pycom01g11060

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014369
Physical Location & Seq
Forward (+)
11908749 .. 11912283
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1470 bp
ATGGCTCGGCTCTTCCGATCCAAATCTTGCCATCTTGTTGGACTCACTGAGTTCAACATTCCCCCTCCTAGTCCATTCTCCTACCACCACAACACCAGCAAAGAAGAAGAAGAAGATGATGAAGGGCTGGAATATGAGGAGTTGGATTTTGAAGACGAGGAGGAGGCTGAAGGAAATCCCATGTCTACCCCATTTCTGAGTCCCACGATTAGAGCCAGTGGGAACAACAAAGGTCGAGACTTCCATGGGCATAACGGTCAACAACAGCAGCACCACCAGTCCGCCATACTCGACATTTTTGTGGCGGCTCTGAGGAAGTCTCTGGTAACTTGCAGTGTGGAGAGGGATGACGTGGCGTCCTCTATCGACATCAGTTCTCCGAGCGAGGTGAGACACGTTTCGCATGTCACTTTTGATAGGTTTAATGGGTTTCTGGGTTTGCCCACTGAGCTCGAGCCCGAGGTCCCCAGGAAGGTGCCAAGTGCCAGTGCAAGTGTGTTTGGGGTCTCTGCCAAGTCGATGCAGTGTTCTTATGATAATAATGGGAACAGTGTGCCAACTATACTTCTTATGATGCAAGAACGATTGTATTCAGGAGGAGGCCTTAAAGCAGAAGGAATATTCAGAATAAATGCTGAAAACGGTCAAGAAGAGCATCTTCGTGACCAGTTGAACAAAGGCATCGTGCCACATGGAATTGATGTTCATTGTATAGCAGGTTTGATAAAGGCATGGTTTAGAGAGCTTCCTACAAGGGTACTTGATTCGCTCACACCAGAACAGGTGATGCGCTGCAACACAGAAGATGACTGCACAGAACTTGTGAAGACACTTCCTCCAACAGAAGCTTCACTGCTAGATTGGGCCATCAATTTGATGGCAGATGTTGCGCAGAACGAACAGCACAACAAGATGAATGCCCGCAACATTGCTATGGTTTTTGCACCTAACATGACCGAGATGGCCGATCCCTTGACTGCACTAATACATGCGGTGCAAGTTATGAATTTCCTCAAGACATTGATTTTAAAGATCCTGCGCACGAGAGAGGAATCAGCTGCGGAGGCTACATTGCACTCATCGTGCAAAAAATCTCTTAACGACAACAGACAAACCTTGTATTCGATTATGGGCGATTCTTTTGTTCATGATAGCTCATTCAGAACCGCCACTTTGGACAGCCCAAAAGCTGACATCGATGAGAAACTTTGGTGCTCTCCGGTAATGACTGATGGGGAAGATGAACTCGAATCCACTTCGCACAGCAGCAGCGCATCTACTAAGTGTGAACTTGAGTGTTCAGAAAACGGATGTAGAAGTGGATACGAAGCTGGAGACTGGTTAAGTTGGAGGAAAGGAGTGCGGAAGCTATACAGCCACCCGGTGTCTCAGTTGAGTAAGCCGGCTAAGAAGGGTACGAAACTTGAGATTGTAAATACTAGGGGAGAAGGTGGAGAAGCATGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0008150 GO:0009505 GO:0009531 GO:0009664 GO:0009987 GO:0016020 GO:0016043 GO:0030312 GO:0044464 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

490

Amino Acids

54.28

Weight (kDa)

5.16

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 186 - 320 1.4e-23 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014369)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46710
fragaria_vesca FvH4_7g17360
malus_domestica MD01G1086700.v1.1 MD07G1156800.v1.1
prunus_persica Prupe.2G195100_v2.0.a1
pyrus_communis pycom01g11060 pycom07g15340
rosa_chinensis RchiOBHm_Chr1g0360801
rosa_laevigata RLG00000027785
rosa_multiflora Rmu_ssc0000022.1_g000034
rosa_roxburghii Rroxscaffold_4G00295800
rosa_rugosa Rorug01G0281600
rosa_samantha Rh1AG293600 Rh1BG258200 Rh1CG276100 Rh1DG288500
rosa_wichuraiana Rw1G025980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 891, 1040
Acc36I ACCTGC 1 cut(s) 707
AccB1I GGYRCC 1 cut(s) 475
AccI GTMKAC 1 cut(s) 185
AciI CCGC 7 cut(s) 282, 305, 922, 992, 1061, 1167, 1363
AclWI GGATC 3 cut(s) 12, 962, 1027
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 1 cut(s) 1006
AcuI CTGAAG 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 356
