Rroxscaffold_4G00299940

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013865
Physical Location & Seq
Forward (+)
19855664 .. 19867012
11349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299940.1

Sequence Viewer

Length: 2019 bp
ATGAATGCAAGTAATAAGAGCAAGGTTGTGGAAAAGGGAGTTCAAGATCGGGTGAGGTTGGGAACTGCAATTCGAATGGCCGTGGGGCGAAGGAGAGTTCGAAGAGCAGCCGACTTGGTCCATTCGCTCCGGTGGCAGTTCCAAGAACTAAGAAAGGTATTGACATCAACAAGGTTGTTGGGAGAGTCTTGGACTCTTGGGGCTTATTATTGGCCTCGGTATCGAACTGCCAAGGGAAAGAGAAGAGTTCCGGAACTTCATGCCACAAGCCATTACCGTGCCTGGTCCCGAAATTATCTTGGCGTGTGGGACACAGCATTGGAAATTCATTTGCAAGAGATGGGGAAGCCTTATTTAACTATGGATGTTGGCTCTGCTTTTCTCTTATTCACGGCCATCACGGCTTATCTACTTTGGTTCACCTTCATCTCACGGTCCCTGAAGGGTCCACGTGTCTGGCCCTTACTGGGTAGCCTCCCCGGCCTGATCGAAAATTGCGACCGTTTGCATGACTGGATCTGCGATAACCTACGCGCCTGCGGCGGCACGTACCAAACCTGCATCTGCGCGGTTCCATTTCTCGCCCAAAAGCAAGGCCTCGTGACCGTCACGTGCGACCCGAAGAATGTGGAGCACATCCTGAAGGCCCGGTTCGACAACTACCCGAAGGGCCCCACCTGGCAATCGGTGTTTCACGACCTTCTCGGCGAGGGGATATTTAACTCCGACGGCGAGACGTGGCGGTTCCAGAGGAAAACTGCCGCAACGGAGTTTACTAACAGGACGTTACGCCAAGCCATGGCTCGGTGGGTGAGCCGAGCCATTAAGCTGAGGTTCTGCCCGATTCTCAAGGAAGCTGAGCGAAAAGGCGAGCCGGTTGATCTTCAGGACTTGCTGCTTCGGCTCACTTTTGATAATATTTGTGGCTTGGCTTTCGGGAAAGACCCGGAGACTTGCTCTCCGGGGTATCCCGACAACAGCTTCTCCTCAGCTTTCGACCGGGCCACCGAGGCATCGCTGCAGAGGTTCATTTTGCCTGAGGTGTTGTGGAAGGTGAAGAAATGGCTCGGGCTTGGAATGGAAGTCACGCTAAGCCGGAGCCTTGTGCATATTGATGAGTATTTATCCAAAGTGATCGCTTCACGTAAGCTCGAGTTGCTGAGTCAGCAGAAGGATGGGGACCCACATGATGATCTGCTGTCAAGGTTCATGAAGAAAAAGGAGTCCTACTCGGACACCTTCCTCCAACACGTGGCACTCAATTTTATCCTAGCTGGACGTGACACGTCATCGGTGGCGCTAAGCTGGTTCTTTTGGTTAGTGACACTAAACCCGATTGTTGAGGAGAAAATCTTGCGAGAAATCTGCACCGTTCTGATTGAGACACGTGGCGAGGATGTTGCAAGTTGGTTAGAAGAGTCGTTGGACTTTGTGGAACTAGACCGTTTGATATACCTCAAGGCAGCATTGTCCGAAAGTCTTAGGTTGTACCCTTCCGTACCCCAAGACTCGAAGCATGTTGTGCGGGATGATGTTTTGCCCGACGGGACATTTGTGCCCGCCGGATCATCGGTCACGTACTCAATATATGCGACGGGGAGGATGAAGTCCTCGTGGGGCGATGATTGCCTAGAGTTTCGTCCCGAGAGGTGGTTGTCTTCGGATGGTAAGAGATTCATAATGCATGACTCCAGCAAATTCGTAGCATTCAATGCTGGTCCGAGAATCTGTCTAGGGAAGGACCTGGCGTACCTGCAAATGAAGTCGGTAGCCGCATCGGTATTGCTCCGCCACCGTCTCACGGTGGTGCCGGGCCACAAGGTGGAGCAAAAGATGTCGCTGACATTGTTCATGAAGTATGGTTTGATGATGAATGTGCATCAAAGGGATTTAGAGGGTGTTTTGGGAAGGGCAAAGAGAGACAGGGAAGTACAGCTGAATGGACAGGACTCTGTTGCTGTCAAATGTAACGGTGTTGGTGAGAACGGTGGCCGTGAAGTGGTAGTTGGGGCTGATTAA

Protein Analysis

672

Amino Acids

76.45

Weight (kDa)

9.16

