pycom01g11970
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019789
Physical Location & Seq
Forward (+)
12937386 .. 12939815
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11970.15

Sequence Viewer

Length: 1701 bp
ATGAGCGCAAAACAGCATGTAACTGAGGCTAGCACAAGGATTCGCATGAAATTTGAAGAGAGCTTGATTTCAATCTTAGGAGTTGGTGTTGCATGCTCTGCAGACATGCCTGGGGAAAGGTCGGACATCACTGACCAGATAAAATGTTGGATTAGAATAAGCATACTGTTAAGTATTAGAATGTACGTAAAATCTTGTTTCCTTATGTGGAAGTTGAAAACTTCTCTGACAAACTTCAGTCCATTTACTATTCGTACCGTTGAGTTATACTTAGATTATATATTATCAGAATCTGGAACGAGTATCAGATTCGCATGTCAAGATTTCGTAATTTCTATCTTATCGATTTCATTTGTAAAAGGAAAACTTGTATGTTCTAGAGCAAACCCATTTGGTCTTGGTACTCCTGCAAGTCAAAGGATAGGGGAAACCCATAAGGTATTTTGTTCTAGAGCAAACCCATTCGGTCTTGAGTCCCATAAGGTCTTTTTTACAACTAGATACAAAACTAATAGTGTGGGTTTCGTAGATTTTATCCCGAACAGGACGGTGAAAGCTGAAAGAGAGTTTTTATGCCAAAACAAATTCACTTGTATAGACTTAATTGTTACCATGTGTTTGTTTTCGGTTGTGGCGTGGAAGTCTTCGTTCAAGGATTGCACTAAAGACCAATTGGTCTTAGTTGTTGGTGTCAGTTTAATGGGTTGCTGGTGTATGCATTTAACTAGATGCTGTATTTGTGCATTTGGTAAGCATCCGAAGGGGAACACGGAGGAGAGGAAATCAGAGAGAAAAGAGTGGCATCTTGGGGTTGGTGTATCTTGTACTTGTTATTATTCTCATAGTGAAAAGCAATATATTTCTGAGGACATAGGCAGAATTTTGCCGAAATTCGTAAATTTTCCGATGTCTTTATTTCTTGTTTATTTTATTATATTCAACTATGATTTGTATTCTAGTGATGTTATGATAAAAACTAATAATCCTTCAATCGATAGTATCATAGGTCTAGTCAATGCAAAGAAAATGAATACATTTCTATACTATGAATATGGACTCACATATCAAAGAAAGAGTGCACATTATCACTGGTCTAGGTTGACAATATACGTGACAATATACCATGCCCCACTGATATGCGAATTCTATGATCAGATCGATCCATCTTATCTTGACGATTTACCTCTATTTCTCTGCCCCACTGTTTGTTTAATCATGTATAGAGTAAATCACCTCCCTATATTTATGATTTACCTCACAATAGTTCTTACTAAAAAATGTGTCCGCGCTTCGCTGCGGGGATTGAATGTATTAGTCGCAACATGCAAGAATAATTTGCACAGAAAGATAACACACATTAATGCCCTTCACATAAACTGCAGGCCAAGATGCGAAAAAAAACATATAGAACCAACTCTATCCTCAAACACTAAATTTGTTCACTCGAGTCAGAGTCCTCTTGCAAGTATTGTAAAACTCAAAAGCTTACTGTCCAAAACCAGAAACAATTTGTACCATTTACATGCATGTGCAAGATATGTAATCACGGACGGATTTTGTTTTCCTCTGTCCTCCTCAGTCAAAAAAAATTACAAAAACATAGTAGCTTTACTAAAAAAATGCACGATGAAATGGATACATAGTTTTCATGCAGAGAGAGAGAGTACTTTAAATCTTTTTATAGAATATGAGACAATATAA

Protein Analysis

567

Amino Acids

65.07

Weight (kDa)

9.24

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019789)

