MD15G1236800.v1.1

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007911
Physical Location & Seq
Reverse (-)
19489266 .. 19492012
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1236800.v1.1.491

Sequence Viewer

Length: 975 bp
ATGCTCGGAGCCGGAGCTCATTTCCACGTGACGGATCGCTCAGCTGCCATCCGTCCGGTCCCAAAAACTTCGCCTTCTTCTTCCTCTTCGCGCCGCTTGGTCTTCACGGTTCGATTAGGGTTCATCAGACCATCAAAACGGTGTCGTGCGCTCAAATCTCTCCGCATTACCTGCAACAGCTCTTCCTCTCGCTCTAGATCCAGCAATGCCGACGATCATCACGATTTCGACCACATCCAAGCTTCCCTCCTCCTCTCAGAAACTATATCGCACTACCGTATGCAGAGGCGAGGGTTTCAAGAAGAATCGAAATGGCAGTTATCTGGTAAATTGCGTCCCTTTTCGGTCCACGCCAGCCGACAACAAAACATTCTTGATACATTAGGCCGAAACTTTGTCCAGCGTTTCCAAACTCCCACGATTTTTCTTAAGATTTCTTGTGATGGAGACTTTTTGCTGCCAATTGTTGTAGGCGAATTTGCAATACAAAATCTCATAGATGCCCAGTGGGGAGATGAGAATGGGGACTCCCCTGATCATTTCCAGTTCTTTAGAAACCTTGTTGACAAACTCGGCTATCAAGTGAATATGGTGAGAATTACGGAAAGAGTGCGGAATACTTACTTTGCCAGATTGTACTTTAGCAAGCCAGGGGAAAATGGCTTTCTTAGTGTAGATGCACGCCCGTCAGATGCCATAAATTTCGCACATAGATGCAAGGCACCCATATATGTAAACAAACAGATTGTCTTAACAGATGCAGTGAGGATCAGTTATGGGATGGGCAGAGTGCGCGATACAAAGTCTTGTTACGACGTATCTCTTGACAGTGCTATAGAGGGCCCAGATTCTTTAATTCAAGAACTTTGTTTGGTTAAGAATATCAATTTAGCTATCAAAGAGGAAAGATATAAAGATGCAGCCATGTGGAAAGACGAACTATTCAAGCTTCGCAAGTCAATTCAAGGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

325

Amino Acids

36.91

Weight (kDa)

