pycom01g09420

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013865
Physical Location & Seq
Reverse (-)
10327468 .. 10329123
1656 bp
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UTR
Exon/CDS
Intron
pycom01g09420.1

Sequence Viewer

Length: 1656 bp
ATGGAGATTGCGACGGCGTTGCTGGTTTTGACAGCGATCACGGCTTACCTCATTTGGTTAACCTTCATCTCACGGTCGCTGAAGGGTCCACGTGTCTGGGCGTTACTGGGTAGTCTGCCGGGGCTCATCGAAAACTCGGACCGGTTGCACGACTGGATCTACGACAACCTTCGCGCCTGCGGCGGCACGTACCAAACCTGCATCTGCGCCATCCCCTTCCTCGCCAAAAAGCAAGGCCTCGTGACCGTCACGTGCGACCCGAAGAATCTGGAGCACATTCTCAAGACCCGGTTCGACAACTACCCCAAGGGCCCCACCTGGCAATCAGTGTTTCACGAGCTGTTGGGTGAGGGCATCTTCAACTCGGATGGCGACACGTGGCTGTTTCAGAGGAAGACGGCCGCACTTGAATTCACCACCAGGACGCTGCGCCAAGCCATGGCTCGGTGGGTCAGCCGAGCCATCAAGCTCAGGTTCTGCCCGATTCTCAAGTCGGCTGAGCTTCGAGCTGAGCCGGTTGATCTCCAAGACCTGTTACTTCGGCTCACTTTCGATAACATATGCGGTTTGGCTTTTGGGAAGGACCCGGAGACTTGCGCCCCGGGTTTTCCTGACAATGGCTTTGCCACAGCCTTCGACCGAGCCACCGAAGCCTCACTGCAGAGGTTTATCCTGCCAGAGGTGTTGTGGAAGGTGAAAAAATGGCTTGGGCTTGGAATGGAAGTCACATTGAGCCGAAACCTTGTGCATATTGAGGAGTATTTATCCGATGTGATTGCATCCCGTAAGCTCGAGTTGCTGAGTCAGCAAAAAGATGGGAACCCACACGATGATATGTTGTCAAGGTTCATGAAGAAAAAAGAGAACTACTCGGACACTTTTCTCCAACACGTGGCGCTCAATTTCATCCTAGCTGGACGCGACACGTCATCCGCTGCGCTGAGTTGGTTTTTTTGGTTGATCACTCTTAATCCAATAATTGAAGAAAAAATCCTGCACGAAATTTGCACTGTTCTGATCGAGACACGTGGTGATGATGTGGCAAGTTGGTTGGAGGAGCCATTGGAATTTGAGGAAATTGACCGATTGATATACCTCAAGGCAGCATTGTCCGAGAGCCTTAGGTTGTACCCTTCCGTACCCGAGGACTCGAAGCATGTGGCGTGCGACGACGTTTTGCCGGATGGCACATTTGTCCCCGCCGGATCATCGGTGACATACTCCATATACGCAACGGGGCGGATGAAATCGACGTGGGGAGACGATTGTCTAGAGTTTCGCCCGGAGAGGTGGCTATCTCCCGATGGTAAGAAGTTCATAATGCATGACTCAAATAAATTTGTGGCATTCAACGCCGGTCCAAGAATATGTCTAGGGAAGGACTTGGCTTACTTGCAAATGAAGTCAGTAACCGCATCGGTTTTGCTCCGCCACCGTCTCACAGTGGTGCCGGGCCACAAGGTGGAGCAAAAGATGTCGTTAACGTTGTTCATGAAGTATGGACTCATGGTGAATGTGCACAAAAGGGAGCTGGGGGCAATTTTATCAAGCATTAAAAAGGAGATGACGAAGAGACAATTGCAAGGGGAAGAGCAAGAGTGTGTGGCCGTTGAATGTAACGGCGATGGTGGTGGAGCGGTGGTTGGGGTGGCTTAA

Protein Analysis

552

Amino Acids

62.09

Weight (kDa)

6.35

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 30 - 495 1e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01600 AT2G45970 AT4G00360
fragaria_vesca FvH4_7g14480
malus_domestica MD01G1064900.v1.1 MD07G1135500.v1.1
prunus_persica Prupe.2G171800_v2.0.a1
pyrus_communis pycom01g09420 pycom07g13270
rosa_chinensis RchiOBHm_Chr1g0355891
rosa_laevigata RLG00000028118
rosa_roxburghii Rroxscaffold_4G00299940
rosa_rugosa Rorug01G0248200
rosa_samantha Rh1AG261500 Rh1BG229800 Rh1DG257800
