Rroxscaffold_2G00144140

ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015516
Physical Location & Seq
Reverse (-)
82112120 .. 82114008
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144140.1

Sequence Viewer

Length: 1110 bp
ATGAATTACACCGATACCCTATTATCTCCTCTCCCAATAAATTATCAATCTGACGATTCGTCACCAGTATGGCTCAACAAGGCAGACAATGCATGGCAACTCACAGCAACCACCATGGTAGCCATGCAAAGTGTTCCGGGGCTCGTGATACTCTATGGTTCCATGGTCAAGAAGAAATGGGCGGTGAACTCCGCGTTCATGGCCCTATACGCCTTTGCCGCGGTGCTCTTGTGTTGGGTGCTTTGGGCTTATAACATGTCATTTGGTATTAGGCTCACCCCATTTGTGGGAAGGCCTGGTTTTGCCATGACCCCAAATATCCTGCACCAATTTGCAGGCTATTATGTCCCAAAGGCTGACTATGTGTACTTTCAATTTGTTTTTGCTGCTATTACTGTGATATTGCTAGCTGGGTCTTTGCTTGGGAGGATGAACTTCTATGCTTGGATGTTGTTTGTACCAATGTGGCTCACACTGTCTTACTCAGTTGGGGCTTTTAGTATTTGGGCAAGTGCTGGGTTTCTTGAGAAACACATCATTGATTATGCTGGCGGATTTGTGATTCATCTTTCTTCTGGTATAGCTGGTTTTATTGCTGCTTATTGGGTAGGACCAAGGCAATCACATGATAGGCAACACTTTCCACCAAACAACATAATTCACATGTTGGGAGGTGCAGGGTTTCTATGGCTTGGTTGGACAGGCTTCAATGGTGGCTCTCCATTTGCAGCAAATGAGATTGCGTCGTTGGCCATTCTCAACACCCATGTCTGCACTGCTACCAGCCTTTTGGTTTGGGTTTCCCTTGATATGATTGTGTACAAGAAAAGCTCTGTGATTGGCGCGGGGAATCCACGTAAAGGTCTTAAACAAATGGGGTACCAGATCCTCGGGGCTCTTTTCATTATCGTATGGAATGTTGTTGTGACGAGCTTGATTTGTATTTTCATCAGCAAAATCGTTGATCTGAGGATGAAGGAAGAAGATCTTGACATTGGTGATGATGCGGTGCATGGAGAAGAAGCCTATGCTATTTGGGGTGATGGTGAGAGTACGTCAGCATACAGGAGGCATGTAAGGATTCCTTCCATCTGTAGACGGCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

369

Amino Acids

40.97

Weight (kDa)

