Rroxscaffold_7G00182560

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015082
Physical Location & Seq
Reverse (-)
21633525 .. 21637047
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182560.1

Sequence Viewer

Length: 1575 bp
ATGTCGATGGAGGGTTCGACTAGGCTCGGGCCAAGAGGCCTAATAAAGAAGCATGAGTTTGTAAGAATAATAATTCAGTGCCTATATTCGATGGGCTATGGGAAGGCCGCTTCATGCTTAGAGTTGGAGTCTGGCATTGCTTGCCAATCTGATTATTTTCAGATGCTGGAATCGAAGATATTGAGCGAGAATTGGAATGATTGTATTGATATCCTTAAAGCCATTAAGGATTTGACAGAAGAAACAAGAGCTACTGCTTTGTTTCTAATATTGAAACAGTCACTTTTGGAGTGTTCAAAGTGCGGGGATGACAAATTGGCTTTAGACATTTTGCAGAAAGAGGTTTCAGCATTACAGATGGGCAGAGATAAGGTTCACAACCTTGCTCACATTATCCTTTTCTCGAAGGATATGGAGCAGGGTAAGATGGACAACAATGAGGTTTGCGAATTGCGAAAAAAGCTGTTGCAAGAATTGGAAAATTTGCTTCCTCCACCAATTACACTACCTCGGTGCAGGTTGGAACATCTGGTTGAAACTGCTGTTTTGGCCCAGATTGATTCATGTATGTATCATAATTTTTCGGATGCTGTTTCACTTTATGCAGACCATTTGTGCGGTAGGGATCAGATCCCTACTGAGACTGTACAGGTTTTGACTGAACATAGGGACGAAGCTTGGTTTGTACAATTTTCTCATAATGGGGAGTATTTAGCTTCTTCATCAAGTGATTGCTCGGCCATCATATGGAAGGTGCTGGAAGATGACAGCGTGATATTAAAACATACCCTATGGAGCCATGGTAAACCAGTATCCTTTGTGGCATGGAGCCCCGATGACTCAAAGTTGCTTACGTGTGGAAATGGTGAAGTCCTCAAGCTATGGGATGTGGAAACAGGTACATGCAAGCATACATTTCAAGAGCATGGCTTCATTGTCAGCTCATGTGCTTGGTTTCCCGACTCAAAGCGACTTGTGTGTGGCAGTTCTGACCCTGAAAAAGGTATCTACATGTGGGATTGTCATGGTAACGAGTTAAAAGCATGGAAAGGTATGAGGATGCCCAAGATTTTAGATCTTGCTGTAACACCAGATGGGGAAAATCTTATCAGCATTTTCTCTGATAAAGAAATTCGGATTTTGAATGTGGGAACAAATGCTGAGCGAGTTATCTCTGAAGACCATTCTATCACATCCCTCTCGATTTCTAGAGATAGTGAGTACTTTATCGTCAACCTAAACAGCCAGGAGATCCATATGTGGGATGTTGCTGGGAAACTGGAGAAGCCACTGAAGTATGTGGGTCACAGGCAGAGCAAATACGTAATACGCTCCTGCTTCGGTGGAGTGAATAGTAAATTTATTGCCAGCGGTAGTGAGAACTCACAGGTCTACATTTGGAATCGGCGAAACTCTATGCCAATTGAGGTTTTGTGTGGGCATTCAATGACTGTGAACTGTGTGAGTTGGAATCTTAGGAGACCTCAAATGCTGGCATCAGCTAGTGATGATCGTACCATCCGTATATGGGGATCTAGTCTATCTAAAAAGACTCAACCTGAGAAACTCAGTTGA

Protein Analysis

524

Amino Acids

59.24

Weight (kDa)

