pycom01g03940

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019242
Physical Location & Seq
Forward (+)
2994513 .. 3005113
10601 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 9354 bp
ATGTTCTTTGCTAAGAGCATAAGCCTTCTCAACTGTTTTGACGTGACGTTCGGATTTAAAGAGGTCAAAGACCATTTTTTCTTTTTTTGTTCTTCATTGACAATGTCAGGTATAGTTCCTAAGTTGGATCTAGAGCTAGTGCTGGCCATTGAACTAGATCTAAATAATCTACTCATGAATAGATTATTTAGATCTAGTTCAATGGCCAGCACTAGCTCTAGATCCAACGTAGGAACTATACCTGACATTGTCAATGAAGAACAAAAACTGGATTTTCAAACAGACGAAAGTATTGATTTTTCCGACTGGAACATTCCAAAAGTTTCAACCCAAAATATTTACAAGAAAAAATGGTCTTTGACCTCTTTTAAATCCGAACGTCACGTCAAAACAGTTGAGAAGGCTTATGCTCTTAGCAAAGAACATGAGACTTGTCAATTATTTTCTCTAGAACAAATCAAAGCGCATCGCAAAAATGGTCATAACTATCTTCACATCGGTTTAGTTCAAATCGCGGTTAAACCTTTAACACGAAAAGGTCTTAATACGTCTATTCTTTTATGTCTTCGTGACGCCCGATTTACTGATTTTAACGATAGTATTCTTGGAATGATTGAATCAAGTCTTTATAACGGACCTATCCATTTTGATTGTTTTCCAGATCTCACAATAAGTCTTTCAGACCCACATGTTTTAAAAGCTCTTACTCTTAATATCAAAACCTCCGGGTACCAAGTCCTGGAAGGAACACAACCTTTGGCACTTATTTTTAGAATTTATTACAAAGTCACTGGCACAAACATGAATTTTCAAGCTTTGAACAAAAGTCCTCGCGACCACACTCTTTTAATCCAAACAACCCAAGAAAGCGCAAACATAAGAGTACCAAGAACCATTAGGTGGTCAGACATCAGTCTCCCCTCAGATTGGTGTTTAATTAATGAAAGCAAGCCGGTTTCCGTTCAAAACAGCCTCGTTAATCTTGACAACATTGAACAATATTTTGACGGCACAGTTAAAATCAATTTTGATCGTCCAGCAAGAAAATCTTGTGAATCAGTTTTGTCACACAAATCTTTTACTCCTAGCAGACAGTCTTTTTCTGGATCCACGTCAACCGATAGACTGGGTCGAGATCAAGATTTGATCAAATCTTTAGTAAACAAAAAGTTAAAAGAGCAGGAAGCTGCACAACAAGATTTAGTCAATGATTTACTCAAATTAAAGTCTGTCGACACTTCTTCACAAGTTGCACATCCTGTTTATCATCCTCCAAACCAAGAAGAACCCACTTCTCCAACTCCTTCTGATTTTGAAACAACATCGGTGAATCACCAATTACTTGTTTTAAACAAACCACATAAAATTCGATGGAGGAAGCTTAAAGCTGACATCGCCTCTGAGGAAAACCTCCCCAGAAGAAATATTTTTAGAAAAAAGTATTGTGATGAAGAAAAGCTAGCCATATATGTCGAATGGAAAGCTTTTGTAGAACAATACCAATTTGAAATATCTTTCTTTGATTTTATTGAAAAACATTATGACACAAAAAACAAAGTTACGGTTGTTACCAAAGAAAATTGGGTTAAGGAAGACAAAACTTTGGTTCCTCTAACCAACATTCCCGCAACCCCTTTCAAAATTCCAAAAGAAGATTCTGGTTTTAAACCTGTCATTGAACAGAACAATTTTACAAACCAAAGTCTTCACACCATTGGAAAACAGTTAGACAAAATTGAAACCAAAATCGACAAATTGTCTCAACCAATTTCTTCTCCAAAAGAAAAACCTTTAGTAAATTTTACTGATACTGCTTCAAGTTCCACTGCTTTAAAATCCATGACTACTTTACAAAAAATTGATCATATGCTCACTGAGCTCAAAAAAGAGAAAGTTGTTAGTGTTTTGCAAAACAATGATGTTTCTGTCGAAGAAGAAAACACATCTGATCCAGATTCTTCTCAGGACACGGAAACTAGTTCTGCTATTCAAAATATTGAAAATGTTTTTCACAACATTGATCTTCAACCAACTCTTGATTTAAAACGTATTCGTACTCATCCGGTCAACCCTACTTCTCTCACAAAAAACTGGTATCCCAGGCCAACTCCTCCAGACCTTCAATTTGAAGAACGAAATATTCAAAATCAATTTTCAGTTTCAGCCGACAAACTTTATGAGTGGAACATCGATGGCTTATCCGAACAAGAACTTTTGAACAAACTTCAACACATGTCCATGGTTGCTAATAGCTACATTTCAAATCACAATTTTACACAAACACAAATCATTGATCTTCTCGTCACTGGTTTCACTGGAATGCTTCATTCTTGGTGGGAGAAGCACTTAACTTCTCAGTCCAGAGATGAAATACGCTTTGCGGTCAAAAAAGATGAGGAAGGATTTCCCATTTTTGACGAAACGATAGGACAGGGAATACCCGACGGAGTAAATACTTTACTTTATACGATTATAGAACACTTTATAGGAACACCCAGCAACGTTACTACACGTATTCACGACCAACTGAGTAATTTAAGGTGTCCAACCTTAAGTGATTTCAGATGGTATAAAGATGTTTTCATATCCAGAGTTATGCTTCGGGAAGATAGTAATCAACCTTTTTGGAAAGAAAAATTTATCAATGGTTTACCAAATCTTTTTGCTCACAAAATTCGAAATGTTTTAGTAAATGAAGAAGGTGTCATACCTTATCATACCCTTACTTATGGTAACATAGTTAATGTTATTCAAAAGGAAGGATTGAAAATGTGTATCGACATGAAGATTTCAAAACAAGTTCATAAAGATAAATCTATTGCTAAATACGAACTTGGAACATTTTGTGAACAATATGGTTTACCTCCAATCGCTCCTTCGAGTAAAAAGAAAAAAGCCCAACAAACCAGCAAGTTTTCTAAACATCAATCCAGATTTTATAAAAACAAAAGATCTTCCAACAAACCTTTTCAGACAAATAAATTTTATGAAAAACCATCTTCAAACAAAAAACCTTTTCGAAAGTTTAAAAAGGATTTTCAAAATAAAAAGGGAAAGTGTTACAAGTGTGGTAAATTTGGTCATTATGCAAATGAATGCAAGGTTAAAAAGGTTATTAACCAACTAAAAATCTCTGAAGAAGAGAAGGTTCAACTTATCCAGGCTTTAGAAATTAGAAATACCGACTCTAGTCAATATGATAAAAATCCCGATTTTTCCGTCTCCGACTCTAGCAGTTCTAGCGACGTCTCCTCGCCAAACATTAAGTTTGGGTGCACGGACACTTGTTGTAATAAAATTTCAGTGCTTAATAAGCAAGAAGAACAAGAAGAGTTTTTAATGGAATTAATTAGTAAAGTCGATGACCCTGATTTAAAATCTTTTTATTTGAAAAAACTCAAAAATTTGATTTCTTCTCATGAACCTGGCACTAGTCAAACTTCTTCTCAAAAGATTTCTCTCAGTACCACTTTGGAACGTTTTAATAAACCCAAAAAGGAGATCACCCTCAAAGATCTGCAAAAAGAAGTTAACCAAATCAAATCGGAAATTAAGTTTTTAAAATCCGAAAATACCGAGATTCAACAAAATGGTTCTTCCTCAAATTCCGATTCCGACGCTCACTCAGCTAAATCTTCTTCTTCAAAAGAACTTGTTTTAAGTCTTTTAGACAAAATCAGAATTCGAAAATGGTATTCTAAAGTTACGATAGTAATCGAAGATTTTCATTTAGAAACCATCGCTTTAATTGATTCAGGTGCAGATTTAAATTGCATTCAAGAAGGGTTAATACCTACTAAATATTTTCACAAGTCCACGGAAACACTTAGCGCTGCAAATCAATCCCCGATGAAATTAAAATATGAAATTCCCAAAGCCCATGTTTGTCAACAAAATATTTGTTTTAAAACTCCATTTGTTTTAATCAAAAATATTTCTGATCCAGGTATTTTGGGACTCCCTTTCATTGCATTAATTTATCCTTTCAAAGTTCATCATAACTGTATTTCTACCAAGGTTTTTGGCCAAAAAATTACTTTTGAATTTTGTATGGAAACAGATCTTAAAAAGTTAAAACAACTTCAAAAAGATAATGTTTCAAAAACCCTCAACCAGATTACTGCAAAATCCCAACAAATTGCATTTTTACAAGAAGAAATTATTCACAAAAGAATTGAAGAACAGTTGACGAACAAATCTTTGTTAAATGCTATTCGTCAGTTTTCCGATAAGCTTACTAAAGAAATTTGTTCCGATCTTCCAAATGCTTTTTGGCACAGAAAACAACATATTGTTAAACTACCTTATATTAAAGATTTTATTGAGTCAAAAATCCCCACAAAAGCACGACCAATTCAAATGAATCAAGAAGTTTTAGAGTTTTGTAAAACTGAAATTAATCAACTTTTAGAAAAAGGAATTATCCGTAAAAGCAAATCTCCATGGTCTTGCCCTGCTTTTTATGTCCAGAAAAATGCAGAATTAGAACGCGGCGTTCCACGATTAGTTATTAACTACAAACCCTTGAATGATGTTTTAGAATGGATCCGATATCCAATTCCTAACAAAAGAGATTTGATAAAAAGACTTTCTCAAGCAACTGTTTTTTCCAAATTTGATATGAAATCAGGTTTTTGGCAAATCCAAATTCATGAATCTGATAAATACAAAACTGCTTTTGTAACTCCTTTCGGACATTACGAGTGGAATGTAATGCCTTTCGGCTTGAAAAATGCACCAAGTGAATTTCAAAATATCATGAATGAAATTTTTAATCAATACAGCCATTTTTCGATTGTTTATATTGATGATGTATTAATTTTCTCAAAATCAATAGATGAGCATTGGAAGCATTTGCATGCATTTGTTAGGATCATTAGGTCCAATGGGCTAGTTGTTTCGGCATCTAAGATTAAGTTATTTCAAACCAAAGTTAGATTTTTAGGTTACCATATTTACAGGTCTACCATCCAACCAATTGATCGAGTCATCCAGTTTGCTGATAAATTTCCAGATCAAATTATTGATAAAACCCAGCTTCAAAGATTCCTTGGATCTCTCAATTATGTTTCAGAATTTTATCCTCATCTTCGTCAACAATGTAAACCTTTGTTTGATCGTCTCAAGGAGAATCCTCCATCATGGTCTCAAAATCATACTTCCATTGTTAAACAGATAAAAAATCATGTCAAGACTTTGCCTTGCCTTGGCATTCCAACTCCCGACTCTCTCAAAATAGTTGAAACCGACGCATCCGATATCGGTTATGGAGGTATCCTCAAGCAGAAAACTTCTTCCGAATCTCCTGAACAAATTGTTCGTTTCCATTCAGGAATTTGGAATCCGGCTCAAAGTAACTATAGTACTATTAAAAAAGAGATTTTATCAATTGTATTATGCATTAGCAAATTCCAAGATGATTTATTAAATCAAAAATTTTTAGTTCGTGTTGATTGCAAATCTGCAAAACATGTTTTACAAAAAGATGTTCAAAACATTGCATCAAAACAAATTTTTGCACGCTGGCAAGCCATATTAAGTATTTTTTATTTTGATATTGAATACATTAAAGGCAGCCAGAATTGCATACCAGATTTTCTAACCAGAGAATTTTTGCTCCCAGCCACCCTTACCCAAACCCCAGCACCAGTAAAACAAGAGTCTATTCCCGACTCTTCATCGGCCCTCGCCATTACAAACAGATTTACTCCCTTAGGATCAACAGTAGGAAATATCCGACCAAATTACCAAACGGCCTTAGCCACCCCTTATGATCCCTACTCTTCAGTTCGTTCTCCAGCACCTTCACAACCTAAAACACCTAGCTATGCTAAAACATCTCCATATGTCCAAAATCCCAATTCAGAAAAGCTTTTTAACATCCCTGTTCACATTGACAAAAACCAACCCCCAGAGCGCATTGCCAAATTACTCCTTCCACCAAATTATCATTTCTTACCAACTGAATCCTATAAAAGCCTAAAATATTACCAGGCTATCCTTTCAGAAACAGAAAGCATTGCTATTAAGCCAATTTACAGTACCGCTCACCAAAACAAAATATTGTATCATTCACTCCATATTGGAAAATTCCTTACCGAACTCGAATGGGGAATTCCCCTTTATCACACAAAACCCCTTCATACTCAGCATGTTCTCATCCCAAACAATCATTACAATTACTATGATTATATCCAAGCATGGACCAACATTTTTCTCCACCAGACAGAAGACTTTAGTCATTCATGGTTCATAACCTTTGATAAGAATTTCAGATTGAGGTTTCCTGCATGGTTTCCAGATTGGTGGGCTTCCCATGGCCCAAGCTCTTCTATCATTCCAGATGACCTTCGTCATGTTCTCACAAACAACTTCCAGCCCAGTTTTGACAGAAGTCGTTTCGACAACAGAGTTACTATTTTCTCACTTCTCATGGCCAAGAACAAGATCCCATGGATTGTCAAATGGAACTTTGAAGTGGACACTGGTGGTATTTACAGAACCCGATGGGTAAAATGGTGGGATAAATTCAACCACCAAAAAATTATTGATCTTGTAAAAGTGGAATTTCCCCAGGCATCTTCTCAGGCATTGCCAATCAAAGTTTCAACTTCGTCCTCACAAGCTTTGCCAGTTCATCAGCAATTTCCTGAATTACCAAAAGCTCAGGAACCAGTTCAGTCCTTATCAGACATTAGTCCATCTTCCTCTCTCAAAGGAATTTCCAAGTCAGCCAAGTCCTCCAGCTCAAAGAAAGAAAAAACTACCCAACTGCTGGATATGGCTCAGCTGCTAATGGCGGAAGCAGCATTGCTGCAAAAGAAAAAAGGTTCAGACTCAGATTCAAATAATTCTGTTGCCTCGTCTCAGCTTCCCGCCACATGGGCTGATCAAGCAGATTTTCAAGATCTATTTTCATCTCTTCCGCAAAAGACAAAGCATTATTCTTCAATAGTGAAAAACAAAAAGGTCACTATTCACAATAGTGAAAAACAAAAAGGTCACTATTCATTTAACTTTTGTAAAAGAATCAAAGCATCTTCACTATTCCTTCACCCAGCTTTAGCAGACAGCGGGACTCCCGGATCAATTGGCCAAGTCTCATTCAGTATCCTCGTTCTATATAACGAGGATTCATCTTCAGAAGAAAAATATAAGTTTTTAACTTCAAAAGCTCAACATATATATTATTCAGGATGCCTGCGAGCTCCATCTCTTTGTTTACTTGTTTCATCTGCCATTAGGCAATACCTGTTTGTTATCTGCATTACAGTAAATGCTACTCTGTTTTTATATGTTTTATTTCTGCACTTACCATATATATATATATATATGTATGTATATTCTATTTTCTGGGAAAGTATACAGGTGTTACGGCAAGGGGCTAATGCCCTTGGTTTGAAAAGTTTTGTTCACTCACTCACATTTTCTATTTTTGCACCCCTCCAAGTTCTTGCAGCAGAGTTCTGTATCGACGAGGATTCGTGGCACTTTCCAGTACAGATATCTTCTTATGATGGTATAGTCATTATCTTACCTTATTGTAGACCTATGCTTTGTATCACATGTGAAATGGGTCCAGACCCTCTCACAACGCACTCTGTACTTAGTACACTTTATGGCTTCGTCGCCTTTCAGGTGTCGGCCAGCACGCCTCGATTTAGGGTCCTAGTGGATATTTCGGGTCAGGGTGTGTTACCATGCATATCTATGTTTACATTTATTCTAAAAATAATTAATACCTTAATCCATTCATTTATTTTAAATAGGAAACAGGAATTAAATAAAAAAAAGCTTCAATTTAAGTATTGTCCTCAAAAACATAAATTTAAAACAACTATTTCGATGGTCCTCCATTCCGCTAGACATCATAACGCCACCGATTGTAACCTCCCTCCAACGCATCCTCCGTCAACTTCATCAACTGTTCATTCGTATCATCATCAAGAGTATACTTACACTATTACACATATATCCATCAACATTATACTTACTAATAATTCATCCATCACCGCCTTCTTCGCATTCTCAGACACAGATTTTCAAGAACGCCACTTAGCAGTAGGACAATTATTCTGCATGGCTACAGCAATTGCATGCGCAAATCAAAATGTTCCCTCAAATCTTTCAGATCAGCGGCTAGCACGTTCTAATCCTTTAGAAGCGCATATTTATAGGAAGGTTAGGGCCTTTGCGCGACGAAGGCTTTGTCGCGCAAACATTTGTAATAAATACAATATTGATACCTCTAATTCACCTGCAGTGAATTGTCTGACTCTTTTTCGTCACCCAAGATTTTCGCGCCAAAATAAATTACTCCACATTTTCTTGATGACTTTGCGGGACGACAACTATTGTTATGTCTTTTTTTGCGCGACGACACTTTTTTTTCCGTCACATAAGCTGTTTTTTCTACTAGTGTTTATTGTCGGGGATTCAATCGAATTGTTGATTGCTTCTTTCCGAAATGTAGCATCTGGACTAGACATAGCCTCTTTGTATGTTTATGGTTCTGTATCGGCTATCAATGTCAATAAACCAGGTCCAAACTTTGTAGGCGCTCTTGCCCTCTTTAGGTTGTGTGGTTTTTGTGTGCAAATGCCATTTCATATAATCTTGCGGCCAGGCTTGAGTTCTATGTTCCTTTCTTTCCAAATGCTCCATAAAATGACTAGCAATGCCACAGCCCCGAATTCAGCCGAAGCTAAATTCGTGAGCTCCGAAAAAAATCTAAAAGAGCTTTTACATGAAAACAATCTCCAGGAACTGAAATGCAAAATACTTCATCTCAATTTACAACTAGGGAGAAATTACAATTACAACACCAACTTTATCAAAAGGACTTTAAGGTCTTTGATAAAGTGCACGATCAACTGCACAGCCAAAACTTCAAAAGTGATACCACTACGACACGAGCTAATCCGAAATGGAAGATTAAAAAGGAGTGAGCTACAAAGCTCAGTAACCAACTATTTATGGTTTGTAATAGAAACAGAAACTGAACTTGAAATTCTAATATTTTATTTACCAAATATACTTGATTGTAAAAGCTATGTCTTTCGAACAGTATTTTCGTCCCACTCTTGTGCTTGTGGTTCTACAACGTCTGCTCGACCAGGATACAACTACCAAATTCTACAACCCGCACTGGATTATAGAACTCTAGCGAATTGCTTTGTGTGTTTACTCTCTGGAAATTTTGTACGGAAGATAAGGAGGAAGAAAAGATCATCACTTGGATACTACCTGTTCAGTGTATTTGAAAGCACACAACTCTTTCTAATAGCTGTATCTTTTAATCAAAAAGTACAACTCTTTCTAACAGCTGTGTCTTTTAATAAAAACGTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