8.75

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 31 - 282 1.8e-44 Ammonium Transporter Family
Ammonium_transp PF00909 288 - 343 9.5e-07 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015516)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10970
malus_domestica MD02G1122700.v1.1
prunus_persica Prupe.7G175800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0098231
rosa_laevigata RLG00000016777
rosa_multiflora Rmu_co8345397.1_g000001 Rmu_sc0009178.1_g000002 Rmu_sc0009922.1_g000007
rosa_roxburghii Rroxscaffold_2G00144140
rosa_rugosa Rorug02G0071300 Rorug02G0071400
rosa_samantha Rh2AG118400 Rh2BG121500 Rh2CG123100 Rh2DG123200
rosa_wichuraiana Rw2G009290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 879
AccB1I GGYRCC 1 cut(s) 879
AccI GTMKAC 1 cut(s) 1096
AccII CGCG 3 cut(s) 194, 221, 845
AciI CCGC 7 cut(s) 182, 192, 219, 221, 552, 845, 1007
AclWI GGATC 1 cut(s) 880
AcoI YGGCCR 1 cut(s) 750
AfaI GTAC 5 cut(s) 368, 459, 821, 881, 1054
AfiI CCNNNNNNNGG 3 cut(s) 286, 287, 860
AflIII ACRYGT 2 cut(s) 255, 663
AgsI TTSAA 2 cut(s) 374, 709
AhdI GACNNNNNGTC 1 cut(s) 58
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 4 cut(s) 410, 584, 831, 933
AluI AGCT 4 cut(s) 410, 584, 831, 933
Alw21I GWGCWC 1 cut(s) 228
AlwI GGATC 1 cut(s) 880
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 3 cut(s) 201, 293, 750
ApeKI GCWGC 3 cut(s) 386, 596, 728
Asp718I GGTACC 1 cut(s) 879
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 202, 611
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 6 cut(s) 54, 196, 268, 1010, 1052, 1058
AsuNHI GCTAGC 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 752
BanI GGYRCC 1 cut(s) 879
BanII GRGCYC 2 cut(s) 144, 898
BauI CACGAG 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 373, 583, 740
BccI CCATC 2 cut(s) 1037, 1097
BciT130I CCWGG 1 cut(s) 297
BcnI CCSGG 1 cut(s) 138
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 1093
BglII AGATCT 1 cut(s) 985
BisI GCNGC 4 cut(s) 219, 387, 597, 729
BlsI GCNGC 4 cut(s) 220, 388, 598, 730
Bme1390I CCNGG 2 cut(s) 138, 297
Bme18I GGWCC 1 cut(s) 611
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 890
BmgT120I GGNCC 2 cut(s) 202, 611
BmiI GGNNCC 2 cut(s) 160, 881
BmrFI CCNGG 2 cut(s) 138, 297
BmsI GCATC 1 cut(s) 994
BmtI GCTAGC 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 138
BsaAI YACGTR 1 cut(s) 857
BsaJI CCNNGG 6 cut(s) 114, 137, 162, 219, 614, 889
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 287, 860
Bse1I ACTGG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 6 cut(s) 114, 137, 162, 219, 614, 889
BseGI GGATG 3 cut(s) 435, 453, 978
BseLI CCNNNNNNNGG 3 cut(s) 286, 287, 860
BseMII CTCAG 2 cut(s) 498, 959
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 3 cut(s) 373, 583, 740
BseYI CCCAGC 2 cut(s) 410, 515
BsgI GTGCAG 3 cut(s) 308, 696, 757
Bsh1236I CGCG 3 cut(s) 194, 221, 845
BshFI GGCC 3 cut(s) 203, 295, 752
BshNI GGYRCC 1 cut(s) 879
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 3 cut(s) 286, 287, 860
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 203, 295, 752
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 3 cut(s) 144, 228, 898
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 3 cut(s) 885, 964, 985
Bsp19I CCATGG 2 cut(s) 114, 162
