RLG00000012593

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015082
Physical Location & Seq
Reverse (-)
20306183 .. 20310234
4052 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012593

Sequence Viewer

Length: 1494 bp
ATGTCGATGGAGGGTTTGACTAGGCTCGGGCCAAGAGGCCTAATAAAGAAGCATGAGTTTGTAAGAATAATAATTCAGTGCCTATATTCGATGGGCTATGGGAAGGCCGCTTCATGCTTAGAGTTGGAGTCTGGCATTGCTTGCCAATCTGATTATTTTCAGATGCTGGAATCGAAGATATTGAGCGGGAATTGGAATGATTGTATTGGTATCCTTAATGCCATTAAGGATTTGACAGAAGAAACAAGAGCTACTGCTTTGTTTCTAGTATTCAAACAGTCACTTTTGGAGTGTTCAAAGTGCGGGGATGACAAATTGGCTTTAGACATTTTGCAGAAAGAGGTTTCAGCATTACAGATGGGCAGAGATAAGGTTCACAACCTTGCTCACAGTATCCTTTTCTCGAAGGATATGGAGCAGGGTAAGATGGACAACAATGGGGTTTGCGAATTGCGCAAAAAGCTGTTGCAAGAATTGGAAAATTTGCTTCCTCCACCAATTACACTACCTTGGCGTAGGTTGGAACATCTGGTTGAAACTGCTGTTTTGGCCCAGATTGATTCATGTATGTATCATAATTTTTCGGGTGCTGTTTCACTTTATGCAGACCATTTGTGCGGTAGGGATCAGATCCCTACTGAGACTGTACAGATTTTGACTGAACATAGGGACGAAGCTTGGTTTGTACAATTTTCTCATAATGGGGAGTATTTAGCTTCTTCATCAAGTGATTGCACGGCCATCATATGGAAGGTGCTGGAAGATGACAGCGTGATATTAAAACATACCCTATGGAGCCACGATAAACCAGTATCCTTTGTGGCATGGAGCCCCGATGACTCAAAGTTGCTTACGTGTGGAAATGGTGAAGTCCTCAAGCTATGGGATGTGGAAACAGGTACATGCAAGCATACATTTGGAGAGCATGGCTTCATTGTCAGCTCATGTGCTTGGTTTCCCGACTCAAAGCGACTTGTGTGTGGCAGTTCTGACCCTGAAAAAGGTATCTACATGTGGGATTGCGATGGCAACGAGTTAAAAGCATGGAGAGGTATGAGGATGCCCAAGATTTTAGATCTTGCTGTAACACCAGATGGGGAAAATCTTATCAGCATTTTCTCTGATAAAGAAATTCGGATTTTGAATGTGGGAACAAATGCTGAGCGAGTTATCTCTGAAGACCATTCTATCACATCCCTCTCGATTTCTAGAGATAGTGAGTACTTTATCGTCAACCTAAACAGCCAGGAGATCCATATGTGGGATGTTGCTGGGAAACTGGAGAAGCCACTGAAGTATAAGGGTCACAGGCAGAGCAAATACGTAATACGCTCCTGCTTCGGTGGAGTGAATAGTAAATTTATTGCCAGCGGTAGTGAGAACTCACAGGTATGCTTGCTTCCCCCAATTTTGTTAATTTTCCTATACACGTGTATACCGATCATTCTTGGCTCTTGCACCACTAAACTAAATGGGTCCTCACAAGTTAGATAA

Protein Analysis

498

Amino Acids

55.76

Weight (kDa)

