Rh6CG292900

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015082
Physical Location & Seq
Forward (+)
47066124 .. 47070549
4426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG292900.1

Sequence Viewer

Length: 1464 bp
ATGTCGATGGAGGGTTCGACTAGGCTCGGGCCAAGAGGCCTAATAAAGAAGCATGAGTTTGTAAGAATAATAATTCAGTGCCTATATTCGATGGGCTATGGGAGGGCTGCTTCATGCTTAGAGTTGGAGTCTGGCACTGCTTGCCAATCTGATTATTTTCAGATGCTGGAATCGAAGATATTGAGCGGGAATTGGAACGATTGTATTGATATCCTTAATGCCATTAAGGATTTGACAGAAGAAACAAGAACTACTGCTTTGTTTCTAGTATTCAAACAGTCACTTTTGGAGTATGCAAAGTGTGGGGATGACAAGATGGCTTTAGACATTTTGCAGAAAGAGGTTTCAGCATTACAGGTGGGCAGAGATAAGGTTCATAACCTGGCTCACAGTATCCTTTTCTCAAAGGATATGGAGCAGGGTAAGATGGACAACAATGAGGTTTGCGAATTGCGAAAAAAGCTGTTGCAAGAATTGGAAAATTTGCTTCCTCCACCAATTACACTACCTCCGCGCAGGTTGGAACATCTGGTTGAAACTGCTGTTTTGACCCAGATTGATTCATGTATGTATCATAATTTTTCGGATGCTGTTTCACTTTATGCAGACCATTTGTGTGGTAGGGATCAGATCCCTACTGAGACTGTACAGATTTTGACTGAGCATAAGGACGAAGTTTGGTTTGTACAATTTTCTCATAATGGGGAGTACTTAGCTTCTTCATCAAGTGATTGCACGGCCATCATATGGAAGGTGCTGGAAGACGGCAGCGTGATATTAAAACATACCCTATGGAGCCACCTTAAACCAGTATCCTTTGTGGCATGGAGCCCCGATGACTCAAAGTTGCTTACGTGTGGAAATGGTGAAGTCCTCAAGCTATGGGATGTGGAAACAGGTACATGCAAGCATACATTTGGAGATCATGGCTTCATTGTCAGCTCATGTGCTTGGTTTCCCGACTCAAGGCGACTTGTGTGTGGCAGTTCTGACCCTGAAAAAGGTATCTACATGTGGGATTGCGATGGCAACGAGTTAAAAGCATGGAGAGGTATGAGGATGCCCAAGATTTTAGATCTTGCTGTAACACCAGATGGGGAAAATCTTATCAGCATTTTCTCTGATAAAGAAATTCGGATTTTGAATGTGGGAACAAATACTGAGCGTGTTATCTCTGAAGACCATTCTATCACATCCCTCTCAATTTCTAGAGATAGTGAGTACTTTATCGTTAACCTAAATAGCCAGGAGATCCATATGTGGGATGTTGCTGGGAAACTGGAGAAGCCACTGAAGTATATGGGTCACAGGCAGAGCAAATACGTAATACGCTCCTGCTTCGGTGGAGTGAATAGTACATTTATTGCCAGCGGTAGTGAGAACTCACAGGTATGCTTGCTTCCCCCAATTTTGTTTGTTTTCCTATACATGTGTATACCAATCATTCTTGCACCACTAAACTGA

Protein Analysis

487

Amino Acids

54.92

Weight (kDa)