3118

Amino Acids

357.52

Weight (kDa)

9.18

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MP PF01107 108 - 267 2e-17 Viral movement protein (MP)
DUF7588 PF24496 460 - 521 3.4e-07 Domain of unknown function (DUF7588)
DUF7746 PF24925 721 - 793 5.3e-22 Domain of unknown function (DUF7746)
Caulimovir_coat_dom PF22909 841 - 941 7.4e-10 Caulimovirus coat protein, conserved domain
RVT_1 PF00078 1497 - 1652 2.9e-37 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 1715 - 1815 1.2e-22 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 1745 - 1847 8.1e-21 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019242)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g39761
prunus_persica Prupe.4G270900_v2.0.a1
pyrus_communis pycom01g03940 pycom03g01680 pycom13g24850
rosa_multiflora Rmu_sc0000235.1_g000028 Rmu_sc0000365.1_g000044 Rmu_sc0006558.1_g000026

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 2940
AarI CACCTGC 1 cut(s) 8266
AasI GACNNNNNNGTC 1 cut(s) 8848
AatII GACGTC 1 cut(s) 3249
Acc16I TGCGCA 1 cut(s) 8101
Acc36I ACCTGC 1 cut(s) 8266
Acc65I GGTACC 1 cut(s) 729
AccB1I GGYRCC 1 cut(s) 729
AccB7I CCANNNNNTGG 3 cut(s) 739, 900, 6775
AccBSI CCGCTC 1 cut(s) 6047
AccI GTMKAC 5 cut(s) 1233, 4964, 7363, 7546, 7952
AccII CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
AclI AACGTT 3 cut(s) 2502, 3478, 9345
AcoI YGGCCR 7 cut(s) 144, 204, 4024, 6435, 7093, 7644, 8621
AcuI CTGAAG 3 cut(s) 3156, 5769, 7125
AcyI GRCGYC 2 cut(s) 573, 3246
AdeI CACNNNGTG 1 cut(s) 4742
AfeI AGCGCT 1 cut(s) 3832
AfiI CCNNNNNNNGG 7 cut(s) 739, 900, 927, 5207, 6775, 6882, 8574
AflII CTTAAG 1 cut(s) 2551
AflIII ACRYGT 5 cut(s) 688, 2232, 2510, 5470, 7565
AhdI GACNNNNNGTC 1 cut(s) 1756
AhlI ACTAGT 3 cut(s) 1976, 3431, 8416
AjiI CACGTC 3 cut(s) 43, 385, 1113
AjuI GAANNNNNNNTTGG 6 cut(s) 1639, 1671, 2860, 2892, 6589, 6621
AleI CACNNNNGTG 2 cut(s) 6984, 9084
Alw21I GWGCWC 5 cut(s) 1881, 3278, 7210, 8720, 8867
Alw26I GTCTC 9 cut(s) 422, 920, 1764, 3226, 3253, 5126, 5151, 6870, 7105
Alw44I GTGCAC 2 cut(s) 3274, 8863
AlwNI CAGNNNCTG 2 cut(s) 8767, 8999
Aor51HI AGCGCT 1 cut(s) 3832
ApaLI GTGCAC 2 cut(s) 3274, 8863
ApeKI GCWGC 7 cut(s) 1187, 3833, 5574, 6790, 6806, 6814, 7457
AseI ATTAAT 6 cut(s) 939, 3347, 3974, 4398, 4817, 7737
Asp700I GAANNNNTTC 4 cut(s) 2403, 3723, 4161, 6675
Asp718I GGTACC 1 cut(s) 729
AsuC2I CCSGG 2 cut(s) 727, 7083
AsuHPI GGTGA 8 cut(s) 1325, 1339, 3496, 6041, 7046, 8002, 8247, 8279
AsuII TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
AsuNHI GCTAGC 2 cut(s) 1459, 8140
AvaII GGWCC 7 cut(s) 635, 4881, 6204, 7577, 7666, 7849, 8543
AxyI CCTNAGG 1 cut(s) 5713
BaeGI GKGCMC 2 cut(s) 3278, 8867
BalI TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
