Rroxscaffold_2G00118900

Cell wall-associated hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019640
Physical Location & Seq
Forward (+)
50792269 .. 50793584
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118900.1

Sequence Viewer

Length: 519 bp
ATGCTTTCAGCAGTTATCCGCTCCGCACTTGGCTACCCAGCGTTTACCGTGGGCACGATAACTGGTACACCAGAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTCCCCTGTCATCAGGTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTCCTGGGAGTATGGCATGGGTTACTTCAGCGCCGTGGCGCCTGGTACTCGAACATTGGCTCGGGGCATTTTCTCTACCCCTTCTTACCCTGAAAAATCAGGGGCACCTCGCGTCCTTGAACCGATAACCATCTTTCGGCTAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.3

Weight (kDa)

10.03

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019640)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07775
pyrus_communis pycom01g00110
rosa_laevigata RLG00000000161 RLG00000000418
rosa_multiflora Rmu_sc0013945.1_g000007
rosa_roxburghii Rroxscaffold_2G00118900 Rroxscaffold_2G00118910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 3 cut(s) 342, 409, 475
AccBSI CCGCTC 1 cut(s) 21
AccII CGCG 1 cut(s) 483
AciI CCGC 6 cut(s) 19, 24, 82, 155, 213, 324
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 382
AcyI GRCGYC 2 cut(s) 292, 410
AfaI GTAC 3 cut(s) 67, 344, 418
AfiI CCNNNNNNNGG 4 cut(s) 222, 284, 307, 507
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 2 cut(s) 356, 491
AjnI CCWGG 2 cut(s) 374, 412
AluBI AGCT 1 cut(s) 369
AluI AGCT 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 118, 321
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 179
Asp718I GGTACC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 404, 412
AspS9I GGNCC 1 cut(s) 195
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 99, 212, 250
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 1 cut(s) 195
AxyI CCTNAGG 1 cut(s) 191
BaeGI GKGCMC 3 cut(s) 56, 134, 478
BanI GGYRCC 3 cut(s) 342, 409, 475
BccI CCATC 1 cut(s) 509
BceAI ACGGC 3 cut(s) 200, 225, 389
BciT130I CCWGG 2 cut(s) 376, 414
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 118, 321
BfaI CTAG 1 cut(s) 517
BfoI RGCGCY 2 cut(s) 405, 413
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 224, 376, 414
Bme18I GGWCC 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 195
BmiI GGNNCC 4 cut(s) 242, 344, 411, 477
BmrFI CCNGG 3 cut(s) 224, 376, 414
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 293
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpuMI CCSGG 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 500
BsaHI GRCGYC 2 cut(s) 292, 410
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 48, 223, 375, 405
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 284, 307, 507
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 2 cut(s) 67, 288
Bse21I CCTNAGG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 2 cut(s) 376, 414
BseDI CCNNGG 4 cut(s) 48, 223, 375, 405
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 4 cut(s) 222, 284, 307, 507
BseNI ACTGG 2 cut(s) 67, 288
BseSI GKGCMC 3 cut(s) 56, 134, 478
BseX3I CGGCCG 1 cut(s) 210
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 483
Bsh1285I CGRYCG 2 cut(s) 213, 235
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 3 cut(s) 342, 409, 475
BsiEI CGRYCG 2 cut(s) 213, 235
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 3 cut(s) 223, 238, 278
BslI CCNNNNNNNGG 4 cut(s) 222, 284, 307, 507
BsmAI GTCTC 2 cut(s) 118, 321
BsnI GGCC 1 cut(s) 212
Bso31I GGTCTC 1 cut(s) 321
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 3 cut(s) 56, 134, 478
Bsp143I GATC 1 cut(s) 136
BspACI CCGC 6 cut(s) 19, 24, 82, 155, 213, 324
BspANI GGCC 1 cut(s) 212
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 4 cut(s) 242, 344, 411, 477
BspT107I GGYRCC 3 cut(s) 342, 409, 475
BspTNI GGTCTC 1 cut(s) 321
BsrBI CCGCTC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 2 cut(s) 67, 288
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 4 cut(s) 48, 223, 375, 405
BssMI GATC 1 cut(s) 136
BssNI GRCGYC 2 cut(s) 292, 410
Bst2UI CCWGG 2 cut(s) 376, 414
Bst4CI ACNGT 3 cut(s) 49, 129, 199
BstACI GRCGYC 2 cut(s) 292, 410
BstC8I GCNNGC 2 cut(s) 239, 290
BstDEI CTNAG 1 cut(s) 191
BstDSI CCRYGG 2 cut(s) 48, 405
BstEII GGTNACC 1 cut(s) 256
BstFNI CGCG 1 cut(s) 483
BstH2I RGCGCY 2 cut(s) 405, 413
BstHHI GCGC 2 cut(s) 404, 412
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 118, 321
BstMBI GATC 1 cut(s) 136
BstMCI CGRYCG 2 cut(s) 213, 235
