pycom01g00110

Cell wall-associated hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019640
Physical Location & Seq
Forward (+)
312433 .. 314603
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1533 bp
ATGTCTGAAATGGGTTCACTCCTGCTCCCCAGTGCACTCTTATATTTAGGCACTTTACGTTTAAATTTATTCACATTTTCCACATCACTACACTTTATGGCTTTGTCACCCTCCGAGTGTCGGCCAATACAACTTGATTCAGAGTCCAAGTGGACATTCCGGGTCGGGGTTTCTATAAACTACTTTAAAATCCGGACTAAATACACTTTGATTTCTATAGAGTTTAAATATGAATTTGTAAACTCTTTTAAGCTTTTAATTGAGTACGTCTCCTTAATTCGTTGGTATAAATATAAGGGCGTTTCTAAGATGCGGACATTAACAACGCACCTTGCGTGCGGACCGCTATCGCACGGTGGTGATTTGTTGAGCTCACGAGTAGATTTGAGTTGTTGTATTTGTCGGAGAGAAAGTAGGAGGCAGAGAGCTGAGGGATATGTTCGAGCATTGAAGAGGGAAAGAAAAAAAGCTTATACCTTTAACTTTTTTGGTTCACAAGGGTGTTTCTGTAGGCAAAAGCGAGGGAGATTAGGGTTGTCTTTGACGGGAAAGAGAGGTAGAGAGAGGGAGTTGGTGAAGGTTGGGAGAGAGTTGTCTCGCGCCCCTAGGTATTCTCTACCTACCCACCTGTGTTGGTTTCTGGTATATAGGTACCTTTTTGTTGAAGGTCATTCAAGCTTTTCCTGGGAGTATGGAATGAGTTACTTCAGTGCCGTGGTGCCTCGTAGTCGAACATTGGCTCGAGGCATTTTCTCTACCCCTTCTTACTGTGAAAAATTAGGGCATTATATTAGTAAGTTTATTTTATATATGTATTTATATACACACATTATAATTTTTGCTCGGATCTTAGTATTTATACCGAAAAAATATTCGTTTCATCTAAAGCCACCTCCTGTATGTGGAAGCCTTACCAGTTACCACCATATTGTAGATGTTAATAGATATTTCTATATATTGACACCTACTCCATGCAATCTCTACAAAATCATATCCATAAGTTGCCAAGTCCAACTCTACTTAGGTTTAGAACCTTACACATTATTATTAGCTTGCTGCTACAGCATGATTGAATCAAATAAAAGCCGTCAGAGGAATCAACAGCTCTTACTTACCCGCATGCATATTTGTTTGAATTGTCAAGCTTTCATTCCTCACTTCATCATTTACACACCCCACCTCCTCTATCTGCCGCCTTTCAATCCTATAAATAGAAGGCCGCACTTCATTCTTTCTCTCTCTCTCTCTATATATATATATATAGAGAGAGAGAGAGAGAGAGAGGAAAGAAGGAGAGCTAGGGTGAGTGGTTTATGTAATGTGTTTCAAAATTTTCTTATTCCTGATAAAACGAAAACCATAAATTATCTTTGTGTAAATGTTTATGATGCGATATATATACAATCTATGTGGAATTACTTGAGATTGTTCATGGAGCATGAGAACTTTAGTGTTGCATTGATTCAAGGGATAAATGTAAACTGCACAGGGGGCAGAATGACCGTTAGGAGTAACGTGGCGAGTGACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

511

Amino Acids

59.77

Weight (kDa)

