AT5G66050

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007911
Physical Location & Seq
Reverse (-)
26411796 .. 26414609
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66050.3

Sequence Viewer

Length: 1023 bp
ATGCCCGGAGTTGCCTTCTCCTGTGTTCCTGCCTCCGATCTGGGAATCTTCATCTCCGAATCCACCACCTTCTCACGCTCCATCTCCGCCTCCCTCTCATCTTCCTCCAGTTCACCCTCCTCCTTTCGCTGTTGCACCCCATTGAACCTCAAATCGCTGCGTTCTCATCGCCGTCCTAAGATTTCTCATATTTGTTGCGACGGAAGCTCTATTTCCGGATCTGGCCTCGGCGATCCCGATCTCGAGTTTCTCCAAGCCTCTGTCCTCGTTGCAGAGACAAGTATGCATTACAAAATGCGGCGACATGGTTTCCGGCAAGATTCAATGTGGCAAACTTCTAGGCCTTTGCCTCCCTTTTCTATTAGAGCTAGTGAATCCAGGGTTGGTGTTCTCCCTATTGGACTTGGCTTTCTCCGTCAATTCAAGCAGCCCACTATTTTTCTCAAGATCTCCTGTGATGGTGACTACTTGCTTCCTGTTATTGTTGGGGATGCTGCTGTTGAGAAACTTTTAGACGTACCACTACAAGGCCACACTGAGGAATGCCCTGATCAGTTCCAGTTCGTGTCTGCTGTTGTCGACAAACTTGGATATGAAGTTAAAATGGTGAAACTTACAGGTAGAATTGTAAACACTTATTATGCAAGTCTATGTCTTGGCAAGCCTGGGGACATTGAGGCTATATGCATTGATTCACGGCCATCAGATGCCATCAATGTTGCAAGAGCATGCCAGGCTCCAATATATGTGAACAAAGCAATAGTGCTGGAAGAGGCTATCAAAATTGGTTATGGAGGCAGACCGCAGAGCGCAAAGCCTGTTTTCAATGTTATCCTTGACAGCGCTCCCGATGGACCAGATCCTTTATCGGAAGAGCTTAAATTAGTGAGGAATATGGATTTGGCGTCTAAAGAGGAAAGGTACATCGATGCAGCGATGTGGAGGGACAGACTGAAGAACCTTCAGAACTCGAGCTCTGTTGTATATAATAAGGGTGTAGAAACTCCTGAGAGCTATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

340

Amino Acids

37.22

Weight (kDa)

6.54

Isoelectric Point (pI)

