Rorug01G0248200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013865
Physical Location & Seq
Forward (+)
35609967 .. 35614602
4636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0248200.1

Sequence Viewer

Length: 1734 bp
ATGGCTTTCTGGGGAATTGAAGTGAAACCCGGAAAGCCTTTTACTCATAAACCTGATGACTCCAATGGACGGCTTCACATTTCAATGGCAACATTGGGGTTGGGGGCTGCAACCAAAAGAAGCATACTCCAGTGTAATGTAGGTAACAAGAGTCCGGTTTATCTATGCGCGTTGTATCCTGAGAAGATCGAGTGTTTGCAACTACATTTGGAATTTGACGAGGCTGATGAAGTCATGTTCTCAGTTCTTGGTCCTCGGAGTGTTCACCTTGCTGGTTACTATCTAGGTGGTCGTCGGCCTTACAATCCAGATGAGTCAGAATCCTTTGGAGAAGATATTGCTGACTCAGAGACACAGAGATCTGTTAACAGTGAAGACGAAAAGTATGATGACAGTTTTATTGATGATGATGATCTTGAAGTCTTCCCATCTACTCCTGCATCTGATGCTACAGATTCTGATTCTTGTTCTGAAGAGGAGTTTTTGGCTAAGAAGAAACCTCAGAAAGAGAAGGCACGGCACAGACAACTTAGAAAGACGTACGTAATAAGTGATTCAGAAGTTGAAAGTGAAAATGGTGATGACGACAATCACCCAATTTCCTCTTTGGTTAAGACAAAATCATCCACTGTTGAGAATGCAATACAGGAAATGGAAGAAAAAGTTGACAAGGGAACTGTTGATACCAGCAACAAGAAGGCAGTGGATGATTTTGCTACTGAATCAATTAGACATGCTGATGATGTACTTGTAGATGGTCAGTTGAAGAGGCAATTGGACCTGCCAGTTGATCTGTCTTCTAAAGTAGACCTTGAAAATGGGGGAAAGCCAAAAAGAAAAAGGAAAGAATGGCTGCAGGAGGAGAAAACAGTTGAAGCTGATGTCCTTAATAAAGAGGATGAAGCCCAGAAAAATGAGGCAAAAGCTGAAAATAGGAACCAGAATGGACAACTGATGGCAGTAAACCATAGCGGAGCTGAAATGCCAGATAACTTATCTTTGCCTTCCGCGGAGGTTGGCCTTGGAAATAGTGATAAACCAAAGAAGAAAAGGAAGAAGCGGGTGGAGGAGAAAACAGCTGAGGTTATCTGTACTAACCCTGGTAATGATATAAAAGAGGATGAGGCAAAGGGTGACTTCTGCTTGGATCCACCACTCAAAAGTGAACAAAATCAGAAGTCAGCCAAGGATGAGGGTTTTGGCCATGACTCCCATAAACTTGTTGATGAACCTGATGAAAAGAAAGTCAAAAAGAAGAAGAAGAAGAAAAGCAAAACCCAATCACATGGAGAAACCGTGAACACAGATATAGCTCTCTTGTCAGTAGAAGAGAAGGTCACATCTCCCGTGGAGAATGAGGCCAATTATGTCGATGCTAAATCATCTGAAGTTAGAACCGTCCCCAGTGGATTACACATTGAGGAGTTAGAAGCAGGAGAACCAAATGGCAAAGTTGCAACCTCTGGGAAAAAGATCACTGTTCATTATGTTGGCAAGTTGAAAAAGAATGGAGAAGTAGTTGACTCTTGTGATGGTGGTCCTCCTTCTAAGTTCCGCTTAGGTCGGGGAAAAGTTATGGAGGGTTTGGATGCTGGTATTGATGGTATGCGAGTCGGTGGCAAACGAAGACTTGTTATCCCACCTTCAATGGCTTATGGGAGCAAAGGAGATGATAAAGATATACCACCAGACTCATGGCTGGTATACGATGTTGAGTTGGTGAAAGTTCAGTGA