5.37

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 7 - 50 1e-20 WDR26 Lis-homology motif
Beta-prop_CAF1B_HIR1 PF24105 208 - 337 4e-12 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 214 - 386 3.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 214 - 336 1.6e-21 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 214 - 380 6.5e-21 CDC20/Fizzy WD40 domain
Beta-prop_Aladin PF25460 214 - 331 1.4e-12 Aladin seven-bladed propeller
WDR55 PF24796 214 - 331 1.4e-07 WDR55
Beta-prop_TEP1_2nd PF25047 214 - 346 5.7e-10 TEP-1 second beta-propeller
WD40 PF00400 215 - 250 1.4e-07 WD domain, G-beta repeat
Beta-prop_IFT122_1st PF23381 215 - 328 1e-06 IFT122 first beta-propeller
EIF3I PF24805 219 - 411 2.6e-06 EIF3I
Beta-prop_WDR5 PF25175 219 - 466 3.4e-40 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 221 - 381 1.3e-11 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 222 - 465 4.2e-30 THOC3 beta-propeller domain
WD40_Gbeta PF25391 230 - 343 7.2e-09 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 274 - 426 4.4e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 275 - 426 1.5e-10 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43920
fragaria_vesca FvH4_2g20200
malus_domestica MD10G1173700.v1.1
prunus_persica Prupe.8G212300_v2.0.a1
pyrus_communis pycom05g17030 pycom10g14980
rosa_chinensis RchiOBHm_Chr6g0285971
rosa_laevigata RLG00000012593
rosa_multiflora Rmu_sc0005513.1_g000002
rosa_roxburghii Rroxscaffold_7G00182560
rosa_rugosa Rorug06G0177800
rosa_samantha Rh6AG289900 Rh6BG291900 Rh6CG292900 Rh6DG285000
rosa_wichuraiana Rw6G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 507
AccB7I CCANNNNNTGG 1 cut(s) 747
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 1435
AccII CGCG 1 cut(s) 514
AciI CCGC 3 cut(s) 186, 512, 1371
AclWI GGATC 3 cut(s) 625, 633, 1246
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 2 cut(s) 481, 1131
AcuI CTGAAG 2 cut(s) 1197, 1313
AfaI GTAC 6 cut(s) 648, 687, 710, 901, 1223, 1357
AfiI CCNNNNNNNGG 4 cut(s) 747, 966, 1001, 1261
AflIII ACRYGT 3 cut(s) 854, 1011, 1428
AgsI TTSAA 3 cut(s) 274, 536, 1144
AjnI CCWGG 2 cut(s) 381, 1245
AluBI AGCT 4 cut(s) 463, 716, 880, 942
AluI AGCT 4 cut(s) 463, 716, 880, 942
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 3 cut(s) 625, 633, 1246
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 29, 37, 738
ApeKI GCWGC 2 cut(s) 107, 768
ApoI RAATTY 2 cut(s) 481, 1131
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 878
AvaI CYCGRG 1 cut(s) 26
BanII GRGCYC 1 cut(s) 833
BarI GAAGNNNNNNTAC 2 cut(s) 1383, 1415
BbsI GAAGAC 2 cut(s) 768, 1185
BbvI GCAGC 2 cut(s) 94, 780
BccI CCATC 6 cut(s) 85, 310, 421, 749, 1019, 1088
BceAI ACGGC 2 cut(s) 753, 781
BciT130I CCWGG 2 cut(s) 383, 1247
BciVI GTATCC 2 cut(s) 404, 823
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 3 cut(s) 21, 266, 1209
BfuAI ACCTGC 1 cut(s) 507
BfuI GTATCC 2 cut(s) 404, 823
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 2 cut(s) 108, 769
BlsI GCNGC 2 cut(s) 109, 770
BmcAI AGTACT 2 cut(s) 710, 1223
Bme1390I CCNGG 2 cut(s) 383, 1247
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 2 cut(s) 797, 830
BmrFI CCNGG 2 cut(s) 383, 1247
BmsI GCATC 3 cut(s) 153, 577, 1050
BpiI GAAGAC 2 cut(s) 768, 1185
BpmI CTGGAG 1 cut(s) 1301
BpuEI CTTGAG 2 cut(s) 860, 949
BsaAI YACGTR 2 cut(s) 855, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 4 cut(s) 747, 966, 1001, 1261
Bse1I ACTGG 2 cut(s) 809, 1284
BseBI CCWGG 2 cut(s) 383, 1247
BseGI GGATG 6 cut(s) 313, 592, 892, 1065, 1193, 1270
