Rh2CG123200

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007911
Physical Location & Seq
Reverse (-)
10893174 .. 10897127
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG123200.1

Sequence Viewer

Length: 861 bp
ATGATGCTCGGAGCTGCCCAATTCTCCGTCGCCGATCATCGCTCGAATGCGGTTTTTCCGATCCCAAATACTCGGCCAGCTGTTTCTTCCTCCTCTTCTCCTCACACCTCCGATTTCGTCGTCGTTCGGTTAGGTTTCAGACCCAAACGCCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCAACTCTTCCCCTCACGACAGATCCCTTAATTCCGATCATCAAAACTACGAGTATATCCAAGCTTCCCTCTTGCTTTCAGAAACCATATCTCACTACCGTATGCAAAAGAGAGGATTTCAAGAAGAGACGAATTGGAATTCCTCTGGTAAATTGCGGCCATTTTCGGTCCGAGCCACCCGCCCCCGCCCCCGGAATGTTTTGGATACACTCGACAGGAACTTTCTCCACCGTTTCCAAAGTCCTACCATTTTCCTCAAGATTTCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGGTAGAGAATCTCATAAATAGCCACTGGGGAGATGAATTCGGGGACTCCCCAGACCAGTTTCAGTTTGTGAAAAATCTTGTCGAAAAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTACTGAATACTTACTTTGCCAGATTGTATTTTAGCAAGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCACGTCCATCAGATGCCATAAATGTTGCATATAGATGCAAGGTATGTGTGTCTTTCTCGCCTTCTCAGCACAAGTTGCTCATAAACTTAATGTGCATGCATGTCCTTGCTTTCTCATGTGTAAGTAAGAAACTGTTACCTCCCTGCTGTGTTGAGACTCACAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

286

Amino Acids

32.6

Weight (kDa)