Species Orthologous Gene IDs
malus_domestica MD11G1202400.v1.1
pyrus_communis pycom01g11970 pycom01g12130 pycom01g12400 pycom01g12410 pycom02g18500 pycom11g01060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 674
AccII CGCG 1 cut(s) 1287
AciI CCGC 2 cut(s) 1285, 1297
AclWI GGATC 1 cut(s) 1154
AcsI RAATTY 7 cut(s) 50, 584, 879, 890, 898, 1142, 1433
AcuI CTGAAG 1 cut(s) 220
AfaI GTAC 6 cut(s) 185, 256, 403, 826, 1514, 1666
AgsI TTSAA 7 cut(s) 56, 72, 217, 652, 940, 990, 1306
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 4 cut(s) 63, 557, 1485, 1607
AluI AGCT 4 cut(s) 63, 557, 1485, 1607
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 1685
Alw44I GTGCAC 1 cut(s) 1077
AlwI GGATC 1 cut(s) 1154
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 1444
AoxI GGCC 1 cut(s) 1382
ApaLI GTGCAC 1 cut(s) 1077
ApeKI GCWGC 1 cut(s) 1294
ApoI RAATTY 7 cut(s) 50, 584, 879, 890, 898, 1142, 1433
AseI ATTAAT 1 cut(s) 1359
AspLEI GCGC 2 cut(s) 8, 1289
AsuHPI GGTGA 2 cut(s) 562, 1223
AsuNHI GCTAGC 1 cut(s) 29
AvaI CYCGRG 1 cut(s) 1444
BaeGI GKGCMC 1 cut(s) 1081
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 1 cut(s) 1281
BccI CCATC 1 cut(s) 1171
BciT130I CCWGG 1 cut(s) 111
BciVI GTATCC 1 cut(s) 1629
BclI TGATCA 1 cut(s) 1150
BcoDI GTCTC 1 cut(s) 1685
BfaI CTAG 8 cut(s) 30, 378, 450, 498, 726, 957, 1010, 1095
BfmI CTRYAG 2 cut(s) 99, 1378
BfuI GTATCC 1 cut(s) 1629
BisI GCNGC 1 cut(s) 1295
BlsI GCNGC 1 cut(s) 1296
BmcAI AGTACT 1 cut(s) 1666
Bme1390I CCNGG 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 1444
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 4 cut(s) 719, 763, 811, 1379
BmtI GCTAGC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 491
Bsa29I ATCGAT 3 cut(s) 344, 993, 1158
BsaAI YACGTR 2 cut(s) 187, 1111
BsaBI GATNNNNATC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bse1I ACTGG 1 cut(s) 1094
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 1 cut(s) 111
BseCI ATCGAT 3 cut(s) 344, 993, 1158
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 1 cut(s) 754
BseJI GATNNNNATC 1 cut(s) 71
BseMII CTCAG 3 cut(s) 15, 855, 1590
BseNI ACTGG 1 cut(s) 1094
BseRI GAGGAG 2 cut(s) 788, 1564
BseSI GKGCMC 1 cut(s) 1081
BseXI GCAGC 1 cut(s) 1281
Bsh1236I CGCG 1 cut(s) 1287
BshFI GGCC 1 cut(s) 1384
BshVI ATCGAT 3 cut(s) 344, 993, 1158
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 1 cut(s) 1444
BslFI GGGAC 1 cut(s) 460
BsmAI GTCTC 1 cut(s) 1685
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 1 cut(s) 1384
BsoBI CYCGRG 1 cut(s) 1444
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp143I GATC 3 cut(s) 1150, 1155, 1159
BspACI CCGC 2 cut(s) 1285, 1297
BspANI GGCC 1 cut(s) 1384
BspCNI CTCAG 3 cut(s) 16, 856, 1589
BspDI ATCGAT 3 cut(s) 344, 993, 1158
BspFNI CGCG 1 cut(s) 1287
BspMAI CTGCAG 2 cut(s) 103, 1382
BspOI GCTAGC 1 cut(s) 33
BspPI GGATC 1 cut(s) 1154
BsrI ACTGG 1 cut(s) 1094
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 3 cut(s) 1150, 1155, 1159