rosa_wichuraiana Rw1G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 1447
AccB7I CCANNNNNTGG 3 cut(s) 439, 892, 1462
AccBSI CCGCTC 1 cut(s) 1637
AccII CGCG 2 cut(s) 174, 921
AclI AACGTT 1 cut(s) 1484
AclWI GGATC 2 cut(s) 164, 1213
AcoI YGGCCR 2 cut(s) 399, 1605
AcsI RAATTY 4 cut(s) 410, 1002, 1067, 1337
AcuI CTGAAG 1 cut(s) 101
AcvI CACGTG 5 cut(s) 92, 252, 378, 892, 1028
AdeI CACNNNGTG 2 cut(s) 1031, 1462
AfaI GTAC 3 cut(s) 191, 1130, 1140
AfiI CCNNNNNNNGG 6 cut(s) 439, 444, 617, 679, 892, 1462
AflIII ACRYGT 5 cut(s) 91, 375, 889, 924, 1025
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 5 cut(s) 361, 410, 983, 1351, 1613
AjiI CACGTC 2 cut(s) 927, 1254
AjnI CCWGG 2 cut(s) 317, 419
AleI CACNNNNGTG 1 cut(s) 1445
AluBI AGCT 7 cut(s) 340, 469, 502, 509, 790, 914, 1531
AluI AGCT 7 cut(s) 340, 469, 502, 509, 790, 914, 1531
Alw21I GWGCWC 2 cut(s) 276, 1521
Alw26I GTCTC 5 cut(s) 584, 1016, 1254, 1442, 1567
Alw44I GTGCAC 1 cut(s) 1517
AlwI GGATC 2 cut(s) 164, 1213
AlwNI CAGNNNCTG 1 cut(s) 477
Ama87I CYCGRG 3 cut(s) 601, 791, 1142
AoxI GGCC 5 cut(s) 235, 310, 399, 1453, 1605
ApaI GGGCCC 1 cut(s) 314
ApaLI GTGCAC 1 cut(s) 1517
ApeKI GCWGC 3 cut(s) 427, 935, 1103
ApoI RAATTY 4 cut(s) 410, 1002, 1067, 1337
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
AsiGI ACCGGT 1 cut(s) 141
AspLEI GCGC 6 cut(s) 176, 209, 432, 599, 898, 940
AspS9I GGNCC 7 cut(s) 86, 139, 310, 311, 583, 1358, 1453
AsuC2I CCSGG 7 cut(s) 120, 289, 587, 602, 603, 1283, 1452
AsuHPI GGTGA 6 cut(s) 359, 406, 706, 1043, 1225, 1522
AvaI CYCGRG 3 cut(s) 601, 791, 1142
AvaII GGWCC 4 cut(s) 86, 139, 583, 1358
AxyI CCTNAGG 1 cut(s) 1121
BaeGI GKGCMC 2 cut(s) 314, 1521
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 2 cut(s) 126, 314
BauI CACGAG 2 cut(s) 239, 335
BbrPI CACGTG 5 cut(s) 92, 252, 378, 892, 1028
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 276, 1521
BbvI GCAGC 3 cut(s) 414, 922, 1115
BccI CCATC 7 cut(s) 218, 362, 470, 809, 1178, 1298, 1619
BceAI ACGGC 5 cut(s) 30, 57, 414, 1592, 1636
BcgI CGANNNNNNTGC 3 cut(s) 35, 758, 792
BciT130I CCWGG 2 cut(s) 319, 421
BclI TGATCA 1 cut(s) 960
BcnI CCSGG 7 cut(s) 120, 289, 587, 602, 603, 1283, 1452
BcoDI GTCTC 5 cut(s) 584, 1016, 1254, 1442, 1567
BfaI CTAG 3 cut(s) 911, 1271, 1373
BfmI CTRYAG 1 cut(s) 659
BfoI RGCGCY 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 6 cut(s) 181, 184, 402, 428, 936, 1104
BlpI GCTNAGC 2 cut(s) 498, 510
BlsI GCNGC 6 cut(s) 182, 185, 403, 429, 937, 1105
Bme1390I CCNGG 9 cut(s) 120, 289, 319, 421, 587, 602, 603, 1283, 1452
