pycom01g06230

Pentatricopeptide repeat-containing protein At4g02750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0016737
Physical Location & Seq
Reverse (-)
6339159 .. 6339998
840 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06230.4

Sequence Viewer

Length: 666 bp
ATGCCTAATGTGCTCACTGCTCAGGAAATGTTCTTCGAGTTTCTAAACCATTGTCCTAATTTATCAGGCTTTCCTTTTCTCCTTCAAATGTTTATCGAAAGCTTAACTAGTTTGTTGTGCTATGCACAAAAGCTTGATATTGGTCTGCATCCACATAAATGGACCCTTGTTTCCGTTCTTGCTGCCTGTTCTAGTCTTGCATCGCTTAAAAACGGAAGACAAGCTCTTCTGCTCATTATTAAACATGGATTTGAATCAAACCTCTCCCTATATAACGCTTTGATTACCATGTACACTTCCAGACCTCGTTCATGGAATACCATTATTGCTGCTTTTGCACAGAATGATCTCTATGAGAAAGCTCTTGATTTCTTCAACCAGATTGCGGTCTGTGGCTTAGAACCAGACGGTATAACATATCTTCGCTTGTTATCTGCTTGTGCTCATGCAGGGAAAGTGAACGAGAGCTTGAAGTTCCTTGGCTCGATGGTCTGTAGATACGGCATTGCTCCTAGATCGGAGCATTATGCTTGCTTGCTTGGCATTTTTGTTCACGTAAACGTAGAAATAGGGGAGCTGGCTGCAAAGAATATATTAGATTTGAATCCTCGTAGTTCCGGGCCTTATGTCTGGTTGTCCAACATATTCGCAACTGCCGGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

222

Amino Acids

24.52

Weight (kDa)