BspACI CCGC 7 cut(s) 182, 192, 219, 221, 552, 845, 1007
BspANI GGCC 3 cut(s) 203, 295, 752
BspCNI CTCAG 2 cut(s) 497, 960
BspFNI CGCG 3 cut(s) 194, 221, 845
BspLI GGNNCC 2 cut(s) 160, 881
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 880
BspT107I GGYRCC 1 cut(s) 879
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 6 cut(s) 114, 137, 162, 219, 614, 889
BssMI GATC 3 cut(s) 885, 964, 985
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 3 cut(s) 114, 162, 614
Bst2BI CACGAG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 397, 477
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 819
BstBAI YACGTR 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 337, 408, 550
BstDEI CTNAG 2 cut(s) 484, 968
BstDSI CCRYGG 3 cut(s) 114, 162, 219
BstF5I GGATG 3 cut(s) 435, 453, 978
BstFNI CGCG 3 cut(s) 194, 221, 845
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 3 cut(s) 888, 967, 988
BstMBI GATC 3 cut(s) 885, 964, 985
BstMWI GCNNNNNNNGC 5 cut(s) 89, 200, 209, 218, 749
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 3 cut(s) 259, 667, 1076
BstSCI CCNGG 2 cut(s) 136, 295
BstSFI CTRYAG 1 cut(s) 1093
BstUI CGCG 3 cut(s) 194, 221, 845
BstV1I GCAGC 3 cut(s) 373, 583, 740
BstX2I RGATCY 2 cut(s) 885, 985
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 2 cut(s) 885, 985
BsuRI GGCC 3 cut(s) 203, 295, 752
BtgI CCRYGG 3 cut(s) 114, 162, 219
BtsCI GGATG 3 cut(s) 435, 453, 978
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 2 cut(s) 473, 774
Cac8I GCNNGC 3 cut(s) 337, 408, 550
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 202, 611
Cfr42I CCGCGG 1 cut(s) 222
CseI GACGC 1 cut(s) 732
Csp6I GTAC 5 cut(s) 367, 458, 820, 880, 1053
CviQI GTAC 5 cut(s) 367, 458, 820, 880, 1053
DdeI CTNAG 2 cut(s) 484, 968
DpnI GATC 3 cut(s) 887, 966, 987
DpnII GATC 3 cut(s) 885, 964, 985
DriI GACNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 750
Eam1105I GACNNNNNGTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 3 cut(s) 114, 162, 614
Eco147I AGGCCT 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 144, 898
Eco47I GGWCC 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 890
EcoRII CCWGG 1 cut(s) 295
EcoT14I CCWWGG 3 cut(s) 114, 162, 614
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 2 cut(s) 144, 898
ErhI CCWWGG 3 cut(s) 114, 162, 614
FalI AAGNNNNNCTT 4 cut(s) 972, 1004, 1069, 1101
FaqI GGGAC 1 cut(s) 332
FauI CCCGC 1 cut(s) 838
FblI GTMKAC 1 cut(s) 1096
Fnu4HI GCNGC 4 cut(s) 219, 387, 597, 729
FokI GGATG 3 cut(s) 442, 460, 985
FriOI GRGCYC 2 cut(s) 144, 898
Fsp4HI GCNGC 4 cut(s) 219, 387, 597, 729
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 844
GluI GCNGC 4 cut(s) 219, 387, 597, 729
GsaI CCCAGC 2 cut(s) 414, 519
HaeIII GGCC 3 cut(s) 203, 295, 752
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 732
HhaI GCGC 1 cut(s) 845
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HinfI GANTC 4 cut(s) 56, 562, 850, 1081
HpaII CCGG 1 cut(s) 137
HphI GGTGA 6 cut(s) 54, 196, 268, 1010, 1052, 1058
Hpy166II GTNNAC 4 cut(s) 187, 367, 820, 1097
Hpy188I TCNGA 2 cut(s) 52, 969
Hpy188III TCNNGA 4 cut(s) 145, 169, 524, 989
Hpy8I GTNNAC 4 cut(s) 187, 367, 820, 1097
Hpy99I CGWCG 1 cut(s) 748
HpyAV CCTTC 3 cut(s) 285, 970, 1095
HpyCH4III ACNGT 2 cut(s) 397, 477
HpyCH4IV ACGT 2 cut(s) 856, 1055