5.72

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 7 - 50 5.5e-22 WDR26 Lis-homology motif
WDR55 PF24796 195 - 331 1.2e-08 WDR55
Beta-prop_CAF1B_HIR1 PF24105 208 - 337 6.8e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 214 - 336 5.4e-21 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 214 - 380 5.8e-20 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 214 - 386 7.5e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd PF25047 215 - 347 1.5e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 219 - 466 1.6e-40 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 222 - 465 1.6e-30 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 222 - 381 5.4e-12 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 227 - 330 4.6e-13 Aladin seven-bladed propeller
WD40_Gbeta PF25391 237 - 334 4.2e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 266 - 327 6.4e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 273 - 437 8.9e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 275 - 447 1.4e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 336 - 464 4.4e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43920
fragaria_vesca FvH4_2g20200
malus_domestica MD10G1173700.v1.1
prunus_persica Prupe.8G212300_v2.0.a1
pyrus_communis pycom05g17030 pycom10g14980
rosa_chinensis RchiOBHm_Chr6g0285971
rosa_laevigata RLG00000012593
rosa_multiflora Rmu_sc0005513.1_g000002
rosa_roxburghii Rroxscaffold_7G00182560
rosa_rugosa Rorug06G0177800
rosa_samantha Rh6AG289900 Rh6BG291900 Rh6CG292900 Rh6DG285000
rosa_wichuraiana Rw6G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 455
AccB7I CCANNNNNTGG 1 cut(s) 747
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 1435
AciI CCGC 5 cut(s) 108, 186, 303, 618, 1371
AclWI GGATC 3 cut(s) 625, 633, 1246
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 3 cut(s) 481, 1131, 1358
AcuI CTGAAG 2 cut(s) 1197, 1313
AcvI CACGTG 1 cut(s) 1431
AfaI GTAC 4 cut(s) 648, 687, 901, 1223
AfiI CCNNNNNNNGG 3 cut(s) 747, 1001, 1261
AflIII ACRYGT 4 cut(s) 854, 1011, 1428, 1430
AgsI TTSAA 4 cut(s) 274, 297, 536, 1144
AjnI CCWGG 1 cut(s) 1245
AluBI AGCT 6 cut(s) 251, 463, 677, 716, 880, 942
AluI AGCT 6 cut(s) 251, 463, 677, 716, 880, 942
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 3 cut(s) 625, 633, 1246
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 5 cut(s) 29, 37, 105, 549, 738
ApoI RAATTY 3 cut(s) 481, 1131, 1358
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 29, 550, 1476
AsuHPI GGTGA 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 1476
BanII GRGCYC 1 cut(s) 833
BarI GAAGNNNNNNTAC 2 cut(s) 1383, 1415
BbrPI CACGTG 1 cut(s) 1431
BbsI GAAGAC 1 cut(s) 1185
BccI CCATC 6 cut(s) 85, 352, 421, 749, 1019, 1088
BceAI ACGGC 1 cut(s) 753
BciT130I CCWGG 1 cut(s) 1247
BciVI GTATCC 3 cut(s) 221, 404, 823
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 3 cut(s) 21, 266, 1209
BfuI GTATCC 3 cut(s) 221, 404, 823
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 1 cut(s) 108
BlpI GCTNAGC 1 cut(s) 1161
BlsI GCNGC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 1223
Bme1390I CCNGG 1 cut(s) 1247
Bme18I GGWCC 1 cut(s) 1476
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 29, 550, 1476
BmiI GGNNCC 3 cut(s) 797, 830, 1477
BmrFI CCNGG 1 cut(s) 1247
BmsI GCATC 2 cut(s) 153, 1050
BpiI GAAGAC 1 cut(s) 1185
BpmI CTGGAG 1 cut(s) 1301
Bpu1102I GCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 1 cut(s) 860
BsaAI YACGTR 3 cut(s) 855, 1324, 1431
BsaJI CCNNGG 1 cut(s) 509
Bsc4I CCNNNNNNNGG 3 cut(s) 747, 1001, 1261
Bse1I ACTGG 2 cut(s) 809, 1284
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 1 cut(s) 509
BseGI GGATG 5 cut(s) 313, 892, 1065, 1193, 1270
BseLI CCNNNNNNNGG 3 cut(s) 747, 1001, 1261
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 2 cut(s) 630, 1152