AdeI CACNNNGTG 1 cut(s) 1384
AfaI GTAC 2 cut(s) 759, 1417
AfiI CCNNNNNNNGG 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 3 cut(s) 55, 152, 673
AhdI GACNNNNNGTC 1 cut(s) 355
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 1 cut(s) 467
AjuI GAANNNNNNNTTGG 2 cut(s) 832, 864
AloI GAACNNNNNNTCC 2 cut(s) 687, 719
AluBI AGCT 8 cut(s) 451, 745, 848, 1058, 1155, 1190, 1331, 1369
AluI AGCT 8 cut(s) 451, 745, 848, 1058, 1155, 1190, 1331, 1369
Alw21I GWGCWC 2 cut(s) 453, 1217
Alw26I GTCTC 6 cut(s) 231, 324, 385, 511, 1329, 1392
AlwI GGATC 3 cut(s) 12, 962, 1027
Ama87I CYCGRG 2 cut(s) 452, 458
AoxI GGCC 3 cut(s) 601, 864, 963
ApeKI GCWGC 5 cut(s) 268, 792, 1058, 1266, 1269
ApoI RAATTY 1 cut(s) 1006
AspLEI GCGC 4 cut(s) 792, 892, 1041, 1274
AspS9I GGNCC 2 cut(s) 463, 864
AsuC2I CCSGG 1 cut(s) 1382
AsuHPI GGTGA 2 cut(s) 400, 796
AvaI CYCGRG 2 cut(s) 452, 458
AvaII GGWCC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 2 cut(s) 453, 459
BauI CACGAG 1 cut(s) 1042
BbsI GAAGAC 2 cut(s) 159, 833
Bbv12I GWGCWC 2 cut(s) 453, 1217
BbvI GCAGC 5 cut(s) 280, 779, 1045, 1278, 1281
BccI CCATC 5 cut(s) 39, 871, 875, 955, 1226
BciT130I CCWGG 1 cut(s) 469
BciVI GTATCC 1 cut(s) 1316
BcnI CCSGG 1 cut(s) 1382
BcoDI GTCTC 6 cut(s) 231, 324, 385, 511, 1329, 1392
BfaI CTAG 3 cut(s) 69, 857, 1440
BfuAI ACCTGC 1 cut(s) 707
BfuI GTATCC 1 cut(s) 1316
BisI GCNGC 6 cut(s) 269, 306, 793, 1059, 1267, 1270
BlsI GCNGC 6 cut(s) 270, 307, 794, 1060, 1268, 1271
Bme1390I CCNGG 2 cut(s) 469, 1382
Bme18I GGWCC 1 cut(s) 463
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 2 cut(s) 452, 458
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 463, 864
BmiI GGNNCC 2 cut(s) 465, 477
BmrFI CCNGG 2 cut(s) 469, 1382
BmsI GCATC 6 cut(s) 510, 564, 664, 690, 777, 1283
BpiI GAAGAC 2 cut(s) 159, 833
BplI GAGNNNNNCTC 2 cut(s) 304, 336
BpmI CTGGAG 1 cut(s) 1353
BpuEI CTTGAG 3 cut(s) 998, 1313, 1445
BpuMI CCSGG 1 cut(s) 1382
Bsa29I ATCGAT 1 cut(s) 1197
BsaBI GATNNNNATC 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 356
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 244, 459, 467
BsaWI WCCGGW 1 cut(s) 1219
BsaXI ACNNNNNCTCC 2 cut(s) 820, 850
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse118I RCCGGY 1 cut(s) 1402
Bse1I ACTGG 5 cut(s) 216, 277, 486, 667, 1343
Bse3DI GCAATG 2 cut(s) 927, 1070
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 1 cut(s) 469
BseCI ATCGAT 1 cut(s) 1197
BseDI CCNNGG 3 cut(s) 244, 459, 467
BseGI GGATG 2 cut(s) 352, 1316
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 472
BseMI GCAATG 2 cut(s) 927, 1070
BseMII CTCAG 5 cut(s) 39, 188, 302, 438, 1403
BseNI ACTGG 5 cut(s) 216, 277, 486, 667, 1343
BseRI GAGGAG 4 cut(s) 152, 173, 176, 612
BseXI GCAGC 5 cut(s) 280, 779, 1045, 1278, 1281
BsgI GTGCAG 2 cut(s) 796, 963
BshFI GGCC 3 cut(s) 603, 866, 965
BshNI GGYRCC 1 cut(s) 475
BshVI ATCGAT 1 cut(s) 1197
BsiHKAI GWGCWC 2 cut(s) 453, 1217
