Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0009798

Physical Location & Seq

Genomic Coordinates Forward (+)

16450655 .. 16454384

Gene Structure 3730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGACGATAAAATGTCAGATTGTACAGCACGGTTATTGGTGTGGTGGGTCCCACCCCACCACCCCATCATATTCCGTTCCTTTCCTTCTGGAGTTACTTCACTCTCCCACCCATCTCTCAAATTCCCCAAGCAACCGGACACCGACGCGGGCGAATCTCCTCCAAATCCTCCGGCTCCGAATTCCGATCCGAACAAGGCGATTCTCCATGTCGAAGCAGACCTACAGAGTGTGCTTCTGCTTCCAGCGGCGCTTCAAGCTCGCCGTCAAGGAAGCACCGCCGGAGATCAAGGCCTTGTTCGACCAGTACTCGGAGAACGGCATCATGACCGCCGATCACTTCCACCGATTCCTGACGGAAGTCCAGAAGGAGGAGAAAGCCACCAAGGAGGAAGCTCAGGCCCTCATCGAACAGAGCCTCTCCGAGCTCAAACACCTCAACATCTTCCACCGCAAGGTCCTCGATCTCGAAGCATTCTTCAAGTACCTCTTTGGCGACATCAACCCCCCAAACTCTCTATCTCTCGGGGTGCACCATGATATGAATGCGCCATTGTCGCATTATTTCATATACACAGGGCACAATTCGTATCTGACTGGGAACCAGCTGAGCAGTGACTGCAGTGACGTTCCTATTATTCAAGCGCTTCACAGAGGAGTGAGGGTGATTGAGCTGGATATTTGGCCGAATTCGAATAAAGACGATGTCGATGTGCTTCATGGAGGGACATTGACTACACCGGTGGAACTTATCAAGTGTTTGAAGTCTATTAAGGAACATGCCTTTGTTGCCTCTGAATACCCTGTTGTAATCACTTTGGAAGACCACCTGACCCCGGATCTTCAAACTAAAGTCGCTAAGATGGTCACTGAAACATTTGGAGAGGTACTGTTTTCTCCTGGCCCGGAATGTCTGAAAGAATTTCCATCGCCAGCGTCACTAAAGAAAAGAATTATTATCTCGACCAAACCGCCTCAGGAATATCGTGAGGCAAAGGATGCTCTGCAAAGTGAAAGTGATCCAAAGAGTGGAAAAACTCCAGCTGATGAGGAAGCGTGGGGTAAAGAAGTCCCGGACGCCAAAGTTGCAATTGCAGTAGATGACAAGCACAATGAATTGGATGAAGAAGATAGCAATGAGGAGGAAGATGTTGATAATGGTGGTTCCAAGTCTGTTGCACCTGAATATAAGCGTTTAATTGCCATTCATGCTGGGAAACCCAAAGGTGGATTAGTGGAGTGTCTCAAAGTAGATCCCAATAAAGTGCGGCGTCTTAGCTTAAGTGAGCAACAGCTCGAGAAGGCAGTAGTAACCTATGGAAAGGAAATTGTCAGGTTTACTCAGAGGAATATTTTGAGGGTGTACCCAAAGGGCATACGTGTTGACTCATCAAATTATAACCCAATGATTGGGTGGTCGCATGGGGCTCAAATGGTTGCATTCAATATGCAGGGATATGGAAGATCATTGTGGTTGATGCAGGGAATGTTTAGAGCCAATGGTGGGTGCGGATATGTGAAAAAACCGGATTTTCTTTTGAAGAGTGGTCCACATAATGAGGTCTGGCATGCCATGATTGATCCTAAATATGATTTACCAGTGAAACAAACTTTGAAGGTAAAAGTATATATGGGAGAAGGATGGTACTATGATTTCAAGCATACACATTTCGATGCATATTCTCCTCCAGATTTTTATGCAAGGGTAGGAATTGCTGGAGTTGCTGCTGATAGTGTGATGAAGAAGACGAAGACCCTAGAGGACGACTGGATTCCAGCGTGGAACGAAGAATTTGAATTCCCATTAACTGTTCCAGAACTAGCCGTGCTCCGCATTGAAGTTCACGAGTATGACATGTCAGAAAAGGATGATTTTGGAGGCCAAACATGCCTACCGGTGTCTGAGCTCAGAAGAGGGATTCGAGCAGTTCCCTTACACAGCCAGAAGGGAGAGAAGTACAAATCCGTAAAGCTTCTCATGCACTTCGATTTTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007049 GO:0007275 GO:0008081 GO:0008150 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0034293 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0061458 GO:0065007 GO:0065008 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0098542 GO:0099402 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

