pycom01g23620

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0020388
Physical Location & Seq
Forward (+)
20882201 .. 20883262
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23620.1

Sequence Viewer

Length: 1062 bp
ATGGCGGATGACGATGGAAAATTGCCGATTTTCTCCTCCACCAACGACGATATAATCGTCGAGATACTTTCATGGCTACCAGTCATATGTCTCCTCCGATTCCGTTGTGTATGCAAGTCATGGCGCGCCTTAATTTCCACTTCCGATTTCATCGCCAAGCACCGAGGACGTACCAACTACAACGATAACAATAAGAATATCTTGATCATCTTTAAACGTCCCCCTCTACCTTCCCATCATTTTCGTGATCCGCCATCTCGATTTCCCCAAATCGTTGACTACCAATCACTGAAGAAGAATAACGATGCCTCCGGCGTCTCCGCCTCCGCGTCTGCAGCAGCAATACGAAGCCGACGTAGAGACCTTGAAGATGAATTTCCAGATGATACTAGTCTCAACGATTCATCCCTTGTGGGTTCTTGCAATGGCCTTATCTGCCTACTACTTGACCCTCCACCTAGTGAATGCTATCCAAAATCCTATGAAAATATCAGACTTCTCTTGTGGAACCCTAGTACCAGAAAAGCCCGTAAGTTACCGGACATTGATTTGTTTCCTTTCGGTCCACTTTATTATGGACTTGGCTATGATTCCACCACTCAAGACTACAAGGTCTTTTTGGGCGGTTTTAATTATCCTGATGATCCAGGACTGGTTGCAATCTTTGCACTCAAAACGGGTTCATGGAAGATTGTGGGAGCAGACCACATCGATGCTTCCTTGGTAGAAAGCCGTCGGCGAGGGTGCTTCTTAAATGGAGCTCTACATTGGAATCAGCATGACAGAAGGAACAAGGGAACAAAAATCTGGTCCTTTGATTTCGCACAGGAGAAATTTCGTTCGTTTTCCTTTGTTAGGCATAAAGGATATAGTACAGATGCGGGACTGGGGACTATTGGAAATCTTCTCTTCTTTTACACTTCCTGCCATAGCCTTACAAATCATCATGACTTGGAGGACGCCGGCCTTACCATATGGGTGATGATGGAATATGGGGTCTATCAATCTTGGACTATTTTCGCAAAAATCCGCTCTGAGATTTTCCCTTCAGATGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

354

Amino Acids

40.14

Weight (kDa)

8.05

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 50 2.6e-09 F-box domain
F-box-like PF12937 16 - 48 2.7e-06 F-box-like
FBA_1 PF07734 132 - 342 4.7e-20 F-box associated beta propeller domain
FBA_3 PF08268 137 - 331 5.3e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020388)

