RLG00000016778

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007911
Physical Location & Seq
Reverse (-)
9903474 .. 9905915
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016778

Sequence Viewer

Length: 999 bp
ATGATGCTCGGAGCTGCCCAATTCTCCGTCGCCGATCATCGCTCGAATGCCGTTTTTCCGATCCCAAATACTCGGCCAGCTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTTCTCCTCACACCTCCGATTTCGTCGTCGTTCGGTTAGGTTTCAGACCCAAACACCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCAACTCTTCCCCTCACGACAGATCCCTTAATTCCGATCATCAAAACTACGAGTATATCCAAGCTTCCCTCTTGCTTTCAGAAACTATATCTCACTACCGTATGCAAAAGAGAGGATTTCAAGAAGAGACGAATTGGAATTCCTCTGGTAAATTGCGGCCATTTTCGGTCCGAGCCACCCGGCCCCGGAATGTTTTGGATACACTAGACAGGAGCTTTCTCCACCGTTTCCAAAGTCCTACCATTTTCCTCAAGATTTCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGATAGAGAATCTCATAAATGGCCACTGGGGAGATGAATTTGGGGATTCCCCAGACCAGTTTCAGTTTGTGAAAAATCTTGTCAAAAAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTGCTGAATACTTACTTTGCCAGATTGTATTTTAGCAAGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCACGTCCATCAGATGCCATAAATGTTGCATATAGATGCAAGGCACCCATATTTGTAAATAAACAGATTGTTTTAAAAGATGCTGTAAGAATCAGTTATGGGATGGGCAGAATGCGTGATATGAAGTCTACTTACGATGTCTCTCTTGATAGTGCTGCAGATGGTCCCAATTTTTTAGCTCAAGAACTTGTTTTGGTGAAGAAGATCGAATTAGCTATCAAAGAGGAAAGATATAAGGATGCAGCCATGTGGAGAGACGAACTAGTCAAACTTCGCAAGTTAAGTCAAGGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

333

Amino Acids

37.73

Weight (kDa)