BstMWI GCNNNNNNNGC 2 cut(s) 152, 234
BstNI CCWGG 2 cut(s) 376, 414
BstNSI RCATGY 1 cut(s) 100
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 222, 374, 412
BstSLI GKGCMC 3 cut(s) 56, 134, 478
BstUI CGCG 1 cut(s) 483
BstZI CGGCCG 1 cut(s) 210
Bsu36I CCTNAGG 1 cut(s) 191
BsuRI GGCC 1 cut(s) 212
BtgI CCRYGG 2 cut(s) 48, 405
BtsIMutI CAGTG 2 cut(s) 134, 281
Cac8I GCNNGC 2 cut(s) 239, 290
CfoI GCGC 2 cut(s) 404, 412
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 195
CseI GACGC 2 cut(s) 281, 472
Csp6I GTAC 3 cut(s) 66, 343, 417
CviAII CATG 3 cut(s) 97, 307, 388
CviJI RGCY 9 cut(s) 33, 114, 212, 228, 241, 298, 369, 431, 511
CviKI_1 RGCY 9 cut(s) 33, 114, 212, 228, 241, 298, 369, 431, 511
CviQI GTAC 3 cut(s) 66, 343, 417
DdeI CTNAG 1 cut(s) 191
DinI GGCGCC 1 cut(s) 411
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 210
EagI CGGCCG 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 195
Eco52I CGGCCG 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 382
Eco81I CCTNAGG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 432
Eco91I GGTNACC 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 374, 412
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
FaeI CATG 3 cut(s) 100, 310, 391
FaiI YATR 6 cut(s) 98, 203, 304, 308, 384, 389
FatI CATG 3 cut(s) 96, 306, 387
FauI CCCGC 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 213
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 517
GlaI GCGC 2 cut(s) 403, 411
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 41
HaeII RGCGCY 2 cut(s) 405, 413
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 223, 238, 278
HgaI GACGC 2 cut(s) 281, 472
HhaI GCGC 2 cut(s) 404, 412
Hin1I GRCGYC 2 cut(s) 292, 410
Hin1II CATG 3 cut(s) 100, 310, 391
Hin6I GCGC 2 cut(s) 402, 410
HinP1I GCGC 2 cut(s) 402, 410
HpaII CCGG 3 cut(s) 223, 238, 278
HphI GGTGA 3 cut(s) 99, 212, 250
Hpy166II GTNNAC 3 cut(s) 45, 68, 220
Hpy188I TCNGA 2 cut(s) 123, 162
Hpy8I GTNNAC 3 cut(s) 45, 68, 220
HpyAV CCTTC 3 cut(s) 284, 350, 462
HpyCH4III ACNGT 3 cut(s) 49, 129, 199
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 234
HpyF3I CTNAG 1 cut(s) 191
Hsp92I GRCGYC 2 cut(s) 292, 410
Hsp92II CATG 3 cut(s) 100, 310, 391
HspAI GCGC 2 cut(s) 402, 410
KasI GGCGCC 1 cut(s) 409
KpnI GGTACC 1 cut(s) 346
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 136
LmnI GCTCC 2 cut(s) 26, 246
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 517
MaeIII GTNAC 4 cut(s) 140, 205, 256, 392
MalI GATC 1 cut(s) 138
MbiI CCGCTC 1 cut(s) 21
MboI GATC 1 cut(s) 136
MboII GAAGA 1 cut(s) 79
MhlI GDGCHC 3 cut(s) 56, 134, 478
MluCI AATT 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 3 cut(s) 67, 125, 489
MroNI GCCGGC 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 95
MspI CCGG 3 cut(s) 223, 238, 278
MspR9I CCNGG 3 cut(s) 224, 376, 414
MvaI CCWGG 2 cut(s) 376, 414
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 152, 234
NaeI GCCGGC 1 cut(s) 239
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 1 cut(s) 224
NdeII GATC 1 cut(s) 136
NgoMIV GCCGGC 1 cut(s) 237
NlaIII CATG 3 cut(s) 100, 310, 391
NlaIV GGNNCC 4 cut(s) 242, 344, 411, 477
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 1 cut(s) 100
PciI ACATGT 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 214
PluTI GGCGCC 1 cut(s) 413
PscI ACATGT 1 cut(s) 96
Psp6I CCWGG 2 cut(s) 374, 412
PspEI GGTNACC 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 374, 412
PspN4I GGNNCC 4 cut(s) 242, 344, 411, 477
PspPI GGNCC 1 cut(s) 195
RsaI GTAC 3 cut(s) 67, 344, 418
RsaNI GTAC 3 cut(s) 66, 343, 417
RseI CAYNNNNRTG 1 cut(s) 95
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 136
Sau96I GGNCC 1 cut(s) 195
ScrFI CCNGG 3 cut(s) 224, 376, 414
SduI GDGCHC 3 cut(s) 56, 134, 478
SetI ASST 9 cut(s) 78, 192, 258, 276, 333, 361, 371, 481, 518
SfaNI GCATC 1 cut(s) 93
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 1 cut(s) 179
SsiI CCGC 6 cut(s) 19, 24, 82, 155, 213, 324
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 1 cut(s) 517
StyD4I CCNGG 3 cut(s) 222, 374, 412
TaaI ACNGT 3 cut(s) 49, 129, 199
TaqI TCGA 2 cut(s) 365, 421
TaqII GACCGA 1 cut(s) 221
TasI AATT 1 cut(s) 179
TauI GCSGC 1 cut(s) 215
TscAI CASTG 2 cut(s) 134, 288
TseFI GTSAC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 256
TspRI CASTG 2 cut(s) 134, 288
VpaK11BI GGWCC 1 cut(s) 195
XapI RAATTY 1 cut(s) 179
XceI RCATGY 1 cut(s) 100
XspI CTAG 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.