BamHI GGATCC 2 cut(s) 1106, 4545
BanI GGYRCC 1 cut(s) 729
BanII GRGCYC 3 cut(s) 1881, 7210, 8720
BarI GAAGNNNNNNTAC 4 cut(s) 2691, 2723, 3774, 3806
BauI CACGAG 1 cut(s) 8912
BbsI GAAGAC 4 cut(s) 557, 1599, 1697, 6237
Bbv12I GWGCWC 5 cut(s) 1881, 3278, 7210, 8720, 8867
BbvI GCAGC 7 cut(s) 1174, 3820, 5586, 6777, 6801, 6818, 7469
BceAI ACGGC 3 cut(s) 1024, 5769, 7391
BciVI GTATCC 5 cut(s) 2106, 5285, 7121, 9113, 9233
BclI TGATCA 3 cut(s) 1146, 1861, 6889
BcnI CCSGG 2 cut(s) 727, 7083
BcoDI GTCTC 9 cut(s) 422, 920, 1764, 3226, 3253, 5126, 5151, 6870, 7105
BcuI ACTAGT 3 cut(s) 1976, 3431, 8416
BfmI CTRYAG 3 cut(s) 5359, 8085, 8259
BfoI RGCGCY 2 cut(s) 3834, 8562
BfrI CTTAAG 1 cut(s) 2551
BfuAI ACCTGC 1 cut(s) 8266
BfuI GTATCC 5 cut(s) 2106, 5285, 7121, 9113, 9233
BglI GCCNNNNNGGC 1 cut(s) 6884
BglII AGATCT 7 cut(s) 157, 191, 661, 2951, 3514, 4060, 6907
BlpI GCTNAGC 1 cut(s) 6786
BmcAI AGTACT 1 cut(s) 5365
Bme18I GGWCC 7 cut(s) 635, 4881, 6204, 7577, 7666, 7849, 8543
BmeRI GACNNNNNGTC 1 cut(s) 1756
BmgBI CACGTC 3 cut(s) 43, 385, 1113
BmiI GGNNCC 7 cut(s) 731, 1108, 1608, 4547, 6672, 7578, 7667
BmrI ACTGGG 2 cut(s) 1136, 6375
BmsI GCATC 9 cut(s) 475, 4913, 5261, 5510, 6587, 7046, 7187, 7912, 8483
BmtI GCTAGC 2 cut(s) 1463, 8144
BmuI ACTGGG 2 cut(s) 1136, 6375
BoxI GACNNNNGTC 6 cut(s) 912, 3189, 6237, 6351, 6393, 6696
BpiI GAAGAC 4 cut(s) 557, 1599, 1697, 6237
BplI GAGNNNNNCTC 2 cut(s) 5262, 5294
BpmI CTGGAG 4 cut(s) 2097, 5781, 6727, 8744
Bpu10I CCTNAGC 2 cut(s) 5758, 6666
Bpu1102I GCTNAGC 1 cut(s) 6786
Bpu14I TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
BpuEI CTTGAG 4 cut(s) 4578, 5108, 5264, 8650
BpuMI CCSGG 2 cut(s) 727, 7083
Bsa29I ATCGAT 1 cut(s) 2190
BsaAI YACGTR 1 cut(s) 2513
BsaBI GATNNNNATC 3 cut(s) 2613, 3204, 3713
BsaHI GRCGYC 2 cut(s) 573, 3246
BsaI GGTCTC 1 cut(s) 5151
BsaWI WCCGGW 1 cut(s) 2062
BsaXI ACNNNNNCTCC 2 cut(s) 7891, 7921
Bsc4I CCNNNNNNNGG 7 cut(s) 739, 900, 927, 5207, 6775, 6882, 8574
Bse118I RCCGGY 1 cut(s) 952
Bse21I CCTNAGG 1 cut(s) 5713
Bse3DI GCAATG 7 cut(s) 3966, 5496, 5919, 6018, 6590, 6809, 8683
Bse8I GATNNNNATC 3 cut(s) 2613, 3204, 3713
BseCI ATCGAT 1 cut(s) 2190
BseJI GATNNNNATC 3 cut(s) 2613, 3204, 3713
BseLI CCNNNNNNNGG 7 cut(s) 739, 900, 927, 5207, 6775, 6882, 8574
BseMI GCAATG 7 cut(s) 3966, 5496, 5919, 6018, 6590, 6809, 8683
BseRI GAGGAG 2 cut(s) 2100, 3241
BseSI GKGCMC 2 cut(s) 3278, 8867
BseXI GCAGC 7 cut(s) 1174, 3820, 5586, 6777, 6801, 6818, 7469
BseYI CCCAGC 5 cut(s) 2495, 5034, 5620, 5641, 7057
BsgI GTGCAG 4 cut(s) 1173, 3780, 7292, 8860
Bsh1236I CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
BshNI GGYRCC 1 cut(s) 729
BshVI ATCGAT 1 cut(s) 2190
BsiHKAI GWGCWC 5 cut(s) 1881, 3278, 7210, 8720, 8867
BsiSI CCGG 5 cut(s) 726, 953, 2063, 5345, 7083
BslFI GGGAC 4 cut(s) 3969, 7090, 8357, 9061
BslI CCNNNNNNNGG 7 cut(s) 739, 900, 927, 5207, 6775, 6882, 8574
BsmAI GTCTC 9 cut(s) 422, 920, 1764, 3226, 3253, 5126, 5151, 6870, 7105
BsmBI CGTCTC 4 cut(s) 3226, 3253, 5126, 6870
BsmFI GGGAC 4 cut(s) 3969, 7090, 8357, 9061
BsmI GAATGC 5 cut(s) 2325, 3101, 3774, 5211, 8024
