Rmu_sc0013945.1_g000007

Cell wall-associated hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013945.1
OG0019640
Physical Location & Seq
Reverse (-)
22675 .. 23505
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013945.1_g000007.1.cds

Sequence Viewer

Length: 552 bp
atgtctatttcacggagcctctctccgagacagtgcccagatcgttacgcctttcgtgcgggtcggaacttacccgtcaaggaatttcgctaccttaggaccgttatagttacggtcgccgttcaccggggcttcggtcgccggctcccctgtcatcaggtcaccaacttccttgaccttccggccctgggcaggcgtcagcccccatacatggtcttacgactttgcggagccctgtgtttttggtacccttttgttgaaggtcgttcgagcttttcctgggagtatggcatgggttacttcagcgccgtggcgcctggtactcgaacattggctcggggcattttctctaccccttcttaccctgaaaaatcaggggcacctcgcgccctgactcaccctccgtggacgaaccttgcggaggaacccttaggttttcagggcattggattctcaccaatgtttgcgttactcaagtcgacatttcgcttccgcttcgtccacccccactcgcgcggttgcttttctctaggcggaacgctcccctattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.7

Weight (kDa)

10.53

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019640)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07775
pyrus_communis pycom01g00110
rosa_laevigata RLG00000000161 RLG00000000418
rosa_multiflora Rmu_sc0013945.1_g000007
rosa_roxburghii Rroxscaffold_2G00118900 Rroxscaffold_2G00118910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 246
AccB1I GGYRCC 3 cut(s) 246, 313, 379
AccI GTMKAC 1 cut(s) 479
AccII CGCG 3 cut(s) 387, 514, 516
AciI CCGC 6 cut(s) 59, 228, 419, 493, 516, 534
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 286
AcyI GRCGYC 2 cut(s) 196, 314
AfaI GTAC 2 cut(s) 248, 322
AfiI CCNNNNNNNGG 5 cut(s) 126, 188, 192, 211, 421
AgsI TTSAA 1 cut(s) 260
AjnI CCWGG 3 cut(s) 186, 278, 316
AluBI AGCT 1 cut(s) 273
AluI AGCT 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 1 cut(s) 183
ApoI RAATTY 1 cut(s) 83
Asp718I GGTACC 1 cut(s) 246
AspLEI GCGC 4 cut(s) 308, 316, 389, 516
AspS9I GGNCC 2 cut(s) 99, 184
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 4 cut(s) 116, 154, 389, 447
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 1 cut(s) 99
AxyI CCTNAGG 2 cut(s) 95, 430
BaeGI GKGCMC 2 cut(s) 38, 382
BanI GGYRCC 3 cut(s) 246, 313, 379
BanII GRGCYC 1 cut(s) 235
BceAI ACGGC 2 cut(s) 104, 293
BciT130I CCWGG 3 cut(s) 188, 280, 318
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 1 cut(s) 530
BfoI RGCGCY 2 cut(s) 309, 317
Bme1390I CCNGG 4 cut(s) 128, 188, 280, 318
Bme18I GGWCC 1 cut(s) 99
BmeT110I CYCGRG 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 99, 184
BmiI GGNNCC 7 cut(s) 17, 146, 232, 248, 315, 381, 426
BmrFI CCNGG 4 cut(s) 128, 188, 280, 318
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 196, 314
BsaJI CCNNGG 6 cut(s) 127, 186, 187, 279, 309, 404
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 188, 192, 211, 421
Bse118I RCCGGY 1 cut(s) 141
Bse21I CCTNAGG 2 cut(s) 95, 430
BseBI CCWGG 3 cut(s) 188, 280, 318
BseDI CCNNGG 6 cut(s) 127, 186, 187, 279, 309, 404
BseLI CCNNNNNNNGG 5 cut(s) 126, 188, 192, 211, 421
BseSI GKGCMC 2 cut(s) 38, 382
Bsh1236I CGCG 3 cut(s) 387, 514, 516
Bsh1285I CGRYCG 2 cut(s) 117, 139
BshFI GGCC 1 cut(s) 185
BshNI GGYRCC 3 cut(s) 246, 313, 379
BsiEI CGRYCG 2 cut(s) 117, 139
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 3 cut(s) 127, 142, 182
BslI CCNNNNNNNGG 5 cut(s) 126, 188, 192, 211, 421
BsmAI GTCTC 1 cut(s) 22
BsnI GGCC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 3 cut(s) 38, 235, 382
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 6 cut(s) 59, 228, 419, 493, 516, 534
BspANI GGCC 1 cut(s) 185
BspFNI CGCG 3 cut(s) 387, 514, 516
BspLI GGNNCC 7 cut(s) 17, 146, 232, 248, 315, 381, 426
BspT107I GGYRCC 3 cut(s) 246, 313, 379
BsrFI RCCGGY 1 cut(s) 141
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 6 cut(s) 127, 186, 187, 279, 309, 404
BssMI GATC 1 cut(s) 40
BssNI GRCGYC 2 cut(s) 196, 314
Bst2UI CCWGG 3 cut(s) 188, 280, 318
Bst4CI ACNGT 3 cut(s) 33, 103, 115
BstACI GRCGYC 2 cut(s) 196, 314
BstC8I GCNNGC 2 cut(s) 143, 194
BstDEI CTNAG 2 cut(s) 95, 430
BstDSI CCRYGG 2 cut(s) 309, 404
BstEII GGTNACC 1 cut(s) 160
BstENI CCTNNNNNAGG 1 cut(s) 419
BstFNI CGCG 3 cut(s) 387, 514, 516
BstH2I RGCGCY 2 cut(s) 309, 317
BstHHI GCGC 4 cut(s) 308, 316, 389, 516
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 1 cut(s) 40
BstMCI CGRYCG 2 cut(s) 117, 139
BstMWI GCNNNNNNNGC 3 cut(s) 56, 138, 386
BstNI CCWGG 3 cut(s) 188, 280, 318
BstPI GGTNACC 1 cut(s) 160
BstSCI CCNGG 4 cut(s) 126, 186, 278, 316
BstSLI GKGCMC 2 cut(s) 38, 382
BstUI CGCG 3 cut(s) 387, 514, 516
Bsu36I CCTNAGG 2 cut(s) 95, 430
BsuRI GGCC 1 cut(s) 185
BtgI CCRYGG 2 cut(s) 309, 404
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 143, 194
CfoI GCGC 4 cut(s) 308, 316, 389, 516
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 99, 184
CseI GACGC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 247, 321
CviAII CATG 2 cut(s) 211, 292
CviJI RGCY 8 cut(s) 18, 132, 145, 185, 202, 233, 273, 335
CviKI_1 RGCY 8 cut(s) 18, 132, 145, 185, 202, 233, 273, 335
CviQI GTAC 2 cut(s) 247, 321
DdeI CTNAG 2 cut(s) 95, 430
DinI GGCGCC 1 cut(s) 315
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 549
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 286
Eco81I CCTNAGG 2 cut(s) 95, 430
Eco88I CYCGRG 1 cut(s) 336
Eco91I GGTNACC 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 160
EcoRII CCWGG 3 cut(s) 186, 278, 316
EcoT38I GRGCYC 1 cut(s) 235
EgeI GGCGCC 1 cut(s) 315
EheI GGCGCC 1 cut(s) 315
FaeI CATG 2 cut(s) 214, 295
FaiI YATR 5 cut(s) 107, 208, 212, 288, 293
FatI CATG 2 cut(s) 210, 291
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 235
FspBI CTAG 1 cut(s) 530
GlaI GCGC 4 cut(s) 307, 315, 388, 515
HaeII RGCGCY 2 cut(s) 309, 317
HaeIII GGCC 1 cut(s) 185
HapII CCGG 3 cut(s) 127, 142, 182
HgaI GACGC 1 cut(s) 185
HhaI GCGC 4 cut(s) 308, 316, 389, 516
Hin1I GRCGYC 2 cut(s) 196, 314