Bme18I GGWCC 4 cut(s) 86, 139, 583, 1358
BmeT110I CYCGRG 3 cut(s) 601, 791, 1142
BmgBI CACGTC 2 cut(s) 927, 1254
BmgT120I GGNCC 7 cut(s) 86, 139, 310, 311, 583, 1358, 1453
BmiI GGNNCC 7 cut(s) 87, 312, 313, 585, 821, 1059, 1449
BmrFI CCNGG 9 cut(s) 120, 289, 319, 421, 587, 602, 603, 1283, 1452
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 4 cut(s) 210, 363, 788, 1424
BmuI ACTGGG 1 cut(s) 116
BoxI GACNNNNGTC 1 cut(s) 1266
BpiI GAAGAC 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 854, 886
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 1 cut(s) 470
Bpu1102I GCTNAGC 2 cut(s) 498, 510
BpuEI CTTGAG 3 cut(s) 266, 473, 1082
BpuMI CCSGG 7 cut(s) 120, 289, 587, 602, 603, 1283, 1452
BsaAI YACGTR 6 cut(s) 92, 189, 252, 378, 892, 1028
BsaJI CCNNGG 6 cut(s) 119, 306, 438, 600, 601, 1143
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 1626, 1656
Bsc4I CCNNNNNNNGG 6 cut(s) 439, 444, 617, 679, 892, 1462
Bse118I RCCGGY 3 cut(s) 141, 514, 1355
Bse1I ACTGG 2 cut(s) 111, 158
Bse21I CCTNAGG 1 cut(s) 1121
BseBI CCWGG 2 cut(s) 319, 421
BseDI CCNNGG 6 cut(s) 119, 306, 438, 600, 601, 1143
BseGI GGATG 7 cut(s) 210, 373, 779, 906, 929, 1189, 1248
BseLI CCNNNNNNNGG 6 cut(s) 439, 444, 617, 679, 892, 1462
BseMII CTCAG 5 cut(s) 484, 489, 501, 791, 932
BseNI ACTGG 2 cut(s) 111, 158
BseRI GAGGAG 2 cut(s) 770, 1070
BseSI GKGCMC 2 cut(s) 314, 1521
BseX3I CGGCCG 1 cut(s) 399
BseXI GCAGC 3 cut(s) 414, 922, 1115
BseYI CCCAGC 1 cut(s) 1531
BsgI GTGCAG 1 cut(s) 980
Bsh1236I CGCG 2 cut(s) 174, 921
Bsh1285I CGRYCG 3 cut(s) 77, 402, 640
BshFI GGCC 5 cut(s) 237, 312, 401, 1455, 1607
BshNI GGYRCC 1 cut(s) 1447
BshTI ACCGGT 1 cut(s) 141
BsiEI CGRYCG 3 cut(s) 77, 402, 640
BsiHKAI GWGCWC 2 cut(s) 276, 1521
BsiHKCI CYCGRG 3 cut(s) 601, 791, 1142
BslFI GGGAC 1 cut(s) 1181
BslI CCNNNNNNNGG 6 cut(s) 439, 444, 617, 679, 892, 1462
BsmAI GTCTC 5 cut(s) 584, 1016, 1254, 1442, 1567
BsmBI CGTCTC 2 cut(s) 1254, 1442
BsmFI GGGAC 1 cut(s) 1181
BsmI GAATGC 1 cut(s) 1346
BsnI GGCC 5 cut(s) 237, 312, 401, 1455, 1607
BsoBI CYCGRG 3 cut(s) 601, 791, 1142
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 4 cut(s) 126, 276, 314, 1521
Bsp143I GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
Bsp1720I GCTNAGC 2 cut(s) 498, 510
Bsp19I CCATGG 1 cut(s) 438
BspANI GGCC 5 cut(s) 237, 312, 401, 1455, 1607
BspCNI CTCAG 5 cut(s) 483, 490, 502, 792, 933
BspFNI CGCG 2 cut(s) 174, 921
BspHI TCATGA 2 cut(s) 849, 1491
BspLI GGNNCC 7 cut(s) 87, 312, 313, 585, 821, 1059, 1449
