Rw1G023090

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013865
Physical Location & Seq
Reverse (-)
48800850 .. 48803296
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023090.1

Sequence Viewer

Length: 1659 bp
ATGGAGATTGGCTCTGCTTTGCTCTTATTCACGGCCATCACGGCTTATCTACTTTGGTTCACCTTCATCTCACGGTCCCTGAAGGGTCCACGTGTTTGGCCCTTACTAGGTAGCCTCCCCGGCTTGATCGAAAATTGCGACCGTTTGCATGACTGGATCTGCGATAACCTACGCGCCTGCGGCGGCACGTACCAAACCTGCATCTGCGCGGTTCCTTTTCTCGCCCAAAAGCAAGGCCTCGTGACCGTCACGTGCGACCCGAAGAATGTGGAGCACATCCTGAAGGCCCGGTTCGACAACTACCCGAAGGGCCCCACCTGGCAATCGGTGTTTCACGATCTTCTCGGCGAGGGGATATTTAACTCCGACGGCGAGACGTGGCGGTTCCAGAGGAAAACTGCCGCCGTGGAGTTTACTACCAGGACGTTGCGCCAAGCCATGGCTAGGTGGGTGAGCCGAGCCATTAAGCTGAGGTTCTGCCCGATTCTCAAGGAAGCTGAGCTGAAAGGCGAGCCGGTAGATCTTCAGGACTTGCTGCTTCGGCTCACTTTTGATAATATTTGTGGCTTGGCTTTCGGGAAAGACCCGGAGACTTGCTCTGCGGGGTATCCCGACAACAGCTTCGCCTCGGCTTTCGACCGGGCCACCGAGGCGTCGCTGCAGCGGTTCATTTTGCCTGAGGTGTTGTGGAAGGTGAAGAAATGGCTCGGGCTTGGAATGGAAGTCACGCTGAGCCGGAGCCTTGTGCATATTGATGAGTATTTATCCAAAGTGATCGCTTCACGTAAGCTCGAGTTGCTGAGTCAGCAGAAGGATGGGAACCCACATGATGATCTGCTGTCAAGGTTCATGAAGAAAAAGGAGTCCTACTCGGACACCTTCCTCCAACACGTGGCGCTCAATTTCATCCTAGCCGGACGTGACACGTCATCGGTGGCGCTCAGCTGGTTCTTTTGGTTAGTGACACTAAACCCAATTATTGAGGAGAAAATCTTGCGAGAAATCTGCACCGTTCTGATTGAGACGCGTGGCGAGGATGTTGCAGCATGGTTAGAAGAGTCGTTGCATTTTGAAGAACTTGACCAATTGATATACCTCAAGGCAGCATTGTCCGAAAGTCTTAGGTTGTACCCTTCCGTACCCCAAGACTCGAAGCATGTTGTGCGGGATGATGTTTTGCCCGACGGGACATTTGTGCCGGCCGGATCATCGGTCACGTACTCAATATACGCGACGGGGAGGATGAAGTCCTCGTGGGGCGACGATTGCCTAGAGTTTCGTCCTGAGAGGTGGTTGTCTTCGGATGGTAAGAGATTCATAATGCATGACTCTAGCAAATATGTAGCTTTCAATGCTGGTCCGAGAATCTGTCTAGGGAAGGACCTGGCGTACCTGCAAATGAAGTCGGTAGCTGCATCGGTACTGCTCCGCCACCGTCTCACGGTGGTGCCGGGCCACAAGGTGGAGCAAAAGATGTCACTGACATTGTTCATGAAGTATGGTTTGATGATGAATGTGCATCAAAGGGATTTAGAGGGTGTTTTGGGAAGGGCAAAGAGAGACAGGGAAGTAAAGCTGAATGGACAAGACTCTGTTGCCGTCAAGTGTAACGGTGTTGGTGAGAACGGTGGTGGTGAAGGGGTAGTTGGGGCTGATTAA

Protein Analysis

552

Amino Acids

62.14

Weight (kDa)

7.16

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 28 - 495 1e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01600 AT2G45970 AT4G00360
fragaria_vesca FvH4_7g14480
malus_domestica MD01G1064900.v1.1 MD07G1135500.v1.1
prunus_persica Prupe.2G171800_v2.0.a1
pyrus_communis pycom01g09420 pycom07g13270
rosa_chinensis RchiOBHm_Chr1g0355891
rosa_laevigata RLG00000028118