9.72

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019640)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07775
pyrus_communis pycom01g00110
rosa_laevigata RLG00000000161 RLG00000000418
rosa_multiflora Rmu_sc0013945.1_g000007
rosa_roxburghii Rroxscaffold_2G00118900 Rroxscaffold_2G00118910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
Acc65I GGTACC 1 cut(s) 651
AccB1I GGYRCC 2 cut(s) 651, 718
AccII CGCG 1 cut(s) 600
AccIII TCCGGA 1 cut(s) 192
AciI CCGC 6 cut(s) 313, 339, 344, 1117, 1193, 1220
AclWI GGATC 1 cut(s) 854
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 3 cut(s) 64, 233, 1330
AcuI CTGAAG 1 cut(s) 691
AfaI GTAC 2 cut(s) 266, 653
AfiI CCNNNNNNNGG 3 cut(s) 120, 166, 902
AgsI TTSAA 8 cut(s) 451, 665, 675, 1073, 1135, 1201, 1328, 1466
AjnI CCWGG 1 cut(s) 683
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AleI CACNNNNGTG 2 cut(s) 357, 499
AluBI AGCT 9 cut(s) 253, 372, 428, 470, 678, 1052, 1105, 1145, 1298
AluI AGCT 9 cut(s) 253, 372, 428, 470, 678, 1052, 1105, 1145, 1298
Alw21I GWGCWC 2 cut(s) 37, 374
Alw26I GTCTC 2 cut(s) 274, 600
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 1 cut(s) 854
Ama87I CYCGRG 1 cut(s) 741
Aor13HI TCCGGA 1 cut(s) 192
AoxI GGCC 2 cut(s) 122, 1217
ApaLI GTGCAC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 1056
ApoI RAATTY 3 cut(s) 64, 233, 1330
Asp718I GGTACC 1 cut(s) 651
AspA2I CCTAGG 1 cut(s) 605
AspLEI GCGC 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 4 cut(s) 99, 371, 586, 1315
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 1 cut(s) 341
AvrII CCTAGG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 37
BanI GGYRCC 2 cut(s) 651, 718
BanII GRGCYC 1 cut(s) 374
BauI CACGAG 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 37, 374
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 1043
BceAI ACGGC 2 cut(s) 698, 1071
BciT130I CCWGG 1 cut(s) 685
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 274, 600
BfaI CTAG 2 cut(s) 606, 1299
BfmI CTRYAG 3 cut(s) 216, 508, 1060
BisI GCNGC 3 cut(s) 1057, 1193, 1220
BlnI CCTAGG 1 cut(s) 605
BlsI GCNGC 3 cut(s) 1058, 1194, 1221
Bme1390I CCNGG 2 cut(s) 161, 685
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 653, 720
BmrFI CCNGG 2 cut(s) 161, 685
BmrI ACTGGG 1 cut(s) 24
BmsI GCATC 2 cut(s) 300, 1378
BmuI ACTGGG 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 254, 286
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 1441
BpuMI CCSGG 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 605, 684, 714
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 8 cut(s) 9, 39, 397, 427, 952, 982, 1164, 1194
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 166, 902
Bse1I ACTGG 2 cut(s) 30, 915
BseAI TCCGGA 1 cut(s) 192
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 3 cut(s) 605, 684, 714
BseLI CCNNNNNNNGG 3 cut(s) 120, 166, 902
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 30, 915
BseRI GAGGAG 1 cut(s) 1172
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 1 cut(s) 1043
BsgI GTGCAG 1 cut(s) 1468
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 2 cut(s) 124, 1219
BshNI GGYRCC 2 cut(s) 651, 718
BsiHKAI GWGCWC 2 cut(s) 37, 374
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 2 cut(s) 160, 193
BslI CCNNNNNNNGG 3 cut(s) 120, 166, 902
BsmAI GTCTC 2 cut(s) 274, 600
