Prupe.8G212300_v2.0.a1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0015082
Physical Location & Seq
Forward (+)
19593029 .. 19595785
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G212300.1

Sequence Viewer

Length: 1632 bp
ATGGGGATATTTGATTATAAAAGGCTAGAAAGTTCATCAAATCCTCGAAGGATTCTTCGCCAAACTCCATTGCCAATGGAGGGATCGCCTAGGCTCGGCCCAAAAGGTCTAATTAAGAAGCACGAGTTTGTAAGAATACTAATTCAATGCCTATATTCAATTGGATATGAGAAGTCTGCCTCATGCTTGGAGTTGGAGTCTGGCATTTCCTGCAAATCCGATGATTTTCAATTGCTCGAATCACAGATATCGAGCGGGAATTGGGATGGTTGTGTTGATACCCTTAATGCCATTAAGGATTTGACCGATGACACAAGAGCTGCTGCTTTGTTTCTTGTGTTCAAACAGTGCCTTTTGGAGTATTTGAGTTGTGGGGATGACAAATTGGCTGTGGCCATTTTGCAGAAAGAGGTGTCAGCTTTACAGGTGGACAGAGATAAGGTTCACAACCTTGCTCGGAGTATCCTTTCCTGGAAGGAAGTGGAGCAGAGCAAGATAGACGATAATGCTGTTCGAGAATTGCGAAAAAAACTGTTGCAAGAATTGGAAAAATGGCTTCCTTCACCAATTACGCTGCCCGAGCACAGATTGGAACATCTGGTTGAAACTGCTGTTTTGTCCCAGATTGAGTCATGTATGTATCATAATTTGTCGGATGAAGTTTCACTTTATGCAGACCATTCCTGTAGTAGGGATCAGATCCCTACAGAGACTGTTCAGATTTTGACTGAGCATAAGAATGAAGTTTGGTTTGTGCAATTTTCTAATGATGGGGAGTACTTAGCCTCTTCGTCAAGCGATTGCACAGCCATCATATGGAAGGTGCCGGAGGATGGTAGGGTGACATTAAAACATACCCTACGGAGTCACCACAATCCAGTATCCTTTGTAGCGTGGAGCCCTGATGACTCAAAGTTGCTTACATGTGGAAATGTTGAAGTCCTCAAGCTATGGGATGTTGAAACAGGTACATGCAAGCATACATTTGGGGATCCTGGCTTCATTGTCAGCTCATGTGCTTGGTTTCCCGACTCAAAGCGACTTGTGTGTGGCAGTTCCGACCCTGAAAAGGGCATCTACATGTGGGATTGTGATGGCAATGAGTTAAAAGCATGGAGAGGGATGAGGATGCCCAAGATTTTAGATCTTGCAGTGACACCAGATGGGCAAAATCTTATCAGCATCTTCTCTGATAAAGAAATTCGGATTTTAAACGTGGGAACAAATGCCGAACGAGTCATCTCTGAAGACCATTCTATCACATCCCTTTCAATTTCTGGAGATAGTAAGTTCTTTATTGTCAACCTGAATAGCCAGGAGATTCATATGTGGGATGTTGCTGGTGAATGGGTGAGGCCACAGAAGTATATGGGCCACAAGCAGAGCAAGTATGTGATACGCTCCTGCTTTGGTGGATTGAATAGCACATTCATTGCCAGTGGTAGTGAGAACGCACAGGTATACATCTGGAACCGGCGAAATCATAGGCCAATTGAGGTTTTGCGTGGGCATTCAATGACTGTGAACTGTGTGAGTTGGAATCATAGGAGACCTCAATTGCTGGCATCGGCGAGTGATGATCATTCTATCCGTATATGGGGATCTAGCCTTTCTAATAAGATTCAACCTTAG

Protein Analysis

544

Amino Acids

61.46

Weight (kDa)