Protein Analysis

577

Amino Acids

63.73

Weight (kDa)

5.18

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPL PF17800 3 - 94 3.2e-23 Nucleoplasmin-like domain
FKBP_C PF00254 486 - 574 5.3e-28 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01600 AT2G45970 AT4G00360
fragaria_vesca FvH4_7g14480
malus_domestica MD01G1064900.v1.1 MD07G1135500.v1.1
prunus_persica Prupe.2G171800_v2.0.a1
pyrus_communis pycom01g09420 pycom07g13270
rosa_chinensis RchiOBHm_Chr1g0355891
rosa_laevigata RLG00000028118
rosa_roxburghii Rroxscaffold_4G00299940
rosa_rugosa Rorug01G0248200
rosa_samantha Rh1AG261500 Rh1BG229800 Rh1DG257800
rosa_wichuraiana Rw1G023090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 789
AccI GTMKAC 2 cut(s) 807, 1704
AccII CGCG 2 cut(s) 170, 1010
AciI CCGC 5 cut(s) 972, 1008, 1010, 1060, 1555
AclWI GGATC 2 cut(s) 1142, 1155
AcoI YGGCCR 1 cut(s) 1201
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 2 cut(s) 492, 1407
AfaI GTAC 3 cut(s) 542, 747, 1093
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 9 cut(s) 20, 84, 419, 566, 766, 815, 875, 1501, 1647
AjnI CCWGG 1 cut(s) 1099
AjuI GAANNNNNNNTTGG 2 cut(s) 758, 790
AluBI AGCT 5 cut(s) 878, 926, 977, 1079, 1313
AluI AGCT 5 cut(s) 878, 926, 977, 1079, 1313
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 2 cut(s) 1142, 1155
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 4 cut(s) 296, 1018, 1201, 1359
ApeKI GCWGC 2 cut(s) 107, 853
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 3 cut(s) 251, 778, 1538
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 5 cut(s) 257, 584, 590, 1145, 1732
AvaII GGWCC 3 cut(s) 251, 778, 1538
BalI TGGCCA 1 cut(s) 1203
BamHI GGATCC 1 cut(s) 1147
BbsI GAAGAC 4 cut(s) 381, 415, 789, 1633
BbvCI CCTCAGC 1 cut(s) 1080
BbvI GCAGC 2 cut(s) 94, 840
BccI CCATC 5 cut(s) 436, 749, 949, 1526, 1595
BceAI ACGGC 2 cut(s) 86, 533
BciT130I CCWGG 1 cut(s) 1101
BciVI GTATCC 1 cut(s) 186
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 2 cut(s) 450, 854
BfuAI ACCTGC 1 cut(s) 789
BfuI GTATCC 1 cut(s) 186
BglII AGATCT 1 cut(s) 359
BisI GCNGC 2 cut(s) 108, 854
BlsI GCNGC 2 cut(s) 109, 855
Bme1390I CCNGG 2 cut(s) 30, 1101
Bme18I GGWCC 3 cut(s) 251, 778, 1538
BmgT120I GGNCC 3 cut(s) 251, 778, 1538
BmiI GGNNCC 2 cut(s) 938, 1149
BmrFI CCNGG 2 cut(s) 30, 1101
BmrI ACTGGG 1 cut(s) 1398
BmsI GCATC 4 cut(s) 436, 449, 1363, 1579
BmuI ACTGGG 1 cut(s) 1398
BpiI GAAGAC 4 cut(s) 381, 415, 789, 1633
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 2 cut(s) 1080, 1558
BpuMI CCSGG 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 544
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 6 cut(s) 254, 1008, 1021, 1099, 1185, 1347
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 4 cut(s) 1281, 1311, 1503, 1533