BsmBI CGTCTC 1 cut(s) 274
BsnI GGCC 2 cut(s) 124, 1219
BsoBI CYCGRG 1 cut(s) 741
Bsp1286I GDGCHC 2 cut(s) 37, 374
Bsp13I TCCGGA 1 cut(s) 192
Bsp143I GATC 1 cut(s) 846
BspACI CCGC 6 cut(s) 313, 339, 344, 1117, 1193, 1220
BspANI GGCC 2 cut(s) 124, 1219
BspCNI CTCAG 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 192
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 653, 720
BspPI GGATC 1 cut(s) 854
BspT107I GGYRCC 2 cut(s) 651, 718
BsrI ACTGG 2 cut(s) 30, 915
BssECI CCNNGG 3 cut(s) 605, 684, 714
BssMI GATC 1 cut(s) 846
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 605
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 3 cut(s) 356, 770, 1504
Bst6I CTCTTC 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 337, 1054, 1121
BstDEI CTNAG 4 cut(s) 306, 429, 850, 1021
BstDSI CCRYGG 1 cut(s) 714
BstFNI CGCG 1 cut(s) 600
BstHHI GCGC 1 cut(s) 602
BstKTI GATC 1 cut(s) 849
BstMAI GTCTC 2 cut(s) 274, 600
BstMBI GATC 1 cut(s) 846
BstMWI GCNNNNNNNGC 2 cut(s) 1062, 1491
BstNI CCWGG 1 cut(s) 685
BstNSI RCATGY 1 cut(s) 1123
BstSCI CCNGG 2 cut(s) 159, 683
BstSFI CTRYAG 3 cut(s) 216, 508, 1060
BstSLI GKGCMC 1 cut(s) 37
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 1 cut(s) 1043
BstX2I RGATCY 1 cut(s) 846
BstYI RGATCY 1 cut(s) 846
BsuRI GGCC 2 cut(s) 124, 1219
BtgI CCRYGG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 37, 715
Cac8I GCNNGC 3 cut(s) 337, 1054, 1121
CfoI GCGC 1 cut(s) 602
Cfr13I GGNCC 1 cut(s) 341
CpoI CGGWCCG 1 cut(s) 341
Csp6I GTAC 2 cut(s) 265, 652
CspCI CAANNNNNGTGG 4 cut(s) 614, 649, 1391, 1426
CspI CGGWCCG 1 cut(s) 341
CviAII CATG 5 cut(s) 972, 1066, 1120, 1432, 1439
CviQI GTAC 2 cut(s) 265, 652
DdeI CTNAG 4 cut(s) 306, 429, 850, 1021
DpnI GATC 1 cut(s) 848
DpnII GATC 1 cut(s) 846
DraI TTTAAA 3 cut(s) 63, 187, 226
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 446
Ecl136II GAGCTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 605
Eco24I GRGCYC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 691
Eco88I CYCGRG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 605
EcoT22I ATGCAT 1 cut(s) 1125
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 605
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 5 cut(s) 975, 1069, 1123, 1435, 1442
FatI CATG 5 cut(s) 971, 1065, 1119, 1431, 1438
FauI CCCGC 1 cut(s) 1124
Fnu4HI GCNGC 3 cut(s) 1057, 1193, 1220
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 3 cut(s) 1057, 1193, 1220
FspBI CTAG 2 cut(s) 606, 1299
GlaI GCGC 1 cut(s) 601
GluI GCNGC 3 cut(s) 1057, 1193, 1220
HaeIII GGCC 2 cut(s) 124, 1219
HapII CCGG 2 cut(s) 160, 193
HhaI GCGC 1 cut(s) 602
Hin1II CATG 5 cut(s) 975, 1069, 1123, 1435, 1442
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HindIII AAGCTT 4 cut(s) 251, 468, 676, 1143
HinfI GANTC 5 cut(s) 137, 143, 1073, 1096, 1462
HpaII CCGG 2 cut(s) 160, 193
HphI GGTGA 4 cut(s) 99, 371, 586, 1315
Hpy166II GTNNAC 6 cut(s) 17, 35, 153, 241, 494, 1480
Hpy188I TCNGA 6 cut(s) 7, 115, 142, 405, 846, 1092
Hpy188III TCNNGA 3 cut(s) 193, 375, 1343
Hpy8I GTNNAC 6 cut(s) 17, 35, 153, 241, 494, 1480
HpyAV CCTTC 5 cut(s) 571, 659, 771, 1209, 1284
HpyCH4III ACNGT 3 cut(s) 356, 770, 1504
HpyCH4IV ACGT 3 cut(s) 58, 267, 1515
HpyCH4V TGCA 5 cut(s) 35, 975, 1123, 1457, 1485
HpyF10VI GCNNNNNNNGC 2 cut(s) 1062, 1491