666

Amino Acids

75.53

Weight (kDa)

6.53

Isoelectric Point (pI)

47.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_like	PF09279	95 - 163	2.8e-06	Phosphoinositide-specific phospholipase C, efhand-like
PI-PLC-X	PF00388	181 - 323	1.4e-52	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	425 - 511	1.3e-29	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	537 - 638	1.1e-12	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009798)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08510 AT3G08510 AT3G08510 AT3G08510 AT3G55940
fragaria_vesca	FvH4_7g24471 FvH4_7g24471
malus_domestica	MD01G1153000.v1.1 MD07G1221900.v1.1
prunus_persica	Prupe.2G250100_v2.0.a1
pyrus_communis	pycom01g17060 pycom07g20040
rosa_chinensis	RchiOBHm_Chr1g0370231
rosa_laevigata	RLG00000027067
rosa_multiflora	Rmu_sc0017405.1_g000001 Rmu_sc0030456.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286730
rosa_rugosa	Rorug01G0354700 Rorug01G0354800
rosa_samantha	Rh1AG363500
rosa_wichuraiana	Rw1G031990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1398
AccB7I	CCANNNNNTGG	2 cut(s)	1028, 1409
AccII	CGCG	1 cut(s)	148
AciI	CCGC	9 cut(s)	148, 247, 278, 330, 451, 971, 1267, 1509, 1831
AclWI	GGATC	5 cut(s)	181, 846, 1013, 1247, 1574
AcoI	YGGCCR	1 cut(s)	683
AcsI	RAATTY	6 cut(s)	121, 180, 688, 920, 1790, 1796
AcyI	GRCGYC	2 cut(s)	1077, 1270
AfaI	GTAC	7 cut(s)	24, 308, 485, 888, 1364, 1646, 1958
AfeI	AGCGCT	1 cut(s)	645
AfiI	CCNNNNNNNGG	8 cut(s)	310, 370, 454, 835, 1028, 1226, 1409, 1503
AflII	CTTAAG	1 cut(s)	1279
AflIII	ACRYGT	2 cut(s)	1378, 1854
AgeI	ACCGGT	2 cut(s)	739, 1894
AjnI	CCWGG	1 cut(s)	898
Alw21I	GWGCWC	4 cut(s)	429, 534, 1830, 1908
Alw26I	GTCTC	1 cut(s)	1247
Alw44I	GTGCAC	1 cut(s)	530
AlwI	GGATC	5 cut(s)	181, 846, 1013, 1247, 1574
AlwNI	CAGNNNCTG	1 cut(s)	618
Ama87I	CYCGRG	2 cut(s)	524, 1295
Aor51HI	AGCGCT	1 cut(s)	645
AoxI	GGCC	5 cut(s)	291, 399, 683, 901, 1879
ApaLI	GTGCAC	1 cut(s)	530
ApeKI	GCWGC	1 cut(s)	1724
ApoI	RAATTY	6 cut(s)	121, 180, 688, 920, 1790, 1796
AsiGI	ACCGGT	2 cut(s)	739, 1894
AspLEI	GCGC	3 cut(s)	252, 550, 646
AspS9I	GGNCC	5 cut(s)	48, 400, 457, 902, 1547
AsuC2I	CCSGG	3 cut(s)	836, 905, 1073
AsuHPI	GGTGA	1 cut(s)	676