BseLI CCNNNNNNNGG 4 cut(s) 747, 966, 1001, 1261
BseMII CTCAG 3 cut(s) 630, 651, 1152
BseNI ACTGG 2 cut(s) 809, 1284
BseXI GCAGC 2 cut(s) 94, 780
BseYI CCCAGC 1 cut(s) 1271
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 3 cut(s) 31, 39, 740
BsiHKCI CYCGRG 1 cut(s) 26
BslI CCNNNNNNNGG 4 cut(s) 747, 966, 1001, 1261
BsmAI GTCTC 1 cut(s) 635
BsnI GGCC 3 cut(s) 31, 39, 740
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 833
Bsp1407I TGTACA 2 cut(s) 646, 685
Bsp143I GATC 5 cut(s) 625, 630, 922, 1075, 1251
BspACI CCGC 3 cut(s) 186, 512, 1371
BspANI GGCC 3 cut(s) 31, 39, 740
BspCNI CTCAG 3 cut(s) 631, 652, 1153
BspFNI CGCG 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 797, 830
BspMI ACCTGC 1 cut(s) 507
BspPI GGATC 3 cut(s) 625, 633, 1246
BsrBI CCGCTC 1 cut(s) 186
BsrGI TGTACA 2 cut(s) 646, 685
BsrI ACTGG 2 cut(s) 809, 1284
BssMI GATC 5 cut(s) 625, 630, 922, 1075, 1251
BssNAI GTATAC 1 cut(s) 1436
Bst1107I GTATAC 1 cut(s) 1436
Bst2UI CCWGG 2 cut(s) 383, 1247
Bst4CI ACNGT 3 cut(s) 279, 392, 646
BstAPI GCANNNNNTGC 1 cut(s) 141
BstAUI TGTACA 2 cut(s) 646, 685
BstBAI YACGTR 2 cut(s) 855, 1324
BstC8I GCNNGC 4 cut(s) 142, 908, 1369, 1397
BstDEI CTNAG 5 cut(s) 118, 639, 660, 712, 1161
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 6 cut(s) 313, 592, 892, 1065, 1193, 1270
BstFNI CGCG 1 cut(s) 514
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 5 cut(s) 628, 633, 925, 1078, 1254
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 5 cut(s) 625, 630, 922, 1075, 1251
BstMWI GCNNNNNNNGC 2 cut(s) 141, 460
BstNI CCWGG 2 cut(s) 383, 1247
BstNSI RCATGY 3 cut(s) 906, 1015, 1432
BstSCI CCNGG 2 cut(s) 381, 1245
BstSNI TACGTA 1 cut(s) 1324
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 2 cut(s) 94, 780
BstV2I GAAGAC 2 cut(s) 768, 1185
BstX2I RGATCY 3 cut(s) 630, 1075, 1251
BstXI CCANNNNNNTGG 1 cut(s) 617
BstYI RGATCY 3 cut(s) 630, 1075, 1251
BstZ17I GTATAC 1 cut(s) 1436
BsuI GTATCC 2 cut(s) 404, 823
BsuRI GGCC 3 cut(s) 31, 39, 740
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 313, 592, 892, 1065, 1193, 1270
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 3 cut(s) 83, 135, 1289
BveI ACCTGC 1 cut(s) 507
Cac8I GCNNGC 4 cut(s) 142, 908, 1369, 1397
CaiI CAGNNNCTG 1 cut(s) 166
CfoI GCGC 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 6 cut(s) 647, 686, 709, 900, 1222, 1356
CviQI GTAC 6 cut(s) 647, 686, 709, 900, 1222, 1356
DdeI CTNAG 5 cut(s) 118, 639, 660, 712, 1161
DpnI GATC 5 cut(s) 627, 632, 924, 1077, 1253
DpnII GATC 5 cut(s) 625, 630, 922, 1075, 1251
EaeI YGGCCR 1 cut(s) 738
Eco105I TACGTA 1 cut(s) 1324
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 833
Eco32I GATATC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 1197, 1313
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 381, 1245
EcoRV GATATC 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 833
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 2 cut(s) 746, 1257
FblI GTMKAC 1 cut(s) 1435
Fnu4HI GCNGC 2 cut(s) 108, 769
FokI GGATG 6 cut(s) 320, 599, 899, 1072, 1180, 1277
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 2 cut(s) 108, 769
FspBI CTAG 3 cut(s) 21, 266, 1209
GlaI GCGC 1 cut(s) 515
GluI GCNGC 2 cut(s) 108, 769
GsaI CCCAGC 1 cut(s) 1275
GsuI CTGGAG 1 cut(s) 1301
HaeIII GGCC 3 cut(s) 31, 39, 740
HhaI GCGC 1 cut(s) 516
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HinfI GANTC 5 cut(s) 128, 170, 560, 839, 962
HpaI GTTAAC 1 cut(s) 1234
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 2 cut(s) 1234, 1436
