pycom02g18500

Ribulose bisphosphate carboxylase oxygenase activase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0019789
Physical Location & Seq
Forward (+)
16670483 .. 16670981
499 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18500.3

Sequence Viewer

Length: 402 bp
ATGAAACAAAGGAGTGAGTCCTACACCACAAGGACTCGAAGATGCCGATGCGAAAGTGCCTGGACGTCGGGATTGTATGCCTCGATTCTATGTCCTGAAGGGGGCAAAATAGTTATTAACGTACTAACCCCCAGGTTTTATGAAAACACAAATATAATGATAAACATAGGTTTTCCAAAACCTAGCATGTCGTGCAATGTCTCAAATCATAATTCGCCCCATGCCAGCTCCCCGTCTCTGAGCAGACCGTCAGAGGAAAATACACTTCAGGCCCCCTGCCGGCTCCCCATCTTTGAGCAGACAGTTAGAGGAAAATACACTTCAGGCCCTATGCCAACACCAAACCGTCGCCCGGGACGGACCGGATCGATCCCTACGTTCCGTAGCGGAAAGGACCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.83

Weight (kDa)

9.97

Isoelectric Point (pI)

75.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019789)

Species Orthologous Gene IDs
malus_domestica MD11G1202400.v1.1
pyrus_communis pycom01g11970 pycom01g12130 pycom01g12400 pycom01g12410 pycom02g18500 pycom11g01060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 68
AciI CCGC 1 cut(s) 387
AclWI GGATC 2 cut(s) 364, 373
AcuI CTGAAG 3 cut(s) 117, 251, 306
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 101, 279, 352
AjnI CCWGG 2 cut(s) 59, 131
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 205, 240
AlwI GGATC 2 cut(s) 364, 373
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 2 cut(s) 270, 325
AspS9I GGNCC 4 cut(s) 271, 326, 360, 394
AsuC2I CCSGG 2 cut(s) 353, 354
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 2 cut(s) 360, 394
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 2 cut(s) 61, 133
BcnI CCSGG 2 cut(s) 353, 354
BcoDI GTCTC 2 cut(s) 205, 240
BfaI CTAG 2 cut(s) 183, 400
Bme1390I CCNGG 4 cut(s) 61, 133, 353, 354
Bme18I GGWCC 2 cut(s) 360, 394
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 4 cut(s) 271, 326, 360, 394
BmiI GGNNCC 2 cut(s) 273, 284
BmrFI CCNGG 4 cut(s) 61, 133, 353, 354
BmsI GCATC 2 cut(s) 32, 38
BpuMI CCSGG 2 cut(s) 353, 354
Bsa29I ATCGAT 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 131, 352
BsaWI WCCGGW 1 cut(s) 362
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 279, 352
Bse118I RCCGGY 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 202
BseBI CCWGG 2 cut(s) 61, 133
BseCI ATCGAT 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 131, 352
BseLI CCNNNNNNNGG 3 cut(s) 101, 279, 352
BseMI GCAATG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 230
BshFI GGCC 2 cut(s) 272, 327
BshVI ATCGAT 1 cut(s) 368
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 3 cut(s) 280, 353, 363
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 101, 279, 352
BsmAI GTCTC 2 cut(s) 205, 240
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 2 cut(s) 272, 327
BsoBI CYCGRG 1 cut(s) 352
Bsp143I GATC 2 cut(s) 365, 369
BspACI CCGC 1 cut(s) 387
BspANI GGCC 2 cut(s) 272, 327
BspCNI CTCAG 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 273, 284
BspPI GGATC 2 cut(s) 364, 373
BsrDI GCAATG 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 279
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 131, 352
BssMI GATC 2 cut(s) 365, 369
BssNI GRCGYC 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 61, 133
Bst4CI ACNGT 3 cut(s) 249, 304, 347
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 226, 281
BstDEI CTNAG 1 cut(s) 239
BstKTI GATC 2 cut(s) 368, 372
BstMAI GTCTC 2 cut(s) 205, 240
BstMBI GATC 2 cut(s) 365, 369
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 2 cut(s) 61, 133
BstNSI RCATGY 1 cut(s) 190
BstSCI CCNGG 4 cut(s) 59, 131, 351, 352