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 148 - 615 2.4e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01600 AT2G45970 AT4G00360
fragaria_vesca FvH4_7g14480
malus_domestica MD01G1064900.v1.1 MD07G1135500.v1.1
prunus_persica Prupe.2G171800_v2.0.a1
pyrus_communis pycom01g09420 pycom07g13270
rosa_chinensis RchiOBHm_Chr1g0355891
rosa_laevigata RLG00000028118
rosa_roxburghii Rroxscaffold_4G00299940
rosa_rugosa Rorug01G0248200
rosa_samantha Rh1AG261500 Rh1BG229800 Rh1DG257800
rosa_wichuraiana Rw1G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 566, 1761
AccB1I GGYRCC 1 cut(s) 1807
AccB7I CCANNNNNTGG 3 cut(s) 799, 1252, 1822
AccII CGCG 2 cut(s) 534, 569
AccIII TCCGGA 1 cut(s) 250
AciI CCGC 9 cut(s) 540, 543, 569, 742, 762, 1525, 1560, 1773, 1789
AclWI GGATC 2 cut(s) 524, 1573
AcoI YGGCCR 3 cut(s) 78, 393, 1990
AcsI RAATTY 2 cut(s) 324, 1697
AcuI CTGAAG 3 cut(s) 461, 662, 869
AcvI CACGTG 4 cut(s) 452, 612, 1252, 1388
AdeI CACNNNGTG 1 cut(s) 1822
AfaI GTAC 6 cut(s) 551, 1490, 1500, 1580, 1751, 1932
AfiI CCNNNNNNNGG 8 cut(s) 467, 799, 804, 1252, 1650, 1801, 1822, 1999
AflIII ACRYGT 4 cut(s) 451, 1249, 1284, 1385
AgsI TTSAA 2 cut(s) 44, 1711
AjiI CACGTC 3 cut(s) 738, 1280, 1287
AjnI CCWGG 3 cut(s) 281, 677, 1743
AjuI GAANNNNNNNTTGG 1 cut(s) 1989
AleI CACNNNNGTG 1 cut(s) 1805
AluBI AGCT 8 cut(s) 829, 857, 981, 992, 1150, 1274, 1305, 1936
AluI AGCT 8 cut(s) 829, 857, 981, 992, 1150, 1274, 1305, 1936
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 5 cut(s) 728, 944, 1376, 1802, 1914
AlwI GGATC 2 cut(s) 524, 1573
Ama87I CYCGRG 3 cut(s) 1067, 1151, 1643
Aor13HI TCCGGA 1 cut(s) 250
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 4 cut(s) 107, 895, 1018, 1463
ApoI RAATTY 2 cut(s) 324, 1697
ArsI GACNNNNNNTTYG 2 cut(s) 1535, 1567
AspLEI GCGC 3 cut(s) 536, 569, 1300
AsuC2I CCSGG 6 cut(s) 480, 649, 947, 963, 1001, 1812
AsuHPI GGTGA 5 cut(s) 64, 412, 823, 1066, 1991
AsuII TTCGAA 2 cut(s) 73, 100
AvaI CYCGRG 3 cut(s) 1067, 1151, 1643
AvaII GGWCC 7 cut(s) 118, 285, 435, 446, 1180, 1718, 1741
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 2 cut(s) 674, 1560
BanI GGYRCC 1 cut(s) 1807
BanII GRGCYC 1 cut(s) 674
BauI CACGAG 2 cut(s) 599, 1612
BbrPI CACGTG 4 cut(s) 452, 612, 1252, 1388
BbsI GAAGAC 1 cut(s) 1650
Bbv12I GWGCWC 1 cut(s) 636
BbvCI CCTCAGC 2 cut(s) 830, 988
BbvI GCAGC 4 cut(s) 119, 882, 1005, 1475
BccI CCATC 4 cut(s) 334, 404, 1169, 1658