HpyCH4V TGCA 8 cut(s) 92, 127, 325, 335, 677, 728, 774, 1012
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 200, 209, 218, 749
HpyF3I CTNAG 2 cut(s) 484, 968
HpySE526I ACGT 2 cut(s) 856, 1055
HspAI GCGC 1 cut(s) 843
KpnI GGTACC 1 cut(s) 883
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 3 cut(s) 885, 964, 985
Lsp1109I GCAGC 3 cut(s) 373, 583, 740
LweI GCATC 1 cut(s) 994
MaeI CTAG 1 cut(s) 407
MaeII ACGT 2 cut(s) 856, 1055
MaeIII GTNAC 2 cut(s) 60, 925
MalI GATC 3 cut(s) 887, 966, 987
MboI GATC 3 cut(s) 885, 964, 985
MboII GAAGA 5 cut(s) 184, 564, 992, 995, 1031
MflI RGATCY 2 cut(s) 885, 985
MhlI GDGCHC 3 cut(s) 144, 228, 898
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 5 cut(s) 4, 40, 329, 374, 657
MluNI TGGCCA 1 cut(s) 752
MmeI TCCRAC 1 cut(s) 677
MnlI CCTC 6 cut(s) 39, 420, 665, 899, 963, 1062
Mox20I TGGCCA 1 cut(s) 752
Mph1103I ATGCAT 1 cut(s) 94
MscI TGGCCA 1 cut(s) 752
MseI TTAA 1 cut(s) 867
MslI CAYNNNNRTG 1 cut(s) 67
Msp20I TGGCCA 1 cut(s) 752
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 138, 297
MvaI CCWGG 1 cut(s) 297
MvnI CGCG 3 cut(s) 194, 221, 845
MwoI GCNNNNNNNGC 5 cut(s) 89, 200, 209, 218, 749
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 2 cut(s) 114, 162
NdeII GATC 3 cut(s) 885, 964, 985
NheI GCTAGC 1 cut(s) 406
NlaIV GGNNCC 2 cut(s) 160, 881
NmuCI GTSAC 2 cut(s) 60, 925
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 3 cut(s) 259, 667, 1076
PceI AGGCCT 1 cut(s) 295
PciI ACATGT 2 cut(s) 255, 663
PfeI GAWTC 4 cut(s) 56, 562, 850, 1081
PkrI GCNGC 4 cut(s) 220, 388, 598, 730
Ppu21I YACGTR 1 cut(s) 857
PscI ACATGT 2 cut(s) 255, 663
PsiI TTATAA 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 295
PspFI CCCAGC 2 cut(s) 410, 515
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 160, 881
PspPI GGNCC 2 cut(s) 202, 611
PsuI RGATCY 2 cut(s) 885, 985
RsaI GTAC 5 cut(s) 368, 459, 821, 881, 1054
RsaNI GTAC 5 cut(s) 367, 458, 820, 880, 1053
RseI CAYNNNNRTG 1 cut(s) 67
SacII CCGCGG 1 cut(s) 222
SaqAI TTAA 1 cut(s) 867
SatI GCNGC 4 cut(s) 219, 387, 597, 729
Sau3AI GATC 3 cut(s) 885, 964, 985
Sau96I GGNCC 2 cut(s) 202, 611
ScrFI CCNGG 2 cut(s) 138, 297
SduI GDGCHC 3 cut(s) 144, 228, 898
SetI ASST 8 cut(s) 412, 586, 676, 833, 859, 865, 935, 1058
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 1093
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 1 cut(s) 611
SmiMI CAYNNNNRTG 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 5 cut(s) 4, 40, 329, 374, 657
SseBI AGGCCT 1 cut(s) 295
SsiI CCGC 7 cut(s) 182, 192, 219, 221, 552, 845, 1007
SspMI CTAG 1 cut(s) 407
StuI AGGCCT 1 cut(s) 295
StyD4I CCNGG 2 cut(s) 136, 295
StyI CCWWGG 3 cut(s) 114, 162, 614
TaaI ACNGT 2 cut(s) 397, 477
TaiI ACGT 2 cut(s) 859, 1058
TasI AATT 5 cut(s) 4, 40, 329, 374, 657
TatI WGTACW 2 cut(s) 366, 819
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 4 cut(s) 56, 562, 850, 1081
Tru1I TTAA 1 cut(s) 867
Tru9I TTAA 1 cut(s) 867
TscAI CASTG 2 cut(s) 480, 781
TseFI GTSAC 2 cut(s) 60, 925
TseI GCWGC 3 cut(s) 386, 596, 728
Tsp45I GTSAC 2 cut(s) 60, 925
TspDTI ATGAA 7 cut(s) 17, 187, 446, 554, 892, 937, 989
TspRI CASTG 2 cut(s) 480, 781
VpaK11BI GGWCC 1 cut(s) 611
XceI RCATGY 3 cut(s) 259, 667, 1076
XmiI GTMKAC 1 cut(s) 1096
XspI CTAG 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.