BsiHKCI CYCGRG 2 cut(s) 452, 458
BsiSI CCGG 3 cut(s) 1220, 1382, 1403
BslFI GGGAC 2 cut(s) 186, 449
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 6 cut(s) 231, 324, 385, 511, 1329, 1392
BsmFI GGGAC 2 cut(s) 186, 449
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 3 cut(s) 603, 866, 965
Bso31I GGTCTC 1 cut(s) 511
BsoBI CYCGRG 2 cut(s) 452, 458
Bsp1286I GDGCHC 3 cut(s) 453, 459, 1217
Bsp143I GATC 3 cut(s) 17, 967, 1032
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 7 cut(s) 282, 305, 922, 992, 1061, 1167, 1363
BspANI GGCC 3 cut(s) 603, 866, 965
BspCNI CTCAG 5 cut(s) 40, 189, 303, 439, 1402
BspDI ATCGAT 1 cut(s) 1197
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 2 cut(s) 465, 477
BspMI ACCTGC 1 cut(s) 707
BspPI GGATC 3 cut(s) 12, 962, 1027
BspQI GCTCTTC 2 cut(s) 17, 645
BspT107I GGYRCC 1 cut(s) 475
BspTNI GGTCTC 1 cut(s) 511
BsrDI GCAATG 2 cut(s) 927, 1070
BsrFI RCCGGY 1 cut(s) 1402
BsrI ACTGG 5 cut(s) 216, 277, 486, 667, 1343
BssAI RCCGGY 1 cut(s) 1402
BssECI CCNNGG 3 cut(s) 244, 459, 467
BssMI GATC 3 cut(s) 17, 967, 1032
BssNI GRCGYC 1 cut(s) 356
BssSI CACGAG 1 cut(s) 1042
BssT1I CCWWGG 1 cut(s) 244
Bst2BI CACGAG 1 cut(s) 1042
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 3 cut(s) 257, 551, 644
Bst6I CTCTTC 2 cut(s) 17, 645
BstACI GRCGYC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 922, 1404
BstDEI CTNAG 7 cut(s) 48, 197, 311, 447, 1281, 1389, 1407
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 352, 1316
BstHHI GCGC 4 cut(s) 792, 892, 1041, 1274
BstKTI GATC 3 cut(s) 20, 970, 1035
BstMAI GTCTC 6 cut(s) 231, 324, 385, 511, 1329, 1392
BstMBI GATC 3 cut(s) 17, 967, 1032
BstMWI GCNNNNNNNGC 3 cut(s) 448, 887, 1064
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 2 cut(s) 407, 992
BstSCI CCNGG 2 cut(s) 467, 1380
BstV1I GCAGC 5 cut(s) 280, 779, 1045, 1278, 1281
BstV2I GAAGAC 2 cut(s) 159, 833
BstX2I RGATCY 1 cut(s) 1032
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 1 cut(s) 1032
Bsu15I ATCGAT 1 cut(s) 1197
BsuI GTATCC 1 cut(s) 1316
BsuRI GGCC 3 cut(s) 603, 866, 965
BsuTUI ATCGAT 1 cut(s) 1197
BtgI CCRYGG 1 cut(s) 244
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 2 cut(s) 352, 1316
BtsI GCAGTG 3 cut(s) 340, 530, 851
BtsIMutI CAGTG 8 cut(s) 45, 223, 340, 444, 493, 530, 556, 851
BveI ACCTGC 1 cut(s) 707
Cac8I GCNNGC 2 cut(s) 922, 1404
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 4 cut(s) 792, 892, 1041, 1274
Cfr10I RCCGGY 1 cut(s) 1402
Cfr13I GGNCC 2 cut(s) 463, 864
ClaI ATCGAT 1 cut(s) 1197
CseI GACGC 1 cut(s) 345
Csp6I GTAC 2 cut(s) 758, 1416
CviAII CATG 9 cut(s) 181, 245, 404, 692, 732, 952, 989, 1148, 1461
CviQI GTAC 2 cut(s) 758, 1416
DdeI CTNAG 7 cut(s) 48, 197, 311, 447, 1281, 1389, 1407
DpnI GATC 3 cut(s) 19, 969, 1034
DpnII GATC 3 cut(s) 17, 967, 1032
DraI TTTAAA 1 cut(s) 1029
DraIII CACNNNGTG 1 cut(s) 1384
DriI GACNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 2 cut(s) 17, 645