5.89

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 7 - 50 4.4e-22 WDR26 Lis-homology motif
Beta-prop_CAF1B_HIR1 PF24105 208 - 339 1.9e-11 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 214 - 331 8.7e-09 WDR55
Beta-prop_EML PF23409 214 - 385 2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 214 - 336 3.8e-21 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 214 - 380 8.1e-20 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 214 - 349 1.1e-10 TEP-1 second beta-propeller
Beta-prop_IFT122_1st PF23381 215 - 328 7.1e-06 IFT122 first beta-propeller
Beta-prop_Aladin PF25460 216 - 337 2.4e-14 Aladin seven-bladed propeller
Beta-prop_WDR5 PF25175 219 - 510 5.1e-48 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 222 - 468 3.2e-31 THOC3 beta-propeller domain
WD40_Gbeta PF25391 238 - 332 1.5e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 255 - 381 5.1e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 262 - 423 2.5e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 267 - 327 3.3e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 274 - 425 1.7e-11 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 315 - 424 1.8e-06 WDR55
Beta-prop_WDR3_2nd PF25172 351 - 471 8.1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 365 - 510 3.3e-07 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 390 - 511 5e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 450 - 514 4.1e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 451 - 515 4.7e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 451 - 519 4.1e-12 THOC3 beta-propeller domain
WD40 PF00400 474 - 510 2.4e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 475 - 514 3.8e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43920
fragaria_vesca FvH4_2g20200
malus_domestica MD10G1173700.v1.1
prunus_persica Prupe.8G212300_v2.0.a1
pyrus_communis pycom05g17030 pycom10g14980
rosa_chinensis RchiOBHm_Chr6g0285971
rosa_laevigata RLG00000012593
rosa_multiflora Rmu_sc0005513.1_g000002
rosa_roxburghii Rroxscaffold_7G00182560
rosa_rugosa Rorug06G0177800
rosa_samantha Rh6AG289900 Rh6BG291900 Rh6CG292900 Rh6DG285000
rosa_wichuraiana Rw6G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 507
AccB7I CCANNNNNTGG 1 cut(s) 747
AccI GTMKAC 1 cut(s) 1392
AciI CCGC 4 cut(s) 108, 303, 618, 1371
AclWI GGATC 4 cut(s) 625, 633, 1246, 1540
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 3 cut(s) 481, 1131, 1358
AcuI CTGAAG 2 cut(s) 1197, 1313
AfaI GTAC 5 cut(s) 648, 687, 901, 1223, 1516
AfiI CCNNNNNNNGG 4 cut(s) 747, 1001, 1261, 1528
AflIII ACRYGT 2 cut(s) 854, 1011
AgsI TTSAA 6 cut(s) 274, 297, 536, 920, 1144, 1446
AjnI CCWGG 1 cut(s) 1245
AluBI AGCT 7 cut(s) 251, 463, 677, 716, 880, 942, 1502
AluI AGCT 7 cut(s) 251, 463, 677, 716, 880, 942, 1502
Alw26I GTCTC 2 cut(s) 635, 1474
AlwI GGATC 4 cut(s) 625, 633, 1246, 1540
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 5 cut(s) 29, 37, 105, 549, 738
ApoI RAATTY 3 cut(s) 481, 1131, 1358
AspS9I GGNCC 2 cut(s) 29, 550
AsuHPI GGTGA 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 26
BanII GRGCYC 1 cut(s) 833
BbsI GAAGAC 1 cut(s) 1185
BccI CCATC 6 cut(s) 85, 352, 421, 749, 1088, 1526
BciT130I CCWGG 1 cut(s) 1247
BciVI GTATCC 1 cut(s) 823
BcoDI GTCTC 2 cut(s) 635, 1474
BfaI CTAG 4 cut(s) 21, 1209, 1503, 1536
BfuAI ACCTGC 1 cut(s) 507
BfuI GTATCC 1 cut(s) 823
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 1 cut(s) 108
BlpI GCTNAGC 1 cut(s) 1161
BlsI GCNGC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 1223
Bme1390I CCNGG 1 cut(s) 1247
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 29, 550
BmiI GGNNCC 2 cut(s) 797, 830
BmrFI CCNGG 1 cut(s) 1247
BmsI GCATC 4 cut(s) 153, 577, 1050, 1505
BpiI GAAGAC 1 cut(s) 1185
BpmI CTGGAG 1 cut(s) 1301
