MD02G1122800.v1.1

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007911
Physical Location & Seq
Reverse (-)
9985037 .. 9987714
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1122800.v1.1.491

Sequence Viewer

Length: 978 bp
ATGCTCGGAGCCAGAGCTCAATGCCACGGCCCGGATCGCTTAGCTGCCATCCGTCCGATTCCAAACACTTCTTCTTCTTCCTCCTCCTCTTCGCGCCACTTGGTCTGCACGGTTCGGTTAGGGTTTATCAGATCACCAAAACGGTGCCGTTCGCTCAAATCGCTCCGCATTACCTGCTGCAACTCTTCCTCTCGCTCCAGATCCAGCAATGCCGACGATCATCACGATTTCGACTACATTCAAGCTTCCCTCCTTCTCTCTGAAACTATATCTCACTACCGTATGCAAAGGCGAGGATTTCAAGAAGAGACGAAATGGCAGTCATCTGGTAAAATGCGTCCCTTTTCCGTCCGAGCCAGCCGACAACATCATTTTTTCGATACGTTAGGCCGAAACTTTGTCCAGCGTTTCCAAAGTCCGACGATTTTTCTGAAGATTTCTTGTGATGGAGACTTTTTGCTTCCAATTGTTGTAGGTGAATTCGCGATCGAAAATCTCATAGACGGCCAATGGGGAGATGAGAATGGGGACTCCCCGGATCAATTTCACTTTATGAGAAATCTCGTTGACAAACTCGGCTATCAAGTGAATATGGTGAGAATTACGGAAAGAGTGCGGAATACTTACTTTGCCAGATTGTACTTAAGCAAGCCAGGGGAAAATGGCTTTCTTAGTGTTGAAGCACGCCCGTCAGATGCCATAAATTTCGCACATAGATGCAAGGCACCCATATATGTAAACAAACAGATTGTCTTAACAGATGCGGTGAGGATCAGTTATGGGATGGGCGGAGGGCGCGAGACAAAGTCTTGTTATGACGTATCTCTTGACAGTGCTATAGAGGGCCCAGATTCTTTAATTCAAGAACTTGGTTTGGTCAAGAATATCAATCAAGCTATCAAAGAGGAAAGATATAAAGATGCAGCCATATGGAGAGACGAACTATTCAAGCTTCGCAAGTCAATTCAAGGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

326

Amino Acids

36.92

Weight (kDa)