BceAI ACGGC 5 cut(s) 65, 408, 417, 745, 1977
BcgI CGANNNNNNTGC 4 cut(s) 1347, 1381, 1382, 1416
BciT130I CCWGG 3 cut(s) 283, 679, 1745
BciVI GTATCC 1 cut(s) 978
BcnI CCSGG 6 cut(s) 480, 649, 947, 963, 1001, 1812
BcoDI GTCTC 5 cut(s) 728, 944, 1376, 1802, 1914
BfaI CTAG 4 cut(s) 1271, 1439, 1631, 1733
BfmI CTRYAG 1 cut(s) 1019
BfoI RGCGCY 1 cut(s) 1301
BfuAI ACCTGC 2 cut(s) 566, 1761
BfuI GTATCC 1 cut(s) 978
BglI GCCNNNNNGGC 3 cut(s) 401, 480, 1010
BisI GCNGC 8 cut(s) 108, 541, 544, 762, 896, 1019, 1464, 1773
BlpI GCTNAGC 3 cut(s) 858, 1091, 1301
BlsI GCNGC 8 cut(s) 109, 542, 545, 763, 897, 1020, 1465, 1774
Bme1390I CCNGG 9 cut(s) 283, 480, 649, 679, 947, 963, 1001, 1745, 1812
Bme18I GGWCC 7 cut(s) 118, 285, 435, 446, 1180, 1718, 1741
BmeT110I CYCGRG 3 cut(s) 1067, 1151, 1643
BmgBI CACGTC 3 cut(s) 738, 1280, 1287
BmrFI CCNGG 9 cut(s) 283, 480, 649, 679, 947, 963, 1001, 1745, 1812
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 4 cut(s) 570, 1022, 1784, 1888
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 1650
BplI GAGNNNNNCTC 4 cut(s) 941, 973, 1214, 1246
BpmI CTGGAG 1 cut(s) 1675
Bpu10I CCTNAGC 2 cut(s) 830, 988
Bpu1102I GCTNAGC 3 cut(s) 858, 1091, 1301
Bpu14I TTCGAA 2 cut(s) 73, 100
BpuEI CTTGAG 2 cut(s) 833, 1442
BpuMI CCSGG 6 cut(s) 480, 649, 947, 963, 1001, 1812
BsaAI YACGTR 7 cut(s) 452, 549, 612, 1145, 1252, 1388, 1578
BsaJI CCNNGG 7 cut(s) 81, 215, 231, 478, 798, 962, 1008
BsaWI WCCGGW 2 cut(s) 129, 250
BsaXI ACNNNNNCTCC 4 cut(s) 943, 968, 973, 998
Bsc4I CCNNNNNNNGG 8 cut(s) 467, 799, 804, 1252, 1650, 1801, 1822, 1999
Bse118I RCCGGY 1 cut(s) 874
Bse1I ACTGG 2 cut(s) 471, 518
Bse21I CCTNAGG 1 cut(s) 1038
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 3 cut(s) 283, 679, 1745
BseDI CCNNGG 7 cut(s) 81, 215, 231, 478, 798, 962, 1008
BseGI GGATG 7 cut(s) 370, 636, 1180, 1402, 1534, 1608, 1669
BseLI CCNNNNNNNGG 8 cut(s) 467, 799, 804, 1252, 1650, 1801, 1822, 1999
BseMII CTCAG 5 cut(s) 821, 849, 1002, 1029, 1151
BseNI ACTGG 2 cut(s) 471, 518
BseRI GAGGAG 2 cut(s) 976, 1358
BseSI GKGCMC 2 cut(s) 674, 1560
BseXI GCAGC 4 cut(s) 119, 882, 1005, 1475
BsgI GTGCAG 1 cut(s) 1351
Bsh1236I CGCG 2 cut(s) 534, 569
Bsh1285I CGRYCG 2 cut(s) 502, 1000
BshNI GGYRCC 1 cut(s) 1807
BsiEI CGRYCG 2 cut(s) 502, 1000
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 3 cut(s) 1067, 1151, 1643
BslFI GGGAC 6 cut(s) 271, 323, 421, 1193, 1561, 1626