BspMAI CTGCAG 1 cut(s) 663
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 164, 1213
BspQI GCTCTTC 1 cut(s) 1584
BspT107I GGYRCC 1 cut(s) 1447
BsrBI CCGCTC 1 cut(s) 1637
BsrFI RCCGGY 3 cut(s) 141, 514, 1355
BsrI ACTGG 2 cut(s) 111, 158
BssAI RCCGGY 3 cut(s) 141, 514, 1355
BssECI CCNNGG 6 cut(s) 119, 306, 438, 600, 601, 1143
BssMI GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
BssSI CACGAG 2 cut(s) 239, 335
BssT1I CCWWGG 2 cut(s) 306, 438
Bst2BI CACGAG 2 cut(s) 239, 335
Bst2UI CCWGG 2 cut(s) 319, 421
Bst4CI ACNGT 5 cut(s) 75, 247, 1012, 1436, 1444
Bst6I CTCTTC 2 cut(s) 1565, 1584
BstBAI YACGTR 6 cut(s) 92, 189, 252, 378, 892, 1028
BstC8I GCNNGC 2 cut(s) 178, 1165
BstDEI CTNAG 6 cut(s) 470, 498, 510, 800, 941, 1121
BstDSI CCRYGG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 7 cut(s) 210, 373, 779, 906, 929, 1189, 1248
BstFNI CGCG 2 cut(s) 174, 921
BstH2I RGCGCY 1 cut(s) 899
BstHHI GCGC 6 cut(s) 176, 209, 432, 599, 898, 940
BstKTI GATC 6 cut(s) 39, 159, 523, 963, 1020, 1208
BstMAI GTCTC 5 cut(s) 584, 1016, 1254, 1442, 1567
BstMBI GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
BstMCI CGRYCG 3 cut(s) 77, 402, 640
BstMWI GCNNNNNNNGC 6 cut(s) 41, 180, 650, 796, 805, 1352
BstNI CCWGG 2 cut(s) 319, 421
BstNSI RCATGY 1 cut(s) 1160
BstPAI GACNNNNGTC 1 cut(s) 1266
BstSCI CCNGG 9 cut(s) 118, 287, 317, 419, 585, 600, 601, 1281, 1450
BstSFI CTRYAG 1 cut(s) 659
BstSLI GKGCMC 2 cut(s) 314, 1521
BstUI CGCG 2 cut(s) 174, 921
BstV1I GCAGC 3 cut(s) 414, 922, 1115
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 1 cut(s) 156
BstZI CGGCCG 1 cut(s) 399
Bsu36I CCTNAGG 1 cut(s) 1121
BsuRI GGCC 5 cut(s) 237, 312, 401, 1455, 1607
BtgI CCRYGG 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 1638
BtrI CACGTC 2 cut(s) 927, 1254
BtsCI GGATG 7 cut(s) 210, 373, 779, 906, 929, 1189, 1248
BtsI GCAGTG 1 cut(s) 656
BtsIMutI CAGTG 4 cut(s) 333, 656, 1008, 1449
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 178, 1165
CaiI CAGNNNCTG 1 cut(s) 477
CciI TCATGA 2 cut(s) 849, 1491
CfoI GCGC 6 cut(s) 176, 209, 432, 599, 898, 940
Cfr10I RCCGGY 3 cut(s) 141, 514, 1355
Cfr13I GGNCC 7 cut(s) 86, 139, 310, 311, 583, 1358, 1453
Cfr9I CCCGGG 1 cut(s) 601
CpoI CGGWCCG 1 cut(s) 139
CseI GACGC 2 cut(s) 433, 927
Csp6I GTAC 3 cut(s) 190, 1129, 1139
CspAI ACCGGT 1 cut(s) 141
CspI CGGWCCG 1 cut(s) 139
CviAII CATG 6 cut(s) 439, 850, 1157, 1325, 1492, 1507
CviQI GTAC 3 cut(s) 190, 1129, 1139
DdeI CTNAG 6 cut(s) 470, 498, 510, 800, 941, 1121