9.35

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 150 - 256 5.8e-19 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 182
AccB1I GGYRCC 2 cut(s) 144, 724
AccII CGCG 3 cut(s) 94, 485, 798
AciI CCGC 4 cut(s) 166, 616, 764, 789
AclWI GGATC 4 cut(s) 42, 195, 546, 779
AcoI YGGCCR 1 cut(s) 505
AcsI RAATTY 2 cut(s) 479, 703
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 1 cut(s) 31
AflII CTTAAG 1 cut(s) 643
AgsI TTSAA 6 cut(s) 242, 302, 680, 863, 949, 968
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 5 cut(s) 17, 44, 245, 896, 952
AluI AGCT 5 cut(s) 17, 44, 245, 896, 952
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 4 cut(s) 302, 444, 794, 930
AlwI GGATC 4 cut(s) 42, 195, 546, 779
AoxI GGCC 5 cut(s) 28, 388, 505, 844, 971
ApaI GGGCCC 1 cut(s) 848
ApeKI GCWGC 3 cut(s) 44, 177, 923
ApoI RAATTY 2 cut(s) 479, 703
ArsI GACNNNNNNTTYG 4 cut(s) 305, 337, 735, 767
AspLEI GCGC 2 cut(s) 96, 798
AspS9I GGNCC 4 cut(s) 29, 844, 845, 971
AsuC2I CCSGG 2 cut(s) 32, 536
AsuHPI GGTGA 4 cut(s) 126, 488, 607, 778
BaeGI GKGCMC 1 cut(s) 848
BanI GGYRCC 2 cut(s) 144, 724
BanII GRGCYC 2 cut(s) 19, 848
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 31, 164, 935
BccI CCATC 3 cut(s) 56, 440, 778
BceAI ACGGC 3 cut(s) 43, 132, 520
BciT130I CCWGG 1 cut(s) 654
BcnI CCSGG 2 cut(s) 32, 536
BcoDI GTCTC 4 cut(s) 302, 444, 794, 930
BfmI CTRYAG 1 cut(s) 837
BfrI CTTAAG 1 cut(s) 643
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 3 cut(s) 45, 178, 924
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 3 cut(s) 46, 179, 925
Bme1390I CCNGG 3 cut(s) 32, 536, 654
BmgT120I GGNCC 4 cut(s) 29, 844, 845, 971
BmiI GGNNCC 4 cut(s) 10, 146, 726, 846
BmrFI CCNGG 3 cut(s) 32, 536, 654
BmsI GCATC 4 cut(s) 685, 707, 751, 910
BpmI CTGGAG 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 40
BpuMI CCSGG 2 cut(s) 32, 536
BsaJI CCNNGG 3 cut(s) 25, 534, 653
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 3 cut(s) 25, 534, 653
BseGI GGATG 2 cut(s) 48, 789
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 214
BseRI GAGGAG 2 cut(s) 73, 76
BseSI GKGCMC 1 cut(s) 848
BseXI GCAGC 3 cut(s) 31, 164, 935
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 3 cut(s) 94, 485, 798
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 5 cut(s) 30, 390, 507, 846, 973
BshNI GGYRCC 2 cut(s) 144, 724
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 32, 536
BslFI GGGAC 2 cut(s) 324, 542
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 4 cut(s) 302, 444, 794, 930
BsmBI CGTCTC 2 cut(s) 302, 930
BsmFI GGGAC 2 cut(s) 324, 542
BsnI GGCC 5 cut(s) 30, 390, 507, 846, 973
Bsp120I GGGCCC 1 cut(s) 844
Bsp1286I GDGCHC 2 cut(s) 19, 848
Bsp143I GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
Bsp1720I GCTNAGC 1 cut(s) 40
Bsp68I TCGCGA 1 cut(s) 485
BspACI CCGC 4 cut(s) 166, 616, 764, 789
BspANI GGCC 5 cut(s) 30, 390, 507, 846, 973
BspFNI CGCG 3 cut(s) 94, 485, 798
BspLI GGNNCC 4 cut(s) 10, 146, 726, 846
BspMI ACCTGC 1 cut(s) 182
BspPI GGATC 4 cut(s) 42, 195, 546, 779
BspT107I GGYRCC 2 cut(s) 144, 724
BspTI CTTAAG 1 cut(s) 643
BsrDI GCAATG 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 25, 534, 653
BssMI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 4 cut(s) 112, 144, 281, 833
Bst6I CTCTTC 3 cut(s) 94, 190, 300
BstAFI CTTAAG 1 cut(s) 643
BstAPI GCANNNNNTGC 1 cut(s) 174
BstC8I GCNNGC 3 cut(s) 358, 650, 685
BstDEI CTNAG 2 cut(s) 40, 671
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 2 cut(s) 48, 789
BstFNI CGCG 3 cut(s) 94, 485, 798
BstHHI GCGC 2 cut(s) 96, 798
BstKTI GATC 7 cut(s) 37, 134, 203, 220, 489, 541, 774
BstMAI GTCTC 4 cut(s) 302, 444, 794, 930
BstMBI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 3 cut(s) 30, 534, 652
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 3 cut(s) 94, 485, 798
BstV1I GCAGC 3 cut(s) 31, 164, 935
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BsuRI GGCC 5 cut(s) 30, 390, 507, 846, 973
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 48, 789
BtsIMutI CAGTG 1 cut(s) 838
BtuMI TCGCGA 1 cut(s) 485
BveI ACCTGC 1 cut(s) 182
Cac8I GCNNGC 3 cut(s) 358, 650, 685
CfoI GCGC 2 cut(s) 96, 798
Cfr13I GGNCC 4 cut(s) 29, 844, 845, 971
CseI GACGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 640
CviAII CATG 1 cut(s) 975
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 2 cut(s) 40, 671
DpnI GATC 7 cut(s) 36, 133, 202, 219, 488, 540, 773
