Rorug02G0071500

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007911
Physical Location & Seq
Reverse (-)
5581632 .. 5582286
655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0071500.1

Sequence Viewer

Length: 444 bp
ATGGGCATGCTCTTGAAACTTGTGAATGCAAATTGGACGATTGAAATCAGAGTTTTTACTGGCTACTCGCTTCTCCTCACTGCCCTTAGGATTCCTGATGATGGCAAGACTACAGCCATAGGCATCATCCGAAAAACATACGAAATAGGCTTGCCAATCATCAAGGAAGCCAGTATGGAACACAAAATTGGCTTTATCGAGTCCTCAGCTCTCCCTATTCTCGACAAACTCTTAGAAAATGTAGCGCAGGAAGGGAGTTTTGACTTTGCTTTTGTTGATGCTGACATGAACAACAATTGGAATTACCACAAAAGGCTAATTAAACTGATCAAGATTGGTGGAATAATTATCTATGATAACACACTTTGGGGAGGGACAGTTGCTTTACCTGAAGAAGATGTTCCTGAGACCAAAAGGGAATGGAGGCTATATATGTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

147

Amino Acids

16.72

Weight (kDa)

5.41

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_3 PF01596 2 - 139 2.1e-47 O-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 2 cut(s) 16, 44
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 401
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 1 cut(s) 247
AxyI CCTNAGG 1 cut(s) 86
BbvCI CCTCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 95
BclI TGATCA 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 111
BmsI GCATC 2 cut(s) 132, 268
Bpu10I CCTNAGC 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse1I ACTGG 2 cut(s) 64, 171
Bse21I CCTNAGG 1 cut(s) 86
Bse8I GATNNNNATC 1 cut(s) 44
BseGI GGATG 1 cut(s) 126
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 2 cut(s) 219, 396
BseNI ACTGG 2 cut(s) 64, 171
BseRI GAGGAG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 31
Bso31I GGTCTC 1 cut(s) 401
Bsp143I GATC 1 cut(s) 327
BspCNI CTCAG 2 cut(s) 218, 397
BspTNI GGTCTC 1 cut(s) 401
BsrI ACTGG 2 cut(s) 64, 171
BssMI GATC 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 8, 152
BstDEI CTNAG 4 cut(s) 86, 205, 232, 405
BstF5I GGATG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 1 cut(s) 330
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 111
Bsu36I CCTNAGG 1 cut(s) 86
BtsCI GGATG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 8, 152
CfoI GCGC 1 cut(s) 247
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 2 cut(s) 7, 286
CviJI RGCY 8 cut(s) 63, 116, 150, 170, 192, 209, 316, 427
CviKI_1 RGCY 8 cut(s) 63, 116, 150, 170, 192, 209, 316, 427
DdeI CTNAG 4 cut(s) 86, 205, 232, 405
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 86
FaeI CATG 2 cut(s) 10, 289
FaiI YATR 9 cut(s) 8, 119, 139, 176, 287, 354, 430, 432, 434
FaqI GGGAC 1 cut(s) 388
FatI CATG 2 cut(s) 6, 285
FbaI TGATCA 1 cut(s) 327
FokI GGATG 1 cut(s) 113
GlaI GCGC 1 cut(s) 246
HhaI GCGC 1 cut(s) 247
Hin1II CATG 2 cut(s) 10, 289
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 2 cut(s) 91, 200
Hpy188I TCNGA 2 cut(s) 50, 131
Hpy188III TCNNGA 5 cut(s) 13, 95, 221, 331, 404
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4V TGCA 2 cut(s) 29, 438
HpyF3I CTNAG 4 cut(s) 86, 205, 232, 405
Hsp92II CATG 2 cut(s) 10, 289
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 1 cut(s) 327
LpnPI CCDG 6 cut(s) 45, 108, 184, 233, 402, 417
LweI GCATC 2 cut(s) 132, 268
MalI GATC 1 cut(s) 329
MboI GATC 1 cut(s) 327
MboII GAAGA 2 cut(s) 404, 407
MfeI CAATTG 2 cut(s) 295, 439
MluCI AATT 7 cut(s) 31, 186, 295, 301, 318, 345, 439
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 4 cut(s) 86, 214, 365, 417
MroXI GAANNNNTTC 1 cut(s) 399
MseI TTAA 1 cut(s) 321
MunI CAATTG 2 cut(s) 295, 439
Mva1269I GAATGC 1 cut(s) 31
NdeII GATC 1 cut(s) 327
NlaIII CATG 2 cut(s) 10, 289
NspI RCATGY 1 cut(s) 10
PaeI GCATGC 1 cut(s) 10
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 91
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
SaqAI TTAA 1 cut(s) 321
Sau3AI GATC 1 cut(s) 327
SchI GAGTC 1 cut(s) 209
SetI ASST 2 cut(s) 211, 391
SfaNI GCATC 2 cut(s) 132, 268
SfcI CTRYAG 1 cut(s) 111
SphI GCATGC 1 cut(s) 10
Sse9I AATT 7 cut(s) 31, 186, 295, 301, 318, 345, 439
TaaI ACNGT 1 cut(s) 379
TaqI TCGA 2 cut(s) 198, 222
TasI AATT 7 cut(s) 31, 186, 295, 301, 318, 345, 439
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 1 cut(s) 85
XceI RCATGY 1 cut(s) 10
XmnI GAANNNNTTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.