIII GGCC 2 cut(s) 76, 346
HapII CCGG 1 cut(s) 373
Hin1II CATG 1 cut(s) 937
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 5 cut(s) 144, 165, 499, 536, 777
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 613, 895
Hpy166II GTNNAC 2 cut(s) 164, 816
Hpy188I TCNGA 8 cut(s) 11, 60, 115, 143, 223, 268, 359, 700
Hpy188III TCNNGA 5 cut(s) 203, 308, 439, 833, 869
Hpy8I GTNNAC 2 cut(s) 164, 816
Hpy99I CGWCG 4 cut(s) 32, 125, 128, 209
HpyCH4III ACNGT 3 cut(s) 161, 287, 413
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 7 cut(s) 189, 292, 689, 716, 726, 845, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 660
HpyF3I CTNAG 2 cut(s) 677, 779
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 1 cut(s) 937
Kzo9I GATC 6 cut(s) 34, 60, 174, 209, 223, 891
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 2 cut(s) 829, 941
LweI GCATC 5 cut(s) 676, 691, 713, 757, 916
MaeI CTAG 2 cut(s) 392, 950
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 2 cut(s) 449, 668
MalI GATC 6 cut(s) 36, 62, 176, 211, 225, 893
MboI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
MboII GAAGA 8 cut(s) 78, 81, 84, 90, 186, 323, 898, 901
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 209
MlyI GAGTC 3 cut(s) 138, 159, 771
MnlI CCTC 9 cut(s) 106, 114, 121, 210, 266, 293, 340, 446, 904
MseI TTAA 4 cut(s) 216, 761, 968, 982
MslI CAYNNNNRTG 1 cut(s) 721
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 2 cut(s) 373, 660
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 2 cut(s) 52, 804
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 2 cut(s) 186, 660
NciI CCSGG 1 cut(s) 373
NdeII GATC 6 cut(s) 34, 60, 174, 209, 223, 891
NlaIII CATG 1 cut(s) 937
NlaIV GGNNCC 3 cut(s) 211, 732, 853
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 449, 668
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 2 cut(s) 52, 804
PfeI GAWTC 2 cut(s) 499, 536
PkrI GCNGC 5 cut(s) 16, 155, 345, 844, 931
PleI GAGTC 3 cut(s) 138, 159, 771
PpsI GAGTC 3 cut(s) 138, 159, 771
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 211, 732, 853
PspPI GGNCC 2 cut(s) 355, 851
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 721
Rsr2I CGGWCCG 1 cut(s) 355
RsrII CGGWCCG 1 cut(s) 355
SaqAI TTAA 4 cut(s) 216, 761, 968, 982
SatI GCNGC 5 cut(s) 15, 154, 344, 843, 930
Sau3AI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
Sau96I GGNCC 2 cut(s) 355, 851
SchI GAGTC 3 cut(s) 138, 159, 771
ScrFI CCNGG 2 cut(s) 373, 660
SetI ASST 9 cut(s) 16, 82, 113, 139, 188, 253, 694, 868, 904
SfaNI GCATC 5 cut(s) 676, 691, 713, 757, 916
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 2 cut(s) 355, 851
SmiMI CAYNNNNRTG 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 437, 867
SmoI CTYRAG 2 cut(s) 437, 867
SpeI ACTAGT 1 cut(s) 949
SsiI CCGC 4 cut(s) 154, 178, 343, 367
SspMI CTAG 2 cut(s) 392, 950
StyD4I CCNGG 2 cut(s) 371, 658
TaaI ACNGT 3 cut(s) 161, 287, 413
TaiI ACGT 1 cut(s) 694
TaqI TCGA 4 cut(s) 44, 173, 573, 894
TaqII GACCGA 1 cut(s) 343
TauI GCSGC 2 cut(s) 156, 346
TfiI GAWTC 2 cut(s) 499, 536
Tru1I TTAA 4 cut(s) 216, 761, 968, 982
Tru9I TTAA 4 cut(s) 216, 761, 968, 982
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 2 cut(s) 449, 668
TseI GCWGC 3 cut(s) 14, 842, 929
Tsp45I GTSAC 2 cut(s) 449, 668
TspDTI ATGAA 2 cut(s) 540, 824
TspGWI ACGGA 3 cut(s) 16, 222, 468
TspRI CASTG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 355, 851
XapI RAATTY 2 cut(s) 325, 527
XmiI GTMKAC 1 cut(s) 815
XspI CTAG 2 cut(s) 392, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.