BslI CCNNNNNNNGG 8 cut(s) 467, 799, 804, 1252, 1650, 1801, 1822, 1999
BsmAI GTCTC 5 cut(s) 728, 944, 1376, 1802, 1914
BsmBI CGTCTC 2 cut(s) 728, 1802
BsmFI GGGAC 6 cut(s) 271, 323, 421, 1193, 1561, 1626
BsmI GAATGC 2 cut(s) 10, 1706
BsoBI CYCGRG 3 cut(s) 1067, 1151, 1643
Bsp119I TTCGAA 2 cut(s) 73, 100
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 636, 674, 1560
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
Bsp1720I GCTNAGC 3 cut(s) 858, 1091, 1301
Bsp19I CCATGG 1 cut(s) 798
BspACI CCGC 9 cut(s) 540, 543, 569, 742, 762, 1525, 1560, 1773, 1789
BspCNI CTCAG 5 cut(s) 822, 850, 1001, 1030, 1152
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 2 cut(s) 534, 569
BspHI TCATGA 2 cut(s) 1209, 1851
BspMAI CTGCAG 1 cut(s) 1023
BspMI ACCTGC 2 cut(s) 566, 1761
BspPI GGATC 2 cut(s) 524, 1573
BspQI GCTCTTC 1 cut(s) 97
BspT104I TTCGAA 2 cut(s) 73, 100
BspT107I GGYRCC 1 cut(s) 1807
BsrFI RCCGGY 1 cut(s) 874
BsrI ACTGG 2 cut(s) 471, 518
BssAI RCCGGY 1 cut(s) 874
BssECI CCNNGG 7 cut(s) 81, 215, 231, 478, 798, 962, 1008
BssMI GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
BssSI CACGAG 2 cut(s) 599, 1612
BssT1I CCWWGG 2 cut(s) 231, 798
Bst2BI CACGAG 2 cut(s) 599, 1612
Bst2UI CCWGG 3 cut(s) 283, 679, 1745
Bst6I CTCTTC 3 cut(s) 97, 238, 1410
BstAPI GCANNNNNTGC 2 cut(s) 1522, 1712
BstBAI YACGTR 7 cut(s) 452, 549, 612, 1145, 1252, 1388, 1578
BstBI TTCGAA 2 cut(s) 73, 100
BstC8I GCNNGC 3 cut(s) 538, 872, 1560
BstDEI CTNAG 9 cut(s) 149, 830, 858, 988, 1038, 1091, 1160, 1301, 1481
BstDSI CCRYGG 2 cut(s) 81, 798
BstF5I GGATG 7 cut(s) 370, 636, 1180, 1402, 1534, 1608, 1669
BstFNI CGCG 2 cut(s) 534, 569
BstH2I RGCGCY 1 cut(s) 1301
BstHHI GCGC 3 cut(s) 536, 569, 1300
BstKTI GATC 7 cut(s) 49, 489, 519, 883, 1137, 1195, 1568
BstMAI GTCTC 5 cut(s) 728, 944, 1376, 1802, 1914
BstMBI GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
BstMCI CGRYCG 2 cut(s) 502, 1000
BstNI CCWGG 3 cut(s) 283, 679, 1745
BstNSI RCATGY 1 cut(s) 1520
BstSCI CCNGG 9 cut(s) 281, 478, 647, 677, 945, 961, 999, 1743, 1810
BstSFI CTRYAG 1 cut(s) 1019
BstSLI GKGCMC 2 cut(s) 674, 1560
BstUI CGCG 2 cut(s) 534, 569
BstV1I GCAGC 4 cut(s) 119, 882, 1005, 1475
BstV2I GAAGAC 1 cut(s) 1650
BstX2I RGATCY 1 cut(s) 516
BstXI CCANNNNNNTGG 1 cut(s) 456
BstYI RGATCY 1 cut(s) 516
Bsu36I CCTNAGG 1 cut(s) 1038
BsuI GTATCC 1 cut(s) 978
BtgI CCRYGG 2 cut(s) 81, 798