rosa_roxburghii Rroxscaffold_4G00299940
rosa_rugosa Rorug01G0248200
rosa_samantha Rh1AG261500 Rh1BG229800 Rh1DG257800
rosa_wichuraiana Rw1G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 206, 1401
AccB1I GGYRCC 1 cut(s) 1447
AccB7I CCANNNNNTGG 3 cut(s) 439, 892, 1462
AccII CGCG 4 cut(s) 174, 209, 1027, 1232
AciI CCGC 9 cut(s) 180, 183, 209, 382, 402, 602, 664, 1165, 1429
AclWI GGATC 2 cut(s) 164, 1213
AcoI YGGCCR 2 cut(s) 33, 1200
AcuI CTGAAG 3 cut(s) 101, 302, 509
AcvI CACGTG 3 cut(s) 92, 252, 892
AcyI GRCGYC 1 cut(s) 653
AdeI CACNNNGTG 1 cut(s) 1462
AfaI GTAC 6 cut(s) 191, 1130, 1140, 1220, 1391, 1422
AfiI CCNNNNNNNGG 6 cut(s) 107, 439, 444, 892, 1441, 1462
AflIII ACRYGT 4 cut(s) 91, 889, 924, 1025
AgsI TTSAA 2 cut(s) 1073, 1351
AjiI CACGTC 3 cut(s) 378, 920, 927
AjnI CCWGG 3 cut(s) 317, 419, 1383
AjuI GAANNNNNNNTTGG 1 cut(s) 1629
AleI CACNNNNGTG 1 cut(s) 1445
AluBI AGCT 9 cut(s) 469, 497, 502, 621, 790, 945, 1346, 1412, 1576
AluI AGCT 9 cut(s) 469, 497, 502, 621, 790, 945, 1346, 1412, 1576
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 5 cut(s) 368, 584, 1016, 1442, 1554
AlwI GGATC 2 cut(s) 164, 1213
Ama87I CYCGRG 2 cut(s) 707, 791
AoxI GGCC 8 cut(s) 33, 98, 235, 285, 310, 642, 1200, 1453
ApaI GGGCCC 1 cut(s) 314
ApeKI GCWGC 6 cut(s) 535, 658, 661, 1043, 1103, 1412
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 1175, 1207
AspLEI GCGC 5 cut(s) 176, 209, 432, 898, 940
AsuC2I CCSGG 5 cut(s) 120, 289, 587, 641, 1452
AsuHPI GGTGA 5 cut(s) 52, 463, 706, 1631, 1646
AvaI CYCGRG 2 cut(s) 707, 791
AvaII GGWCC 4 cut(s) 75, 86, 1358, 1381
AxyI CCTNAGG 1 cut(s) 678
BaeGI GKGCMC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 1447
BanII GRGCYC 1 cut(s) 314
BauI CACGAG 2 cut(s) 239, 1252
BbrPI CACGTG 3 cut(s) 92, 252, 892
BbsI GAAGAC 1 cut(s) 1290
Bbv12I GWGCWC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 470
BbvI GCAGC 6 cut(s) 522, 645, 673, 1055, 1115, 1399
BccI CCATC 3 cut(s) 44, 809, 1298
BceAI ACGGC 5 cut(s) 48, 57, 385, 389, 1583
BcgI CGANNNNNNTGC 4 cut(s) 987, 1021, 1022, 1056
BciT130I CCWGG 3 cut(s) 319, 421, 1385
BciVI GTATCC 1 cut(s) 618
BcnI CCSGG 5 cut(s) 120, 289, 587, 641, 1452
BcoDI GTCTC 5 cut(s) 368, 584, 1016, 1442, 1554
BfaI CTAG 6 cut(s) 107, 444, 911, 1271, 1332, 1373
BfmI CTRYAG 1 cut(s) 659
BfoI RGCGCY 2 cut(s) 899, 941
BfuAI ACCTGC 2 cut(s) 206, 1401