Bpu1102I GCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 1 cut(s) 860
BsaAI YACGTR 2 cut(s) 855, 1324
BsaI GGTCTC 1 cut(s) 1474
BsaJI CCNNGG 2 cut(s) 509, 799
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 4 cut(s) 747, 1001, 1261, 1528
Bse1I ACTGG 2 cut(s) 809, 1284
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 2 cut(s) 509, 799
BseGI GGATG 7 cut(s) 313, 592, 892, 1065, 1193, 1270, 1518
BseLI CCNNNNNNNGG 4 cut(s) 747, 1001, 1261, 1528
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 3 cut(s) 630, 1152, 1551
BseNI ACTGG 2 cut(s) 809, 1284
BseYI CCCAGC 1 cut(s) 1271
BsgI GTGCAG 1 cut(s) 535
BshFI GGCC 5 cut(s) 31, 39, 107, 551, 740
BsiHKCI CYCGRG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 4 cut(s) 747, 1001, 1261, 1528
BsmAI GTCTC 2 cut(s) 635, 1474
BsmFI GGGAC 1 cut(s) 683
BsmI GAATGC 1 cut(s) 1441
BsnI GGCC 5 cut(s) 31, 39, 107, 551, 740
Bso31I GGTCTC 1 cut(s) 1474
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 833
Bsp1407I TGTACA 2 cut(s) 646, 685
Bsp143I GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
Bsp1720I GCTNAGC 1 cut(s) 1161
Bsp19I CCATGG 1 cut(s) 799
BspACI CCGC 4 cut(s) 108, 303, 618, 1371
BspANI GGCC 5 cut(s) 31, 39, 107, 551, 740
BspCNI CTCAG 3 cut(s) 631, 1153, 1552
BspLI GGNNCC 2 cut(s) 797, 830
BspMI ACCTGC 1 cut(s) 507
BspPI GGATC 4 cut(s) 625, 633, 1246, 1540
BspTNI GGTCTC 1 cut(s) 1474
BsrDI GCAATG 1 cut(s) 135
BsrGI TGTACA 2 cut(s) 646, 685
BsrI ACTGG 2 cut(s) 809, 1284
BssECI CCNNGG 2 cut(s) 509, 799
BssMI GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 4 cut(s) 279, 646, 1453, 1460
BstAPI GCANNNNNTGC 1 cut(s) 141
BstAUI TGTACA 2 cut(s) 646, 685
BstBAI YACGTR 2 cut(s) 855, 1324
BstC8I GCNNGC 4 cut(s) 142, 908, 1369, 1494
BstDEI CTNAG 6 cut(s) 118, 639, 1161, 1475, 1560, 1568
BstDSI CCRYGG 1 cut(s) 799
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 7 cut(s) 313, 592, 892, 1065, 1193, 1270, 1518
BstKTI GATC 6 cut(s) 628, 633, 1078, 1254, 1513, 1535
BstMAI GTCTC 2 cut(s) 635, 1474
BstMBI GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
BstMWI GCNNNNNNNGC 3 cut(s) 141, 460, 548
BstNI CCWGG 1 cut(s) 1247
BstNSI RCATGY 2 cut(s) 906, 1015
BstSCI CCNGG 1 cut(s) 1245
BstSNI TACGTA 1 cut(s) 1324
BstV2I GAAGAC 1 cut(s) 1185
BstX2I RGATCY 4 cut(s) 630, 1075, 1251, 1532
BstYI RGATCY 4 cut(s) 630, 1075, 1251, 1532
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 5 cut(s) 31, 39, 107, 551, 740
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 7 cut(s) 313, 592, 892, 1065, 1193, 1270, 1518
BtsIMutI CAGTG 2 cut(s) 83, 1289
BveI ACCTGC 1 cut(s) 507
Cac8I GCNNGC 4 cut(s) 142, 908, 1369, 1494
CaiI CAGNNNCTG 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 29, 550
Csp6I GTAC 5 cut(s) 647, 686, 900, 1222, 1515
CviQI GTAC 5 cut(s) 647, 686, 900, 1222, 1515
DdeI CTNAG 6 cut(s) 118, 639, 1161, 1475, 1560, 1568
DpnI GATC 6 cut(s) 627, 632, 1077, 1253, 1512, 1534
DpnII GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
EaeI YGGCCR 1 cut(s) 738
Eco105I TACGTA 1 cut(s) 1324
Eco130I CCWWGG 1 cut(s) 799
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 1474
Eco32I GATATC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 1197, 1313
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 1245
EcoRV GATATC 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 1 cut(s) 799
FaqI GGGAC 1 cut(s) 683
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 2 cut(s) 746, 1257
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 7 cut(s) 320, 599, 899, 1072, 1180, 1277, 1505
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 4 cut(s) 21, 1209, 1503, 1536
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 1275
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 5 cut(s) 31, 39, 107, 551, 740
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 8 cut(s) 128, 170, 560, 839, 962, 1402, 1471, 1552