62.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 154 - 261 4.6e-16 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 579
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 3 cut(s) 87, 298, 803
AclWI GGATC 3 cut(s) 226, 227, 854
AcoI YGGCCR 1 cut(s) 698
AcuI CTGAAG 2 cut(s) 947, 974
AcyI GRCGYC 1 cut(s) 905
AfaI GTAC 2 cut(s) 519, 923
AfeI AGCGCT 1 cut(s) 844
AfiI CCNNNNNNNGG 2 cut(s) 527, 538
AgsI TTSAA 4 cut(s) 145, 324, 424, 826
AjnI CCWGG 3 cut(s) 377, 664, 732
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 884, 916
AluBI AGCT 5 cut(s) 207, 368, 877, 975, 1014
AluI AGCT 5 cut(s) 207, 368, 877, 975, 1014
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 226, 227, 854
Ama87I CYCGRG 2 cut(s) 242, 970
Aor13HI TCCGGA 1 cut(s) 215
Aor51HI AGCGCT 1 cut(s) 844
AoxI GGCC 4 cut(s) 223, 341, 529, 698
ApeKI GCWGC 4 cut(s) 157, 427, 494, 932
AspLEI GCGC 2 cut(s) 812, 845
AspS9I GGNCC 1 cut(s) 854
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 3 cut(s) 105, 473, 619
AvaI CYCGRG 2 cut(s) 242, 970
AvaII GGWCC 1 cut(s) 854
BanII GRGCYC 1 cut(s) 977
Bbv12I GWGCWC 1 cut(s) 977
BbvI GCAGC 4 cut(s) 144, 439, 481, 944
BccI CCATC 5 cut(s) 89, 452, 709, 719, 845
BceAI ACGGC 2 cut(s) 156, 713
BciT130I CCWGG 3 cut(s) 379, 666, 734
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 339, 369
BfoI RGCGCY 1 cut(s) 846
BglII AGATCT 1 cut(s) 447
BisI GCNGC 5 cut(s) 158, 299, 428, 495, 933
BlsI GCNGC 5 cut(s) 159, 300, 429, 496, 934
Bme1390I CCNGG 4 cut(s) 6, 379, 666, 734
Bme18I GGWCC 1 cut(s) 854
BmeT110I CYCGRG 2 cut(s) 242, 970
BmgT120I GGNCC 1 cut(s) 854
BmiI GGNNCC 1 cut(s) 738
BmrFI CCNGG 4 cut(s) 6, 379, 666, 734
BmsI GCATC 3 cut(s) 481, 697, 919
BpmI CTGGAG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 927
BsaBI GATNNNNATC 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 226, 378, 665
BsaWI WCCGGW 1 cut(s) 215
Bsc4I CCNNNNNNNGG 2 cut(s) 527, 538
Bse1I ACTGG 2 cut(s) 108, 559
Bse8I GATNNNNATC 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 3 cut(s) 379, 666, 734
BseCI ATCGAT 1 cut(s) 927
BseDI CCNNGG 3 cut(s) 226, 378, 665
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 2 cut(s) 527, 538
BseMII CTCAG 2 cut(s) 528, 999
BseNI ACTGG 2 cut(s) 108, 559
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 4 cut(s) 144, 439, 481, 944
BshFI GGCC 4 cut(s) 225, 343, 531, 700
BshVI ATCGAT 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 977
BsiHKCI CYCGRG 2 cut(s) 242, 970
BsiSI CCGG 3 cut(s) 6, 216, 313
BslFI GGGAC 2 cut(s) 683, 959
BslI CCNNNNNNNGG 2 cut(s) 527, 538
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 683, 959
BsmI GAATGC 1 cut(s) 548
BsnI GGCC 4 cut(s) 225, 343, 531, 700
BsoBI CYCGRG 2 cut(s) 242, 970
Bsp1286I GDGCHC 1 cut(s) 977
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
BspACI CCGC 3 cut(s) 87, 298, 803
BspANI GGCC 4 cut(s) 225, 343, 531, 700
BspCNI CTCAG 2 cut(s) 529, 1000
BspDI ATCGAT 1 cut(s) 927
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 738
BspPI GGATC 3 cut(s) 226, 227, 854
BspQI GCTCTTC 1 cut(s) 867
BsrI ACTGG 2 cut(s) 108, 559
BssECI CCNNGG 3 cut(s) 226, 378, 665