BfuI GTATCC 1 cut(s) 618
BglI GCCNNNNNGGC 3 cut(s) 41, 120, 650
BglII AGATCT 1 cut(s) 520
BisI GCNGC 9 cut(s) 181, 184, 402, 536, 659, 662, 1044, 1104, 1413
BlpI GCTNAGC 3 cut(s) 498, 731, 941
BlsI GCNGC 9 cut(s) 182, 185, 403, 537, 660, 663, 1045, 1105, 1414
Bme1390I CCNGG 8 cut(s) 120, 289, 319, 421, 587, 641, 1385, 1452
Bme18I GGWCC 4 cut(s) 75, 86, 1358, 1381
BmeT110I CYCGRG 2 cut(s) 707, 791
BmgBI CACGTC 3 cut(s) 378, 920, 927
BmiI GGNNCC 9 cut(s) 77, 87, 213, 312, 313, 386, 740, 821, 1449
BmrFI CCNGG 8 cut(s) 120, 289, 319, 421, 587, 641, 1385, 1452
BmsI GCATC 3 cut(s) 210, 1424, 1528
BpiI GAAGAC 1 cut(s) 1290
BplI GAGNNNNNCTC 5 cut(s) 28, 581, 613, 854, 886
Bpu10I CCTNAGC 1 cut(s) 470
Bpu1102I GCTNAGC 3 cut(s) 498, 731, 941
BpuEI CTTGAG 2 cut(s) 473, 1082
BpuMI CCSGG 5 cut(s) 120, 289, 587, 641, 1452
BsaAI YACGTR 6 cut(s) 92, 189, 252, 785, 892, 1218
BsaHI GRCGYC 1 cut(s) 653
BsaJI CCNNGG 5 cut(s) 118, 405, 438, 627, 648
Bsc4I CCNNNNNNNGG 6 cut(s) 107, 439, 444, 892, 1441, 1462
Bse118I RCCGGY 2 cut(s) 514, 1198
Bse1I ACTGG 1 cut(s) 158
Bse21I CCTNAGG 1 cut(s) 678
BseBI CCWGG 3 cut(s) 319, 421, 1385
BseDI CCNNGG 5 cut(s) 118, 405, 438, 627, 648
BseGI GGATG 7 cut(s) 276, 820, 906, 1042, 1174, 1248, 1309
BseLI CCNNNNNNNGG 6 cut(s) 107, 439, 444, 892, 1441, 1462
BseMII CTCAG 7 cut(s) 461, 489, 669, 722, 791, 955, 1275
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 1 cut(s) 998
BseSI GKGCMC 1 cut(s) 314
BseX3I CGGCCG 1 cut(s) 1200
BseXI GCAGC 6 cut(s) 522, 645, 673, 1055, 1115, 1399
BsgI GTGCAG 1 cut(s) 991
Bsh1236I CGCG 4 cut(s) 174, 209, 1027, 1232
Bsh1285I CGRYCG 3 cut(s) 142, 640, 1203
BshFI GGCC 8 cut(s) 35, 100, 237, 287, 312, 644, 1202, 1455
BshNI GGYRCC 1 cut(s) 1447
BsiEI CGRYCG 3 cut(s) 142, 640, 1203
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 707, 791
BslFI GGGAC 2 cut(s) 61, 1201
BslI CCNNNNNNNGG 6 cut(s) 107, 439, 444, 892, 1441, 1462
BsmAI GTCTC 5 cut(s) 368, 584, 1016, 1442, 1554
BsmBI CGTCTC 3 cut(s) 368, 1016, 1442
BsmFI GGGAC 2 cut(s) 61, 1201
BsnI GGCC 8 cut(s) 35, 100, 237, 287, 312, 644, 1202, 1455
BsoBI CYCGRG 2 cut(s) 707, 791
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 276, 314
Bsp143I GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
Bsp1720I GCTNAGC 3 cut(s) 498, 731, 941
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 9 cut(s) 180, 183, 209, 382, 402, 602, 664, 1165, 1429