6.0

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43920
fragaria_vesca FvH4_2g20200
malus_domestica MD10G1173700.v1.1
prunus_persica Prupe.8G212300_v2.0.a1
pyrus_communis pycom05g17030 pycom10g14980
rosa_chinensis RchiOBHm_Chr6g0285971
rosa_laevigata RLG00000012593
rosa_multiflora Rmu_sc0005513.1_g000002
rosa_roxburghii Rroxscaffold_7G00182560
rosa_rugosa Rorug06G0177800
rosa_samantha Rh6AG289900 Rh6BG291900 Rh6CG292900 Rh6DG285000
rosa_wichuraiana Rw6G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
AccB1I GGYRCC 1 cut(s) 823
AccB7I CCANNNNNTGG 1 cut(s) 816
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 1461
AciI CCGC 1 cut(s) 255
AclWI GGATC 6 cut(s) 91, 694, 702, 986, 999, 1609
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 1200
AcuI CTGAAG 1 cut(s) 1266
AfaI GTAC 2 cut(s) 779, 970
AfiI CCNNNNNNNGG 8 cut(s) 80, 95, 690, 816, 833, 1069, 1070, 1597
AflIII ACRYGT 2 cut(s) 923, 1080
AjnI CCWGG 3 cut(s) 470, 994, 1314
AluBI AGCT 4 cut(s) 320, 419, 949, 1011
AluI AGCT 4 cut(s) 320, 419, 949, 1011
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 704, 1543
AlwI GGATC 6 cut(s) 91, 694, 702, 986, 999, 1609
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 5 cut(s) 97, 393, 1355, 1372, 1487
ApeKI GCWGC 3 cut(s) 320, 323, 574
ApoI RAATTY 1 cut(s) 1200
AspA2I CCTAGG 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 98, 1372
AsuHPI GGTGA 5 cut(s) 555, 853, 860, 1355, 1363
AvaI CYCGRG 1 cut(s) 578
AvrII CCTAGG 1 cut(s) 89
BalI TGGCCA 1 cut(s) 395
BamHI GGATCC 1 cut(s) 991
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 902
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 1254
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 3 cut(s) 307, 310, 561
BccI CCATC 6 cut(s) 260, 764, 818, 827, 1088, 1157
BciT130I CCWGG 3 cut(s) 472, 996, 1316
BciVI GTATCC 2 cut(s) 473, 892
BclI TGATCA 1 cut(s) 1579
BcoDI GTCTC 2 cut(s) 704, 1543
BfaI CTAG 3 cut(s) 26, 90, 1605
BfmI CTRYAG 2 cut(s) 685, 705
BfuI GTATCC 2 cut(s) 473, 892
BglII AGATCT 1 cut(s) 1144
BisI GCNGC 3 cut(s) 321, 324, 575
BlnI CCTAGG 1 cut(s) 89
BlsI GCNGC 3 cut(s) 322, 325, 576
BmcAI AGTACT 1 cut(s) 779
Bme1390I CCNGG 3 cut(s) 472, 996, 1316
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 98, 1372
BmiI GGNNCC 4 cut(s) 825, 899, 993, 1472
BmrFI CCNGG 3 cut(s) 472, 996, 1316
BmsI GCATC 4 cut(s) 1083, 1119, 1191, 1574
BpiI GAAGAC 1 cut(s) 1254
BpmI CTGGAG 1 cut(s) 1299
BpuEI CTTGAG 1 cut(s) 929
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 8 cut(s) 80, 95, 690, 816, 833, 1069, 1070, 1597
Bse118I RCCGGY 1 cut(s) 1473
Bse1I ACTGG 2 cut(s) 878, 1437
Bse3DI GCAATG 3 cut(s) 68, 1105, 1431
BseBI CCWGG 3 cut(s) 472, 996, 1316
BseDI CCNNGG 1 cut(s) 89
BseGI GGATG 9 cut(s) 271, 382, 661, 838, 961, 1128, 1134, 1262, 1339