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 2 cut(s) 17, 645
EciI GGCGGA 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 603
Eco24I GRGCYC 2 cut(s) 453, 459
Eco31I GGTCTC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 452, 458
EcoICRI GAGCTC 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 2 cut(s) 453, 459
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 9 cut(s) 184, 248, 407, 695, 735, 955, 992, 1151, 1464
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 2 cut(s) 186, 449
FatI CATG 9 cut(s) 180, 244, 403, 691, 731, 951, 988, 1147, 1460
FauI CCCGC 1 cut(s) 929
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 6 cut(s) 269, 306, 793, 1059, 1267, 1270
FokI GGATG 2 cut(s) 359, 1323
FriOI GRGCYC 2 cut(s) 453, 459
Fsp4HI GCNGC 6 cut(s) 269, 306, 793, 1059, 1267, 1270
FspBI CTAG 3 cut(s) 69, 857, 1440
FspI TGCGCA 2 cut(s) 891, 1040
GlaI GCGC 4 cut(s) 791, 891, 1040, 1273
GluI GCNGC 6 cut(s) 269, 306, 793, 1059, 1267, 1270
GsuI CTGGAG 1 cut(s) 1353
HaeIII GGCC 3 cut(s) 603, 866, 965
HapII CCGG 3 cut(s) 1220, 1382, 1403
HgaI GACGC 1 cut(s) 345
HhaI GCGC 4 cut(s) 792, 892, 1041, 1274
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 9 cut(s) 184, 248, 407, 695, 735, 955, 992, 1151, 1464
Hin6I GCGC 4 cut(s) 790, 890, 1039, 1272
HinP1I GCGC 4 cut(s) 790, 890, 1039, 1272
HincII GTYRAC 1 cut(s) 260
HindII GTYRAC 1 cut(s) 260
HindIII AAGCTT 1 cut(s) 846
HinfI GANTC 6 cut(s) 42, 199, 764, 1052, 1136, 1250
HpaII CCGG 3 cut(s) 1220, 1382, 1403
HphI GGTGA 2 cut(s) 400, 796
Hpy166II GTNNAC 3 cut(s) 186, 260, 1289
Hpy188I TCNGA 7 cut(s) 17, 198, 312, 381, 626, 1163, 1303
Hpy188III TCNNGA 6 cut(s) 236, 594, 647, 662, 1015, 1148
Hpy8I GTNNAC 3 cut(s) 186, 260, 1289
HpyAV CCTTC 6 cut(s) 116, 164, 466, 608, 1405, 1442
HpyCH4III ACNGT 3 cut(s) 257, 551, 644
HpyCH4IV ACGT 2 cut(s) 351, 396
HpyF10VI GCNNNNNNNGC 3 cut(s) 448, 887, 1064
HpyF3I CTNAG 7 cut(s) 48, 197, 311, 447, 1281, 1389, 1407
HpySE526I ACGT 2 cut(s) 351, 396
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 9 cut(s) 184, 248, 407, 695, 735, 955, 992, 1151, 1464
HspAI GCGC 4 cut(s) 790, 890, 1039, 1272
KroI GCCGGC 1 cut(s) 1402
KroNI GCCGGC 1 cut(s) 1404
Kzo9I GATC 3 cut(s) 17, 967, 1032
LguI GCTCTTC 2 cut(s) 17, 645
Lsp1109I GCAGC 5 cut(s) 280, 779, 1045, 1278, 1281
LweI GCATC 6 cut(s) 510, 564, 664, 690, 777, 1283
MaeI CTAG 3 cut(s) 69, 857, 1440
MaeII ACGT 2 cut(s) 351, 396
MaeIII GTNAC 3 cut(s) 325, 406, 662
MalI GATC 3 cut(s) 19, 969, 1034
MboI GATC 3 cut(s) 17, 967, 1032
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 3 cut(s) 453, 459, 1217
MluCI AATT 3 cut(s) 696, 871, 1006
MlyI GAGTC 2 cut(s) 36, 208
MmeI TCCRAC 4 cut(s) 19, 123, 863, 1328
MroNI GCCGGC 1 cut(s) 1402
MseI TTAA 5 cut(s) 423, 606, 1028, 1098, 1343
MslI CAYNNNNRTG 3 cut(s) 299, 660, 932
MspA1I CMGCKG 1 cut(s) 1058
MspI CCGG 3 cut(s) 1220, 1382, 1403