BspANI GGCC 8 cut(s) 35, 100, 237, 287, 312, 644, 1202, 1455
BspCNI CTCAG 7 cut(s) 462, 490, 670, 723, 792, 954, 1276
BspFNI CGCG 4 cut(s) 174, 209, 1027, 1232
BspHI TCATGA 2 cut(s) 849, 1491
BspLI GGNNCC 9 cut(s) 77, 87, 213, 312, 313, 386, 740, 821, 1449
BspMAI CTGCAG 1 cut(s) 663
BspMI ACCTGC 2 cut(s) 206, 1401
BspPI GGATC 2 cut(s) 164, 1213
BspT107I GGYRCC 1 cut(s) 1447
BsrFI RCCGGY 2 cut(s) 514, 1198
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 2 cut(s) 514, 1198
BssECI CCNNGG 5 cut(s) 118, 405, 438, 627, 648
BssMI GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
BssNI GRCGYC 1 cut(s) 653
BssSI CACGAG 2 cut(s) 239, 1252
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 2 cut(s) 239, 1252
Bst2UI CCWGG 3 cut(s) 319, 421, 1385
Bst4CI ACNGT 8 cut(s) 75, 143, 247, 1012, 1436, 1444, 1613, 1628
Bst6I CTCTTC 1 cut(s) 1050
BstACI GRCGYC 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 1162
BstBAI YACGTR 6 cut(s) 92, 189, 252, 785, 892, 1218
BstC8I GCNNGC 3 cut(s) 178, 512, 1200
BstDEI CTNAG 8 cut(s) 470, 498, 678, 731, 800, 941, 1121, 1284
BstDSI CCRYGG 2 cut(s) 405, 438
BstF5I GGATG 7 cut(s) 276, 820, 906, 1042, 1174, 1248, 1309
BstFNI CGCG 4 cut(s) 174, 209, 1027, 1232
BstH2I RGCGCY 2 cut(s) 899, 941
BstHHI GCGC 5 cut(s) 176, 209, 432, 898, 940
BstKTI GATC 7 cut(s) 129, 159, 340, 523, 777, 835, 1208
BstMAI GTCTC 5 cut(s) 368, 584, 1016, 1442, 1554
BstMBI GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
BstMCI CGRYCG 3 cut(s) 142, 640, 1203
BstNI CCWGG 3 cut(s) 319, 421, 1385
BstNSI RCATGY 1 cut(s) 1160
BstSCI CCNGG 8 cut(s) 118, 287, 317, 419, 585, 639, 1383, 1450
BstSFI CTRYAG 1 cut(s) 659
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 4 cut(s) 174, 209, 1027, 1232
BstV1I GCAGC 6 cut(s) 522, 645, 673, 1055, 1115, 1399
BstV2I GAAGAC 1 cut(s) 1290
BstX2I RGATCY 2 cut(s) 156, 520
BstXI CCANNNNNNTGG 1 cut(s) 96
BstYI RGATCY 2 cut(s) 156, 520
BstZI CGGCCG 1 cut(s) 1200
Bsu36I CCTNAGG 1 cut(s) 678
BsuI GTATCC 1 cut(s) 618
BsuRI GGCC 8 cut(s) 35, 100, 237, 287, 312, 644, 1202, 1455
BtgI CCRYGG 2 cut(s) 405, 438
BtrI CACGTC 3 cut(s) 378, 920, 927
BtsCI GGATG 7 cut(s) 276, 820, 906, 1042, 1174, 1248, 1309
BtsIMutI CAGTG 1 cut(s) 1478
BveI ACCTGC 2 cut(s) 206, 1401
Cac8I GCNNGC 3 cut(s) 178, 512, 1200
CciI TCATGA 2 cut(s) 849, 1491