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 5 cut(s) 168, 259, 550, 1204, 1491
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 2 cut(s) 187, 1111
BstC8I GCNNGC 3 cut(s) 31, 94, 1382
BstDEI CTNAG 6 cut(s) 24, 76, 271, 679, 864, 1576
BstF5I GGATG 1 cut(s) 754
BstFNI CGCG 1 cut(s) 1287
BstHHI GCGC 2 cut(s) 8, 1289
BstKTI GATC 3 cut(s) 1153, 1158, 1162
BstMAI GTCTC 1 cut(s) 1685
BstMBI GATC 3 cut(s) 1150, 1155, 1159
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 7 cut(s) 20, 96, 109, 318, 1326, 1526, 1530
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 2 cut(s) 99, 1378
BstSLI GKGCMC 1 cut(s) 1081
BstSNI TACGTA 1 cut(s) 187
BstUI CGCG 1 cut(s) 1287
BstV1I GCAGC 1 cut(s) 1281
BstV2I GAAGAC 1 cut(s) 636
Bsu15I ATCGAT 3 cut(s) 344, 993, 1158
BsuI GTATCC 1 cut(s) 1629
BsuRI GGCC 1 cut(s) 1384
BsuTUI ATCGAT 3 cut(s) 344, 993, 1158
BtsCI GGATG 1 cut(s) 754
BtsIMutI CAGTG 4 cut(s) 129, 1087, 1130, 1200
Cac8I GCNNGC 3 cut(s) 31, 94, 1382
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 2 cut(s) 8, 1289
ClaI ATCGAT 3 cut(s) 344, 993, 1158
Csp6I GTAC 6 cut(s) 184, 255, 402, 825, 1513, 1665
CviJI RGCY 6 cut(s) 29, 63, 557, 1384, 1485, 1607
CviKI_1 RGCY 6 cut(s) 29, 63, 557, 1384, 1485, 1607
CviQI GTAC 6 cut(s) 184, 255, 402, 825, 1513, 1665
DdeI CTNAG 6 cut(s) 24, 76, 271, 679, 864, 1576
DpnI GATC 3 cut(s) 1152, 1157, 1161
DpnII GATC 3 cut(s) 1150, 1155, 1159
DraI TTTAAA 1 cut(s) 1671
DrdI GACNNNNNNGTC 1 cut(s) 674
DseDI GACNNNNNNGTC 1 cut(s) 674
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco105I TACGTA 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 1444
EcoRI GAATTC 1 cut(s) 1142
EcoRII CCWGG 1 cut(s) 109
EcoT22I ATGCAT 2 cut(s) 720, 1528
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 1 cut(s) 460
FauI CCCGC 1 cut(s) 1290
FbaI TGATCA 1 cut(s) 1150
Fnu4HI GCNGC 1 cut(s) 1295
FokI GGATG 1 cut(s) 741
Fsp4HI GCNGC 1 cut(s) 1295
FspBI CTAG 8 cut(s) 30, 378, 450, 498, 726, 957, 1010, 1095
GlaI GCGC 2 cut(s) 7, 1288
GluI GCNGC 1 cut(s) 1295
HaeIII GGCC 1 cut(s) 1384
HhaI GCGC 2 cut(s) 8, 1289
Hin6I GCGC 2 cut(s) 6, 1287
HinP1I GCGC 2 cut(s) 6, 1287
HincII GTYRAC 1 cut(s) 1101
HindII GTYRAC 1 cut(s) 1101
HindIII AAGCTT 1 cut(s) 1483
HinfI GANTC 7 cut(s) 40, 290, 309, 473, 1056, 1447, 1453
HphI GGTGA 2 cut(s) 562, 1223
Hpy166II GTNNAC 3 cut(s) 1079, 1101, 1441
Hpy188III TCNNGA 7 cut(s) 294, 320, 378, 450, 470, 538, 1172
Hpy8I GTNNAC 3 cut(s) 1079, 1101, 1441
HpyAV CCTTC 3 cut(s) 754, 996, 1376
HpyCH4III ACNGT 5 cut(s) 168, 259, 550, 1204, 1491
HpyCH4IV ACGT 2 cut(s) 186, 1110
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 6 cut(s) 24, 76, 271, 679, 864, 1576
HpySE526I ACGT 2 cut(s) 186, 1110
HspAI GCGC 2 cut(s) 6, 1287
Ksp22I TGATCA 1 cut(s) 1150
Kzo9I GATC 3 cut(s) 1150, 1155, 1159
Lsp1109I GCAGC 1 cut(s) 1281
LweI GCATC 4 cut(s) 719, 763, 811, 1379
MaeI CTAG 8 cut(s) 30, 378, 450, 498, 726, 957, 1010, 1095
MaeII ACGT 2 cut(s) 186, 1110