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 3 cut(s) 130, 785, 1404
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 1 cut(s) 1101
BseDI CCNNGG 6 cut(s) 254, 1008, 1021, 1099, 1185, 1347
BseGI GGATG 6 cut(s) 623, 712, 904, 1126, 1195, 1594
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 5 cut(s) 171, 255, 360, 515, 1071
BseNI ACTGG 3 cut(s) 130, 785, 1404
BseRI GAGGAG 4 cut(s) 491, 875, 1082, 1436
BseXI GCAGC 2 cut(s) 94, 840
Bsh1236I CGCG 2 cut(s) 170, 1010
BshFI GGCC 4 cut(s) 298, 1020, 1203, 1361
BsiSI CCGG 2 cut(s) 30, 155
BsiWI CGTACG 1 cut(s) 540
BslFI GGGAC 1 cut(s) 1385
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 1385
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 4 cut(s) 298, 1020, 1203, 1361
Bsp143I GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
BspACI CCGC 5 cut(s) 972, 1008, 1010, 1060, 1555
BspANI GGCC 4 cut(s) 298, 1020, 1203, 1361
BspCNI CTCAG 5 cut(s) 172, 254, 359, 514, 1072
BspFNI CGCG 2 cut(s) 170, 1010
BspLI GGNNCC 2 cut(s) 938, 1149
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 2 cut(s) 1142, 1155
BsrI ACTGG 3 cut(s) 130, 785, 1404
BssECI CCNNGG 6 cut(s) 254, 1008, 1021, 1099, 1185, 1347
BssMI GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
BssNAI GTATAC 1 cut(s) 1705
BssT1I CCWWGG 2 cut(s) 1021, 1185
Bst1107I GTATAC 1 cut(s) 1705
Bst2UI CCWGG 1 cut(s) 1101
Bst4CI ACNGT 8 cut(s) 371, 395, 631, 679, 871, 1297, 1399, 1480
Bst6I CTCTTC 3 cut(s) 468, 761, 1323
BstAPI GCANNNNNTGC 1 cut(s) 446
BstBAI YACGTR 1 cut(s) 544
BstDEI CTNAG 9 cut(s) 180, 241, 346, 489, 501, 530, 1080, 1548, 1558
BstDSI CCRYGG 2 cut(s) 1008, 1347
BstF5I GGATG 6 cut(s) 623, 712, 904, 1126, 1195, 1594
BstFNI CGCG 2 cut(s) 170, 1010
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 6 cut(s) 189, 362, 415, 793, 1150, 1476
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 1 cut(s) 1101
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 2 cut(s) 28, 1099
BstSFI CTRYAG 2 cut(s) 450, 854
BstSNI TACGTA 1 cut(s) 544
BstUI CGCG 2 cut(s) 170, 1010
BstV1I GCAGC 2 cut(s) 94, 840
BstV2I GAAGAC 4 cut(s) 381, 415, 789, 1633
BstX2I RGATCY 2 cut(s) 359, 1147
BstXI CCANNNNNNTGG 2 cut(s) 1286, 1695
BstYI RGATCY 2 cut(s) 359, 1147
BstZ17I GTATAC 1 cut(s) 1705
BsuI GTATCC 1 cut(s) 186
BsuRI GGCC 4 cut(s) 298, 1020, 1203, 1361
BtgI CCRYGG 2 cut(s) 1008, 1347
BtsCI GGATG 6 cut(s) 623, 712, 904, 1126, 1195, 1594
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 6 cut(s) 137, 376, 627, 708, 1411, 1476
BveI ACCTGC 1 cut(s) 789
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 3 cut(s) 251, 778, 1538
Cfr42I CCGCGG 1 cut(s) 1011
Csp6I GTAC 3 cut(s) 541, 746, 1092
CviAII CATG 5 cut(s) 235, 734, 1205, 1286, 1695