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 5 cut(s) 376, 806, 1234, 1393, 1456
Hpy188I TCNGA 8 cut(s) 151, 162, 586, 630, 991, 1123, 1137, 1177
Hpy188III TCNNGA 5 cut(s) 403, 920, 959, 1201, 1209
Hpy8I GTNNAC 5 cut(s) 376, 806, 1234, 1393, 1456
HpyAV CCTTC 3 cut(s) 97, 400, 745
HpyCH4III ACNGT 4 cut(s) 279, 646, 1453, 1460
HpyCH4IV ACGT 2 cut(s) 854, 1323
HpyCH4V TGCA 5 cut(s) 334, 469, 516, 605, 906
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 460, 548
HpyF3I CTNAG 6 cut(s) 118, 639, 1161, 1475, 1560, 1568
HpySE526I ACGT 2 cut(s) 854, 1323
Kzo9I GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
LmnI GCTCC 4 cut(s) 415, 795, 828, 1337
LweI GCATC 4 cut(s) 153, 577, 1050, 1505
MaeI CTAG 4 cut(s) 21, 1209, 1503, 1536
MaeII ACGT 2 cut(s) 854, 1323
MaeIII GTNAC 4 cut(s) 279, 1028, 1084, 1304
MalI GATC 6 cut(s) 627, 632, 1077, 1253, 1512, 1534
MboI GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
MboII GAAGA 5 cut(s) 187, 251, 711, 773, 1190
MfeI CAATTG 1 cut(s) 1422
MflI RGATCY 4 cut(s) 630, 1075, 1251, 1532
MhlI GDGCHC 1 cut(s) 833
MlyI GAGTC 4 cut(s) 137, 833, 956, 1546
MmeI TCCRAC 3 cut(s) 105, 501, 1448
MseI TTAA 4 cut(s) 216, 225, 779, 1037
MspA1I CMGCKG 1 cut(s) 1371
MspR9I CCNGG 1 cut(s) 1247
MunI CAATTG 1 cut(s) 1422
Mva1269I GAATGC 1 cut(s) 1441
MvaI CCWGG 1 cut(s) 1247
MwoI GCNNNNNNNGC 3 cut(s) 141, 460, 548
NcoI CCATGG 1 cut(s) 799
NdeI CATATG 2 cut(s) 746, 1257
NdeII GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
NlaIV GGNNCC 2 cut(s) 797, 830
NmeAIII GCCGAG 1 cut(s) 716
NmuCI GTSAC 2 cut(s) 279, 1304
NspI RCATGY 2 cut(s) 906, 1015
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 1519
PctI GAATGC 1 cut(s) 1441
PfeI GAWTC 4 cut(s) 170, 560, 1402, 1471
PflMI CCANNNNNTGG 1 cut(s) 747
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 4 cut(s) 136, 833, 956, 1546
PpsI GAGTC 4 cut(s) 136, 833, 956, 1546
Ppu21I YACGTR 2 cut(s) 855, 1324
PscI ACATGT 1 cut(s) 1011
Psp6I CCWGG 1 cut(s) 1245
PspFI CCCAGC 1 cut(s) 1271
PspGI CCWGG 1 cut(s) 1245
PspN4I GGNNCC 2 cut(s) 797, 830
PspPI GGNCC 2 cut(s) 29, 550
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 4 cut(s) 630, 1075, 1251, 1532
RsaI GTAC 5 cut(s) 648, 687, 901, 1223, 1516
RsaNI GTAC 5 cut(s) 647, 686, 900, 1222, 1515
SaqAI TTAA 4 cut(s) 216, 225, 779, 1037
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 6 cut(s) 625, 630, 1075, 1251, 1510, 1532
Sau96I GGNCC 2 cut(s) 29, 550
ScaI AGTACT 1 cut(s) 1223
SchI GAGTC 4 cut(s) 137, 833, 956, 1546
ScrFI CCNGG 1 cut(s) 1247
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 4 cut(s) 153, 577, 1050, 1505
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
SnaBI TACGTA 1 cut(s) 1324
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 4 cut(s) 108, 303, 618, 1371
SspI AATATT 1 cut(s) 270
SspMI CTAG 4 cut(s) 21, 1209, 1503, 1536
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 1245
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 4 cut(s) 279, 646, 1453, 1460
TaiI ACGT 2 cut(s) 857, 1326
TaqI TCGA 6 cut(s) 5, 17, 89, 173, 404, 1202
TatI WGTACW 3 cut(s) 646, 685, 1221
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 4 cut(s) 170, 560, 1402, 1471
Tru1I TTAA 4 cut(s) 216, 225, 779, 1037
Tru9I TTAA 4 cut(s) 216, 225, 779, 1037
TscAI CASTG 2 cut(s) 83, 1296
TseFI GTSAC 2 cut(s) 279, 1304
Tsp45I GTSAC 2 cut(s) 279, 1304
TspDTI ATGAA 4 cut(s) 102, 552, 711, 922
TspGWI ACGGA 1 cut(s) 1511
TspRI CASTG 2 cut(s) 83, 1296
Van91I CCANNNNNTGG 1 cut(s) 747
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 3 cut(s) 481, 1131, 1358
XbaI TCTAGA 1 cut(s) 1208
XceI RCATGY 2 cut(s) 906, 1015
XmiI GTMKAC 1 cut(s) 1392
XspI CTAG 4 cut(s) 21, 1209, 1503, 1536
ZrmI AGTACT 1 cut(s) 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.