BssMI GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
BssNI GRCGYC 1 cut(s) 905
Bst2UI CCWGG 3 cut(s) 379, 666, 734
Bst6I CTCTTC 2 cut(s) 765, 867
BstACI GRCGYC 1 cut(s) 905
BstC8I GCNNGC 2 cut(s) 662, 730
BstDEI CTNAG 3 cut(s) 177, 537, 1008
BstF5I GGATG 1 cut(s) 496
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 2 cut(s) 812, 845
BstKTI GATC 7 cut(s) 40, 221, 235, 241, 450, 553, 862
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
BstMWI GCNNNNNNNGC 2 cut(s) 204, 734
BstNI CCWGG 3 cut(s) 379, 666, 734
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 4 cut(s) 4, 377, 664, 732
BstV1I GCAGC 4 cut(s) 144, 439, 481, 944
BstX2I RGATCY 3 cut(s) 218, 447, 859
BstYI RGATCY 3 cut(s) 218, 447, 859
Bsu15I ATCGAT 1 cut(s) 927
BsuRI GGCC 4 cut(s) 225, 343, 531, 700
BsuTUI ATCGAT 1 cut(s) 927
BtgZI GCGATG 2 cut(s) 152, 950
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 2 cut(s) 662, 730
CfoI GCGC 2 cut(s) 812, 845
Cfr13I GGNCC 1 cut(s) 854
ClaI ATCGAT 1 cut(s) 927
CseI GACGC 1 cut(s) 894
Csp6I GTAC 2 cut(s) 518, 922
CviAII CATG 2 cut(s) 305, 729
CviQI GTAC 2 cut(s) 518, 922
DdeI CTNAG 3 cut(s) 177, 537, 1008
DpnI GATC 7 cut(s) 39, 220, 234, 240, 449, 552, 861
DpnII GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
EaeI YGGCCR 1 cut(s) 698
Eam1104I CTCTTC 2 cut(s) 765, 867
EarI CTCTTC 2 cut(s) 765, 867
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 975
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 977
Eco47I GGWCC 1 cut(s) 854
Eco47III AGCGCT 1 cut(s) 844
Eco53kI GAGCTC 1 cut(s) 975
Eco57I CTGAAG 2 cut(s) 947, 974
Eco88I CYCGRG 2 cut(s) 242, 970
EcoICRI GAGCTC 1 cut(s) 975
EcoRII CCWGG 3 cut(s) 377, 664, 732
EcoT22I ATGCAT 2 cut(s) 288, 689
EcoT38I GRGCYC 1 cut(s) 977
FaeI CATG 2 cut(s) 308, 732
FaqI GGGAC 2 cut(s) 683, 959
FatI CATG 2 cut(s) 304, 728
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 5 cut(s) 158, 299, 428, 495, 933
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 977
Fsp4HI GCNGC 5 cut(s) 158, 299, 428, 495, 933
FspBI CTAG 2 cut(s) 339, 369
GlaI GCGC 2 cut(s) 811, 844
GluI GCNGC 5 cut(s) 158, 299, 428, 495, 933
GsuI CTGGAG 1 cut(s) 91
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 4 cut(s) 225, 343, 531, 700
HapII CCGG 3 cut(s) 6, 216, 313
HgaI GACGC 1 cut(s) 894
HhaI GCGC 2 cut(s) 812, 845
Hin1I GRCGYC 1 cut(s) 905
Hin1II CATG 2 cut(s) 308, 732
Hin6I GCGC 2 cut(s) 810, 843
HinP1I GCGC 2 cut(s) 810, 843
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 5 cut(s) 45, 59, 320, 374, 692
HpaII CCGG 3 cut(s) 6, 216, 313
HphI GGTGA 3 cut(s) 105, 473, 619
Hpy166II GTNNAC 4 cut(s) 113, 580, 631, 751
Hpy188I TCNGA 5 cut(s) 37, 58, 706, 871, 966
Hpy188III TCNNGA 6 cut(s) 216, 236, 242, 445, 848, 1007
Hpy8I GTNNAC 4 cut(s) 113, 580, 631, 751
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 25, 79, 971
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 7 cut(s) 135, 272, 286, 644, 687, 722, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 204, 734
HpyF3I CTNAG 3 cut(s) 177, 537, 1008
HpySE526I ACGT 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 905