7.03

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016737)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20280
malus_domestica MD01G1035200.v1.1 MD15G1320700.v1.1
prunus_persica Prupe.6G192600_v2.0.a1
pyrus_communis pycom01g06230 pycom15g28170
rosa_chinensis RchiOBHm_Chr2g0112421
rosa_laevigata RLG00000017979
rosa_multiflora Rmu_sc0031542.1_g000001
rosa_rugosa Rorug02G0180400
rosa_samantha Rh2BG247100 Rh2CG238200 Rh2DG241400
rosa_wichuraiana Rw2G018100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 386
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 1 cut(s) 385
AgsI TTSAA 5 cut(s) 86, 254, 376, 472, 604
AhlI ACTAGT 1 cut(s) 107
AluBI AGCT 6 cut(s) 102, 133, 224, 362, 468, 577
AluI AGCT 6 cut(s) 102, 133, 224, 362, 468, 577
Alw21I GWGCWC 2 cut(s) 15, 445
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 3 cut(s) 182, 329, 581
AspS9I GGNCC 2 cut(s) 162, 620
AsuC2I CCSGG 1 cut(s) 619
AvaII GGWCC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 2 cut(s) 15, 445
BbvI GCAGC 3 cut(s) 169, 316, 568
BccI CCATC 1 cut(s) 481
BceAI ACGGC 1 cut(s) 517
BcnI CCSGG 1 cut(s) 619
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 108, 192, 513
BfmI CTRYAG 1 cut(s) 493
BisI GCNGC 3 cut(s) 183, 330, 582
BlsI GCNGC 3 cut(s) 184, 331, 583
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 162, 620
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 2 cut(s) 157, 209
BpiI GAAGAC 1 cut(s) 223
Bpu10I CCTNAGC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 619
BsaAI YACGTR 1 cut(s) 556
BsaBI GATNNNNATC 2 cut(s) 253, 603
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 504
Bse8I GATNNNNATC 2 cut(s) 253, 603
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 1 cut(s) 148
BseJI GATNNNNATC 2 cut(s) 253, 603
BseLI CCNNNNNNNGG 1 cut(s) 385
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 35
BseXI GCAGC 3 cut(s) 169, 316, 568
BshFI GGCC 1 cut(s) 622
BsiHKAI GWGCWC 2 cut(s) 15, 445
BsiSI CCGG 2 cut(s) 618, 657
BslI CCNNNNNNNGG 1 cut(s) 385
BsnI GGCC 1 cut(s) 622
Bsp1286I GDGCHC 2 cut(s) 15, 445
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 2 cut(s) 346, 515
BspACI CCGC 1 cut(s) 386
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 164
BspQI GCTCTTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 504
BsrGI TGTACA 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 2 cut(s) 346, 515
BssT1I CCWWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 231
BstAUI TGTACA 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 532, 536, 579
BstDEI CTNAG 2 cut(s) 21, 397
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 2 cut(s) 349, 518
BstMBI GATC 2 cut(s) 346, 515
BstMWI GCNNNNNNNGC 3 cut(s) 10, 335, 540
BstSCI CCNGG 1 cut(s) 617
BstSFI CTRYAG 1 cut(s) 493
BstV1I GCAGC 3 cut(s) 169, 316, 568
BstV2I GAAGAC 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 159
BsuRI GGCC 1 cut(s) 622
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 15
Cac8I GCNNGC 3 cut(s) 532, 536, 579
Cfr13I GGNCC 2 cut(s) 162, 620
Csp6I GTAC 1 cut(s) 292
CviAII CATG 4 cut(s) 245, 289, 312, 446
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 2 cut(s) 21, 397
DpnI GATC 2 cut(s) 348, 517
DpnII GATC 2 cut(s) 346, 515
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 4 cut(s) 248, 292, 315, 449
FatI CATG 4 cut(s) 244, 288, 311, 445
Fnu4HI GCNGC 3 cut(s) 183, 330, 582
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 3 cut(s) 183, 330, 582
FspBI CTAG 3 cut(s) 108, 192, 513
GluI GCNGC 3 cut(s) 183, 330, 582
HaeIII GGCC 1 cut(s) 622
HapII CCGG 2 cut(s) 618, 657
Hin1II CATG 4 cut(s) 248, 292, 315, 449
HindIII AAGCTT 2 cut(s) 100, 131
HinfI GANTC 2 cut(s) 254, 604
HpaII CCGG 2 cut(s) 618, 657
Hpy166II GTNNAC 4 cut(s) 294, 460, 553, 559
Hpy188I TCNGA 1 cut(s) 520
Hpy188III TCNNGA 3 cut(s) 23, 300, 365
Hpy8I GTNNAC 4 cut(s) 294, 460, 553, 559
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 2 cut(s) 555, 561
HpyCH4V TGCA 6 cut(s) 125, 148, 200, 338, 449, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 335, 540
HpyF3I CTNAG 2 cut(s) 21, 397
HpySE526I ACGT 2 cut(s) 555, 561
Hsp92II CATG 4 cut(s) 248, 292, 315, 449
Kzo9I GATC 2 cut(s) 346, 515
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 3 cut(s) 514, 520, 574
Lsp1109I GCAGC 3 cut(s) 169, 316, 568
LweI GCATC 2 cut(s) 157, 209
MaeI CTAG 3 cut(s) 108, 192, 513
MaeII ACGT 2 cut(s) 555, 561
MalI GATC 2 cut(s) 348, 517
MboI GATC 2 cut(s) 346, 515
MboII GAAGA 5 cut(s) 25, 218, 228, 364, 413
MhlI GDGCHC 2 cut(s) 15, 445
MluCI AATT 1 cut(s) 58
MmeI TCCRAC 1 cut(s) 663
MnlI CCTC 3 cut(s) 272, 315, 618
MseI TTAA 3 cut(s) 104, 207, 240
MslI CAYNNNNRTG 1 cut(s) 157
MspI CCGG 2 cut(s) 618, 657
MspR9I CCNGG 1 cut(s) 619
MwoI GCNNNNNNNGC 3 cut(s) 10, 335, 540
NciI CCSGG 1 cut(s) 619
NdeII GATC 2 cut(s) 346, 515
NlaIII CATG 4 cut(s) 248, 292, 315, 449
NlaIV GGNNCC 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 254, 604
PkrI GCNGC 3 cut(s) 184, 331, 583
Ppu21I YACGTR 1 cut(s) 556
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 2 cut(s) 162, 620
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 3 cut(s) 104, 207, 240
SatI GCNGC 3 cut(s) 183, 330, 582
Sau3AI GATC 2 cut(s) 346, 515
Sau96I GGNCC 2 cut(s) 162, 620
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 15, 445
SfaNI GCATC 2 cut(s) 157, 209
SfcI CTRYAG 1 cut(s) 493
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 1 cut(s) 58
SsiI CCGC 1 cut(s) 386
SspMI CTAG 3 cut(s) 108, 192, 513
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 2 cut(s) 558, 564
TaqI TCGA 3 cut(s) 36, 96, 485
TasI AATT 1 cut(s) 58
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 2 cut(s) 254, 604
Tru1I TTAA 3 cut(s) 104, 207, 240
Tru9I TTAA 3 cut(s) 104, 207, 240
TscAI CASTG 1 cut(s) 22
TseI GCWGC 3 cut(s) 182, 329, 581
TspDTI ATGAA 1 cut(s) 300
TspGWI ACGGA 2 cut(s) 163, 228
TspRI CASTG 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 162
XspI CTAG 3 cut(s) 108, 192, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.