CfoI GCGC 5 cut(s) 176, 209, 432, 898, 940
Cfr10I RCCGGY 2 cut(s) 514, 1198
CseI GACGC 2 cut(s) 642, 1033
Csp6I GTAC 6 cut(s) 190, 1129, 1139, 1219, 1390, 1421
CviAII CATG 8 cut(s) 149, 439, 827, 850, 1047, 1157, 1325, 1492
CviQI GTAC 6 cut(s) 190, 1129, 1139, 1219, 1390, 1421
DdeI CTNAG 8 cut(s) 470, 498, 678, 731, 800, 941, 1121, 1284
DpnI GATC 7 cut(s) 128, 158, 339, 522, 776, 834, 1207
DpnII GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
DraIII CACNNNGTG 1 cut(s) 1462
EaeI YGGCCR 2 cut(s) 33, 1200
EagI CGGCCG 1 cut(s) 1200
Eam1104I CTCTTC 1 cut(s) 1050
EarI CTCTTC 1 cut(s) 1050
EciI GGCGGA 1 cut(s) 1418
EclXI CGGCCG 1 cut(s) 1200
Eco130I CCWWGG 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 4 cut(s) 75, 86, 1358, 1381
Eco52I CGGCCG 1 cut(s) 1200
Eco57I CTGAAG 3 cut(s) 101, 302, 509
Eco72I CACGTG 3 cut(s) 92, 252, 892
Eco81I CCTNAGG 1 cut(s) 678
Eco88I CYCGRG 2 cut(s) 707, 791
EcoO109I RGGNCCY 3 cut(s) 310, 311, 1381
EcoRII CCWGG 3 cut(s) 317, 419, 1383
EcoT14I CCWWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 1326
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 3 cut(s) 368, 1016, 1442
FaeI CATG 8 cut(s) 152, 442, 830, 853, 1050, 1160, 1328, 1495
FaqI GGGAC 2 cut(s) 61, 1201
FatI CATG 8 cut(s) 148, 438, 826, 849, 1046, 1156, 1324, 1491
FauI CCCGC 2 cut(s) 595, 1158
Fnu4HI GCNGC 9 cut(s) 181, 184, 402, 536, 659, 662, 1044, 1104, 1413
FokI GGATG 7 cut(s) 263, 827, 893, 1049, 1181, 1255, 1316
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 9 cut(s) 181, 184, 402, 536, 659, 662, 1044, 1104, 1413
FspBI CTAG 6 cut(s) 107, 444, 911, 1271, 1332, 1373
GlaI GCGC 5 cut(s) 175, 208, 431, 897, 939
GluI GCNGC 9 cut(s) 181, 184, 402, 536, 659, 662, 1044, 1104, 1413
HaeII RGCGCY 2 cut(s) 899, 941
HaeIII GGCC 8 cut(s) 35, 100, 237, 287, 312, 644, 1202, 1455
HgaI GACGC 2 cut(s) 642, 1033
HhaI GCGC 5 cut(s) 176, 209, 432, 898, 940
Hin1I GRCGYC 1 cut(s) 653
Hin1II CATG 8 cut(s) 152, 442, 830, 853, 1050, 1160, 1328, 1495
Hin6I GCGC 5 cut(s) 174, 207, 430, 896, 938
HinP1I GCGC 5 cut(s) 174, 207, 430, 896, 938
HinfI GANTC 9 cut(s) 484, 802, 863, 1058, 1148, 1314, 1328, 1365, 1589
HphI GGTGA 5 cut(s) 52, 463, 706, 1631, 1646
Hpy166II GTNNAC 3 cut(s) 60, 89, 414
Hpy188I TCNGA 6 cut(s) 367, 874, 1017, 1114, 1303, 1362
Hpy8I GTNNAC 3 cut(s) 60, 89, 414