DpnII GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 3 cut(s) 94, 190, 300
EarI CTCTTC 3 cut(s) 94, 190, 300
EciI GGCGGA 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 2 cut(s) 19, 848
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 652
EcoT38I GRGCYC 2 cut(s) 19, 848
Esp3I CGTCTC 2 cut(s) 302, 930
FaeI CATG 1 cut(s) 978
FaqI GGGAC 2 cut(s) 324, 542
FatI CATG 1 cut(s) 974
FauNDI CATATG 1 cut(s) 929
Fnu4HI GCNGC 3 cut(s) 45, 178, 924
FokI GGATG 2 cut(s) 35, 796
FriOI GRGCYC 2 cut(s) 19, 848
Fsp4HI GCNGC 3 cut(s) 45, 178, 924
GlaI GCGC 2 cut(s) 95, 797
GluI GCNGC 3 cut(s) 45, 178, 924
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 5 cut(s) 30, 390, 507, 846, 973
HapII CCGG 2 cut(s) 32, 536
HgaI GACGC 1 cut(s) 326
HhaI GCGC 2 cut(s) 96, 798
Hin1II CATG 1 cut(s) 978
Hin6I GCGC 2 cut(s) 94, 796
HinP1I GCGC 2 cut(s) 94, 796
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 243, 950
HinfI GANTC 3 cut(s) 58, 530, 851
HpaII CCGG 2 cut(s) 32, 536
HphI GGTGA 4 cut(s) 126, 488, 607, 778
Hpy166II GTNNAC 2 cut(s) 568, 739
Hpy188I TCNGA 8 cut(s) 8, 57, 131, 262, 353, 420, 432, 694
Hpy188III TCNNGA 7 cut(s) 198, 224, 302, 484, 827, 863, 880
Hpy8I GTNNAC 2 cut(s) 568, 739
Hpy99I CGWCG 2 cut(s) 218, 424
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 4 cut(s) 112, 144, 281, 833
HpyCH4IV ACGT 2 cut(s) 383, 819
HpyCH4V TGCA 5 cut(s) 108, 180, 286, 720, 923
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
HpyF3I CTNAG 2 cut(s) 40, 671
HpySE526I ACGT 2 cut(s) 383, 819
Hsp92II CATG 1 cut(s) 978
HspAI GCGC 2 cut(s) 94, 796
Kzo9I GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
LmnI GCTCC 3 cut(s) 8, 168, 200
Lsp1109I GCAGC 3 cut(s) 31, 164, 935
LweI GCATC 4 cut(s) 685, 707, 751, 910
MaeII ACGT 2 cut(s) 383, 819
MalI GATC 7 cut(s) 36, 133, 202, 219, 488, 540, 773
MboI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
MboII GAAGA 7 cut(s) 63, 66, 69, 81, 177, 317, 445
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 200
MhlI GDGCHC 2 cut(s) 19, 848
MluCI AATT 7 cut(s) 465, 479, 542, 600, 703, 858, 963
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 443
MseI TTAA 3 cut(s) 644, 755, 857
MslI CAYNNNNRTG 1 cut(s) 715
MspCI CTTAAG 1 cut(s) 643
MspI CCGG 2 cut(s) 32, 536
MspR9I CCNGG 3 cut(s) 32, 536, 654
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 3 cut(s) 94, 485, 798
MwoI GCNNNNNNNGC 4 cut(s) 36, 160, 174, 795
NciI CCSGG 2 cut(s) 32, 536
NdeI CATATG 1 cut(s) 929
NdeII GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
NlaIII CATG 1 cut(s) 978
NlaIV GGNNCC 4 cut(s) 10, 146, 726, 846
NmeAIII GCCGAG 1 cut(s) 555
NruI TCGCGA 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 2 cut(s) 58, 851
PflFI GACNNNGTC 1 cut(s) 805
PkrI GCNGC 3 cut(s) 46, 179, 925
Ple19I CGATCG 1 cut(s) 489
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 4 cut(s) 10, 146, 726, 846
PspOMI GGGCCC 1 cut(s) 844
PspPI GGNCC 4 cut(s) 29, 844, 845, 971
PsuI RGATCY 1 cut(s) 200
PsyI GACNNNGTC 1 cut(s) 805
PvuI CGATCG 1 cut(s) 489
RruI TCGCGA 1 cut(s) 485
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
RseI CAYNNNNRTG 1 cut(s) 715
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 3 cut(s) 644, 755, 857
SatI GCNGC 3 cut(s) 45, 178, 924
Sau3AI GATC 7 cut(s) 34, 131, 200, 217, 486, 538, 771
Sau96I GGNCC 4 cut(s) 29, 844, 845, 971
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 3 cut(s) 32, 536, 654
SduI GDGCHC 2 cut(s) 19, 848
SetI ASST 9 cut(s) 19, 46, 176, 247, 386, 478, 822, 898, 954
SfaNI GCATC 4 cut(s) 685, 707, 751, 910
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 715
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 7 cut(s) 465, 479, 542, 600, 703, 858, 963
SsiI CCGC 4 cut(s) 166, 616, 764, 789
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 3 cut(s) 30, 534, 652
TaaI ACNGT 4 cut(s) 112, 144, 281, 833
TaiI ACGT 2 cut(s) 386, 822
TaqI TCGA 3 cut(s) 231, 378, 489
TasI AATT 7 cut(s) 465, 479, 542, 600, 703, 858, 963
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 2 cut(s) 58, 851
Tru1I TTAA 3 cut(s) 644, 755, 857
Tru9I TTAA 3 cut(s) 644, 755, 857
TscAI CASTG 1 cut(s) 838
TseI GCWGC 3 cut(s) 44, 177, 923
TspGWI ACGGA 3 cut(s) 41, 337, 620
TspRI CASTG 1 cut(s) 838
Tth111I GACNNNGTC 1 cut(s) 805
Vha464I CTTAAG 1 cut(s) 643
XapI RAATTY 2 cut(s) 479, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.