DpnI GATC 6 cut(s) 38, 158, 522, 962, 1019, 1207
DpnII GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
DraIII CACNNNGTG 2 cut(s) 1031, 1462
EaeI YGGCCR 2 cut(s) 399, 1605
EagI CGGCCG 1 cut(s) 399
Eam1104I CTCTTC 2 cut(s) 1565, 1584
EarI CTCTTC 2 cut(s) 1565, 1584
EciI GGCGGA 2 cut(s) 1255, 1418
EclXI CGGCCG 1 cut(s) 399
Eco130I CCWWGG 2 cut(s) 306, 438
Eco147I AGGCCT 1 cut(s) 237
Eco24I GRGCYC 2 cut(s) 126, 314
Eco47I GGWCC 4 cut(s) 86, 139, 583, 1358
Eco52I CGGCCG 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 101
Eco72I CACGTG 5 cut(s) 92, 252, 378, 892, 1028
Eco81I CCTNAGG 1 cut(s) 1121
Eco88I CYCGRG 3 cut(s) 601, 791, 1142
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoO109I RGGNCCY 3 cut(s) 310, 311, 583
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 317, 419
EcoT14I CCWWGG 2 cut(s) 306, 438
EcoT22I ATGCAT 1 cut(s) 1326
EcoT38I GRGCYC 2 cut(s) 126, 314
ErhI CCWWGG 2 cut(s) 306, 438
Esp3I CGTCTC 2 cut(s) 1254, 1442
FaeI CATG 6 cut(s) 442, 853, 1160, 1328, 1495, 1510
FaqI GGGAC 1 cut(s) 1181
FatI CATG 6 cut(s) 438, 849, 1156, 1324, 1491, 1506
FauI CCCGC 1 cut(s) 1207
FauNDI CATATG 1 cut(s) 560
FbaI TGATCA 1 cut(s) 960
Fnu4HI GCNGC 6 cut(s) 181, 184, 402, 428, 936, 1104
FokI GGATG 7 cut(s) 197, 380, 766, 893, 916, 1196, 1255
FriOI GRGCYC 2 cut(s) 126, 314
Fsp4HI GCNGC 6 cut(s) 181, 184, 402, 428, 936, 1104
FspBI CTAG 3 cut(s) 911, 1271, 1373
GlaI GCGC 6 cut(s) 175, 208, 431, 598, 897, 939
GluI GCNGC 6 cut(s) 181, 184, 402, 428, 936, 1104
GsaI CCCAGC 1 cut(s) 1535
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 899
HaeIII GGCC 5 cut(s) 237, 312, 401, 1455, 1607
HgaI GACGC 2 cut(s) 433, 927
HhaI GCGC 6 cut(s) 176, 209, 432, 599, 898, 940
Hin1II CATG 6 cut(s) 442, 853, 1160, 1328, 1495, 1510
Hin6I GCGC 6 cut(s) 174, 207, 430, 597, 896, 938
HinP1I GCGC 6 cut(s) 174, 207, 430, 597, 896, 938
HincII GTYRAC 2 cut(s) 60, 1482
HindII GTYRAC 2 cut(s) 60, 1482
HinfI GANTC 6 cut(s) 265, 484, 802, 1148, 1328, 1503
HpaI GTTAAC 2 cut(s) 60, 1482
HphI GGTGA 6 cut(s) 359, 406, 706, 1043, 1225, 1522
Hpy166II GTNNAC 4 cut(s) 60, 89, 1482, 1519
Hpy188I TCNGA 7 cut(s) 139, 367, 390, 769, 874, 1017, 1114
Hpy8I GTNNAC 4 cut(s) 60, 89, 1482, 1519
Hpy99I CGWCG 4 cut(s) 16, 1172, 1175, 1255
HpyAV CCTTC 9 cut(s) 73, 76, 179, 226, 574, 643, 685, 1143, 1372
HpyCH4III ACNGT 5 cut(s) 75, 247, 1012, 1436, 1444
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 180, 650, 796, 805, 1352
HpyF3I CTNAG 6 cut(s) 470, 498, 510, 800, 941, 1121