Hsp92II CATG 2 cut(s) 308, 732
HspAI GCGC 2 cut(s) 810, 843
Kpn2I TCCGGA 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 3 cut(s) 83, 742, 850
Lsp1109I GCAGC 4 cut(s) 144, 439, 481, 944
LweI GCATC 3 cut(s) 481, 697, 919
MaeI CTAG 2 cut(s) 339, 369
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 461
MalI GATC 7 cut(s) 39, 220, 234, 240, 449, 552, 861
MboI GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
MboII GAAGA 5 cut(s) 40, 93, 782, 884, 967
MflI RGATCY 3 cut(s) 218, 447, 859
MhlI GDGCHC 1 cut(s) 977
MluCI AATT 4 cut(s) 419, 624, 783, 881
Mph1103I ATGCAT 2 cut(s) 288, 689
MroI TCCGGA 1 cut(s) 215
MseI TTAA 2 cut(s) 600, 879
MspI CCGG 3 cut(s) 6, 216, 313
MspR9I CCNGG 4 cut(s) 6, 379, 666, 734
Mva1269I GAATGC 1 cut(s) 548
MvaI CCWGG 3 cut(s) 379, 666, 734
MwoI GCNNNNNNNGC 2 cut(s) 204, 734
NciI CCSGG 1 cut(s) 6
NdeII GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
NlaIII CATG 2 cut(s) 308, 732
NlaIV GGNNCC 1 cut(s) 738
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 461
NsiI ATGCAT 2 cut(s) 288, 689
NspI RCATGY 1 cut(s) 732
PaeI GCATGC 1 cut(s) 732
PaeR7I CTCGAG 2 cut(s) 242, 970
PceI AGGCCT 1 cut(s) 343
PciSI GCTCTTC 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 1 cut(s) 548
PfeI GAWTC 5 cut(s) 45, 59, 320, 374, 692
PkrI GCNGC 5 cut(s) 159, 300, 429, 496, 934
Psp124BI GAGCTC 1 cut(s) 977
Psp6I CCWGG 3 cut(s) 377, 664, 732
PspGI CCWGG 3 cut(s) 377, 664, 732
PspN4I GGNNCC 1 cut(s) 738
PspPI GGNCC 1 cut(s) 854
PspXI VCTCGAGB 1 cut(s) 970
PsuI RGATCY 3 cut(s) 218, 447, 859
RsaI GTAC 2 cut(s) 519, 923
RsaNI GTAC 2 cut(s) 518, 922
SacI GAGCTC 1 cut(s) 977
SalI GTCGAC 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 867
SaqAI TTAA 2 cut(s) 600, 879
SatI GCNGC 5 cut(s) 158, 299, 428, 495, 933
Sau3AI GATC 7 cut(s) 37, 218, 232, 238, 447, 550, 859
Sau96I GGNCC 1 cut(s) 854
ScrFI CCNGG 4 cut(s) 6, 379, 666, 734
SduI GDGCHC 1 cut(s) 977
SfaNI GCATC 3 cut(s) 481, 697, 919
Sfr274I CTCGAG 2 cut(s) 242, 970
SinI GGWCC 1 cut(s) 854
SlaI CTCGAG 2 cut(s) 242, 970
SmlI CTYRAG 3 cut(s) 242, 443, 970
SmoI CTYRAG 3 cut(s) 242, 443, 970
SphI GCATGC 1 cut(s) 732
Sse9I AATT 4 cut(s) 419, 624, 783, 881
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 3 cut(s) 87, 298, 803
SspMI CTAG 2 cut(s) 339, 369
SstI GAGCTC 1 cut(s) 977
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 4 cut(s) 4, 377, 664, 732
TaiI ACGT 1 cut(s) 519
TaqI TCGA 4 cut(s) 243, 579, 927, 971
TasI AATT 4 cut(s) 419, 624, 783, 881
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 5 cut(s) 45, 59, 320, 374, 692
Tru1I TTAA 2 cut(s) 600, 879
Tru9I TTAA 2 cut(s) 600, 879
TscAI CASTG 1 cut(s) 541
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 4 cut(s) 157, 427, 494, 932
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 2 cut(s) 40, 609
TspGWI ACGGA 2 cut(s) 216, 404
TspRI CASTG 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 854
XceI RCATGY 1 cut(s) 732
XhoI CTCGAG 2 cut(s) 242, 970
XmiI GTMKAC 1 cut(s) 579
XspI CTAG 2 cut(s) 339, 369
Zsp2I ATGCAT 2 cut(s) 288, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.