BseLI CCNNNNNNNGG 8 cut(s) 80, 95, 690, 816, 833, 1069, 1070, 1597
BseMI GCAATG 3 cut(s) 68, 1105, 1431
BseMII CTCAG 1 cut(s) 720
BseNI ACTGG 2 cut(s) 878, 1437
BseXI GCAGC 3 cut(s) 307, 310, 561
BshFI GGCC 5 cut(s) 99, 395, 1357, 1374, 1489
BshNI GGYRCC 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 2 cut(s) 827, 1474
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 8 cut(s) 80, 95, 690, 816, 833, 1069, 1070, 1597
BsmAI GTCTC 2 cut(s) 704, 1543
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 1510
BsnI GGCC 5 cut(s) 99, 395, 1357, 1374, 1489
Bso31I GGTCTC 1 cut(s) 1543
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 2 cut(s) 585, 902
Bsp143I GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
BspACI CCGC 1 cut(s) 255
BspANI GGCC 5 cut(s) 99, 395, 1357, 1374, 1489
BspCNI CTCAG 1 cut(s) 721
BspLI GGNNCC 4 cut(s) 825, 899, 993, 1472
BspPI GGATC 6 cut(s) 91, 694, 702, 986, 999, 1609
BspT107I GGYRCC 1 cut(s) 823
BspTNI GGTCTC 1 cut(s) 1543
BsrBI CCGCTC 1 cut(s) 255
BsrDI GCAATG 3 cut(s) 68, 1105, 1431
BsrFI RCCGGY 1 cut(s) 1473
BsrI ACTGG 2 cut(s) 878, 1437
BssAI RCCGGY 1 cut(s) 1473
BssECI CCNNGG 1 cut(s) 89
BssMI GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
BssNAI GTATAC 1 cut(s) 1462
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 89
Bst1107I GTATAC 1 cut(s) 1462
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 472, 996, 1316
Bst4CI ACNGT 5 cut(s) 348, 534, 715, 1522, 1529
Bst6I CTCTTC 1 cut(s) 793
BstAPI GCANNNNNTGC 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 977, 1563
BstDEI CTNAG 3 cut(s) 729, 781, 1629
BstENI CCTNNNNNAGG 1 cut(s) 688
BstF5I GGATG 9 cut(s) 271, 382, 661, 838, 961, 1128, 1134, 1262, 1339
BstKTI GATC 7 cut(s) 86, 697, 702, 994, 1147, 1582, 1604
BstMAI GTCTC 2 cut(s) 704, 1543
BstMBI GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
BstMWI GCNNNNNNNGC 2 cut(s) 210, 580
BstNI CCWGG 3 cut(s) 472, 996, 1316
BstNSI RCATGY 3 cut(s) 927, 975, 1084
BstSCI CCNGG 3 cut(s) 470, 994, 1314
BstSFI CTRYAG 2 cut(s) 685, 705
BstV1I GCAGC 3 cut(s) 307, 310, 561
BstV2I GAAGAC 1 cut(s) 1254
BstX2I RGATCY 4 cut(s) 699, 991, 1144, 1601
BstYI RGATCY 4 cut(s) 699, 991, 1144, 1601
BstZ17I GTATAC 1 cut(s) 1462
BsuI GTATCC 2 cut(s) 473, 892
BsuRI GGCC 5 cut(s) 99, 395, 1357, 1374, 1489
BtsCI GGATG 9 cut(s) 271, 382, 661, 838, 961, 1128, 1134, 1262, 1339
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 3 cut(s) 353, 1158, 1444
Cac8I GCNNGC 2 cut(s) 977, 1563
CaiI CAGNNNCTG 1 cut(s) 713
Cfr10I RCCGGY 1 cut(s) 1473
Cfr13I GGNCC 2 cut(s) 98, 1372
Csp6I GTAC 2 cut(s) 778, 969
CviAII CATG 7 cut(s) 183, 633, 924, 972, 1014, 1081, 1113
CviQI GTAC 2 cut(s) 778, 969
DdeI CTNAG 3 cut(s) 729, 781, 1629
DpnI GATC 7 cut(s) 85, 696, 701, 993, 1146, 1581, 1603
DpnII GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