MaeIII GTNAC 3 cut(s) 19, 607, 1111
MalI GATC 3 cut(s) 1152, 1157, 1161
MboI GATC 3 cut(s) 1150, 1155, 1159
MboII GAAGA 2 cut(s) 68, 636
MfeI CAATTG 1 cut(s) 671
MhlI GDGCHC 1 cut(s) 1081
MlyI GAGTC 4 cut(s) 482, 1050, 1456, 1462
MmeI TCCRAC 2 cut(s) 102, 128
Mph1103I ATGCAT 2 cut(s) 720, 1528
MseI TTAA 7 cut(s) 170, 602, 698, 722, 1211, 1359, 1670
MslI CAYNNNNRTG 4 cut(s) 1135, 1359, 1521, 1527
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 1 cut(s) 671
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 1287
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 3 cut(s) 1150, 1155, 1159
NheI GCTAGC 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 1111
NsiI ATGCAT 2 cut(s) 720, 1528
NspI RCATGY 7 cut(s) 20, 96, 109, 318, 1326, 1526, 1530
PaeI GCATGC 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 1444
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PfeI GAWTC 3 cut(s) 40, 290, 309
PkrI GCNGC 1 cut(s) 1296
PleI GAGTC 4 cut(s) 481, 1050, 1455, 1461
PpsI GAGTC 4 cut(s) 481, 1050, 1455, 1461
Ppu21I YACGTR 2 cut(s) 187, 1111
PshBI ATTAAT 1 cut(s) 1359
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 1444
PstI CTGCAG 2 cut(s) 103, 1382
PstNI CAGNNNCTG 1 cut(s) 293
RsaI GTAC 6 cut(s) 185, 256, 403, 826, 1514, 1666
RsaNI GTAC 6 cut(s) 184, 255, 402, 825, 1513, 1665
RseI CAYNNNNRTG 4 cut(s) 1135, 1359, 1521, 1527
SaqAI TTAA 7 cut(s) 170, 602, 698, 722, 1211, 1359, 1670
SatI GCNGC 1 cut(s) 1295
Sau3AI GATC 3 cut(s) 1150, 1155, 1159
ScaI AGTACT 1 cut(s) 1666
SchI GAGTC 4 cut(s) 482, 1050, 1456, 1462
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 4 cut(s) 719, 763, 811, 1379
SfcI CTRYAG 2 cut(s) 99, 1378
Sfr274I CTCGAG 1 cut(s) 1444
SlaI CTCGAG 1 cut(s) 1444
SmiMI CAYNNNNRTG 4 cut(s) 1135, 1359, 1521, 1527
SmlI CTYRAG 2 cut(s) 470, 1444
SmoI CTYRAG 2 cut(s) 470, 1444
SnaBI TACGTA 1 cut(s) 187
SphI GCATGC 1 cut(s) 96
SsiI CCGC 2 cut(s) 1285, 1297
SspMI CTAG 8 cut(s) 30, 378, 450, 498, 726, 957, 1010, 1095
StyD4I CCNGG 1 cut(s) 109
TaaI ACNGT 5 cut(s) 168, 259, 550, 1204, 1491
TaiI ACGT 2 cut(s) 189, 1113
TaqI TCGA 4 cut(s) 344, 993, 1158, 1445
TaqII GACCGA 1 cut(s) 455
TatI WGTACW 2 cut(s) 824, 1664
TfiI GAWTC 3 cut(s) 40, 290, 309
Tru1I TTAA 7 cut(s) 170, 602, 698, 722, 1211, 1359, 1670
Tru9I TTAA 7 cut(s) 170, 602, 698, 722, 1211, 1359, 1670
TscAI CASTG 4 cut(s) 136, 1094, 1137, 1207
TseFI GTSAC 1 cut(s) 1111
TseI GCWGC 1 cut(s) 1294
Tsp45I GTSAC 1 cut(s) 1111
TspDTI ATGAA 6 cut(s) 62, 339, 1043, 1062, 1637, 1643
TspGWI ACGGA 3 cut(s) 785, 1562, 1566
TspRI CASTG 4 cut(s) 136, 1094, 1137, 1207
VneI GTGCAC 1 cut(s) 1077
VspI ATTAAT 1 cut(s) 1359
XapI RAATTY 7 cut(s) 50, 584, 879, 890, 898, 1142, 1433
XbaI TCTAGA 2 cut(s) 377, 449
XceI RCATGY 7 cut(s) 20, 96, 109, 318, 1326, 1526, 1530
XhoI CTCGAG 1 cut(s) 1444
XspI CTAG 8 cut(s) 30, 378, 450, 498, 726, 957, 1010, 1095
ZrmI AGTACT 1 cut(s) 1666
Zsp2I ATGCAT 2 cut(s) 720, 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.