Hsp92II CATG 6 cut(s) 442, 853, 1160, 1328, 1495, 1510
HspAI GCGC 6 cut(s) 174, 207, 430, 597, 896, 938
Ksp22I TGATCA 1 cut(s) 960
KspAI GTTAAC 2 cut(s) 60, 1482
Kzo9I GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
LguI GCTCTTC 1 cut(s) 1584
LmnI GCTCC 6 cut(s) 271, 1057, 1431, 1465, 1528, 1634
Lsp1109I GCAGC 3 cut(s) 414, 922, 1115
LweI GCATC 4 cut(s) 210, 363, 788, 1424
MaeI CTAG 3 cut(s) 911, 1271, 1373
MaeIII GTNAC 8 cut(s) 102, 241, 247, 534, 724, 1213, 1408, 1616
MalI GATC 6 cut(s) 38, 158, 522, 962, 1019, 1207
MbiI CCGCTC 1 cut(s) 1637
MboI GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
MboII GAAGA 7 cut(s) 274, 349, 406, 865, 995, 1582, 1601
MfeI CAATTG 1 cut(s) 1577
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 4 cut(s) 126, 276, 314, 1521
MluCI AATT 9 cut(s) 410, 901, 978, 1002, 1067, 1077, 1337, 1539, 1577
MlyI GAGTC 4 cut(s) 811, 1142, 1322, 1497
MmeI TCCRAC 2 cut(s) 910, 1032
Mph1103I ATGCAT 1 cut(s) 1326
MseI TTAA 5 cut(s) 59, 969, 1481, 1554, 1654
MslI CAYNNNNRTG 1 cut(s) 1445
MspA1I CMGCKG 1 cut(s) 935
MspR9I CCNGG 9 cut(s) 120, 289, 319, 421, 587, 602, 603, 1283, 1452
MunI CAATTG 1 cut(s) 1577
Mva1269I GAATGC 1 cut(s) 1346
MvaI CCWGG 2 cut(s) 319, 421
MvnI CGCG 2 cut(s) 174, 921
MwoI GCNNNNNNNGC 6 cut(s) 41, 180, 650, 796, 805, 1352
NciI CCSGG 7 cut(s) 120, 289, 587, 602, 603, 1283, 1452
NcoI CCATGG 1 cut(s) 438
NdeI CATATG 1 cut(s) 560
NdeII GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
NlaIII CATG 6 cut(s) 442, 853, 1160, 1328, 1495, 1510
NlaIV GGNNCC 7 cut(s) 87, 312, 313, 585, 821, 1059, 1449
NmeAIII GCCGAG 1 cut(s) 482
NmuCI GTSAC 4 cut(s) 241, 247, 724, 1213
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 1445
PaeR7I CTCGAG 1 cut(s) 791
PagI TCATGA 2 cut(s) 849, 1491
PceI AGGCCT 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 1584
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 1 cut(s) 1346
PfeI GAWTC 2 cut(s) 265, 484
PflMI CCANNNNNTGG 3 cut(s) 439, 892, 1462
PinAI ACCGGT 1 cut(s) 141
PkrI GCNGC 6 cut(s) 182, 185, 403, 429, 937, 1105
PleI GAGTC 4 cut(s) 810, 1142, 1322, 1497
PmaCI CACGTG 5 cut(s) 92, 252, 378, 892, 1028
PmlI CACGTG 5 cut(s) 92, 252, 378, 892, 1028
PpsI GAGTC 4 cut(s) 810, 1142, 1322, 1497
Ppu21I YACGTR 6 cut(s) 92, 189, 252, 378, 892, 1028
PpuMI RGGWCCY 1 cut(s) 583
PshAI GACNNNNGTC 1 cut(s) 1266
Psp1406I AACGTT 1 cut(s) 1484
Psp5II RGGWCCY 1 cut(s) 583
Psp6I CCWGG 2 cut(s) 317, 419
PspCI CACGTG 5 cut(s) 92, 252, 378, 892, 1028
PspFI CCCAGC 1 cut(s) 1531
PspGI CCWGG 2 cut(s) 317, 419
