Rw2G009300

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007911
Physical Location & Seq
Reverse (-)
9334579 .. 9337159
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009300.1

Sequence Viewer

Length: 1014 bp
ATGATGCTCGGAGCTGCCCAATTCTCCGTCGCCGATCATCGCTCGAATGCCCTTTTTCCGATCCCAAATACTCGGCCAGCTGTTTCTTCCTCCTCCTCTTCTCCTCACACCTCCGATTTCGTCGTCGTTCGGTTAGGTTTCAGACCCAAACGCCGCCACCGTGGACTCAAATCGATCCGCATTACCTGCAACTCTTCCCCTGACGACAGATCCCTCAATTCCGATCATCAAAACTACGAGTATATCCAAGCTTCCCTCTTGCTTTCAGAAACCATATCTCACTACCGTATGCAAAAGAGAGGATTTCAACAAGAGACGAATTGGAATTCCTCTGGTAAATTGCGGCCATTTTCGGTCCGAGCCACCCGCCCCCGGAATGTTTTGGATACACTCGACAGGAACTTTCTCCACCGTTTCCAAAGTCCTACCATTTTCCTCAAGATTTCGTGTGACGGAGACTTTTTGCTTCCAATTGTTGTAGGCGAACTTGCGATAGAGAATCTCATAAATGGCCACTGGGGAGATGAATTTGGGGATTCCCCAGACCAGTTTCAGTTTGTGAAAAATCTTGTCGAAAAACTTGACCATCAAGTGAATATGGTGAGAATTACAGAAAGAGTGCTGAATACTTACTTTGCCAGATTGTATTTTAGCAAGCCAGGCAAAAGTGACATTCTTAGTGTGGATGCACGTCCATCAGATGCCATAAATGTTGCATATAGATGCAAGGCACCCATATATGTAAATAAACAGATTGTTTTAAAAGATGCTGTAAGACTCAGTTATGGGATGGGCAGAATGCGTGATATGAAGTCTACTTACGATGTCTCTCTTGATAGTGCTGCAGATGGTCCCAATTTTTTAGCTCAAGAACTTGTTTTGGTGAAGAAGATCGAATTAGCTATCAAAGAGGAAAGATATAAGGATGCAGCCCTGTGGAGAGACGAACTAATCAAACTTCGCAAGTTAATGTACAGTTCCGTAGTATATAGACCCCCAAGTATTTATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

337

Amino Acids

38.61

Weight (kDa)