BseNI ACTGG 2 cut(s) 809, 1284
BseYI CCCAGC 1 cut(s) 1271
BshFI GGCC 5 cut(s) 31, 39, 107, 551, 740
BsiHKCI CYCGRG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 3 cut(s) 747, 1001, 1261
BsmAI GTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 683
BsnI GGCC 5 cut(s) 31, 39, 107, 551, 740
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 833
Bsp1407I TGTACA 2 cut(s) 646, 685
Bsp143I GATC 5 cut(s) 625, 630, 1075, 1251, 1440
Bsp1720I GCTNAGC 1 cut(s) 1161
BspACI CCGC 5 cut(s) 108, 186, 303, 618, 1371
BspANI GGCC 5 cut(s) 31, 39, 107, 551, 740
BspCNI CTCAG 2 cut(s) 631, 1153
BspLI GGNNCC 3 cut(s) 797, 830, 1477
BspPI GGATC 3 cut(s) 625, 633, 1246
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 135
BsrGI TGTACA 2 cut(s) 646, 685
BsrI ACTGG 2 cut(s) 809, 1284
BssECI CCNNGG 1 cut(s) 509
BssMI GATC 5 cut(s) 625, 630, 1075, 1251, 1440
BssNAI GTATAC 1 cut(s) 1436
BssT1I CCWWGG 1 cut(s) 509
Bst1107I GTATAC 1 cut(s) 1436
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 3 cut(s) 279, 392, 646
BstAPI GCANNNNNTGC 1 cut(s) 141
BstAUI TGTACA 2 cut(s) 646, 685
BstBAI YACGTR 3 cut(s) 855, 1324, 1431
BstC8I GCNNGC 4 cut(s) 142, 908, 1369, 1397
BstDEI CTNAG 3 cut(s) 118, 639, 1161
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 5 cut(s) 313, 892, 1065, 1193, 1270
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 5 cut(s) 628, 633, 1078, 1254, 1443
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 5 cut(s) 625, 630, 1075, 1251, 1440
BstMWI GCNNNNNNNGC 4 cut(s) 141, 453, 460, 548
BstNI CCWGG 1 cut(s) 1247
BstNSI RCATGY 2 cut(s) 906, 1015
BstSCI CCNGG 1 cut(s) 1245
BstSNI TACGTA 1 cut(s) 1324
BstV2I GAAGAC 1 cut(s) 1185
BstX2I RGATCY 3 cut(s) 630, 1075, 1251
BstYI RGATCY 3 cut(s) 630, 1075, 1251
BstZ17I GTATAC 1 cut(s) 1436
BsuI GTATCC 3 cut(s) 221, 404, 823
BsuRI GGCC 5 cut(s) 31, 39, 107, 551, 740
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 5 cut(s) 313, 892, 1065, 1193, 1270
BtsIMutI CAGTG 2 cut(s) 83, 1289
Cac8I GCNNGC 4 cut(s) 142, 908, 1369, 1397
CaiI CAGNNNCTG 1 cut(s) 166
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 29, 550, 1476
Csp6I GTAC 4 cut(s) 647, 686, 900, 1222
CviAII CATG 9 cut(s) 53, 114, 564, 825, 903, 926, 945, 1012, 1044
CviQI GTAC 4 cut(s) 647, 686, 900, 1222
DdeI CTNAG 3 cut(s) 118, 639, 1161
DpnI GATC 5 cut(s) 627, 632, 1077, 1253, 1442
DpnII GATC 5 cut(s) 625, 630, 1075, 1251, 1440
EaeI YGGCCR 1 cut(s) 738
Eco105I TACGTA 1 cut(s) 1324
Eco130I CCWWGG 1 cut(s) 509
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 1476
Eco57I CTGAAG 2 cut(s) 1197, 1313
Eco72I CACGTG 1 cut(s) 1431
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO109I RGGNCCY 1 cut(s) 1476
EcoRII CCWGG 1 cut(s) 1245
EcoT14I CCWWGG 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 1 cut(s) 509
FaeI CATG 9 cut(s) 56, 117, 567, 828, 906, 929, 948, 1015, 1047
FaqI GGGAC 1 cut(s) 683
FatI CATG 9 cut(s) 52, 113, 563, 824, 902, 925, 944, 1011, 1043
FauI CCCGC 2 cut(s) 179, 296
FauNDI CATATG 2 cut(s) 746, 1257
FblI GTMKAC 1 cut(s) 1435
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 5 cut(s) 320, 899, 1072, 1180, 1277
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 3 cut(s) 21, 266, 1209
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 1 cut(s) 455
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 1275
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 5 cut(s) 31, 39, 107, 551, 740
HhaI GCGC 1 cut(s) 456
Hin1II CATG 9 cut(s) 56, 117, 567, 828, 906, 929, 948, 1015, 1047
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 5 cut(s) 128, 170, 560, 839, 962
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 3 cut(s) 376, 1234, 1436
Hpy188I TCNGA 7 cut(s) 151, 162, 630, 991, 1123, 1137, 1177
Hpy188III TCNNGA 4 cut(s) 403, 959, 1201, 1209