AsuII	TTCGAA	1 cut(s)	692
AvaI	CYCGRG	2 cut(s)	524, 1295
AvaII	GGWCC	3 cut(s)	48, 457, 1547
AxyI	CCTNAGG	1 cut(s)	975
BaeGI	GKGCMC	2 cut(s)	534, 582
BanII	GRGCYC	3 cut(s)	429, 1429, 1908
BarI	GAAGNNNNNNTAC	2 cut(s)	206, 238
BauI	CACGAG	1 cut(s)	1844
BbsI	GAAGAC	3 cut(s)	828, 1751, 1757
Bbv12I	GWGCWC	4 cut(s)	429, 534, 1830, 1908
BbvI	GCAGC	1 cut(s)	1711
BccI	CCATC	5 cut(s)	73, 120, 856, 934, 1635
BceAI	ACGGC	3 cut(s)	248, 334, 1808
BcgI	CGANNNNNNTGC	2 cut(s)	442, 476
BciT130I	CCWGG	1 cut(s)	900
BcnI	CCSGG	3 cut(s)	836, 905, 1073
BcoDI	GTCTC	1 cut(s)	1247
BfaI	CTAG	2 cut(s)	1757, 1820
BfmI	CTRYAG	2 cut(s)	223, 619
BfoI	RGCGCY	2 cut(s)	253, 647
BfrI	CTTAAG	1 cut(s)	1279
BisI	GCNGC	3 cut(s)	248, 1268, 1725
BlpI	GCTNAGC	1 cut(s)	608
BlsI	GCNGC	3 cut(s)	249, 1269, 1726
BmcAI	AGTACT	1 cut(s)	308
Bme1390I	CCNGG	4 cut(s)	836, 900, 905, 1073
Bme18I	GGWCC	3 cut(s)	48, 457, 1547
BmeT110I	CYCGRG	2 cut(s)	524, 1295
BmgT120I	GGNCC	5 cut(s)	48, 400, 457, 902, 1547
BmiI	GGNNCC	5 cut(s)	49, 50, 176, 602, 1165
BmrFI	CCNGG	4 cut(s)	836, 900, 905, 1073
BmrI	ACTGGG	1 cut(s)	606
BmsI	GCATC	4 cut(s)	330, 988, 1467, 1663
BmuI	ACTGGG	1 cut(s)	606
BoxI	GACNNNNGTC	1 cut(s)	359
BpiI	GAAGAC	3 cut(s)	828, 1751, 1757
BpmI	CTGGAG	4 cut(s)	110, 1023, 1671, 1737
Bpu10I	CCTNAGC	1 cut(s)	396
Bpu1102I	GCTNAGC	1 cut(s)	608
Bpu14I	TTCGAA	1 cut(s)	692
BpuMI	CCSGG	3 cut(s)	836, 905, 1073
BsaAI	YACGTR	1 cut(s)	1379
BsaHI	GRCGYC	2 cut(s)	1077, 1270
BsaJI	CCNNGG	2 cut(s)	384, 834
BsaWI	WCCGGW	4 cut(s)	135, 739, 1525, 1894
BsaXI	ACNNNNNCTCC	2 cut(s)	648, 678
Bsc4I	CCNNNNNNNGG	8 cut(s)	310, 370, 454, 835, 1028, 1226, 1409, 1503
Bse118I	RCCGGY	2 cut(s)	739, 1894
Bse1I	ACTGG	4 cut(s)	304, 601, 1598, 1772
Bse21I	CCTNAGG	1 cut(s)	975
Bse3DI	GCAATG	1 cut(s)	1141
BseBI	CCWGG	1 cut(s)	900
BseDI	CCNNGG	2 cut(s)	384, 834
BseGI	GGATG	4 cut(s)	1003, 1126, 1646, 1873
BseLI	CCNNNNNNNGG	8 cut(s)	310, 370, 454, 835, 1028, 1226, 1409, 1503
BseMI	GCAATG	1 cut(s)	1141
BseMII	CTCAG	6 cut(s)	410, 599, 989, 1355, 1893, 1921
BseNI	ACTGG	4 cut(s)	304, 601, 1598, 1772
BseRI	GAGGAG	5 cut(s)	149, 386, 669, 1154, 1674
BseSI	GKGCMC	2 cut(s)	534, 582
BseXI	GCAGC	1 cut(s)	1711
BseYI	CCCAGC	1 cut(s)	1211
Bsh1236I	CGCG	1 cut(s)	148
BshFI	GGCC	5 cut(s)	293, 401, 685, 903, 1881
BshTI	ACCGGT	2 cut(s)	739, 1894
BsiHKAI	GWGCWC	4 cut(s)	429, 534, 1830, 1908
BsiHKCI	CYCGRG	2 cut(s)	524, 1295
BsiSI	CCGG	9 cut(s)	136, 172, 281, 740, 836, 905, 1073, 1526, 1895