Bsu15I ATCGAT 1 cut(s) 368
BsuRI GGCC 2 cut(s) 272, 327
BsuTUI ATCGAT 1 cut(s) 368
Cac8I GCNNGC 2 cut(s) 226, 281
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 4 cut(s) 271, 326, 360, 394
Cfr9I CCCGGG 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 368
CpoI CGGWCCG 1 cut(s) 360
Csp6I GTAC 1 cut(s) 122
CspI CGGWCCG 1 cut(s) 360
CviAII CATG 2 cut(s) 187, 221
CviJI RGCY 4 cut(s) 228, 272, 283, 327
CviKI_1 RGCY 4 cut(s) 228, 272, 283, 327
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 1 cut(s) 239
DpnI GATC 2 cut(s) 367, 371
DpnII GATC 2 cut(s) 365, 369
Eco47I GGWCC 2 cut(s) 360, 394
Eco57I CTGAAG 3 cut(s) 117, 251, 306
Eco88I CYCGRG 1 cut(s) 352
EcoO109I RGGNCCY 2 cut(s) 271, 326
EcoRII CCWGG 2 cut(s) 59, 131
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 2 cut(s) 190, 224
FaiI YATR 9 cut(s) 78, 91, 141, 155, 167, 188, 210, 222, 332
FaqI GGGAC 1 cut(s) 369
FatI CATG 2 cut(s) 186, 220
FspBI CTAG 2 cut(s) 183, 400
HaeIII GGCC 2 cut(s) 272, 327
HapII CCGG 3 cut(s) 280, 353, 363
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 2 cut(s) 190, 224
HinfI GANTC 3 cut(s) 17, 34, 85
HpaII CCGG 3 cut(s) 280, 353, 363
Hpy188I TCNGA 2 cut(s) 240, 253
Hpy188III TCNNGA 2 cut(s) 69, 95
Hpy99I CGWCG 2 cut(s) 70, 351
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 3 cut(s) 249, 304, 347
HpyCH4IV ACGT 3 cut(s) 65, 120, 377
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 3 cut(s) 65, 120, 377
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 2 cut(s) 190, 224
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Kzo9I GATC 2 cut(s) 365, 369
LmnI GCTCC 2 cut(s) 233, 288
LweI GCATC 2 cut(s) 32, 38
MaeI CTAG 2 cut(s) 183, 400
MaeII ACGT 3 cut(s) 65, 120, 377
MalI GATC 2 cut(s) 367, 371
MboI GATC 2 cut(s) 365, 369
MboII GAAGA 1 cut(s) 51
MluCI AATT 1 cut(s) 211
MlyI GAGTC 2 cut(s) 26, 28
MnlI CCTC 3 cut(s) 91, 247, 302
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 1 cut(s) 117
MspI CCGG 3 cut(s) 280, 353, 363
MspR9I CCNGG 4 cut(s) 61, 133, 353, 354
MvaI CCWGG 2 cut(s) 61, 133
MwoI GCNNNNNNNGC 1 cut(s) 192
NaeI GCCGGC 1 cut(s) 281
NciI CCSGG 2 cut(s) 353, 354
NdeII GATC 2 cut(s) 365, 369
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 2 cut(s) 190, 224
NlaIV GGNNCC 2 cut(s) 273, 284
NspI RCATGY 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 43, 374
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 85
PleI GAGTC 2 cut(s) 25, 28
PpsI GAGTC 2 cut(s) 25, 28
Psp6I CCWGG 2 cut(s) 59, 131
PspGI CCWGG 2 cut(s) 59, 131
PspN4I GGNNCC 2 cut(s) 273, 284
PspPI GGNCC 4 cut(s) 271, 326, 360, 394
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
Rsr2I CGGWCCG 1 cut(s) 360
RsrII CGGWCCG 1 cut(s) 360
SaqAI TTAA 1 cut(s) 117
Sau3AI GATC 2 cut(s) 365, 369
Sau96I GGNCC 4 cut(s) 271, 326, 360, 394
SchI GAGTC 2 cut(s) 26, 28
ScrFI CCNGG 4 cut(s) 61, 133, 353, 354
SetI ASST 7 cut(s) 68, 123, 137, 172, 184, 230, 380
SfaNI GCATC 2 cut(s) 32, 38
SinI GGWCC 2 cut(s) 360, 394
SmaI CCCGGG 1 cut(s) 354
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 387
SspMI CTAG 2 cut(s) 183, 400
StyD4I CCNGG 4 cut(s) 59, 131, 351, 352
TaaI ACNGT 3 cut(s) 249, 304, 347
TaiI ACGT 3 cut(s) 68, 123, 380
TaqI TCGA 3 cut(s) 37, 83, 368
TasI AATT 1 cut(s) 211
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TspDTI ATGAA 2 cut(s) 17, 156
TspGWI ACGGA 2 cut(s) 371, 373
TspMI CCCGGG 1 cut(s) 352
VpaK11BI GGWCC 2 cut(s) 360, 394
XceI RCATGY 1 cut(s) 190
XmaI CCCGGG 1 cut(s) 352
XspI CTAG 2 cut(s) 183, 400
ZraI GACGTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.