BtgZI GCGATG 2 cut(s) 999, 1635
BtrI CACGTC 3 cut(s) 738, 1280, 1287
BtsCI GGATG 7 cut(s) 370, 636, 1180, 1402, 1534, 1608, 1669
BveI ACCTGC 2 cut(s) 566, 1761
Cac8I GCNNGC 3 cut(s) 538, 872, 1560
CciI TCATGA 2 cut(s) 1209, 1851
CfoI GCGC 3 cut(s) 536, 569, 1300
Cfr10I RCCGGY 1 cut(s) 874
Csp6I GTAC 6 cut(s) 550, 1489, 1499, 1579, 1750, 1931
CviAII CATG 8 cut(s) 260, 509, 799, 1187, 1210, 1517, 1685, 1852
CviQI GTAC 6 cut(s) 550, 1489, 1499, 1579, 1750, 1931
DdeI CTNAG 9 cut(s) 149, 830, 858, 988, 1038, 1091, 1160, 1301, 1481
DpnI GATC 7 cut(s) 48, 488, 518, 882, 1136, 1194, 1567
DpnII GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
DraIII CACNNNGTG 1 cut(s) 1822
EaeI YGGCCR 3 cut(s) 78, 393, 1990
Eam1104I CTCTTC 3 cut(s) 97, 238, 1410
EarI CTCTTC 3 cut(s) 97, 238, 1410
EciI GGCGGA 1 cut(s) 1778
Eco130I CCWWGG 2 cut(s) 231, 798
Eco147I AGGCCT 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 674
Eco47I GGWCC 7 cut(s) 118, 285, 435, 446, 1180, 1718, 1741
Eco57I CTGAAG 3 cut(s) 461, 662, 869
Eco72I CACGTG 4 cut(s) 452, 612, 1252, 1388
Eco81I CCTNAGG 1 cut(s) 1038
Eco88I CYCGRG 3 cut(s) 1067, 1151, 1643
EcoO109I RGGNCCY 4 cut(s) 670, 671, 1180, 1741
EcoRII CCWGG 3 cut(s) 281, 677, 1743
EcoT14I CCWWGG 2 cut(s) 231, 798
EcoT22I ATGCAT 1 cut(s) 1686
EcoT38I GRGCYC 1 cut(s) 674
ErhI CCWWGG 2 cut(s) 231, 798
Esp3I CGTCTC 2 cut(s) 728, 1802
FaeI CATG 8 cut(s) 263, 512, 802, 1190, 1213, 1520, 1688, 1855
FaqI GGGAC 6 cut(s) 271, 323, 421, 1193, 1561, 1626
FatI CATG 8 cut(s) 259, 508, 798, 1186, 1209, 1516, 1684, 1851
FauI CCCGC 2 cut(s) 1518, 1567
Fnu4HI GCNGC 8 cut(s) 108, 541, 544, 762, 896, 1019, 1464, 1773
FokI GGATG 7 cut(s) 377, 623, 1187, 1409, 1541, 1615, 1676
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 8 cut(s) 108, 541, 544, 762, 896, 1019, 1464, 1773
FspBI CTAG 4 cut(s) 1271, 1439, 1631, 1733
GlaI GCGC 3 cut(s) 535, 568, 1299
GluI GCNGC 8 cut(s) 108, 541, 544, 762, 896, 1019, 1464, 1773
GsuI CTGGAG 1 cut(s) 1675
HaeII RGCGCY 1 cut(s) 1301
HhaI GCGC 3 cut(s) 536, 569, 1300
Hin1II CATG 8 cut(s) 263, 512, 802, 1190, 1213, 1520, 1688, 1855
Hin6I GCGC 3 cut(s) 534, 567, 1298
HinP1I GCGC 3 cut(s) 534, 567, 1298
HphI GGTGA 5 cut(s) 64, 412, 823, 1066, 1991
Hpy166II GTNNAC 3 cut(s) 420, 449, 774
Hpy188I TCNGA 6 cut(s) 727, 1234, 1377, 1474, 1663, 1722
Hpy8I GTNNAC 3 cut(s) 420, 449, 774
Hpy99I CGWCG 3 cut(s) 731, 1547, 1597