PspN4I GGNNCC 7 cut(s) 87, 312, 313, 585, 821, 1059, 1449
PspOMI GGGCCC 1 cut(s) 310
PspPI GGNCC 7 cut(s) 86, 139, 310, 311, 583, 1358, 1453
PspPPI RGGWCCY 1 cut(s) 583
PspXI VCTCGAGB 1 cut(s) 791
PstI CTGCAG 1 cut(s) 663
PstNI CAGNNNCTG 1 cut(s) 477
PsuI RGATCY 1 cut(s) 156
RsaI GTAC 3 cut(s) 191, 1130, 1140
RsaNI GTAC 3 cut(s) 190, 1129, 1139
RseI CAYNNNNRTG 1 cut(s) 1445
Rsr2I CGGWCCG 1 cut(s) 139
RsrII CGGWCCG 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 1584
SaqAI TTAA 5 cut(s) 59, 969, 1481, 1554, 1654
SatI GCNGC 6 cut(s) 181, 184, 402, 428, 936, 1104
Sau3AI GATC 6 cut(s) 36, 156, 520, 960, 1017, 1205
Sau96I GGNCC 7 cut(s) 86, 139, 310, 311, 583, 1358, 1453
SchI GAGTC 4 cut(s) 811, 1142, 1322, 1497
ScrFI CCNGG 9 cut(s) 120, 289, 319, 421, 587, 602, 603, 1283, 1452
SduI GDGCHC 4 cut(s) 126, 276, 314, 1521
SfaNI GCATC 4 cut(s) 210, 363, 788, 1424
SfcI CTRYAG 1 cut(s) 659
Sfr274I CTCGAG 1 cut(s) 791
SinI GGWCC 4 cut(s) 86, 139, 583, 1358
SlaI CTCGAG 1 cut(s) 791
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 4 cut(s) 281, 488, 791, 1097
SmoI CTYRAG 4 cut(s) 281, 488, 791, 1097
Sse9I AATT 9 cut(s) 410, 901, 978, 1002, 1067, 1077, 1337, 1539, 1577
SseBI AGGCCT 1 cut(s) 237
SspMI CTAG 3 cut(s) 911, 1271, 1373
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 9 cut(s) 118, 287, 317, 419, 585, 600, 601, 1281, 1450
StyI CCWWGG 2 cut(s) 306, 438
TaaI ACNGT 5 cut(s) 75, 247, 1012, 1436, 1444
TaqI TCGA 9 cut(s) 129, 294, 505, 552, 636, 792, 1020, 1151, 1250
TaqII GACCGA 2 cut(s) 654, 1098
TasI AATT 9 cut(s) 410, 901, 978, 1002, 1067, 1077, 1337, 1539, 1577
TauI GCSGC 3 cut(s) 183, 186, 404
TfiI GAWTC 2 cut(s) 265, 484
Tru1I TTAA 5 cut(s) 59, 969, 1481, 1554, 1654
Tru9I TTAA 5 cut(s) 59, 969, 1481, 1554, 1654
TscAI CASTG 4 cut(s) 333, 663, 1015, 1449
TseFI GTSAC 4 cut(s) 241, 247, 724, 1213
TseI GCWGC 3 cut(s) 427, 935, 1103
Tsp45I GTSAC 4 cut(s) 241, 247, 724, 1213
TspDTI ATGAA 9 cut(s) 55, 838, 866, 895, 1259, 1306, 1415, 1480, 1508
TspGWI ACGGA 1 cut(s) 1126
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 4 cut(s) 333, 663, 1015, 1449
Van91I CCANNNNNTGG 3 cut(s) 439, 892, 1462
VneI GTGCAC 1 cut(s) 1517
VpaK11BI GGWCC 4 cut(s) 86, 139, 583, 1358
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 4 cut(s) 410, 1002, 1067, 1337
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 684
XhoI CTCGAG 1 cut(s) 791
XmaI CCCGGG 1 cut(s) 601
XspI CTAG 3 cut(s) 911, 1271, 1373
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.