Hin1II CATG 2 cut(s) 214, 295
Hin6I GCGC 4 cut(s) 306, 314, 387, 514
HinP1I GCGC 4 cut(s) 306, 314, 387, 514
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 2 cut(s) 394, 450
HpaII CCGG 3 cut(s) 127, 142, 182
HphI GGTGA 4 cut(s) 116, 154, 389, 447
Hpy166II GTNNAC 4 cut(s) 124, 408, 480, 502
Hpy188I TCNGA 2 cut(s) 27, 66
Hpy8I GTNNAC 4 cut(s) 124, 408, 480, 502
HpyAV CCTTC 3 cut(s) 188, 254, 366
HpyCH4III ACNGT 3 cut(s) 33, 103, 115
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 138, 386
HpyF3I CTNAG 2 cut(s) 95, 430
Hsp92I GRCGYC 2 cut(s) 196, 314
Hsp92II CATG 2 cut(s) 214, 295
HspAI GCGC 4 cut(s) 306, 314, 387, 514
KasI GGCGCC 1 cut(s) 313
KpnI GGTACC 1 cut(s) 250
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 4 cut(s) 15, 150, 230, 546
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 5 cut(s) 44, 109, 160, 296, 468
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MhlI GDGCHC 3 cut(s) 38, 235, 382
MluCI AATT 1 cut(s) 83
Mly113I GGCGCC 1 cut(s) 314
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 4 cut(s) 29, 393, 411, 415
MroNI GCCGGC 1 cut(s) 141
MspI CCGG 3 cut(s) 127, 142, 182
MspR9I CCNGG 4 cut(s) 128, 188, 280, 318
MvaI CCWGG 3 cut(s) 188, 280, 318
MvnI CGCG 3 cut(s) 387, 514, 516
MwoI GCNNNNNNNGC 3 cut(s) 56, 138, 386
NaeI GCCGGC 1 cut(s) 143
NarI GGCGCC 1 cut(s) 314
NciI CCSGG 1 cut(s) 128
NdeII GATC 1 cut(s) 40
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 2 cut(s) 214, 295
NlaIV GGNNCC 7 cut(s) 17, 146, 232, 248, 315, 381, 426
NmuCI GTSAC 1 cut(s) 160
PasI CCCWGGG 1 cut(s) 187
PdiI GCCGGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 450
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 186, 278, 316
PspEI GGTNACC 1 cut(s) 160
PspGI CCWGG 3 cut(s) 186, 278, 316
PspN4I GGNNCC 7 cut(s) 17, 146, 232, 248, 315, 381, 426
PspPI GGNCC 2 cut(s) 99, 184
RsaI GTAC 2 cut(s) 248, 322
RsaNI GTAC 2 cut(s) 247, 321
SalI GTCGAC 1 cut(s) 478
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 2 cut(s) 99, 184
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 4 cut(s) 128, 188, 280, 318
SduI GDGCHC 3 cut(s) 38, 235, 382
SetI ASST 8 cut(s) 96, 162, 180, 265, 275, 385, 417, 436
SfoI GGCGCC 1 cut(s) 315
SinI GGWCC 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 1 cut(s) 83
SsiI CCGC 6 cut(s) 59, 228, 419, 493, 516, 534
SspDI GGCGCC 1 cut(s) 313
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 4 cut(s) 126, 186, 278, 316
TaaI ACNGT 3 cut(s) 33, 103, 115
TaqI TCGA 3 cut(s) 269, 325, 479
TaqII GACCGA 1 cut(s) 125
TasI AATT 1 cut(s) 83
TfiI GAWTC 1 cut(s) 450
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 2 cut(s) 28, 393
TspRI CASTG 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 99
XagI CCTNNNNNAGG 1 cut(s) 419
XapI RAATTY 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 479
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.