pycom01g14010

Low temperature-induced protein lt101.2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0018717
Physical Location & Seq
Forward (+)
14309328 .. 14310225
898 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14010.1

Sequence Viewer

Length: 249 bp
ATGTTGCACAAAGTTTATCCACGCACAAAATCGTCACCCATATTTAGCACAAGTTGTGGTTTTCATTTTTCAATCGTCAGAAAAATGGGTTCAGAAACGTTTCTGGAAGTGATATTGGCAATCATCCTCCCACCAGTGGGAGTCTTCCTTCGTTATGGCTGTGCAGTGGAGTTCTGGATATGTTTGTTGCTGACAATATTGGGATACATACCTGGAATCATATACGCATTATATGTACTCGTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pmp3

Protein Analysis

83

Amino Acids

9.18

Weight (kDa)

8.93

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pmp3 PF01679 34 - 80 1.3e-21 Proteolipid membrane potential modulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018717)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38905
malus_domestica MD01G1131300.v1.1 MD07G1179400.v1.1
prunus_persica Prupe.2G218700_v2.0.a1
pyrus_communis pycom01g14010
rosa_chinensis RchiOBHm_Chr1g0364141
rosa_roxburghii Rroxscaffold_4G00292600
rosa_rugosa Rorug01G0313900
rosa_wichuraiana Rw1G028500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 98
AfaI GTAC 1 cut(s) 237
AfiI CCNNNNNNNGG 2 cut(s) 136, 137
AgsI TTSAA 1 cut(s) 72
AjnI CCWGG 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 213
BciVI GTATCC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 136
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 137
Bse1I ACTGG 1 cut(s) 134
BseBI CCWGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 136, 137
BseNI ACTGG 1 cut(s) 134
BsgI GTGCAG 1 cut(s) 183
BslI CCNNNNNNNGG 2 cut(s) 136, 137
BsrI ACTGG 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 123
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 136
BsuI GTATCC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 123
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 141, 171
Csp6I GTAC 1 cut(s) 236
CviJI RGCY 1 cut(s) 159
CviKI_1 RGCY 1 cut(s) 159
CviQI GTAC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 211
FaiI YATR 8 cut(s) 41, 156, 181, 209, 221, 223, 232, 234
FokI GGATG 1 cut(s) 110
HinfI GANTC 2 cut(s) 141, 216
HphI GGTGA 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 80, 94, 245
Hpy188III TCNNGA 2 cut(s) 104, 175
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 2 cut(s) 7, 164
HpySE526I ACGT 1 cut(s) 98
LpnPI CCDG 5 cut(s) 89, 147, 160, 198, 225
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 33
MboII GAAGA 1 cut(s) 136
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 99
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 216
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp1406I AACGTT 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 213
SetI ASST 2 cut(s) 101, 214
SgeI CNNG 7 cut(s) 33, 63, 116, 146, 187, 224, 225
SspI AATATT 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 211
TaiI ACGT 1 cut(s) 101
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 1 cut(s) 216
TscAI CASTG 2 cut(s) 141, 171
TseFI GTSAC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 1 cut(s) 53
TspRI CASTG 2 cut(s) 141, 171
XmnI GAANNNNTTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.