Hpy188I TCNGA 8 cut(s) 151, 162, 586, 630, 991, 1123, 1137, 1177
Hpy188III TCNNGA 2 cut(s) 959, 1209
Hpy8I GTNNAC 2 cut(s) 1234, 1436
HpyAV CCTTC 1 cut(s) 745
HpyCH4III ACNGT 3 cut(s) 279, 392, 646
HpyCH4IV ACGT 2 cut(s) 854, 1323
HpyCH4V TGCA 7 cut(s) 296, 334, 469, 605, 735, 906, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 460
HpyF3I CTNAG 5 cut(s) 118, 639, 660, 712, 1161
HpySE526I ACGT 2 cut(s) 854, 1323
HspAI GCGC 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 1234
Kzo9I GATC 5 cut(s) 625, 630, 922, 1075, 1251
LmnI GCTCC 4 cut(s) 415, 795, 828, 1337
Lsp1109I GCAGC 2 cut(s) 94, 780
LweI GCATC 3 cut(s) 153, 577, 1050
MaeI CTAG 3 cut(s) 21, 266, 1209
MaeII ACGT 2 cut(s) 854, 1323
MaeIII GTNAC 3 cut(s) 279, 1084, 1304
MalI GATC 5 cut(s) 627, 632, 924, 1077, 1253
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 625, 630, 922, 1075, 1251
MboII GAAGA 5 cut(s) 187, 251, 711, 773, 1190
MflI RGATCY 3 cut(s) 630, 1075, 1251
MhlI GDGCHC 1 cut(s) 833
MlyI GAGTC 3 cut(s) 137, 833, 956
MmeI TCCRAC 2 cut(s) 105, 501
MseI TTAA 6 cut(s) 216, 225, 779, 804, 1037, 1233
MslI CAYNNNNRTG 2 cut(s) 615, 1390
MspA1I CMGCKG 1 cut(s) 1371
MspR9I CCNGG 2 cut(s) 383, 1247
MvaI CCWGG 2 cut(s) 383, 1247
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 2 cut(s) 141, 460
NdeI CATATG 2 cut(s) 746, 1257
NdeII GATC 5 cut(s) 625, 630, 922, 1075, 1251
NlaIV GGNNCC 2 cut(s) 797, 830
NmuCI GTSAC 2 cut(s) 279, 1304
NspI RCATGY 3 cut(s) 906, 1015, 1432
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 2 cut(s) 1011, 1428
PfeI GAWTC 2 cut(s) 170, 560
PflMI CCANNNNNTGG 1 cut(s) 747
PkrI GCNGC 2 cut(s) 109, 770
PleI GAGTC 3 cut(s) 136, 833, 956
PpsI GAGTC 3 cut(s) 136, 833, 956
Ppu21I YACGTR 2 cut(s) 855, 1324
PscI ACATGT 2 cut(s) 1011, 1428
Psp6I CCWGG 2 cut(s) 381, 1245
PspFI CCCAGC 1 cut(s) 1271
PspGI CCWGG 2 cut(s) 381, 1245
PspN4I GGNNCC 2 cut(s) 797, 830
PspPI GGNCC 1 cut(s) 29
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 3 cut(s) 630, 1075, 1251
RsaI GTAC 6 cut(s) 648, 687, 710, 901, 1223, 1357
RsaNI GTAC 6 cut(s) 647, 686, 709, 900, 1222, 1356
RseI CAYNNNNRTG 2 cut(s) 615, 1390
SaqAI TTAA 6 cut(s) 216, 225, 779, 804, 1037, 1233
SatI GCNGC 2 cut(s) 108, 769
Sau3AI GATC 5 cut(s) 625, 630, 922, 1075, 1251
Sau96I GGNCC 1 cut(s) 29
ScaI AGTACT 2 cut(s) 710, 1223
SchI GAGTC 3 cut(s) 137, 833, 956
ScrFI CCNGG 2 cut(s) 383, 1247
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 3 cut(s) 153, 577, 1050
SmiMI CAYNNNNRTG 2 cut(s) 615, 1390
SmlI CTYRAG 2 cut(s) 875, 964
SmoI CTYRAG 2 cut(s) 875, 964
SnaBI TACGTA 1 cut(s) 1324
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 186, 512, 1371
SspMI CTAG 3 cut(s) 21, 266, 1209
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 381, 1245
TaaI ACNGT 3 cut(s) 279, 392, 646
TaiI ACGT 2 cut(s) 857, 1326
TaqI TCGA 4 cut(s) 5, 17, 89, 173
TatI WGTACW 5 cut(s) 646, 685, 708, 1221, 1355
TfiI GAWTC 2 cut(s) 170, 560
Tru1I TTAA 6 cut(s) 216, 225, 779, 804, 1037, 1233
Tru9I TTAA 6 cut(s) 216, 225, 779, 804, 1037, 1233
TscAI CASTG 3 cut(s) 83, 142, 1296
TseFI GTSAC 2 cut(s) 279, 1304
TseI GCWGC 2 cut(s) 107, 768
Tsp45I GTSAC 2 cut(s) 279, 1304
TspDTI ATGAA 5 cut(s) 102, 365, 552, 711, 922
TspRI CASTG 3 cut(s) 83, 142, 1296
Van91I CCANNNNNTGG 1 cut(s) 747
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 2 cut(s) 481, 1131
XbaI TCTAGA 1 cut(s) 1208
XceI RCATGY 3 cut(s) 906, 1015, 1432
XmiI GTMKAC 1 cut(s) 1435
XspI CTAG 3 cut(s) 21, 266, 1209
ZrmI AGTACT 2 cut(s) 710, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.