9.6

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 149 - 255 2.6e-20 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 721
AccII CGCG 2 cut(s) 91, 795
AciI CCGC 3 cut(s) 94, 163, 613
AclWI GGATC 3 cut(s) 42, 192, 776
AcsI RAATTY 2 cut(s) 476, 700
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 1 cut(s) 31
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 4 cut(s) 299, 860, 946, 965
AjnI CCWGG 1 cut(s) 649
AluBI AGCT 6 cut(s) 17, 44, 180, 242, 893, 949
AluI AGCT 6 cut(s) 17, 44, 180, 242, 893, 949
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 441
AlwI GGATC 3 cut(s) 42, 192, 776
AoxI GGCC 3 cut(s) 385, 841, 968
ApaI GGGCCC 1 cut(s) 845
ApeKI GCWGC 3 cut(s) 44, 457, 920
ApoI RAATTY 2 cut(s) 476, 700
ArsI GACNNNNNNTTYG 2 cut(s) 732, 764
AspLEI GCGC 3 cut(s) 93, 151, 795
AspS9I GGNCC 5 cut(s) 58, 346, 841, 842, 968
AsuHPI GGTGA 1 cut(s) 604
AvaII GGWCC 2 cut(s) 58, 346
BaeGI GKGCMC 1 cut(s) 845
BanI GGYRCC 1 cut(s) 721
BanII GRGCYC 2 cut(s) 19, 845
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 31, 444, 932
BccI CCATC 4 cut(s) 56, 139, 437, 775
BciT130I CCWGG 1 cut(s) 651
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 834
BfrI CTTAAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 179
BisI GCNGC 4 cut(s) 45, 94, 458, 921
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 4 cut(s) 46, 95, 459, 922
Bme1390I CCNGG 1 cut(s) 651
Bme18I GGWCC 2 cut(s) 58, 346
BmgT120I GGNCC 5 cut(s) 58, 346, 841, 842, 968
BmiI GGNNCC 4 cut(s) 10, 60, 723, 843
BmrFI CCNGG 1 cut(s) 651
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 6 cut(s) 490, 667, 682, 704, 748, 907
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 1 cut(s) 94
Bpu1102I GCTNAGC 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 650
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 505, 544
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 650
BseGI GGATG 3 cut(s) 48, 234, 786
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 54, 270
BseNI ACTGG 2 cut(s) 505, 544
BseRI GAGGAG 2 cut(s) 239, 242
BseSI GKGCMC 1 cut(s) 845
BseXI GCAGC 3 cut(s) 31, 444, 932
Bsh1236I CGCG 2 cut(s) 91, 795
BshFI GGCC 3 cut(s) 387, 843, 970
BshNI GGYRCC 1 cut(s) 721
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 12, 56
BslFI GGGAC 3 cut(s) 44, 321, 539
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 441
BsmFI GGGAC 3 cut(s) 44, 321, 539
BsnI GGCC 3 cut(s) 387, 843, 970
Bsp120I GGGCCC 1 cut(s) 841
Bsp1286I GDGCHC 2 cut(s) 19, 845
Bsp143I GATC 5 cut(s) 34, 197, 214, 535, 768
Bsp1720I GCTNAGC 1 cut(s) 40
BspACI CCGC 3 cut(s) 94, 163, 613
BspANI GGCC 3 cut(s) 387, 843, 970
BspCNI CTCAG 2 cut(s) 53, 269
BspFNI CGCG 2 cut(s) 91, 795
BspLI GGNNCC 4 cut(s) 10, 60, 723, 843
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 3 cut(s) 42, 192, 776
BspQI GCTCTTC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 721
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 2 cut(s) 505, 544
BssECI CCNNGG 1 cut(s) 650
BssMI GATC 5 cut(s) 34, 197, 214, 535, 768
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 4 cut(s) 109, 141, 278, 830
Bst6I CTCTTC 2 cut(s) 91, 187
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 171
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 355, 647, 682
BstDEI CTNAG 3 cut(s) 40, 256, 668
BstF5I GGATG 3 cut(s) 48, 234, 786
BstFNI CGCG 2 cut(s) 91, 795
BstHHI GCGC 3 cut(s) 93, 151, 795
BstKTI GATC 5 cut(s) 37, 200, 217, 538, 771
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 5 cut(s) 34, 197, 214, 535, 768
BstMWI GCNNNNNNNGC 2 cut(s) 171, 792
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 1 cut(s) 649
BstSFI CTRYAG 1 cut(s) 834
BstSLI GKGCMC 1 cut(s) 845
BstUI CGCG 2 cut(s) 91, 795
BstV1I GCAGC 3 cut(s) 31, 444, 932
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 3 cut(s) 387, 843, 970
BtsCI GGATG 3 cut(s) 48, 234, 786
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 3 cut(s) 512, 768, 835
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 355, 647, 682
CfoI GCGC 3 cut(s) 93, 151, 795
Cfr13I GGNCC 5 cut(s) 58, 346, 841, 842, 968
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 637
CviAII CATG 1 cut(s) 925
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 40, 256, 668
DpnI GATC 5 cut(s) 36, 199, 216, 537, 770
DpnII GATC 5 cut(s) 34, 197, 214, 535, 768
Eam1104I CTCTTC 2 cut(s) 91, 187
EarI CTCTTC 2 cut(s) 91, 187