BslFI	GGGAC	3 cut(s)	34, 739, 1055
BslI	CCNNNNNNNGG	8 cut(s)	310, 370, 454, 835, 1028, 1226, 1409, 1503
BsmAI	GTCTC	1 cut(s)	1247
BsmFI	GGGAC	3 cut(s)	34, 739, 1055
BsmI	GAATGC	3 cut(s)	473, 550, 1439
BsnI	GGCC	5 cut(s)	293, 401, 685, 903, 1881
BsoBI	CYCGRG	2 cut(s)	524, 1295
Bsp119I	TTCGAA	1 cut(s)	692
Bsp1286I	GDGCHC	6 cut(s)	429, 534, 582, 1429, 1830, 1908
Bsp1407I	TGTACA	1 cut(s)	22
Bsp143I	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
Bsp1720I	GCTNAGC	1 cut(s)	608
BspACI	CCGC	9 cut(s)	148, 247, 278, 330, 451, 971, 1267, 1509, 1831
BspANI	GGCC	5 cut(s)	293, 401, 685, 903, 1881
BspCNI	CTCAG	6 cut(s)	409, 600, 988, 1354, 1894, 1920
BspFNI	CGCG	1 cut(s)	148
BspHI	TCATGA	1 cut(s)	324
BspLI	GGNNCC	5 cut(s)	49, 50, 176, 602, 1165
BspMAI	CTGCAG	1 cut(s)	623
BspPI	GGATC	5 cut(s)	181, 846, 1013, 1247, 1574
BspT104I	TTCGAA	1 cut(s)	692
BspTI	CTTAAG	1 cut(s)	1279
BsrDI	GCAATG	1 cut(s)	1141
BsrFI	RCCGGY	2 cut(s)	739, 1894
BsrGI	TGTACA	1 cut(s)	22
BsrI	ACTGG	4 cut(s)	304, 601, 1598, 1772
BssAI	RCCGGY	2 cut(s)	739, 1894
BssECI	CCNNGG	2 cut(s)	384, 834
BssMI	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
BssNI	GRCGYC	2 cut(s)	1077, 1270
BssSI	CACGAG	1 cut(s)	1844
BssT1I	CCWWGG	1 cut(s)	384
Bst2BI	CACGAG	1 cut(s)	1844
Bst2UI	CCWGG	1 cut(s)	900
Bst4CI	ACNGT	3 cut(s)	32, 891, 1810
Bst6I	CTCTTC	2 cut(s)	1535, 1906
BstACI	GRCGYC	2 cut(s)	1077, 1270
BstAFI	CTTAAG	1 cut(s)	1279
BstAPI	GCANNNNNTGC	2 cut(s)	618, 998
BstAUI	TGTACA	1 cut(s)	22
BstBAI	YACGTR	1 cut(s)	1379
BstBI	TTCGAA	1 cut(s)	692
BstC8I	GCNNGC	4 cut(s)	150, 261, 933, 1569
BstDEI	CTNAG	8 cut(s)	396, 608, 858, 975, 1274, 1341, 1902, 1907
BstF5I	GGATG	4 cut(s)	1003, 1126, 1646, 1873
BstFNI	CGCG	1 cut(s)	148
BstH2I	RGCGCY	2 cut(s)	253, 647
BstHHI	GCGC	3 cut(s)	252, 550, 646
BstKTI	GATC	9 cut(s)	189, 288, 337, 466, 841, 1021, 1255, 1466, 1582
BstMAI	GTCTC	1 cut(s)	1247
BstMBI	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
BstNI	CCWGG	1 cut(s)	900
BstNSI	RCATGY	4 cut(s)	782, 1571, 1858, 1890
BstPAI	GACNNNNGTC	1 cut(s)	359
BstSCI	CCNGG	4 cut(s)	834, 898, 903, 1071
BstSFI	CTRYAG	2 cut(s)	223, 619
BstSLI	GKGCMC	2 cut(s)	534, 582
BstUI	CGCG	1 cut(s)	148
BstV1I	GCAGC	1 cut(s)	1711
BstV2I	GAAGAC	3 cut(s)	828, 1751, 1757
BstX2I	RGATCY	2 cut(s)	838, 1252
BstYI	RGATCY	2 cut(s)	838, 1252
Bsu36I	CCTNAGG	1 cut(s)	975
BsuRI	GGCC	5 cut(s)	293, 401, 685, 903, 1881