Hpy8I GTNNAC 3 cut(s) 376, 1234, 1436
HpyAV CCTTC 3 cut(s) 97, 400, 745
HpyCH4III ACNGT 3 cut(s) 279, 392, 646
HpyCH4IV ACGT 3 cut(s) 854, 1323, 1430
HpyCH4V TGCA 6 cut(s) 334, 469, 605, 735, 906, 1458
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 453, 460, 548
HpyF3I CTNAG 3 cut(s) 118, 639, 1161
HpySE526I ACGT 3 cut(s) 854, 1323, 1430
Hsp92II CATG 9 cut(s) 56, 117, 567, 828, 906, 929, 948, 1015, 1047
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 5 cut(s) 625, 630, 1075, 1251, 1440
LmnI GCTCC 4 cut(s) 415, 795, 828, 1337
LweI GCATC 2 cut(s) 153, 1050
MaeI CTAG 3 cut(s) 21, 266, 1209
MaeII ACGT 3 cut(s) 854, 1323, 1430
MaeIII GTNAC 3 cut(s) 279, 1084, 1304
MalI GATC 5 cut(s) 627, 632, 1077, 1253, 1442
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 625, 630, 1075, 1251, 1440
MboII GAAGA 5 cut(s) 187, 251, 711, 773, 1190
MflI RGATCY 3 cut(s) 630, 1075, 1251
MhlI GDGCHC 1 cut(s) 833
MlyI GAGTC 3 cut(s) 137, 833, 956
MmeI TCCRAC 2 cut(s) 105, 501
MnlI CCTC 9 cut(s) 4, 29, 334, 501, 884, 1043, 1050, 1208, 1489
MseI TTAA 5 cut(s) 216, 225, 779, 1037, 1415
MslI CAYNNNNRTG 1 cut(s) 1390
MspA1I CMGCKG 1 cut(s) 1371
MspR9I CCNGG 1 cut(s) 1247
MvaI CCWGG 1 cut(s) 1247
MwoI GCNNNNNNNGC 4 cut(s) 141, 453, 460, 548
NdeI CATATG 2 cut(s) 746, 1257
NdeII GATC 5 cut(s) 625, 630, 1075, 1251, 1440
NlaIII CATG 9 cut(s) 56, 117, 567, 828, 906, 929, 948, 1015, 1047
NlaIV GGNNCC 3 cut(s) 797, 830, 1477
NmuCI GTSAC 2 cut(s) 279, 1304
NsbI TGCGCA 1 cut(s) 455
NspI RCATGY 2 cut(s) 906, 1015
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 1 cut(s) 1436
PfeI GAWTC 2 cut(s) 170, 560
PflMI CCANNNNNTGG 1 cut(s) 747
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 3 cut(s) 136, 833, 956
PmaCI CACGTG 1 cut(s) 1431
PmlI CACGTG 1 cut(s) 1431
PpsI GAGTC 3 cut(s) 136, 833, 956
Ppu21I YACGTR 3 cut(s) 855, 1324, 1431
PpuMI RGGWCCY 1 cut(s) 1476
PscI ACATGT 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 1476
Psp6I CCWGG 1 cut(s) 1245
PspCI CACGTG 1 cut(s) 1431
PspFI CCCAGC 1 cut(s) 1271
PspGI CCWGG 1 cut(s) 1245
PspN4I GGNNCC 3 cut(s) 797, 830, 1477
PspPI GGNCC 3 cut(s) 29, 550, 1476
PspPPI RGGWCCY 1 cut(s) 1476
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 3 cut(s) 630, 1075, 1251
RsaI GTAC 4 cut(s) 648, 687, 901, 1223
RsaNI GTAC 4 cut(s) 647, 686, 900, 1222
RseI CAYNNNNRTG 1 cut(s) 1390
SaqAI TTAA 5 cut(s) 216, 225, 779, 1037, 1415
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 5 cut(s) 625, 630, 1075, 1251, 1440
Sau96I GGNCC 3 cut(s) 29, 550, 1476
ScaI AGTACT 1 cut(s) 1223
SchI GAGTC 3 cut(s) 137, 833, 956
ScrFI CCNGG 1 cut(s) 1247
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 2 cut(s) 153, 1050
SinI GGWCC 1 cut(s) 1476
SmiMI CAYNNNNRTG 1 cut(s) 1390
SmlI CTYRAG 1 cut(s) 875
SmoI CTYRAG 1 cut(s) 875
SnaBI TACGTA 1 cut(s) 1324
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 5 cut(s) 108, 186, 303, 618, 1371
SspMI CTAG 3 cut(s) 21, 266, 1209
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 1245
StyI CCWWGG 1 cut(s) 509
TaaI ACNGT 3 cut(s) 279, 392, 646
TaiI ACGT 3 cut(s) 857, 1326, 1433
TaqI TCGA 5 cut(s) 5, 89, 173, 404, 1202
TatI WGTACW 3 cut(s) 646, 685, 1221
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 170, 560
Tru1I TTAA 5 cut(s) 216, 225, 779, 1037, 1415
Tru9I TTAA 5 cut(s) 216, 225, 779, 1037, 1415
TscAI CASTG 2 cut(s) 83, 1296
TseFI GTSAC 2 cut(s) 279, 1304
Tsp45I GTSAC 2 cut(s) 279, 1304
TspDTI ATGAA 4 cut(s) 102, 552, 711, 922
TspRI CASTG 2 cut(s) 83, 1296
Van91I CCANNNNNTGG 1 cut(s) 747
VpaK11BI GGWCC 1 cut(s) 1476
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 3 cut(s) 481, 1131, 1358
XbaI TCTAGA 1 cut(s) 1208
XceI RCATGY 2 cut(s) 906, 1015
XmiI GTMKAC 1 cut(s) 1435
XspI CTAG 3 cut(s) 21, 266, 1209
ZrmI AGTACT 1 cut(s) 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.