CviQI GTAC 3 cut(s) 541, 746, 1092
DdeI CTNAG 9 cut(s) 180, 241, 346, 489, 501, 530, 1080, 1548, 1558
DpnI GATC 6 cut(s) 188, 361, 414, 792, 1149, 1475
DpnII GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
EaeI YGGCCR 1 cut(s) 1201
Eam1104I CTCTTC 3 cut(s) 468, 761, 1323
EarI CTCTTC 3 cut(s) 468, 761, 1323
Eco105I TACGTA 1 cut(s) 544
Eco130I CCWWGG 2 cut(s) 1021, 1185
Eco47I GGWCC 3 cut(s) 251, 778, 1538
Eco57I CTGAAG 2 cut(s) 492, 1407
EcoRII CCWGG 1 cut(s) 1099
EcoT14I CCWWGG 2 cut(s) 1021, 1185
ErhI CCWWGG 2 cut(s) 1021, 1185
FaeI CATG 5 cut(s) 238, 737, 1208, 1289, 1698
FalI AAGNNNNNCTT 6 cut(s) 795, 827, 1121, 1153, 1541, 1573
FaqI GGGAC 1 cut(s) 1385
FatI CATG 5 cut(s) 234, 733, 1204, 1285, 1694
FauI CCCGC 1 cut(s) 1053
FblI GTMKAC 2 cut(s) 807, 1704
Fnu4HI GCNGC 2 cut(s) 108, 854
FokI GGATG 6 cut(s) 610, 719, 911, 1133, 1202, 1601
Fsp4HI GCNGC 2 cut(s) 108, 854
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 169
GluI GCNGC 2 cut(s) 108, 854
GsuI CTGGAG 1 cut(s) 113
HaeIII GGCC 4 cut(s) 298, 1020, 1203, 1361
HapII CCGG 2 cut(s) 30, 155
HhaI GCGC 1 cut(s) 170
Hin1II CATG 5 cut(s) 238, 737, 1208, 1289, 1698
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 3 cut(s) 367, 667, 1522
HindII GTYRAC 3 cut(s) 367, 667, 1522
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 2 cut(s) 30, 155
HphI GGTGA 5 cut(s) 257, 584, 590, 1145, 1732
Hpy166II GTNNAC 9 cut(s) 265, 367, 667, 808, 964, 1166, 1300, 1522, 1705
Hpy188III TCNNGA 3 cut(s) 179, 308, 416
Hpy8I GTNNAC 9 cut(s) 265, 367, 667, 808, 964, 1166, 1300, 1522, 1705
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 6 cut(s) 505, 691, 1014, 1327, 1554, 1653
HpyCH4III ACNGT 8 cut(s) 371, 395, 631, 679, 871, 1297, 1399, 1480
HpyCH4IV ACGT 2 cut(s) 539, 543
HpyCH4V TGCA 6 cut(s) 110, 199, 440, 641, 856, 1457
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 9 cut(s) 180, 241, 346, 489, 501, 530, 1080, 1548, 1558
HpySE526I ACGT 2 cut(s) 539, 543
Hsp92II CATG 5 cut(s) 238, 737, 1208, 1289, 1698
HspAI GCGC 1 cut(s) 168
KspAI GTTAAC 1 cut(s) 367
KspI CCGCGG 1 cut(s) 1011
Kzo9I GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
LmnI GCTCC 2 cut(s) 974, 1659
Lsp1109I GCAGC 2 cut(s) 94, 840
LweI GCATC 4 cut(s) 436, 449, 1363, 1579
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 539, 543
MaeIII GTNAC 4 cut(s) 143, 275, 1133, 1336
MalI GATC 6 cut(s) 188, 361, 414, 792, 1149, 1475
MboI GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
MfeI CAATTG 1 cut(s) 773
MflI RGATCY 2 cut(s) 359, 1147
MlsI TGGCCA 1 cut(s) 1203
MluCI AATT 6 cut(s) 15, 212, 597, 726, 773, 1363
MluNI TGGCCA 1 cut(s) 1203
MlyI GAGTC 8 cut(s) 53, 160, 323, 338, 1202, 1517, 1620, 1685
Mox20I TGGCCA 1 cut(s) 1203
MscI TGGCCA 1 cut(s) 1203