BtgZI	GCGATG	1 cut(s)	912
BtsCI	GGATG	4 cut(s)	1003, 1126, 1646, 1873
BtsI	GCAGTG	2 cut(s)	619, 628
BtsIMutI	CAGTG	4 cut(s)	619, 628, 867, 1605
Cac8I	GCNNGC	4 cut(s)	150, 261, 933, 1569
CaiI	CAGNNNCTG	1 cut(s)	618
CciI	TCATGA	1 cut(s)	324
CfoI	GCGC	3 cut(s)	252, 550, 646
Cfr10I	RCCGGY	2 cut(s)	739, 1894
Cfr13I	GGNCC	5 cut(s)	48, 400, 457, 902, 1547
CseI	GACGC	4 cut(s)	154, 924, 1085, 1259
Csp6I	GTAC	7 cut(s)	23, 307, 484, 887, 1363, 1645, 1957
CspAI	ACCGGT	2 cut(s)	739, 1894
CviQI	GTAC	7 cut(s)	23, 307, 484, 887, 1363, 1645, 1957
DdeI	CTNAG	8 cut(s)	396, 608, 858, 975, 1274, 1341, 1902, 1907
DpnI	GATC	9 cut(s)	188, 287, 336, 465, 840, 1020, 1254, 1465, 1581
DpnII	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
EaeI	YGGCCR	1 cut(s)	683
Eam1104I	CTCTTC	2 cut(s)	1535, 1906
EarI	CTCTTC	2 cut(s)	1535, 1906
Ecl136II	GAGCTC	2 cut(s)	427, 1906
Eco130I	CCWWGG	1 cut(s)	384
Eco147I	AGGCCT	1 cut(s)	293
Eco24I	GRGCYC	3 cut(s)	429, 1429, 1908
Eco47I	GGWCC	3 cut(s)	48, 457, 1547
Eco47III	AGCGCT	1 cut(s)	645
Eco53kI	GAGCTC	2 cut(s)	427, 1906
Eco81I	CCTNAGG	1 cut(s)	975
Eco88I	CYCGRG	2 cut(s)	524, 1295
EcoICRI	GAGCTC	2 cut(s)	427, 1906
EcoO109I	RGGNCCY	3 cut(s)	48, 400, 457
EcoRI	GAATTC	3 cut(s)	180, 688, 1796
EcoRII	CCWGG	1 cut(s)	898
EcoT14I	CCWWGG	1 cut(s)	384
EcoT22I	ATGCAT	1 cut(s)	1678
EcoT38I	GRGCYC	3 cut(s)	429, 1429, 1908
ErhI	CCWWGG	1 cut(s)	384
FalI	AAGNNNNNCTT	2 cut(s)	473, 505
FaqI	GGGAC	3 cut(s)	34, 739, 1055
FauI	CCCGC	1 cut(s)	141
Fnu4HI	GCNGC	3 cut(s)	248, 1268, 1725
FokI	GGATG	4 cut(s)	1010, 1133, 1653, 1880
FriOI	GRGCYC	3 cut(s)	429, 1429, 1908
Fsp4HI	GCNGC	3 cut(s)	248, 1268, 1725
FspBI	CTAG	2 cut(s)	1757, 1820
GlaI	GCGC	3 cut(s)	251, 549, 645
GluI	GCNGC	3 cut(s)	248, 1268, 1725
GsaI	CCCAGC	1 cut(s)	1215
GsuI	CTGGAG	4 cut(s)	110, 1023, 1671, 1737
HaeII	RGCGCY	2 cut(s)	253, 647
HaeIII	GGCC	5 cut(s)	293, 401, 685, 903, 1881
HapII	CCGG	9 cut(s)	136, 172, 281, 740, 836, 905, 1073, 1526, 1895
HgaI	GACGC	4 cut(s)	154, 924, 1085, 1259
HhaI	GCGC	3 cut(s)	252, 550, 646
Hin1I	GRCGYC	2 cut(s)	1077, 1270
Hin6I	GCGC	3 cut(s)	250, 548, 644
HinP1I	GCGC	3 cut(s)	250, 548, 644
HincII	GTYRAC	1 cut(s)	1384
HindII	GTYRAC	1 cut(s)	1384
HindIII	AAGCTT	1 cut(s)	1970
HinfI	GANTC	6 cut(s)	154, 201, 348, 1385, 1771, 1918
HpaII	CCGG	9 cut(s)	136, 172, 281, 740, 836, 905, 1073, 1526, 1895
HphI	GGTGA	1 cut(s)	676