Bso31I GGTCTC 1 cut(s) 5151
Bsp119I TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
Bsp1286I GDGCHC 5 cut(s) 1881, 3278, 7210, 8720, 8867
Bsp1720I GCTNAGC 1 cut(s) 6786
Bsp19I CCATGG 4 cut(s) 2238, 4442, 6316, 6452
Bsp68I TCGCGA 1 cut(s) 834
BspDI ATCGAT 1 cut(s) 2190
BspFNI CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
BspHI TCATGA 4 cut(s) 174, 3418, 4651, 4758
BspLI GGNNCC 7 cut(s) 731, 1108, 1608, 4547, 6672, 7578, 7667
BspMAI CTGCAG 1 cut(s) 8263
BspMI ACCTGC 1 cut(s) 8266
BspOI GCTAGC 2 cut(s) 1463, 8144
BspQI GCTCTTC 1 cut(s) 6334
BspT104I TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
BspT107I GGYRCC 1 cut(s) 729
BspTI CTTAAG 1 cut(s) 2551
BspTNI GGTCTC 1 cut(s) 5151
BsrBI CCGCTC 1 cut(s) 6047
BsrDI GCAATG 7 cut(s) 3966, 5496, 5919, 6018, 6590, 6809, 8683
BsrFI RCCGGY 1 cut(s) 952
BssAI RCCGGY 1 cut(s) 952
BssNAI GTATAC 2 cut(s) 7364, 7953
BssNI GRCGYC 2 cut(s) 573, 3246
BssSI CACGAG 1 cut(s) 8912
BssT1I CCWWGG 8 cut(s) 2238, 4014, 4442, 5050, 5206, 6316, 6452, 7393
Bst1107I GTATAC 2 cut(s) 7364, 7953
Bst2BI CACGAG 1 cut(s) 8912
Bst6I CTCTTC 6 cut(s) 3135, 3324, 5680, 5788, 6334, 6927
BstACI GRCGYC 2 cut(s) 573, 3246
BstAFI CTTAAG 1 cut(s) 2551
BstBAI YACGTR 1 cut(s) 2513
BstBI TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
BstDSI CCRYGG 5 cut(s) 2238, 3816, 4442, 6316, 6452
BstEII GGTNACC 1 cut(s) 4946
BstFNI CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
BstH2I RGCGCY 2 cut(s) 3834, 8562
BstMAI GTCTC 9 cut(s) 422, 920, 1764, 3226, 3253, 5126, 5151, 6870, 7105
BstNSI RCATGY 7 cut(s) 692, 2236, 4862, 5474, 6155, 7569, 8100
BstPAI GACNNNNGTC 6 cut(s) 912, 3189, 6237, 6351, 6393, 6696
BstPI GGTNACC 1 cut(s) 4946
BstSFI CTRYAG 3 cut(s) 5359, 8085, 8259
BstSLI GKGCMC 2 cut(s) 3278, 8867
BstUI CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
BstV1I GCAGC 7 cut(s) 1174, 3820, 5586, 6777, 6801, 6818, 7469
BstV2I GAAGAC 4 cut(s) 557, 1599, 1697, 6237
BstXI CCANNNNNNTGG 1 cut(s) 6306
BstZ17I GTATAC 2 cut(s) 7364, 7953
Bsu15I ATCGAT 1 cut(s) 2190
Bsu36I CCTNAGG 1 cut(s) 5713
BsuI GTATCC 5 cut(s) 2106, 5285, 7121, 9113, 9233
BsuTUI ATCGAT 1 cut(s) 2190
BtgI CCRYGG 5 cut(s) 2238, 3816, 4442, 6316, 6452
BtgZI GCGATG 3 cut(s) 452, 1378, 3724
BtrI CACGTC 3 cut(s) 43, 385, 1113
BtsI GCAGTG 2 cut(s) 1824, 8268
BtuMI TCGCGA 1 cut(s) 834
BveI ACCTGC 1 cut(s) 8266
CaiI CAGNNNCTG 2 cut(s) 8767, 8999
CciI TCATGA 4 cut(s) 174, 3418, 4651, 4758
Cfr10I RCCGGY 1 cut(s) 952
ClaI ATCGAT 1 cut(s) 2190
CseI GACGC 3 cut(s) 581, 3626, 5258
CsiI ACCWGGT 1 cut(s) 8539
DraIII CACNNNGTG 1 cut(s) 4742
DrdI GACNNNNNNGTC 1 cut(s) 8848
DriI GACNNNNNGTC 1 cut(s) 1756
DseDI GACNNNNNNGTC 1 cut(s) 8848
EaeI YGGCCR 7 cut(s) 144, 204, 4024, 6435, 7093, 7644, 8621
Eam1104I CTCTTC 6 cut(s) 3135, 3324, 5680, 5788, 6334, 6927
Eam1105I GACNNNNNGTC 1 cut(s) 1756
EarI CTCTTC 6 cut(s) 3135, 3324, 5680, 5788, 6334, 6927
EciI GGCGGA 1 cut(s) 6815
Ecl136II GAGCTC 3 cut(s) 1879, 7208, 8718
Eco130I CCWWGG 8 cut(s) 2238, 4014, 4442, 5050, 5206, 6316, 6452, 7393
Eco24I GRGCYC 3 cut(s) 1881, 7210, 8720
Eco31I GGTCTC 1 cut(s) 5151