Hpy99I CGWCG 5 cut(s) 371, 658, 1187, 1237, 1265
HpyCH4III ACNGT 8 cut(s) 75, 143, 247, 1012, 1436, 1444, 1613, 1628
HpyF3I CTNAG 8 cut(s) 470, 498, 678, 731, 800, 941, 1121, 1284
Hsp92I GRCGYC 1 cut(s) 653
Hsp92II CATG 8 cut(s) 152, 442, 830, 853, 1050, 1160, 1328, 1495
HspAI GCGC 5 cut(s) 174, 207, 430, 896, 938
KroI GCCGGC 1 cut(s) 1198
KroNI GCCGGC 1 cut(s) 1200
Kzo9I GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
LmnI GCTCC 4 cut(s) 271, 738, 1431, 1465
Lsp1109I GCAGC 6 cut(s) 522, 645, 673, 1055, 1115, 1399
LweI GCATC 3 cut(s) 210, 1424, 1528
MaeI CTAG 6 cut(s) 107, 444, 911, 1271, 1332, 1373
MaeIII GTNAC 8 cut(s) 241, 247, 724, 920, 961, 1213, 1476, 1607
MalI GATC 7 cut(s) 128, 158, 339, 522, 776, 834, 1207
MboI GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
MboII GAAGA 8 cut(s) 274, 332, 515, 709, 865, 1067, 1085, 1290
MfeI CAATTG 1 cut(s) 1085
MflI RGATCY 2 cut(s) 156, 520
MhlI GDGCHC 2 cut(s) 276, 314
MluCI AATT 4 cut(s) 133, 901, 975, 1085
MluI ACGCGT 1 cut(s) 1025
MlyI GAGTC 6 cut(s) 811, 872, 1067, 1142, 1322, 1583
MmeI TCCRAC 2 cut(s) 390, 910
Mph1103I ATGCAT 1 cut(s) 1326
MroNI GCCGGC 1 cut(s) 1198
MseI TTAA 3 cut(s) 360, 465, 1657
MslI CAYNNNNRTG 2 cut(s) 753, 1445
MspA1I CMGCKG 2 cut(s) 664, 945
MspR9I CCNGG 8 cut(s) 120, 289, 319, 421, 587, 641, 1385, 1452
MunI CAATTG 1 cut(s) 1085
MvaI CCWGG 3 cut(s) 319, 421, 1385
MvnI CGCG 4 cut(s) 174, 209, 1027, 1232
NaeI GCCGGC 1 cut(s) 1200
NciI CCSGG 5 cut(s) 120, 289, 587, 641, 1452
NcoI CCATGG 1 cut(s) 438
NdeII GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
NgoMIV GCCGGC 1 cut(s) 1198
NlaIII CATG 8 cut(s) 152, 442, 830, 853, 1050, 1160, 1328, 1495
NlaIV GGNNCC 9 cut(s) 77, 87, 213, 312, 313, 386, 740, 821, 1449
NmeAIII GCCGAG 3 cut(s) 324, 482, 608
NmuCI GTSAC 7 cut(s) 241, 247, 724, 920, 961, 1213, 1476
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 1 cut(s) 1160
OliI CACNNNNGTG 1 cut(s) 1445
PaeR7I CTCGAG 1 cut(s) 791
PagI TCATGA 2 cut(s) 849, 1491
PceI AGGCCT 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 257
PdiI GCCGGC 1 cut(s) 1200
PfeI GAWTC 3 cut(s) 484, 1314, 1365
PflMI CCANNNNNTGG 3 cut(s) 439, 892, 1462
PkrI GCNGC 9 cut(s) 182, 185, 403, 537, 660, 663, 1045, 1105, 1414
PleI GAGTC 6 cut(s) 810, 871, 1066, 1142, 1322, 1583
PmaCI CACGTG 3 cut(s) 92, 252, 892
PmlI CACGTG 3 cut(s) 92, 252, 892