DraI TTTAAA 1 cut(s) 1212
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 793
EarI CTCTTC 1 cut(s) 793
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 902
Eco31I GGTCTC 1 cut(s) 1543
Eco32I GATATC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 1266
Eco88I CYCGRG 1 cut(s) 578
EcoNI CCTNNNNNAGG 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 470, 994, 1314
EcoRV GATATC 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 902
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 7 cut(s) 186, 636, 927, 975, 1017, 1084, 1116
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 1 cut(s) 604
FatI CATG 7 cut(s) 182, 632, 923, 971, 1013, 1080, 1112
FauI CCCGC 1 cut(s) 248
FauNDI CATATG 2 cut(s) 815, 1326
FbaI TGATCA 1 cut(s) 1579
FblI GTMKAC 1 cut(s) 1461
Fnu4HI GCNGC 3 cut(s) 321, 324, 575
FokI GGATG 9 cut(s) 278, 389, 668, 845, 968, 1135, 1141, 1249, 1346
FriOI GRGCYC 1 cut(s) 902
Fsp4HI GCNGC 3 cut(s) 321, 324, 575
FspBI CTAG 3 cut(s) 26, 90, 1605
GluI GCNGC 3 cut(s) 321, 324, 575
GsuI CTGGAG 1 cut(s) 1299
HaeIII GGCC 5 cut(s) 99, 395, 1357, 1374, 1489
HapII CCGG 2 cut(s) 827, 1474
Hin1II CATG 7 cut(s) 186, 636, 927, 975, 1017, 1084, 1116
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HpaII CCGG 2 cut(s) 827, 1474
HphI GGTGA 5 cut(s) 555, 853, 860, 1355, 1363
Hpy166II GTNNAC 5 cut(s) 430, 445, 1303, 1462, 1525
Hpy188I TCNGA 9 cut(s) 220, 459, 655, 699, 720, 1060, 1192, 1206, 1246
Hpy188III TCNNGA 4 cut(s) 515, 1028, 1278, 1468
Hpy8I GTNNAC 5 cut(s) 430, 445, 1303, 1462, 1525
HpyAV CCTTC 4 cut(s) 42, 469, 570, 814
HpyCH4III ACNGT 5 cut(s) 348, 534, 715, 1522, 1529
HpyCH4IV ACGT 1 cut(s) 1215
HpyCH4V TGCA 8 cut(s) 213, 403, 538, 674, 757, 804, 975, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 580
HpyF3I CTNAG 3 cut(s) 729, 781, 1629
HpySE526I ACGT 1 cut(s) 1215
Hsp92II CATG 7 cut(s) 186, 636, 927, 975, 1017, 1084, 1116
Ksp22I TGATCA 1 cut(s) 1579
Kzo9I GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
LmnI GCTCC 3 cut(s) 484, 897, 1406
Lsp1109I GCAGC 3 cut(s) 307, 310, 561
LweI GCATC 4 cut(s) 1083, 1119, 1191, 1574
MaeI CTAG 3 cut(s) 26, 90, 1605
MaeII ACGT 1 cut(s) 1215
MaeIII GTNAC 3 cut(s) 841, 866, 1153
MalI GATC 7 cut(s) 85, 696, 701, 993, 1146, 1581, 1603
MbiI CCGCTC 1 cut(s) 255
MboI GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
MboII GAAGA 4 cut(s) 47, 780, 1177, 1259
MfeI CAATTG 4 cut(s) 159, 230, 1491, 1556
MflI RGATCY 4 cut(s) 699, 991, 1144, 1601
MhlI GDGCHC 2 cut(s) 585, 902
MlsI TGGCCA 1 cut(s) 395
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 6 cut(s) 206, 638, 874, 902, 1025, 1245
MmeI TCCRAC 4 cut(s) 174, 633, 1083, 1517
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
MseI TTAA 6 cut(s) 114, 285, 294, 848, 1106, 1211