MseI TTAA 3 cut(s) 366, 612, 888
MslI CAYNNNNRTG 2 cut(s) 83, 738
Msp20I TGGCCA 1 cut(s) 1203
MspA1I CMGCKG 2 cut(s) 1010, 1079
MspI CCGG 2 cut(s) 30, 155
MspR9I CCNGG 2 cut(s) 30, 1101
MunI CAATTG 1 cut(s) 773
Mva1269I GAATGC 1 cut(s) 643
MvaI CCWGG 1 cut(s) 1101
MvnI CGCG 2 cut(s) 170, 1010
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 1 cut(s) 30
NdeII GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
NlaIII CATG 5 cut(s) 238, 737, 1208, 1289, 1698
NlaIV GGNNCC 2 cut(s) 938, 1149
NmuCI GTSAC 2 cut(s) 1133, 1336
NspI RCATGY 1 cut(s) 737
PctI GAATGC 1 cut(s) 643
PfeI GAWTC 5 cut(s) 320, 455, 461, 554, 722
Pfl23II CGTACG 1 cut(s) 540
PkrI GCNGC 2 cut(s) 109, 855
PleI GAGTC 8 cut(s) 53, 159, 322, 338, 1202, 1517, 1619, 1685
PpsI GAGTC 8 cut(s) 53, 159, 322, 338, 1202, 1517, 1619, 1685
Ppu21I YACGTR 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 1099
PspGI CCWGG 1 cut(s) 1099
PspLI CGTACG 1 cut(s) 540
PspN4I GGNNCC 2 cut(s) 938, 1149
PspPI GGNCC 3 cut(s) 251, 778, 1538
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
PstI CTGCAG 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 2 cut(s) 359, 1147
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 3 cut(s) 542, 747, 1093
RsaNI GTAC 3 cut(s) 541, 746, 1092
RseI CAYNNNNRTG 2 cut(s) 83, 738
SacII CCGCGG 1 cut(s) 1011
SaqAI TTAA 3 cut(s) 366, 612, 888
SatI GCNGC 2 cut(s) 108, 854
Sau3AI GATC 6 cut(s) 186, 359, 412, 790, 1147, 1473
Sau96I GGNCC 3 cut(s) 251, 778, 1538
SchI GAGTC 8 cut(s) 53, 160, 323, 338, 1202, 1517, 1620, 1685
ScrFI CCNGG 2 cut(s) 30, 1101
SfaNI GCATC 4 cut(s) 436, 449, 1363, 1579
SfcI CTRYAG 2 cut(s) 450, 854
Sfr303I CCGCGG 1 cut(s) 1011
SgrBI CCGCGG 1 cut(s) 1011
SinI GGWCC 3 cut(s) 251, 778, 1538
SmiMI CAYNNNNRTG 2 cut(s) 83, 738
SnaBI TACGTA 1 cut(s) 544
Sse9I AATT 6 cut(s) 15, 212, 597, 726, 773, 1363
SsiI CCGC 5 cut(s) 972, 1008, 1010, 1060, 1555
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 28, 1099
StyI CCWWGG 2 cut(s) 1021, 1185
TaaI ACNGT 8 cut(s) 371, 395, 631, 679, 871, 1297, 1399, 1480
TaiI ACGT 2 cut(s) 542, 546
TaqI TCGA 2 cut(s) 189, 1371
TasI AATT 6 cut(s) 15, 212, 597, 726, 773, 1363
TatI WGTACW 2 cut(s) 745, 1091
TfiI GAWTC 5 cut(s) 320, 455, 461, 554, 722
Tru1I TTAA 3 cut(s) 366, 612, 888
Tru9I TTAA 3 cut(s) 366, 612, 888
TscAI CASTG 6 cut(s) 137, 376, 634, 708, 1411, 1483
TseFI GTSAC 2 cut(s) 1133, 1336
TseI GCWGC 2 cut(s) 107, 853
Tsp45I GTSAC 2 cut(s) 1133, 1336
TspDTI ATGAA 5 cut(s) 243, 915, 1242, 1251, 1472
TspRI CASTG 6 cut(s) 137, 376, 634, 708, 1411, 1483
VpaK11BI GGWCC 3 cut(s) 251, 778, 1538
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 1692
XmiI GTMKAC 2 cut(s) 807, 1704
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.