Hpy166II	GTNNAC	6 cut(s)	532, 1338, 1363, 1384, 1550, 1843
Hpy8I	GTNNAC	6 cut(s)	532, 1338, 1363, 1384, 1550, 1843
Hpy99I	CGWCG	1 cut(s)	148
HpyAV	CCTTC	6 cut(s)	95, 361, 1294, 1609, 1631, 1939
HpyCH4III	ACNGT	3 cut(s)	32, 891, 1810
HpyCH4IV	ACGT	2 cut(s)	627, 1378
HpyF3I	CTNAG	8 cut(s)	396, 608, 858, 975, 1274, 1341, 1902, 1907
HpySE526I	ACGT	2 cut(s)	627, 1378
Hsp92I	GRCGYC	2 cut(s)	1077, 1270
HspAI	GCGC	3 cut(s)	250, 548, 644
KflI	GGGWCCC	1 cut(s)	48
Kzo9I	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
LmnI	GCTCC	2 cut(s)	180, 1833
Lsp1109I	GCAGC	1 cut(s)	1711
LweI	GCATC	4 cut(s)	330, 988, 1467, 1663
MaeI	CTAG	2 cut(s)	1757, 1820
MaeII	ACGT	2 cut(s)	627, 1378
MaeIII	GTNAC	6 cut(s)	93, 614, 623, 865, 936, 1309
MalI	GATC	9 cut(s)	188, 287, 336, 465, 840, 1020, 1254, 1465, 1581
MboI	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
MfeI	CAATTG	1 cut(s)	1089
MflI	RGATCY	2 cut(s)	838, 1252
MhlI	GDGCHC	6 cut(s)	429, 534, 582, 1429, 1830, 1908
MlyI	GAGTC	1 cut(s)	1379
Mph1103I	ATGCAT	1 cut(s)	1678
MseI	TTAA	4 cut(s)	771, 1196, 1280, 1805
MslI	CAYNNNNRTG	2 cut(s)	1671, 1848
MspA1I	CMGCKG	3 cut(s)	247, 607, 1043
MspCI	CTTAAG	1 cut(s)	1279
MspI	CCGG	9 cut(s)	136, 172, 281, 740, 836, 905, 1073, 1526, 1895
MspR9I	CCNGG	4 cut(s)	836, 900, 905, 1073
MunI	CAATTG	1 cut(s)	1089
Mva1269I	GAATGC	3 cut(s)	473, 550, 1439
MvaI	CCWGG	1 cut(s)	900
MvnI	CGCG	1 cut(s)	148
NciI	CCSGG	3 cut(s)	836, 905, 1073
NdeII	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
NlaIV	GGNNCC	5 cut(s)	49, 50, 176, 602, 1165
NmuCI	GTSAC	4 cut(s)	614, 623, 865, 936
NsiI	ATGCAT	1 cut(s)	1678
NspI	RCATGY	4 cut(s)	782, 1571, 1858, 1890
NspV	TTCGAA	1 cut(s)	692
PaeI	GCATGC	1 cut(s)	1571
PaeR7I	CTCGAG	1 cut(s)	1295
PagI	TCATGA	1 cut(s)	324
PceI	AGGCCT	1 cut(s)	293
PciI	ACATGT	1 cut(s)	1854
PctI	GAATGC	3 cut(s)	473, 550, 1439
PfeI	GAWTC	5 cut(s)	154, 201, 348, 1771, 1918
PflFI	GACNNNGTC	1 cut(s)	704
PflMI	CCANNNNNTGG	2 cut(s)	1028, 1409
PfoI	TCCNGGA	1 cut(s)	1071
PinAI	ACCGGT	2 cut(s)	739, 1894
PkrI	GCNGC	3 cut(s)	249, 1269, 1726
PleI	GAGTC	1 cut(s)	1379
PpsI	GAGTC	1 cut(s)	1379
Ppu21I	YACGTR	1 cut(s)	1379
PpuMI	RGGWCCY	2 cut(s)	48, 457
PscI	ACATGT	1 cut(s)	1854
PshAI	GACNNNNGTC	1 cut(s)	359
PsiI	TTATAA	1 cut(s)	1398
Psp124BI	GAGCTC	2 cut(s)	429, 1908
Psp5II	RGGWCCY	2 cut(s)	48, 457
Psp6I	CCWGG	1 cut(s)	898
PspFI	CCCAGC	1 cut(s)	1211
PspGI	CCWGG	1 cut(s)	898
PspN4I	GGNNCC	5 cut(s)	49, 50, 176, 602, 1165