9.62

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 157 - 264 1.9e-18 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 965
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 830
AciI CCGC 3 cut(s) 169, 193, 358
AclWI GGATC 3 cut(s) 55, 184, 219
AcoI YGGCCR 3 cut(s) 74, 359, 526
AcsI RAATTY 2 cut(s) 340, 542
AfiI CCNNNNNNNGG 1 cut(s) 387
AgsI TTSAA 1 cut(s) 323
AhlI ACTAGT 1 cut(s) 964
AjiI CACGTC 1 cut(s) 707
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 6 cut(s) 14, 80, 266, 417, 881, 917
AluI AGCT 6 cut(s) 14, 80, 266, 417, 881, 917
Alw26I GTCTC 4 cut(s) 323, 465, 847, 951
AlwI GGATC 3 cut(s) 55, 184, 219
AoxI GGCC 4 cut(s) 74, 359, 383, 526
ApeKI GCWGC 3 cut(s) 14, 857, 944
ApoI RAATTY 2 cut(s) 340, 542
AspS9I GGNCC 3 cut(s) 370, 384, 866
AsuC2I CCSGG 2 cut(s) 382, 388
AsuHPI GGTGA 2 cut(s) 628, 910
AvaII GGWCC 2 cut(s) 370, 866
BalI TGGCCA 1 cut(s) 528
BanI GGYRCC 1 cut(s) 745
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
BbvI GCAGC 2 cut(s) 844, 956
BccI CCATC 4 cut(s) 609, 718, 799, 857
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 675
BciVI GTATCC 1 cut(s) 394
BcnI CCSGG 2 cut(s) 382, 388
BcoDI GTCTC 4 cut(s) 323, 465, 847, 951
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 2 cut(s) 407, 965
BfmI CTRYAG 1 cut(s) 858
BfuAI ACCTGC 1 cut(s) 209
BfuI GTATCC 1 cut(s) 394
BisI GCNGC 4 cut(s) 15, 359, 858, 945
BlsI GCNGC 4 cut(s) 16, 360, 859, 946
Bme1390I CCNGG 3 cut(s) 382, 388, 675
Bme18I GGWCC 2 cut(s) 370, 866
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 3 cut(s) 370, 384, 866
BmiI GGNNCC 3 cut(s) 386, 747, 868
BmrFI CCNGG 3 cut(s) 382, 388, 675
BmrI ACTGGG 1 cut(s) 541
BmsI GCATC 5 cut(s) 691, 706, 728, 772, 931
BmuI ACTGGG 1 cut(s) 541
BpuEI CTTGAG 2 cut(s) 437, 867
BpuMI CCSGG 2 cut(s) 382, 388
Bsa29I ATCGAT 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 175, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 387
Bse1I ACTGG 2 cut(s) 536, 562
BseBI CCWGG 1 cut(s) 675
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 175, 386
BseGI GGATG 3 cut(s) 706, 810, 946
BseLI CCNNNNNNNGG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 536, 562
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 844, 956
BshFI GGCC 4 cut(s) 76, 361, 385, 528
BshNI GGYRCC 1 cut(s) 745
BshVI ATCGAT 1 cut(s) 188
BsiSI CCGG 2 cut(s) 382, 388
BslFI GGGAC 1 cut(s) 852
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 4 cut(s) 323, 465, 847, 951
BsmBI CGTCTC 2 cut(s) 323, 951
BsmFI GGGAC 1 cut(s) 852
BsmI GAATGC 2 cut(s) 52, 819
BsnI GGCC 4 cut(s) 76, 361, 385, 528
Bsp143I GATC 6 cut(s) 34, 60, 189, 224, 238, 906
BspACI CCGC 3 cut(s) 169, 193, 358
BspANI GGCC 4 cut(s) 76, 361, 385, 528
BspDI ATCGAT 1 cut(s) 188
BspLI GGNNCC 3 cut(s) 386, 747, 868
BspMAI CTGCAG 1 cut(s) 862
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 3 cut(s) 55, 184, 219
BspT107I GGYRCC 1 cut(s) 745
BsrI ACTGG 2 cut(s) 536, 562
BssECI CCNNGG 2 cut(s) 175, 386
BssMI GATC 6 cut(s) 34, 60, 189, 224, 238, 906
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 3 cut(s) 176, 302, 428
Bst6I CTCTTC 3 cut(s) 118, 214, 321
BstAPI GCANNNNNTGC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 78, 671
BstDEI CTNAG 1 cut(s) 692
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 3 cut(s) 706, 810, 946
BstKTI GATC 6 cut(s) 37, 63, 192, 227, 241, 909
BstMAI GTCTC 4 cut(s) 323, 465, 847, 951
BstMBI GATC 6 cut(s) 34, 60, 189, 224, 238, 906
BstMWI GCNNNNNNNGC 2 cut(s) 201, 675
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 3 cut(s) 380, 386, 673
BstSFI CTRYAG 1 cut(s) 858
BstV1I GCAGC 2 cut(s) 844, 956
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
Bsu15I ATCGAT 1 cut(s) 188
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 4 cut(s) 76, 361, 385, 528
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 3 cut(s) 706, 810, 946
BtsIMutI CAGTG 1 cut(s) 529
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 2 cut(s) 78, 671
Cfr13I GGNCC 3 cut(s) 370, 384, 866
ClaI ATCGAT 1 cut(s) 188
CpoI CGGWCCG 1 cut(s) 370
CspI CGGWCCG 1 cut(s) 370
CviAII CATG 1 cut(s) 949