Eco32I GATATC 3 cut(s) 4553, 5260, 7506
Eco47I GGWCC 7 cut(s) 635, 4881, 6204, 7577, 7666, 7849, 8543
Eco47III AGCGCT 1 cut(s) 3832
Eco53kI GAGCTC 3 cut(s) 1879, 7208, 8718
Eco57I CTGAAG 3 cut(s) 3156, 5769, 7125
Eco81I CCTNAGG 1 cut(s) 5713
Eco91I GGTNACC 1 cut(s) 4946
EcoICRI GAGCTC 3 cut(s) 1879, 7208, 8718
EcoO109I RGGNCCY 2 cut(s) 7666, 8187
EcoO65I GGTNACC 1 cut(s) 4946
EcoRI GAATTC 3 cut(s) 3681, 6114, 8692
EcoRV GATATC 3 cut(s) 4553, 5260, 7506
EcoT14I CCWWGG 8 cut(s) 2238, 4014, 4442, 5050, 5206, 6316, 6452, 7393
EcoT22I ATGCAT 3 cut(s) 4864, 5402, 7706
EcoT38I GRGCYC 3 cut(s) 1881, 7210, 8720
ErhI CCWWGG 8 cut(s) 2238, 4014, 4442, 5050, 5206, 6316, 6452, 7393
Esp3I CGTCTC 4 cut(s) 3226, 3253, 5126, 6870
FalI AAGNNNNNCTT 2 cut(s) 1033, 1065
FaqI GGGAC 4 cut(s) 3969, 7090, 8357, 9061
FauI CCCGC 5 cut(s) 1633, 6883, 7067, 8334, 9151
FauNDI CATATG 2 cut(s) 1866, 5845
FbaI TGATCA 3 cut(s) 1146, 1861, 6889
FblI GTMKAC 5 cut(s) 1233, 4964, 7363, 7546, 7952
FriOI GRGCYC 3 cut(s) 1881, 7210, 8720
FspI TGCGCA 1 cut(s) 8101
GsaI CCCAGC 5 cut(s) 2499, 5038, 5624, 5645, 7061
GsuI CTGGAG 4 cut(s) 2097, 5781, 6727, 8744
HaeII RGCGCY 2 cut(s) 3834, 8562
HapII CCGG 5 cut(s) 726, 953, 2063, 5345, 7083
HgaI GACGC 3 cut(s) 581, 3626, 5258
Hin1I GRCGYC 2 cut(s) 573, 3246
HincII GTYRAC 8 cut(s) 1116, 1234, 2068, 3532, 3890, 4188, 5096, 7914
HindII GTYRAC 8 cut(s) 1116, 1234, 2068, 3532, 3890, 4188, 5096, 7914
HindIII AAGCTT 7 cut(s) 813, 1379, 1482, 4232, 5870, 6624, 7793
HpaI GTTAAC 1 cut(s) 3532
HpaII CCGG 5 cut(s) 726, 953, 2063, 5345, 7083
HphI GGTGA 8 cut(s) 1325, 1339, 3496, 6041, 7046, 8002, 8247, 8279
Hpy99I CGWCG 8 cut(s) 2447, 3248, 3620, 5252, 7478, 7631, 8202, 8380
Hsp92I GRCGYC 2 cut(s) 573, 3246
KpnI GGTACC 1 cut(s) 733
Ksp22I TGATCA 3 cut(s) 1146, 1861, 6889
KspAI GTTAAC 1 cut(s) 3532
LguI GCTCTTC 1 cut(s) 6334
LmnI GCTCC 5 cut(s) 2878, 5622, 7213, 8664, 8723
Lsp1109I GCAGC 7 cut(s) 1174, 3820, 5586, 6777, 6801, 6818, 7469
LweI GCATC 9 cut(s) 475, 4913, 5261, 5510, 6587, 7046, 7187, 7912, 8483
MabI ACCWGGT 1 cut(s) 8539
MbiI CCGCTC 1 cut(s) 6047
MfeI CAATTG 4 cut(s) 4977, 5388, 7089, 8091
MhlI GDGCHC 5 cut(s) 1881, 3278, 7210, 8720, 8867
MlsI TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
MluNI TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
Mox20I TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
Mph1103I ATGCAT 3 cut(s) 4864, 5402, 7706
MroXI GAANNNNTTC 4 cut(s) 2403, 3723, 4161, 6675
MscI TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
MslI CAYNNNNRTG 7 cut(s) 800, 2237, 2318, 4857, 6984, 7709, 9084
Msp20I TGGCCA 5 cut(s) 146, 206, 4026, 6437, 7095
MspA1I CMGCKG 4 cut(s) 6790, 7074, 8137, 9326
MspCI CTTAAG 1 cut(s) 2551
MspI CCGG 5 cut(s) 726, 953, 2063, 5345, 7083
MunI CAATTG 4 cut(s) 4977, 5388, 7089, 8091
Mva1269I GAATGC 5 cut(s) 2325, 3101, 3774, 5211, 8024
MvnI CGCG 7 cut(s) 515, 834, 4491, 8197, 8214, 8302, 8375
NciI CCSGG 2 cut(s) 727, 7083
NcoI CCATGG 4 cut(s) 2238, 4442, 6316, 6452
NdeI CATATG 2 cut(s) 1866, 5845
NheI GCTAGC 2 cut(s) 1459, 8140
NlaIV GGNNCC 7 cut(s) 731, 1108, 1608, 4547, 6672, 7578, 7667