9.56

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BFN_dom PF02577 152 - 258 1.1e-18 Bifunctional nuclease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 815
AciI CCGC 4 cut(s) 154, 178, 343, 367
AclWI GGATC 3 cut(s) 55, 169, 204
AcoI YGGCCR 3 cut(s) 74, 344, 511
AcsI RAATTY 2 cut(s) 325, 527
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 1 cut(s) 372
AgsI TTSAA 1 cut(s) 308
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 5 cut(s) 14, 80, 251, 866, 902
AluI AGCT 5 cut(s) 14, 80, 251, 866, 902
Alw26I GTCTC 4 cut(s) 308, 450, 832, 936
AlwI GGATC 3 cut(s) 55, 169, 204
AoxI GGCC 3 cut(s) 74, 344, 511
ApeKI GCWGC 3 cut(s) 14, 842, 929
ApoI RAATTY 2 cut(s) 325, 527
AspS9I GGNCC 2 cut(s) 355, 851
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 613, 895
AvaII GGWCC 2 cut(s) 355, 851
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 1 cut(s) 730
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
BbvI GCAGC 2 cut(s) 829, 941
BccI CCATC 4 cut(s) 594, 703, 784, 842
BciT130I CCWGG 1 cut(s) 660
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 308, 450, 832, 936
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 5 cut(s) 15, 154, 344, 843, 930
BlsI GCNGC 5 cut(s) 16, 155, 345, 844, 931
Bme1390I CCNGG 2 cut(s) 373, 660
Bme18I GGWCC 2 cut(s) 355, 851
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 2 cut(s) 355, 851
BmiI GGNNCC 2 cut(s) 732, 853
BmrFI CCNGG 2 cut(s) 373, 660
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 5 cut(s) 676, 691, 713, 757, 916
BmuI ACTGGG 1 cut(s) 526
BpuEI CTTGAG 2 cut(s) 422, 852
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 160, 371
Bsc4I CCNNNNNNNGG 1 cut(s) 372
Bse1I ACTGG 2 cut(s) 521, 547
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 160, 371
BseGI GGATG 3 cut(s) 691, 795, 931
BseLI CCNNNNNNNGG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 793
BseNI ACTGG 2 cut(s) 521, 547
BseRI GAGGAG 3 cut(s) 82, 85, 93
BseXI GCAGC 2 cut(s) 829, 941
BshFI GGCC 3 cut(s) 76, 346, 513
BshNI GGYRCC 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 173
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 4 cut(s) 308, 450, 832, 936
BsmBI CGTCTC 2 cut(s) 308, 936
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 2 cut(s) 52, 804
BsnI GGCC 3 cut(s) 76, 346, 513
Bsp1407I TGTACA 1 cut(s) 972
Bsp143I GATC 6 cut(s) 34, 60, 174, 209, 223, 891
BspACI CCGC 4 cut(s) 154, 178, 343, 367
BspANI GGCC 3 cut(s) 76, 346, 513
BspCNI CTCAG 1 cut(s) 792
BspDI ATCGAT 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 732, 853
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 3 cut(s) 55, 169, 204
BspT107I GGYRCC 1 cut(s) 730
BsrGI TGTACA 1 cut(s) 972
BsrI ACTGG 2 cut(s) 521, 547
BssECI CCNNGG 2 cut(s) 160, 371
BssMI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 4 cut(s) 161, 287, 413, 977
Bst6I CTCTTC 2 cut(s) 103, 199
BstAPI GCANNNNNTGC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 78, 656
BstDEI CTNAG 2 cut(s) 677, 779
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 3 cut(s) 691, 795, 931
BstKTI GATC 6 cut(s) 37, 63, 177, 212, 226, 894
BstMAI GTCTC 4 cut(s) 308, 450, 832, 936
BstMBI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
BstMWI GCNNNNNNNGC 2 cut(s) 186, 660
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 2 cut(s) 371, 658
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 2 cut(s) 829, 941
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 173
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 3 cut(s) 76, 346, 513
BsuTUI ATCGAT 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 3 cut(s) 691, 795, 931
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 78, 656
Cfr13I GGNCC 2 cut(s) 355, 851
ClaI ATCGAT 1 cut(s) 173
CpoI CGGWCCG 1 cut(s) 355
Csp6I GTAC 1 cut(s) 973
CspI CGGWCCG 1 cut(s) 355