HpyF3I CTNAG 9 cut(s) 149, 830, 858, 988, 1038, 1091, 1160, 1301, 1481
Hsp92II CATG 8 cut(s) 263, 512, 802, 1190, 1213, 1520, 1688, 1855
HspAI GCGC 3 cut(s) 534, 567, 1298
KflI GGGWCCC 1 cut(s) 1180
Kpn2I TCCGGA 1 cut(s) 250
Kzo9I GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 5 cut(s) 132, 631, 1098, 1791, 1825
Lsp1109I GCAGC 4 cut(s) 119, 882, 1005, 1475
LweI GCATC 4 cut(s) 570, 1022, 1784, 1888
MaeI CTAG 4 cut(s) 1271, 1439, 1631, 1733
MaeIII GTNAC 8 cut(s) 601, 607, 786, 1084, 1280, 1321, 1573, 1967
MalI GATC 7 cut(s) 48, 488, 518, 882, 1136, 1194, 1567
MboI GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
MboII GAAGA 8 cut(s) 114, 255, 634, 875, 1069, 1225, 1427, 1650
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 3 cut(s) 636, 674, 1560
MluCI AATT 6 cut(s) 69, 292, 324, 493, 1261, 1697
MlyI GAGTC 8 cut(s) 187, 194, 1171, 1232, 1427, 1502, 1682, 1943
MmeI TCCRAC 3 cut(s) 750, 1270, 1404
Mph1103I ATGCAT 1 cut(s) 1686
MroI TCCGGA 1 cut(s) 250
MseI TTAA 4 cut(s) 356, 720, 825, 2017
MslI CAYNNNNRTG 3 cut(s) 276, 1113, 1805
MspA1I CMGCKG 1 cut(s) 1936
MspR9I CCNGG 9 cut(s) 283, 480, 649, 679, 947, 963, 1001, 1745, 1812
Mva1269I GAATGC 2 cut(s) 10, 1706
MvaI CCWGG 3 cut(s) 283, 679, 1745
MvnI CGCG 2 cut(s) 534, 569
NciI CCSGG 6 cut(s) 480, 649, 947, 963, 1001, 1812
NcoI CCATGG 1 cut(s) 798
NdeII GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
NlaIII CATG 8 cut(s) 263, 512, 802, 1190, 1213, 1520, 1688, 1855
NmeAIII GCCGAG 2 cut(s) 684, 842
NmuCI GTSAC 6 cut(s) 601, 607, 1084, 1280, 1321, 1573
NsiI ATGCAT 1 cut(s) 1686
NspI RCATGY 1 cut(s) 1520
NspV TTCGAA 2 cut(s) 73, 100
OliI CACNNNNGTG 1 cut(s) 1805
PaeR7I CTCGAG 1 cut(s) 1151
PagI TCATGA 2 cut(s) 1209, 1851
PceI AGGCCT 1 cut(s) 597
PciSI GCTCTTC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 2 cut(s) 495, 617
PctI GAATGC 2 cut(s) 10, 1706
PfeI GAWTC 3 cut(s) 844, 1674, 1725
PflFI GACNNNGTC 1 cut(s) 116
PflMI CCANNNNNTGG 3 cut(s) 799, 1252, 1822
PkrI GCNGC 8 cut(s) 109, 542, 545, 763, 897, 1020, 1465, 1774
PleI GAGTC 8 cut(s) 187, 193, 1170, 1231, 1426, 1502, 1682, 1943
PmaCI CACGTG 4 cut(s) 452, 612, 1252, 1388
PmlI CACGTG 4 cut(s) 452, 612, 1252, 1388
PpsI GAGTC 8 cut(s) 187, 193, 1170, 1231, 1426, 1502, 1682, 1943
Ppu21I YACGTR 7 cut(s) 452, 549, 612, 1145, 1252, 1388, 1578
PpuMI RGGWCCY 2 cut(s) 1180, 1741