PpsI GAGTC 6 cut(s) 810, 871, 1066, 1142, 1322, 1583
Ppu21I YACGTR 6 cut(s) 92, 189, 252, 785, 892, 1218
PpuMI RGGWCCY 1 cut(s) 1381
Psp5II RGGWCCY 1 cut(s) 1381
Psp6I CCWGG 3 cut(s) 317, 419, 1383
PspCI CACGTG 3 cut(s) 92, 252, 892
PspGI CCWGG 3 cut(s) 317, 419, 1383
PspN4I GGNNCC 9 cut(s) 77, 87, 213, 312, 313, 386, 740, 821, 1449
PspOMI GGGCCC 1 cut(s) 310
PspPPI RGGWCCY 1 cut(s) 1381
PspXI VCTCGAGB 1 cut(s) 791
PstI CTGCAG 1 cut(s) 663
PsuI RGATCY 2 cut(s) 156, 520
PvuII CAGCTG 1 cut(s) 945
RsaI GTAC 6 cut(s) 191, 1130, 1140, 1220, 1391, 1422
RsaNI GTAC 6 cut(s) 190, 1129, 1139, 1219, 1390, 1421
RseI CAYNNNNRTG 2 cut(s) 753, 1445
SaqAI TTAA 3 cut(s) 360, 465, 1657
SatI GCNGC 9 cut(s) 181, 184, 402, 536, 659, 662, 1044, 1104, 1413
Sau3AI GATC 7 cut(s) 126, 156, 337, 520, 774, 832, 1205
SchI GAGTC 6 cut(s) 811, 872, 1067, 1142, 1322, 1583
ScrFI CCNGG 8 cut(s) 120, 289, 319, 421, 587, 641, 1385, 1452
SduI GDGCHC 2 cut(s) 276, 314
SfaNI GCATC 3 cut(s) 210, 1424, 1528
SfcI CTRYAG 1 cut(s) 659
Sfr274I CTCGAG 1 cut(s) 791
SinI GGWCC 4 cut(s) 75, 86, 1358, 1381
SlaI CTCGAG 1 cut(s) 791
SmiMI CAYNNNNRTG 2 cut(s) 753, 1445
SmlI CTYRAG 3 cut(s) 488, 791, 1097
SmoI CTYRAG 3 cut(s) 488, 791, 1097
Sse9I AATT 4 cut(s) 133, 901, 975, 1085
SseBI AGGCCT 1 cut(s) 237
SsiI CCGC 9 cut(s) 180, 183, 209, 382, 402, 602, 664, 1165, 1429
SspI AATATT 1 cut(s) 559
SspMI CTAG 6 cut(s) 107, 444, 911, 1271, 1332, 1373
StuI AGGCCT 1 cut(s) 237
StyD4I CCNGG 8 cut(s) 118, 287, 317, 419, 585, 639, 1383, 1450
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 8 cut(s) 75, 143, 247, 1012, 1436, 1444, 1613, 1628
TaqI TCGA 5 cut(s) 129, 294, 636, 792, 1151
TaqII GACCGA 1 cut(s) 1201
TasI AATT 4 cut(s) 133, 901, 975, 1085
TauI GCSGC 3 cut(s) 183, 186, 404
TfiI GAWTC 3 cut(s) 484, 1314, 1365
Tru1I TTAA 3 cut(s) 360, 465, 1657
Tru9I TTAA 3 cut(s) 360, 465, 1657
TscAI CASTG 1 cut(s) 1485
TseFI GTSAC 7 cut(s) 241, 247, 724, 920, 961, 1213, 1476
TseI GCWGC 6 cut(s) 535, 658, 661, 1043, 1103, 1412
Tsp45I GTSAC 7 cut(s) 241, 247, 724, 920, 961, 1213, 1476
TspGWI ACGGA 1 cut(s) 1126
TspRI CASTG 1 cut(s) 1485
Van91I CCANNNNNTGG 3 cut(s) 439, 892, 1462
VpaK11BI GGWCC 4 cut(s) 75, 86, 1358, 1381
XceI RCATGY 1 cut(s) 1160
XhoI CTCGAG 1 cut(s) 791
XspI CTAG 6 cut(s) 107, 444, 911, 1271, 1332, 1373
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.