HpyF3I CTNAG 4 cut(s) 306, 429, 850, 1021
HpySE526I ACGT 3 cut(s) 58, 267, 1515
Hsp92II CATG 5 cut(s) 975, 1069, 1123, 1435, 1442
HspAI GCGC 1 cut(s) 600
Kpn2I TCCGGA 1 cut(s) 192
KpnI GGTACC 1 cut(s) 655
Kzo9I GATC 1 cut(s) 846
LmnI GCTCC 2 cut(s) 30, 1435
Lsp1109I GCAGC 1 cut(s) 1043
LweI GCATC 2 cut(s) 300, 1378
MaeI CTAG 2 cut(s) 606, 1299
MaeII ACGT 3 cut(s) 58, 267, 1515
MaeIII GTNAC 5 cut(s) 105, 701, 917, 1511, 1523
MalI GATC 1 cut(s) 848
MboI GATC 1 cut(s) 846
MboII GAAGA 1 cut(s) 463
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 2 cut(s) 37, 374
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 383, 1036
Mph1103I ATGCAT 1 cut(s) 1125
MroI TCCGGA 1 cut(s) 192
MseI TTAA 9 cut(s) 62, 186, 225, 249, 257, 275, 320, 480, 939
MslI CAYNNNNRTG 2 cut(s) 357, 499
MspI CCGG 2 cut(s) 160, 193
MspR9I CCNGG 2 cut(s) 161, 685
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 2 cut(s) 1062, 1491
NciI CCSGG 1 cut(s) 161
NdeII GATC 1 cut(s) 846
NlaIII CATG 5 cut(s) 975, 1069, 1123, 1435, 1442
NlaIV GGNNCC 2 cut(s) 653, 720
NmuCI GTSAC 2 cut(s) 105, 1523
NsiI ATGCAT 1 cut(s) 1125
NspI RCATGY 1 cut(s) 1123
OliI CACNNNNGTG 2 cut(s) 357, 499
PaeI GCATGC 1 cut(s) 1123
PaeR7I CTCGAG 1 cut(s) 741
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PfeI GAWTC 4 cut(s) 137, 1073, 1096, 1462
PkrI GCNGC 3 cut(s) 1058, 1194, 1221
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PsiI TTATAA 1 cut(s) 833
Psp124BI GAGCTC 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 683
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 2 cut(s) 653, 720
PspPI GGNCC 1 cut(s) 341
PspXI VCTCGAGB 1 cut(s) 741
PsuI RGATCY 1 cut(s) 846
RsaI GTAC 2 cut(s) 266, 653
RsaNI GTAC 2 cut(s) 265, 652
RseI CAYNNNNRTG 2 cut(s) 357, 499
Rsr2I CGGWCCG 1 cut(s) 341
RsrII CGGWCCG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 374
SaqAI TTAA 9 cut(s) 62, 186, 225, 249, 257, 275, 320, 480, 939
SatI GCNGC 3 cut(s) 1057, 1193, 1220
Sau3AI GATC 1 cut(s) 846
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 2 cut(s) 161, 685
SduI GDGCHC 2 cut(s) 37, 374
SfaNI GCATC 2 cut(s) 300, 1378
SfcI CTRYAG 3 cut(s) 216, 508, 1060
Sfr274I CTCGAG 1 cut(s) 741
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 357, 499
SmlI CTYRAG 2 cut(s) 741, 1420
SmoI CTYRAG 2 cut(s) 741, 1420
SphI GCATGC 1 cut(s) 1123
SsiI CCGC 6 cut(s) 313, 339, 344, 1117, 1193, 1220
SspI AATATT 1 cut(s) 872
SspMI CTAG 2 cut(s) 606, 1299
SstI GAGCTC 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 159, 683
StyI CCWWGG 1 cut(s) 605
TaaI ACNGT 3 cut(s) 356, 770, 1504
TaiI ACGT 3 cut(s) 61, 270, 1518
TaqI TCGA 3 cut(s) 442, 730, 742
TauI GCSGC 2 cut(s) 1195, 1222
TfiI GAWTC 4 cut(s) 137, 1073, 1096, 1462
Tru1I TTAA 9 cut(s) 62, 186, 225, 249, 257, 275, 320, 480, 939
Tru9I TTAA 9 cut(s) 62, 186, 225, 249, 257, 275, 320, 480, 939
TscAI CASTG 2 cut(s) 37, 715
TseFI GTSAC 2 cut(s) 105, 1523
TseI GCWGC 1 cut(s) 1056
Tsp45I GTSAC 2 cut(s) 105, 1523
TspDTI ATGAA 6 cut(s) 246, 869, 1138, 1150, 1216, 1420
TspRI CASTG 2 cut(s) 37, 715
VneI GTGCAC 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 3 cut(s) 64, 233, 1330
XceI RCATGY 1 cut(s) 1123
XhoI CTCGAG 1 cut(s) 741
XmaJI CCTAGG 1 cut(s) 605
XspI CTAG 2 cut(s) 606, 1299
Zsp2I ATGCAT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.