Psp5II RGGWCCY 2 cut(s) 1180, 1741
Psp6I CCWGG 3 cut(s) 281, 677, 1743
PspCI CACGTG 4 cut(s) 452, 612, 1252, 1388
PspGI CCWGG 3 cut(s) 281, 677, 1743
PspOMI GGGCCC 1 cut(s) 670
PspPPI RGGWCCY 2 cut(s) 1180, 1741
PspXI VCTCGAGB 1 cut(s) 1151
PstI CTGCAG 1 cut(s) 1023
PsuI RGATCY 1 cut(s) 516
PsyI GACNNNGTC 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 1936
RsaI GTAC 6 cut(s) 551, 1490, 1500, 1580, 1751, 1932
RsaNI GTAC 6 cut(s) 550, 1489, 1499, 1579, 1750, 1931
RseI CAYNNNNRTG 3 cut(s) 276, 1113, 1805
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 4 cut(s) 356, 720, 825, 2017
SatI GCNGC 8 cut(s) 108, 541, 544, 762, 896, 1019, 1464, 1773
Sau3AI GATC 7 cut(s) 46, 486, 516, 880, 1134, 1192, 1565
SchI GAGTC 8 cut(s) 187, 194, 1171, 1232, 1427, 1502, 1682, 1943
ScrFI CCNGG 9 cut(s) 283, 480, 649, 679, 947, 963, 1001, 1745, 1812
SduI GDGCHC 3 cut(s) 636, 674, 1560
SfaNI GCATC 4 cut(s) 570, 1022, 1784, 1888
SfcI CTRYAG 1 cut(s) 1019
Sfr274I CTCGAG 1 cut(s) 1151
SfuI TTCGAA 2 cut(s) 73, 100
SinI GGWCC 7 cut(s) 118, 285, 435, 446, 1180, 1718, 1741
SlaI CTCGAG 1 cut(s) 1151
SmiMI CAYNNNNRTG 3 cut(s) 276, 1113, 1805
SmlI CTYRAG 3 cut(s) 848, 1151, 1457
SmoI CTYRAG 3 cut(s) 848, 1151, 1457
Sse9I AATT 6 cut(s) 69, 292, 324, 493, 1261, 1697
SseBI AGGCCT 1 cut(s) 597
SsiI CCGC 9 cut(s) 540, 543, 569, 742, 762, 1525, 1560, 1773, 1789
SspI AATATT 1 cut(s) 919
SspMI CTAG 4 cut(s) 1271, 1439, 1631, 1733
StuI AGGCCT 1 cut(s) 597
StyD4I CCNGG 9 cut(s) 281, 478, 647, 677, 945, 961, 999, 1743, 1810
StyI CCWWGG 2 cut(s) 231, 798
TaqI TCGA 8 cut(s) 73, 100, 223, 489, 654, 996, 1152, 1511
TaqII GACCGA 1 cut(s) 1561
TasI AATT 6 cut(s) 69, 292, 324, 493, 1261, 1697
TatI WGTACW 1 cut(s) 1930
TauI GCSGC 4 cut(s) 543, 546, 764, 1775
TfiI GAWTC 3 cut(s) 844, 1674, 1725
Tru1I TTAA 4 cut(s) 356, 720, 825, 2017
Tru9I TTAA 4 cut(s) 356, 720, 825, 2017
TseFI GTSAC 6 cut(s) 601, 607, 1084, 1280, 1321, 1573
TseI GCWGC 4 cut(s) 107, 895, 1018, 1463
Tsp45I GTSAC 6 cut(s) 601, 607, 1084, 1280, 1321, 1573
TspGWI ACGGA 2 cut(s) 782, 1486
Tth111I GACNNNGTC 1 cut(s) 116
Van91I CCANNNNNTGG 3 cut(s) 799, 1252, 1822
VpaK11BI GGWCC 7 cut(s) 118, 285, 435, 446, 1180, 1718, 1741
XapI RAATTY 2 cut(s) 324, 1697
XceI RCATGY 1 cut(s) 1520
XhoI CTCGAG 1 cut(s) 1151
XspI CTAG 4 cut(s) 1271, 1439, 1631, 1733
Zsp2I ATGCAT 1 cut(s) 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.