DdeI CTNAG 1 cut(s) 692
DpnI GATC 6 cut(s) 36, 62, 191, 226, 240, 908
DpnII GATC 6 cut(s) 34, 60, 189, 224, 238, 906
DraI TTTAAA 1 cut(s) 777
DrdI GACNNNNNNGTC 1 cut(s) 965
DseDI GACNNNNNNGTC 1 cut(s) 965
EaeI YGGCCR 3 cut(s) 74, 359, 526
Eam1104I CTCTTC 3 cut(s) 118, 214, 321
EarI CTCTTC 3 cut(s) 118, 214, 321
Eco47I GGWCC 2 cut(s) 370, 866
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 673
Esp3I CGTCTC 2 cut(s) 323, 951
FaeI CATG 1 cut(s) 952
FaqI GGGAC 1 cut(s) 852
FatI CATG 1 cut(s) 948
FblI GTMKAC 1 cut(s) 830
Fnu4HI GCNGC 4 cut(s) 15, 359, 858, 945
FokI GGATG 3 cut(s) 713, 817, 953
Fsp4HI GCNGC 4 cut(s) 15, 359, 858, 945
FspBI CTAG 2 cut(s) 407, 965
GluI GCNGC 4 cut(s) 15, 359, 858, 945
HaeIII GGCC 4 cut(s) 76, 361, 385, 528
HapII CCGG 2 cut(s) 382, 388
Hin1II CATG 1 cut(s) 952
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 4 cut(s) 180, 514, 551, 792
HpaII CCGG 2 cut(s) 382, 388
HphI GGTGA 2 cut(s) 628, 910
Hpy166II GTNNAC 2 cut(s) 179, 831
Hpy188I TCNGA 8 cut(s) 11, 60, 130, 158, 238, 283, 374, 715
Hpy188III TCNNGA 5 cut(s) 218, 323, 454, 848, 884
Hpy8I GTNNAC 2 cut(s) 179, 831
Hpy99I CGWCG 3 cut(s) 32, 140, 143
HpyCH4III ACNGT 3 cut(s) 176, 302, 428
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 7 cut(s) 204, 307, 704, 731, 741, 860, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 675
HpyF3I CTNAG 1 cut(s) 692
HpySE526I ACGT 1 cut(s) 706
Hsp92II CATG 1 cut(s) 952
Kzo9I GATC 6 cut(s) 34, 60, 189, 224, 238, 906
LmnI GCTCC 2 cut(s) 11, 414
Lsp1109I GCAGC 2 cut(s) 844, 956
LweI GCATC 5 cut(s) 691, 706, 728, 772, 931
MaeI CTAG 2 cut(s) 407, 965
MaeII ACGT 1 cut(s) 706
MaeIII GTNAC 2 cut(s) 464, 683
MalI GATC 6 cut(s) 36, 62, 191, 226, 240, 908
MboI GATC 6 cut(s) 34, 60, 189, 224, 238, 906
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 528
MluNI TGGCCA 1 cut(s) 528
MlyI GAGTC 1 cut(s) 174
MnlI CCTC 9 cut(s) 121, 129, 136, 225, 281, 308, 355, 461, 919
Mox20I TGGCCA 1 cut(s) 528
MscI TGGCCA 1 cut(s) 528
MseI TTAA 4 cut(s) 231, 776, 983, 997
MslI CAYNNNNRTG 1 cut(s) 736
Msp20I TGGCCA 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 382, 388
MspR9I CCNGG 3 cut(s) 382, 388, 675
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 2 cut(s) 52, 819
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 2 cut(s) 201, 675
NciI CCSGG 2 cut(s) 382, 388
NdeII GATC 6 cut(s) 34, 60, 189, 224, 238, 906
NlaIII CATG 1 cut(s) 952
NlaIV GGNNCC 3 cut(s) 386, 747, 868
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 464, 683
PctI GAATGC 2 cut(s) 52, 819
PfeI GAWTC 3 cut(s) 514, 551, 792
PkrI GCNGC 4 cut(s) 16, 360, 859, 946
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 3 cut(s) 386, 747, 868
PspPI GGNCC 3 cut(s) 370, 384, 866
PstI CTGCAG 1 cut(s) 862
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 736
Rsr2I CGGWCCG 1 cut(s) 370
RsrII CGGWCCG 1 cut(s) 370
SaqAI TTAA 4 cut(s) 231, 776, 983, 997
SatI GCNGC 4 cut(s) 15, 359, 858, 945
Sau3AI GATC 6 cut(s) 34, 60, 189, 224, 238, 906
Sau96I GGNCC 3 cut(s) 370, 384, 866
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 3 cut(s) 382, 388, 675
SfaNI GCATC 5 cut(s) 691, 706, 728, 772, 931
SfcI CTRYAG 1 cut(s) 858
SinI GGWCC 2 cut(s) 370, 866
SmiMI CAYNNNNRTG 1 cut(s) 736
SmlI CTYRAG 2 cut(s) 452, 882
SmoI CTYRAG 2 cut(s) 452, 882
SpeI ACTAGT 1 cut(s) 964
SsiI CCGC 3 cut(s) 169, 193, 358
SspMI CTAG 2 cut(s) 407, 965
StyD4I CCNGG 3 cut(s) 380, 386, 673
TaaI ACNGT 3 cut(s) 176, 302, 428
TaiI ACGT 1 cut(s) 709
TaqI TCGA 3 cut(s) 44, 188, 909
TaqII GACCGA 1 cut(s) 358
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 3 cut(s) 514, 551, 792
Tru1I TTAA 4 cut(s) 231, 776, 983, 997
Tru9I TTAA 4 cut(s) 231, 776, 983, 997
TscAI CASTG 1 cut(s) 536
TseFI GTSAC 2 cut(s) 464, 683
TseI GCWGC 3 cut(s) 14, 857, 944
Tsp45I GTSAC 2 cut(s) 464, 683
TspDTI ATGAA 2 cut(s) 555, 839
TspGWI ACGGA 2 cut(s) 16, 483
TspRI CASTG 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 370, 866
XapI RAATTY 2 cut(s) 340, 542
XmiI GTMKAC 1 cut(s) 830
XspI CTAG 2 cut(s) 407, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.