MslI CAYNNNNRTG 2 cut(s) 738, 1079
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 2 cut(s) 827, 1474
MspR9I CCNGG 3 cut(s) 472, 996, 1316
MunI CAATTG 4 cut(s) 159, 230, 1491, 1556
Mva1269I GAATGC 1 cut(s) 1510
MvaI CCWGG 3 cut(s) 472, 996, 1316
MwoI GCNNNNNNNGC 2 cut(s) 210, 580
NdeI CATATG 2 cut(s) 815, 1326
NdeII GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
NlaIII CATG 7 cut(s) 186, 636, 927, 975, 1017, 1084, 1116
NlaIV GGNNCC 4 cut(s) 825, 899, 993, 1472
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 3 cut(s) 841, 866, 1153
NspI RCATGY 3 cut(s) 927, 975, 1084
PciI ACATGT 2 cut(s) 923, 1080
PcsI WCGNNNNNNNCGW 1 cut(s) 520
PctI GAATGC 1 cut(s) 1510
PfeI GAWTC 5 cut(s) 52, 239, 1321, 1540, 1621
PflMI CCANNNNNTGG 1 cut(s) 816
PfoI TCCNGGA 1 cut(s) 470
PkrI GCNGC 3 cut(s) 322, 325, 576
PleI GAGTC 6 cut(s) 205, 637, 873, 902, 1025, 1244
PpsI GAGTC 6 cut(s) 205, 637, 873, 902, 1025, 1244
PscI ACATGT 2 cut(s) 923, 1080
PsiI TTATAA 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 470, 994, 1314
PspGI CCWGG 3 cut(s) 470, 994, 1314
PspN4I GGNNCC 4 cut(s) 825, 899, 993, 1472
PspPI GGNCC 2 cut(s) 98, 1372
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 4 cut(s) 699, 991, 1144, 1601
RsaI GTAC 2 cut(s) 779, 970
RsaNI GTAC 2 cut(s) 778, 969
RseI CAYNNNNRTG 2 cut(s) 738, 1079
SaqAI TTAA 6 cut(s) 114, 285, 294, 848, 1106, 1211
SatI GCNGC 3 cut(s) 321, 324, 575
Sau3AI GATC 7 cut(s) 83, 694, 699, 991, 1144, 1579, 1601
Sau96I GGNCC 2 cut(s) 98, 1372
ScaI AGTACT 1 cut(s) 779
SchI GAGTC 6 cut(s) 206, 638, 874, 902, 1025, 1245
ScrFI CCNGG 3 cut(s) 472, 996, 1316
SduI GDGCHC 2 cut(s) 585, 902
SfaNI GCATC 4 cut(s) 1083, 1119, 1191, 1574
SfcI CTRYAG 2 cut(s) 685, 705
SmiMI CAYNNNNRTG 2 cut(s) 738, 1079
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SsiI CCGC 1 cut(s) 255
SspMI CTAG 3 cut(s) 26, 90, 1605
StyD4I CCNGG 3 cut(s) 470, 994, 1314
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 5 cut(s) 348, 534, 715, 1522, 1529
TaiI ACGT 1 cut(s) 1218
TaqI TCGA 4 cut(s) 46, 237, 251, 514
TaqII GACCGA 1 cut(s) 320
TatI WGTACW 1 cut(s) 777
TfiI GAWTC 5 cut(s) 52, 239, 1321, 1540, 1621
Tru1I TTAA 6 cut(s) 114, 285, 294, 848, 1106, 1211
Tru9I TTAA 6 cut(s) 114, 285, 294, 848, 1106, 1211
TscAI CASTG 3 cut(s) 353, 1158, 1444
TseFI GTSAC 3 cut(s) 841, 866, 1153
TseI GCWGC 3 cut(s) 320, 323, 574
Tsp45I GTSAC 3 cut(s) 841, 866, 1153
TspDTI ATGAA 6 cut(s) 24, 672, 756, 991, 1313, 1420
TspGWI ACGGA 2 cut(s) 877, 1580
TspRI CASTG 3 cut(s) 353, 1158, 1444
Van91I CCANNNNNTGG 1 cut(s) 816
XagI CCTNNNNNAGG 1 cut(s) 688
XapI RAATTY 1 cut(s) 1200
XceI RCATGY 3 cut(s) 927, 975, 1084
XmaJI CCTAGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 1461
XspI CTAG 3 cut(s) 26, 90, 1605
ZrmI AGTACT 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.