9.5

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 153 - 249 1.9e-14 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 191
AciI CCGC 7 cut(s) 50, 151, 175, 340, 364, 370, 494
AclWI GGATC 3 cut(s) 55, 166, 201
AcoI YGGCCR 2 cut(s) 74, 341
AcsI RAATTY 2 cut(s) 322, 530
AfaI GTAC 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 1 cut(s) 305
AjiI CACGTC 1 cut(s) 695
AjnI CCWGG 1 cut(s) 661
AluBI AGCT 3 cut(s) 14, 80, 248
AluI AGCT 3 cut(s) 14, 80, 248
Alw26I GTCTC 3 cut(s) 305, 453, 839
AlwI GGATC 3 cut(s) 55, 166, 201
AoxI GGCC 2 cut(s) 74, 341
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 2 cut(s) 322, 530
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 352
BccI CCATC 2 cut(s) 597, 706
BciT130I CCWGG 1 cut(s) 663
BciVI GTATCC 1 cut(s) 382
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 3 cut(s) 305, 453, 839
BfuAI ACCTGC 1 cut(s) 191
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 3 cut(s) 15, 151, 341
BlsI GCNGC 3 cut(s) 16, 152, 342
BmcAI AGTACT 1 cut(s) 624
Bme1390I CCNGG 2 cut(s) 376, 663
Bme18I GGWCC 1 cut(s) 352
BmgBI CACGTC 1 cut(s) 695
BmgT120I GGNCC 1 cut(s) 352
BmrFI CCNGG 2 cut(s) 376, 663
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 679, 694, 716
BmuI ACTGGG 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 157, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 2 cut(s) 524, 550
BseBI CCWGG 1 cut(s) 663
BseCI ATCGAT 1 cut(s) 170
BseDI CCNNGG 2 cut(s) 157, 374
BseGI GGATG 1 cut(s) 694
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 2 cut(s) 524, 550
BseRI GAGGAG 2 cut(s) 82, 90
BshFI GGCC 2 cut(s) 76, 343
BshVI ATCGAT 1 cut(s) 170
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 3 cut(s) 305, 453, 839
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 2 cut(s) 76, 343
Bsp143I GATC 5 cut(s) 34, 60, 171, 206, 220
BspACI CCGC 7 cut(s) 50, 151, 175, 340, 364, 370, 494
BspANI GGCC 2 cut(s) 76, 343
BspCNI CTCAG 1 cut(s) 769
BspDI ATCGAT 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 3 cut(s) 55, 166, 201
BsrI ACTGG 2 cut(s) 524, 550
BssECI CCNNGG 2 cut(s) 157, 374
BssMI GATC 5 cut(s) 34, 60, 171, 206, 220
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 4 cut(s) 158, 284, 416, 825
Bst6I CTCTTC 3 cut(s) 100, 196, 303
BstAPI GCANNNNNTGC 2 cut(s) 183, 766
BstC8I GCNNGC 3 cut(s) 78, 659, 788
BstDEI CTNAG 2 cut(s) 680, 756
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 694
BstKTI GATC 5 cut(s) 37, 63, 174, 209, 223
BstMAI GTCTC 3 cut(s) 305, 453, 839
BstMBI GATC 5 cut(s) 34, 60, 171, 206, 220
BstMWI GCNNNNNNNGC 4 cut(s) 183, 663, 757, 766
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 2 cut(s) 790, 794
BstSCI CCNGG 2 cut(s) 374, 661
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
Bsu15I ATCGAT 1 cut(s) 170
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 76, 343
BsuTUI ATCGAT 1 cut(s) 170
BtgI CCRYGG 1 cut(s) 157
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 695
BtsCI GGATG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 3 cut(s) 78, 659, 788
Cfr13I GGNCC 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 170
CpoI CGGWCCG 1 cut(s) 352
Csp6I GTAC 1 cut(s) 623
CspI CGGWCCG 1 cut(s) 352
CviAII CATG 3 cut(s) 787, 791, 807
CviJI RGCY 8 cut(s) 14, 76, 80, 248, 343, 359, 516, 661
CviKI_1 RGCY 8 cut(s) 14, 76, 80, 248, 343, 359, 516, 661
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 2 cut(s) 680, 756
DpnI GATC 5 cut(s) 36, 62, 173, 208, 222
DpnII GATC 5 cut(s) 34, 60, 171, 206, 220
EaeI YGGCCR 2 cut(s) 74, 341
Eam1104I CTCTTC 3 cut(s) 100, 196, 303
EarI CTCTTC 3 cut(s) 100, 196, 303
Eco47I GGWCC 1 cut(s) 352
EcoRI GAATTC 2 cut(s) 322, 530
EcoRII CCWGG 1 cut(s) 661
EcoT22I ATGCAT 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 3 cut(s) 790, 794, 810
FaqI GGGAC 1 cut(s) 551
FatI CATG 3 cut(s) 786, 790, 806
FauI CCCGC 2 cut(s) 371, 377
Fnu4HI GCNGC 3 cut(s) 15, 151, 341
FokI GGATG 1 cut(s) 701
Fsp4HI GCNGC 3 cut(s) 15, 151, 341
GluI GCNGC 3 cut(s) 15, 151, 341
HaeIII GGCC 2 cut(s) 76, 343
HapII CCGG 1 cut(s) 376
Hin1II CATG 3 cut(s) 790, 794, 810
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 4 cut(s) 162, 502, 539, 847
HpaII CCGG 1 cut(s) 376
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 8 cut(s) 11, 60, 112, 140, 220, 265, 356, 703
Hpy188III TCNNGA 3 cut(s) 200, 305, 442
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 3 cut(s) 32, 122, 125
HpyAV CCTTC 1 cut(s) 762
HpyCH4III ACNGT 4 cut(s) 158, 284, 416, 825
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 7 cut(s) 186, 289, 692, 719, 729, 786, 790
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 663, 757, 766
HpyF3I CTNAG 2 cut(s) 680, 756
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 3 cut(s) 790, 794, 810
Kzo9I GATC 5 cut(s) 34, 60, 171, 206, 220
LmnI GCTCC 1 cut(s) 11
LweI GCATC 3 cut(s) 679, 694, 716
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 3 cut(s) 452, 671, 825
MalI GATC 5 cut(s) 36, 62, 173, 208, 222
MboI GATC 5 cut(s) 34, 60, 171, 206, 220
MboII GAAGA 4 cut(s) 78, 87, 183, 320
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 206
MluCI AATT 8 cut(s) 20, 214, 316, 322, 335, 474, 530, 609
MlyI GAGTC 3 cut(s) 156, 533, 841
Mph1103I ATGCAT 1 cut(s) 792
MseI TTAA 3 cut(s) 213, 779, 859
MslI CAYNNNNRTG 1 cut(s) 724
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 376
MspR9I CCNGG 2 cut(s) 376, 663
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 4 cut(s) 183, 663, 757, 766
NciI CCSGG 1 cut(s) 376
NdeII GATC 5 cut(s) 34, 60, 171, 206, 220
NlaIII CATG 3 cut(s) 790, 794, 810
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 452, 671
NsiI ATGCAT 1 cut(s) 792
NspI RCATGY 2 cut(s) 790, 794
PaeI GCATGC 1 cut(s) 790
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 3 cut(s) 16, 152, 342
PleI GAGTC 3 cut(s) 156, 533, 841
PpsI GAGTC 3 cut(s) 156, 533, 841
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspPI GGNCC 1 cut(s) 352
PsuI RGATCY 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
RseI CAYNNNNRTG 1 cut(s) 724
Rsr2I CGGWCCG 1 cut(s) 352
RsrII CGGWCCG 1 cut(s) 352
SaqAI TTAA 3 cut(s) 213, 779, 859
SatI GCNGC 3 cut(s) 15, 151, 341
Sau3AI GATC 5 cut(s) 34, 60, 171, 206, 220
Sau96I GGNCC 1 cut(s) 352
ScaI AGTACT 1 cut(s) 624
SchI GAGTC 3 cut(s) 156, 533, 841
ScrFI CCNGG 2 cut(s) 376, 663
SetI ASST 9 cut(s) 16, 82, 110, 136, 185, 250, 697, 735, 832
SfaNI GCATC 3 cut(s) 679, 694, 716
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 724
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
SphI GCATGC 1 cut(s) 790
Sse9I AATT 8 cut(s) 20, 214, 316, 322, 335, 474, 530, 609
SsiI CCGC 7 cut(s) 50, 151, 175, 340, 364, 370, 494
StyD4I CCNGG 2 cut(s) 374, 661
TaaI ACNGT 4 cut(s) 158, 284, 416, 825
TaiI ACGT 1 cut(s) 697
TaqI TCGA 4 cut(s) 44, 170, 396, 576
TaqII GACCGA 1 cut(s) 340
TasI AATT 8 cut(s) 20, 214, 316, 322, 335, 474, 530, 609
TatI WGTACW 1 cut(s) 622
TauI GCSGC 2 cut(s) 153, 343
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 3 cut(s) 213, 779, 859
Tru9I TTAA 3 cut(s) 213, 779, 859
TscAI CASTG 1 cut(s) 524
TseFI GTSAC 2 cut(s) 452, 671
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 2 cut(s) 452, 671
TspDTI ATGAA 1 cut(s) 543
TspGWI ACGGA 2 cut(s) 16, 471
TspRI CASTG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 2 cut(s) 322, 530
XceI RCATGY 2 cut(s) 790, 794
ZrmI AGTACT 1 cut(s) 624
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.