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 2 cut(s) 19, 845
Eco47I GGWCC 2 cut(s) 58, 346
Eco53kI GAGCTC 1 cut(s) 17
Eco72I CACGTG 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 2 cut(s) 841, 968
EcoRII CCWGG 1 cut(s) 649
EcoT38I GRGCYC 2 cut(s) 19, 845
FaeI CATG 1 cut(s) 928
FaqI GGGAC 3 cut(s) 44, 321, 539
FatI CATG 1 cut(s) 924
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 4 cut(s) 45, 94, 458, 921
FokI GGATG 3 cut(s) 35, 221, 793
FriOI GRGCYC 2 cut(s) 19, 845
Fsp4HI GCNGC 4 cut(s) 45, 94, 458, 921
FspBI CTAG 1 cut(s) 195
GlaI GCGC 3 cut(s) 92, 150, 794
GluI GCNGC 4 cut(s) 45, 94, 458, 921
HaeIII GGCC 3 cut(s) 387, 843, 970
HapII CCGG 2 cut(s) 12, 56
HgaI GACGC 1 cut(s) 323
HhaI GCGC 3 cut(s) 93, 151, 795
Hin1II CATG 1 cut(s) 928
Hin6I GCGC 3 cut(s) 91, 149, 793
HinP1I GCGC 3 cut(s) 91, 149, 793
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 2 cut(s) 240, 947
HinfI GANTC 3 cut(s) 305, 527, 848
HpaII CCGG 2 cut(s) 12, 56
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 3 cut(s) 349, 565, 736
Hpy188I TCNGA 4 cut(s) 8, 128, 259, 691
Hpy188III TCNNGA 6 cut(s) 195, 221, 299, 374, 824, 860
Hpy8I GTNNAC 3 cut(s) 349, 565, 736
Hpy99I CGWCG 2 cut(s) 215, 818
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 4 cut(s) 109, 141, 278, 830
HpyCH4IV ACGT 2 cut(s) 27, 816
HpyCH4V TGCA 7 cut(s) 174, 283, 482, 680, 717, 761, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 792
HpyF3I CTNAG 3 cut(s) 40, 256, 668
HpySE526I ACGT 2 cut(s) 27, 816
Hsp92II CATG 1 cut(s) 928
HspAI GCGC 3 cut(s) 91, 149, 793
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 5 cut(s) 34, 197, 214, 535, 768
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 2 cut(s) 8, 14
Lsp1109I GCAGC 3 cut(s) 31, 444, 932
LweI GCATC 6 cut(s) 490, 667, 682, 704, 748, 907
MaeI CTAG 1 cut(s) 195
MaeII ACGT 2 cut(s) 27, 816
MaeIII GTNAC 2 cut(s) 28, 809
MalI GATC 5 cut(s) 36, 199, 216, 537, 770
MboI GATC 5 cut(s) 34, 197, 214, 535, 768
MboII GAAGA 6 cut(s) 69, 72, 78, 94, 174, 314
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 19, 845
MluCI AATT 8 cut(s) 329, 462, 476, 597, 700, 855, 886, 960
MlyI GAGTC 1 cut(s) 521
MseI TTAA 5 cut(s) 429, 752, 854, 876, 973
MslI CAYNNNNRTG 1 cut(s) 712
MspA1I CMGCKG 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 12, 56
MspR9I CCNGG 1 cut(s) 651
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 91, 795
MwoI GCNNNNNNNGC 2 cut(s) 171, 792
NdeII GATC 5 cut(s) 34, 197, 214, 535, 768
NlaIII CATG 1 cut(s) 928
NlaIV GGNNCC 4 cut(s) 10, 60, 723, 843
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 2 cut(s) 305, 848
PkrI GCNGC 4 cut(s) 46, 95, 459, 922
PleI GAGTC 1 cut(s) 521
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 28
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 649
PspCI CACGTG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 10, 60, 723, 843
PspOMI GGGCCC 1 cut(s) 841
PspPI GGNCC 5 cut(s) 58, 346, 841, 842, 968
PsuI RGATCY 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 712
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 5 cut(s) 429, 752, 854, 876, 973
SatI GCNGC 4 cut(s) 45, 94, 458, 921
Sau3AI GATC 5 cut(s) 34, 197, 214, 535, 768
Sau96I GGNCC 5 cut(s) 58, 346, 841, 842, 968
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 651
SduI GDGCHC 2 cut(s) 19, 845
SfaNI GCATC 6 cut(s) 490, 667, 682, 704, 748, 907
SfcI CTRYAG 1 cut(s) 834
SinI GGWCC 2 cut(s) 58, 346
SmiMI CAYNNNNRTG 1 cut(s) 712
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 8 cut(s) 329, 462, 476, 597, 700, 855, 886, 960
SsiI CCGC 3 cut(s) 94, 163, 613
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 649
TaaI ACNGT 4 cut(s) 109, 141, 278, 830
TaiI ACGT 2 cut(s) 30, 819
TaqI TCGA 3 cut(s) 112, 228, 308
TaqII GACCGA 1 cut(s) 334
TasI AATT 8 cut(s) 329, 462, 476, 597, 700, 855, 886, 960
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 2 cut(s) 305, 848
Tru1I TTAA 5 cut(s) 429, 752, 854, 876, 973
Tru9I TTAA 5 cut(s) 429, 752, 854, 876, 973
TscAI CASTG 3 cut(s) 512, 768, 835
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 44, 457, 920
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 3 cut(s) 41, 47, 617
TspRI CASTG 3 cut(s) 512, 768, 835
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 2 cut(s) 58, 346
XapI RAATTY 2 cut(s) 476, 700
XbaI TCTAGA 1 cut(s) 194
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.