NruI TCGCGA 1 cut(s) 834
NsbI TGCGCA 1 cut(s) 8101
NsiI ATGCAT 3 cut(s) 4864, 5402, 7706
NspI RCATGY 7 cut(s) 692, 2236, 4862, 5474, 6155, 7569, 8100
NspV TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
OliI CACNNNNGTG 2 cut(s) 6984, 9084
PacI TTAATTAA 1 cut(s) 939
PaeI GCATGC 2 cut(s) 4862, 8100
PagI TCATGA 4 cut(s) 174, 3418, 4651, 4758
PaqCI CACCTGC 1 cut(s) 8266
PciI ACATGT 4 cut(s) 688, 2232, 5470, 7565
PciSI GCTCTTC 1 cut(s) 6334
PcsI WCGNNNNNNNCGW 4 cut(s) 574, 4698, 7907, 8718
PctI GAATGC 5 cut(s) 2325, 3101, 3774, 5211, 8024
PdmI GAANNNNTTC 4 cut(s) 2403, 3723, 4161, 6675
PflFI GACNNNGTC 3 cut(s) 103, 248, 1128
PflMI CCANNNNNTGG 3 cut(s) 739, 900, 6775
PfoI TCCNGGA 3 cut(s) 738, 7081, 8760
Ppu21I YACGTR 1 cut(s) 2513
PpuMI RGGWCCY 1 cut(s) 7666
PscI ACATGT 4 cut(s) 688, 2232, 5470, 7565
PshAI GACNNNNGTC 6 cut(s) 912, 3189, 6237, 6351, 6393, 6696
PshBI ATTAAT 6 cut(s) 939, 3347, 3974, 4398, 4817, 7737
PsiI TTATAA 2 cut(s) 630, 2940
Psp124BI GAGCTC 3 cut(s) 1881, 7210, 8720
Psp1406I AACGTT 3 cut(s) 2502, 3478, 9345
Psp5II RGGWCCY 1 cut(s) 7666
PspEI GGTNACC 1 cut(s) 4946
PspFI CCCAGC 5 cut(s) 2495, 5034, 5620, 5641, 7057
PspN4I GGNNCC 7 cut(s) 731, 1108, 1608, 4547, 6672, 7578, 7667
PspPPI RGGWCCY 1 cut(s) 7666
PstI CTGCAG 1 cut(s) 8263
PstNI CAGNNNCTG 2 cut(s) 8767, 8999
PsyI GACNNNGTC 3 cut(s) 103, 248, 1128
PvuII CAGCTG 2 cut(s) 6790, 9326
RruI TCGCGA 1 cut(s) 834
RseI CAYNNNNRTG 7 cut(s) 800, 2237, 2318, 4857, 6984, 7709, 9084
SacI GAGCTC 3 cut(s) 1881, 7210, 8720
SalI GTCGAC 1 cut(s) 1232
SapI GCTCTTC 1 cut(s) 6334
ScaI AGTACT 1 cut(s) 5365
SduI GDGCHC 5 cut(s) 1881, 3278, 7210, 8720, 8867
SexAI ACCWGGT 1 cut(s) 8539
SfaNI GCATC 9 cut(s) 475, 4913, 5261, 5510, 6587, 7046, 7187, 7912, 8483
SfcI CTRYAG 3 cut(s) 5359, 8085, 8259
SfuI TTCGAA 4 cut(s) 2677, 3019, 3685, 9061
SinI GGWCC 7 cut(s) 635, 4881, 6204, 7577, 7666, 7849, 8543
SmiI ATTTAAAT 1 cut(s) 3768
SmiMI CAYNNNNRTG 7 cut(s) 800, 2237, 2318, 4857, 6984, 7709, 9084
SmlI CTYRAG 5 cut(s) 2551, 4593, 5123, 5279, 8629
SmoI CTYRAG 5 cut(s) 2551, 4593, 5123, 5279, 8629
SpeI ACTAGT 3 cut(s) 1976, 3431, 8416
SphI GCATGC 2 cut(s) 4862, 8100
SstI GAGCTC 3 cut(s) 1881, 7210, 8720
StyI CCWWGG 8 cut(s) 2238, 4014, 4442, 5050, 5206, 6316, 6452, 7393
SwaI ATTTAAAT 1 cut(s) 3768
TatI WGTACW 5 cut(s) 5363, 7498, 7603, 7610, 9307
TauI GCSGC 3 cut(s) 4494, 8140, 8623
TseI GCWGC 7 cut(s) 1187, 3833, 5574, 6790, 6806, 6814, 7457
Tth111I GACNNNGTC 3 cut(s) 103, 248, 1128
Van91I CCANNNNNTGG 3 cut(s) 739, 900, 6775
Vha464I CTTAAG 1 cut(s) 2551
VneI GTGCAC 2 cut(s) 3274, 8863
VpaK11BI GGWCC 7 cut(s) 635, 4881, 6204, 7577, 7666, 7849, 8543
VspI ATTAAT 6 cut(s) 939, 3347, 3974, 4398, 4817, 7737
XbaI TCTAGA 3 cut(s) 130, 218, 448
XceI RCATGY 7 cut(s) 692, 2236, 4862, 5474, 6155, 7569, 8100
XcmI CCANNNNNNNNNTGG 1 cut(s) 4269
XmiI GTMKAC 5 cut(s) 1233, 4964, 7363, 7546, 7952
XmnI GAANNNNTTC 4 cut(s) 2403, 3723, 4161, 6675
ZraI GACGTC 1 cut(s) 3247
ZrmI AGTACT 1 cut(s) 5365
Zsp2I ATGCAT 3 cut(s) 4864, 5402, 7706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.