PspPI	GGNCC	5 cut(s)	48, 400, 457, 902, 1547
PspPPI	RGGWCCY	2 cut(s)	48, 457
PstI	CTGCAG	1 cut(s)	623
PstNI	CAGNNNCTG	1 cut(s)	618
PsuI	RGATCY	2 cut(s)	838, 1252
PsyI	GACNNNGTC	1 cut(s)	704
PvuII	CAGCTG	2 cut(s)	607, 1043
RsaI	GTAC	7 cut(s)	24, 308, 485, 888, 1364, 1646, 1958
RsaNI	GTAC	7 cut(s)	23, 307, 484, 887, 1363, 1645, 1957
RseI	CAYNNNNRTG	2 cut(s)	1671, 1848
SacI	GAGCTC	2 cut(s)	429, 1908
SaqAI	TTAA	4 cut(s)	771, 1196, 1280, 1805
SatI	GCNGC	3 cut(s)	248, 1268, 1725
Sau3AI	GATC	9 cut(s)	186, 285, 334, 463, 838, 1018, 1252, 1463, 1579
Sau96I	GGNCC	5 cut(s)	48, 400, 457, 902, 1547
ScaI	AGTACT	1 cut(s)	308
SchI	GAGTC	1 cut(s)	1379
ScrFI	CCNGG	4 cut(s)	836, 900, 905, 1073
SduI	GDGCHC	6 cut(s)	429, 534, 582, 1429, 1830, 1908
SfaNI	GCATC	4 cut(s)	330, 988, 1467, 1663
SfcI	CTRYAG	2 cut(s)	223, 619
Sfr274I	CTCGAG	1 cut(s)	1295
SfuI	TTCGAA	1 cut(s)	692
SgrAI	CRCCGGYG	1 cut(s)	739
SinI	GGWCC	3 cut(s)	48, 457, 1547
SlaI	CTCGAG	1 cut(s)	1295
SmiMI	CAYNNNNRTG	2 cut(s)	1671, 1848
SmlI	CTYRAG	2 cut(s)	1279, 1295
SmoI	CTYRAG	2 cut(s)	1279, 1295
SphI	GCATGC	1 cut(s)	1571
SseBI	AGGCCT	1 cut(s)	293
SsiI	CCGC	9 cut(s)	148, 247, 278, 330, 451, 971, 1267, 1509, 1831
SspI	AATATT	1 cut(s)	1351
SspMI	CTAG	2 cut(s)	1757, 1820
SstI	GAGCTC	2 cut(s)	429, 1908
StuI	AGGCCT	1 cut(s)	293
StyD4I	CCNGG	4 cut(s)	834, 898, 903, 1071
StyI	CCWWGG	1 cut(s)	384
TaaI	ACNGT	3 cut(s)	32, 891, 1810
TaiI	ACGT	2 cut(s)	630, 1381
TatI	WGTACW	3 cut(s)	22, 306, 1956
TauI	GCSGC	2 cut(s)	250, 1270
TfiI	GAWTC	5 cut(s)	154, 201, 348, 1771, 1918
Tru1I	TTAA	4 cut(s)	771, 1196, 1280, 1805
Tru9I	TTAA	4 cut(s)	771, 1196, 1280, 1805
TscAI	CASTG	4 cut(s)	619, 628, 874, 1605
TseFI	GTSAC	4 cut(s)	614, 623, 865, 936
TseI	GCWGC	1 cut(s)	1724
Tsp45I	GTSAC	4 cut(s)	614, 623, 865, 936
TspDTI	ATGAA	7 cut(s)	556, 557, 707, 1128, 1137, 1196, 1754
TspGWI	ACGGA	3 cut(s)	64, 371, 1954
TspRI	CASTG	4 cut(s)	619, 628, 874, 1605
Tth111I	GACNNNGTC	1 cut(s)	704
Van91I	CCANNNNNTGG	2 cut(s)	1028, 1409
Vha464I	CTTAAG	1 cut(s)	1279
VneI	GTGCAC	1 cut(s)	530
VpaK11BI	GGWCC	3 cut(s)	48, 457, 1547
XapI	RAATTY	6 cut(s)	121, 180, 688, 920, 1790, 1796
XceI	RCATGY	4 cut(s)	782, 1571, 1858, 1890
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1410
XhoI	CTCGAG	1 cut(s)	1295
XspI	CTAG	2 cut(s)	1757, 1820
ZrmI	AGTACT	1 cut(s)	308
Zsp2I	ATGCAT	1 cut(s)	1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.