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 2 cut(s) 677, 779
DpnI GATC 6 cut(s) 36, 62, 176, 211, 225, 893
DpnII GATC 6 cut(s) 34, 60, 174, 209, 223, 891
DraI TTTAAA 1 cut(s) 762
EaeI YGGCCR 3 cut(s) 74, 344, 511
Eam1104I CTCTTC 2 cut(s) 103, 199
EarI CTCTTC 2 cut(s) 103, 199
Eco47I GGWCC 2 cut(s) 355, 851
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 658
Esp3I CGTCTC 2 cut(s) 308, 936
FaqI GGGAC 1 cut(s) 837
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 1 cut(s) 815
Fnu4HI GCNGC 5 cut(s) 15, 154, 344, 843, 930
FokI GGATG 3 cut(s) 698, 802, 938
Fsp4HI GCNGC 5 cut(s) 15, 154, 344, 843, 930
GluI GCNGC 5 cut(s) 15, 154, 344, 843, 930
HaeIII GGCC 3 cut(s) 76, 346, 513
HapII CCGG 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 4 cut(s) 165, 499, 536, 777
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 613, 895
Hpy166II GTNNAC 2 cut(s) 164, 816
Hpy188I TCNGA 8 cut(s) 11, 60, 115, 143, 223, 268, 359, 700
Hpy188III TCNNGA 3 cut(s) 439, 833, 869
Hpy8I GTNNAC 2 cut(s) 164, 816
Hpy99I CGWCG 3 cut(s) 32, 125, 128
HpyCH4III ACNGT 4 cut(s) 161, 287, 413, 977
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 7 cut(s) 189, 292, 689, 716, 726, 845, 929
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 660
HpyF3I CTNAG 2 cut(s) 677, 779
HpySE526I ACGT 1 cut(s) 691
Kzo9I GATC 6 cut(s) 34, 60, 174, 209, 223, 891
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 2 cut(s) 829, 941
LweI GCATC 5 cut(s) 676, 691, 713, 757, 916
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 2 cut(s) 449, 668
MalI GATC 6 cut(s) 36, 62, 176, 211, 225, 893
MboI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
MboII GAAGA 5 cut(s) 78, 90, 186, 898, 901
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 2 cut(s) 159, 771
Mox20I TGGCCA 1 cut(s) 513
MscI TGGCCA 1 cut(s) 513
MseI TTAA 2 cut(s) 761, 968
MslI CAYNNNNRTG 1 cut(s) 721
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 2 cut(s) 373, 660
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 2 cut(s) 52, 804
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 2 cut(s) 186, 660
NciI CCSGG 1 cut(s) 373
NdeII GATC 6 cut(s) 34, 60, 174, 209, 223, 891
NlaIV GGNNCC 2 cut(s) 732, 853
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 449, 668
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 2 cut(s) 52, 804
PfeI GAWTC 2 cut(s) 499, 536
PkrI GCNGC 5 cut(s) 16, 155, 345, 844, 931
PleI GAGTC 2 cut(s) 159, 771
PpsI GAGTC 2 cut(s) 159, 771
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 2 cut(s) 732, 853
PspPI GGNCC 2 cut(s) 355, 851
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 1 cut(s) 721
Rsr2I CGGWCCG 1 cut(s) 355
RsrII CGGWCCG 1 cut(s) 355
SaqAI TTAA 2 cut(s) 761, 968
SatI GCNGC 5 cut(s) 15, 154, 344, 843, 930
Sau3AI GATC 6 cut(s) 34, 60, 174, 209, 223, 891
Sau96I GGNCC 2 cut(s) 355, 851
SchI GAGTC 2 cut(s) 159, 771
ScrFI CCNGG 2 cut(s) 373, 660
SetI ASST 9 cut(s) 16, 82, 113, 139, 188, 253, 694, 868, 904
SfaNI GCATC 5 cut(s) 676, 691, 713, 757, 916
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 2 cut(s) 355, 851
SmiMI CAYNNNNRTG 1 cut(s) 721
SmlI CTYRAG 2 cut(s) 437, 867
SmoI CTYRAG 2 cut(s) 437, 867
SsiI CCGC 4 cut(s) 154, 178, 343, 367
StyD4I CCNGG 2 cut(s) 371, 658
TaaI ACNGT 4 cut(s) 161, 287, 413, 977
TaiI ACGT 1 cut(s) 694
TaqI TCGA 5 cut(s) 44, 173, 393, 573, 894
TaqII GACCGA 1 cut(s) 343
TatI WGTACW 1 cut(s) 972
TauI GCSGC 2 cut(s) 156, 346
TfiI GAWTC 2 cut(s) 499, 536
Tru1I TTAA 2 cut(s) 761, 968
Tru9I TTAA 2 cut(s) 761, 968
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 2 cut(s) 449, 668
TseI GCWGC 3 cut(s) 14, 842, 929
Tsp45I GTSAC 2 cut(s) 449, 668
TspDTI ATGAA 2 cut(s) 540, 824
TspGWI ACGGA 3 cut(s) 16, 468, 970
TspRI CASTG 1 cut(s) 521
VpaK11BI GGWCC 2 cut(s) 355, 851
XapI RAATTY 2 cut(s) 325, 527
XmiI GTMKAC 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.