MspR9I CCNGG 2 cut(s) 469, 1382
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 448, 887, 1064
NaeI GCCGGC 1 cut(s) 1404
NciI CCSGG 1 cut(s) 1382
NcoI CCATGG 1 cut(s) 244
NdeII GATC 3 cut(s) 17, 967, 1032
NgoMIV GCCGGC 1 cut(s) 1402
NlaIII CATG 9 cut(s) 184, 248, 407, 695, 735, 955, 992, 1151, 1464
NlaIV GGNNCC 2 cut(s) 465, 477
NmuCI GTSAC 2 cut(s) 406, 662
NsbI TGCGCA 2 cut(s) 891, 1040
NspI RCATGY 2 cut(s) 407, 992
PaeR7I CTCGAG 1 cut(s) 452
PagI TCATGA 1 cut(s) 1147
PceI AGGCCT 1 cut(s) 603
PciSI GCTCTTC 2 cut(s) 17, 645
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 1 cut(s) 922
PdiI GCCGGC 1 cut(s) 1404
PfeI GAWTC 4 cut(s) 764, 1052, 1136, 1250
PkrI GCNGC 6 cut(s) 270, 307, 794, 1060, 1268, 1271
PleI GAGTC 2 cut(s) 36, 207
PpsI GAGTC 2 cut(s) 36, 207
PpuMI RGGWCCY 1 cut(s) 463
Psp124BI GAGCTC 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 465, 477
PspPI GGNCC 2 cut(s) 463, 864
PspPPI RGGWCCY 1 cut(s) 463
PspXI VCTCGAGB 1 cut(s) 452
PsuI RGATCY 1 cut(s) 1032
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 2 cut(s) 759, 1417
RsaNI GTAC 2 cut(s) 758, 1416
RseI CAYNNNNRTG 3 cut(s) 299, 660, 932
SacI GAGCTC 1 cut(s) 453
SapI GCTCTTC 2 cut(s) 17, 645
SaqAI TTAA 5 cut(s) 423, 606, 1028, 1098, 1343
SatI GCNGC 6 cut(s) 269, 306, 793, 1059, 1267, 1270
Sau3AI GATC 3 cut(s) 17, 967, 1032
Sau96I GGNCC 2 cut(s) 463, 864
SchI GAGTC 2 cut(s) 36, 208
ScrFI CCNGG 2 cut(s) 469, 1382
SduI GDGCHC 3 cut(s) 453, 459, 1217
SfaNI GCATC 6 cut(s) 510, 564, 664, 690, 777, 1283
Sfr274I CTCGAG 1 cut(s) 452
SinI GGWCC 1 cut(s) 463
SlaI CTCGAG 1 cut(s) 452
SmiMI CAYNNNNRTG 3 cut(s) 299, 660, 932
SmlI CTYRAG 4 cut(s) 452, 1013, 1292, 1424
SmoI CTYRAG 4 cut(s) 452, 1013, 1292, 1424
Sse9I AATT 3 cut(s) 696, 871, 1006
SseBI AGGCCT 1 cut(s) 603
SsiI CCGC 7 cut(s) 282, 305, 922, 992, 1061, 1167, 1363
SspI AATATT 1 cut(s) 621
SspMI CTAG 3 cut(s) 69, 857, 1440
SstI GAGCTC 1 cut(s) 453
StuI AGGCCT 1 cut(s) 603
StyD4I CCNGG 2 cut(s) 467, 1380
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 3 cut(s) 257, 551, 644
TaiI ACGT 2 cut(s) 354, 399
TaqI TCGA 8 cut(s) 235, 291, 366, 453, 518, 1124, 1197, 1248
TaqII GACCGA 1 cut(s) 971
TasI AATT 3 cut(s) 696, 871, 1006
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 4 cut(s) 764, 1052, 1136, 1250
Tru1I TTAA 5 cut(s) 423, 606, 1028, 1098, 1343
Tru9I TTAA 5 cut(s) 423, 606, 1028, 1098, 1343
TscAI CASTG 8 cut(s) 52, 223, 340, 451, 493, 530, 556, 858
TseFI GTSAC 2 cut(s) 406, 662
TseI GCWGC 5 cut(s) 268, 792, 1058, 1266, 1269
Tsp45I GTSAC 2 cut(s) 406, 662
TspDTI ATGAA 6 cut(s) 135, 695, 929, 1019, 1136, 1257
TspGWI ACGGA 1 cut(s) 1323
TspRI CASTG 8 cut(s) 52, 223, 340, 451, 493, 530, 556, 858
VpaK11BI GGWCC 1 cut(s) 463
XapI RAATTY 1 cut(s) 1006
XceI RCATGY 2 cut(s) 407, 992
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 185
XspI CTAG 3 cut(s) 69, 857, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.