Species Orthologous Gene IDs
malus_domestica MD08G1201200.v1.1
pyrus_communis pycom01g23620 pycom08g17460 pycom08g17480 pycom08g20380 pycom08g20390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 611
AccBSI CCGCTC 1 cut(s) 1032
AccII CGCG 2 cut(s) 126, 329
AciI CCGC 7 cut(s) 5, 251, 321, 327, 624, 881, 1030
AclWI GGATC 2 cut(s) 242, 638
AcsI RAATTY 2 cut(s) 374, 833
AcuI CTGAAG 2 cut(s) 311, 1032
AcyI GRCGYC 2 cut(s) 315, 960
AdeI CACNNNGTG 1 cut(s) 461
AfaI GTAC 3 cut(s) 172, 517, 874
AfiI CCNNNNNNNGG 1 cut(s) 855
AgsI TTSAA 1 cut(s) 368
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 646
AluBI AGCT 1 cut(s) 761
AluI AGCT 1 cut(s) 761
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 4 cut(s) 95, 322, 354, 398
AlwI GGATC 2 cut(s) 242, 638
AoxI GGCC 2 cut(s) 427, 964
ApeKI GCWGC 2 cut(s) 335, 338
ApoI RAATTY 2 cut(s) 374, 833
ArsI GACNNNNNNTTYG 2 cut(s) 794, 826
AscI GGCGCGCC 1 cut(s) 124
AspLEI GCGC 2 cut(s) 126, 128
AspS9I GGNCC 2 cut(s) 563, 810
AsuHPI GGTGA 1 cut(s) 991
AvaII GGWCC 2 cut(s) 563, 810
BaeI ACNNNNGTAYC 2 cut(s) 499, 532
BanII GRGCYC 1 cut(s) 763
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 2 cut(s) 347, 350
BccI CCATC 4 cut(s) 8, 243, 262, 979
BceAI ACGGC 1 cut(s) 717
BciT130I CCWGG 1 cut(s) 648
BclI TGATCA 1 cut(s) 204
BcoDI GTCTC 4 cut(s) 95, 322, 354, 398
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 3 cut(s) 390, 459, 513
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 2 cut(s) 336, 339
BlsI GCNGC 2 cut(s) 337, 340
Bme1390I CCNGG 1 cut(s) 648
Bme18I GGWCC 2 cut(s) 563, 810
BmgT120I GGNCC 2 cut(s) 563, 810
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 1 cut(s) 648
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 3 cut(s) 295, 703, 868
BmuI ACTGGG 1 cut(s) 896
BpuEI CTTGAG 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 711
BsaHI GRCGYC 2 cut(s) 315, 960
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 163, 720
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 855
Bse118I RCCGGY 1 cut(s) 962
Bse1I ACTGG 3 cut(s) 80, 657, 891
Bse3DI GCAATG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 648
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 163, 720
BseGI GGATG 2 cut(s) 13, 404
BseLI CCNNNNNNNGG 1 cut(s) 855
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 1026
BseNI ACTGG 3 cut(s) 80, 657, 891
BsePI GCGCGC 1 cut(s) 124
BseRI GAGGAG 2 cut(s) 25, 83
BseXI GCAGC 2 cut(s) 347, 350
Bsh1236I CGCG 2 cut(s) 126, 329
BshFI GGCC 2 cut(s) 429, 966
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 3 cut(s) 312, 539, 963
BslFI GGGAC 3 cut(s) 204, 897, 904
BslI CCNNNNNNNGG 1 cut(s) 855
BsmAI GTCTC 4 cut(s) 95, 322, 354, 398
BsmBI CGTCTC 1 cut(s) 322
BsmFI GGGAC 3 cut(s) 204, 897, 904
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 2 cut(s) 429, 966
Bso31I GGTCTC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 3 cut(s) 204, 247, 643
BspACI CCGC 7 cut(s) 5, 251, 321, 327, 624, 881, 1030
BspANI GGCC 2 cut(s) 429, 966
BspCNI CTCAG 1 cut(s) 1027
BspDI ATCGAT 1 cut(s) 711
BspFNI CGCG 2 cut(s) 126, 329
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 1 cut(s) 509
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 242, 638
BspTNI GGTCTC 1 cut(s) 354
BsrBI CCGCTC 1 cut(s) 1032
BsrDI GCAATG 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 962
BsrI ACTGG 3 cut(s) 80, 657, 891
BssAI RCCGGY 1 cut(s) 962
BssECI CCNNGG 2 cut(s) 163, 720
BssHII GCGCGC 1 cut(s) 124
BssMI GATC 3 cut(s) 204, 247, 643
BssNI GRCGYC 2 cut(s) 315, 960
BssT1I CCWWGG 1 cut(s) 720
Bst2UI CCWGG 1 cut(s) 648
Bst6I CTCTTC 1 cut(s) 914
BstACI GRCGYC 2 cut(s) 315, 960
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 126, 964
BstDEI CTNAG 1 cut(s) 1035
BstENI CCTNNNNNAGG 1 cut(s) 853
BstF5I GGATG 2 cut(s) 13, 404
BstFNI CGCG 2 cut(s) 126, 329
BstHHI GCGC 2 cut(s) 126, 128
BstKTI GATC 3 cut(s) 207, 250, 646
BstMAI GTCTC 4 cut(s) 95, 322, 354, 398
BstMBI GATC 3 cut(s) 204, 247, 643
BstMWI GCNNNNNNNGC 3 cut(s) 335, 435, 665
BstNI CCWGG 1 cut(s) 648
BstSCI CCNGG 1 cut(s) 646
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 2 cut(s) 126, 329
BstV1I GCAGC 2 cut(s) 347, 350
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 2 cut(s) 429, 966
BsuTUI ATCGAT 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 13, 404
BtsIMutI CAGTG 1 cut(s) 287
Cac8I GCNNGC 2 cut(s) 126, 964
CciI TCATGA 1 cut(s) 946
CfoI GCGC 2 cut(s) 126, 128
Cfr10I RCCGGY 1 cut(s) 962
Cfr13I GGNCC 2 cut(s) 563, 810
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 3 cut(s) 304, 318, 968
Csp6I GTAC 3 cut(s) 171, 516, 873
CviAII CATG 5 cut(s) 72, 120, 684, 779, 947
CviJI RGCY 9 cut(s) 76, 351, 429, 527, 585, 732, 761, 933, 966
CviKI_1 RGCY 9 cut(s) 76, 351, 429, 527, 585, 732, 761, 933, 966
CviQI GTAC 3 cut(s) 171, 516, 873
DdeI CTNAG 1 cut(s) 1035
DpnI GATC 3 cut(s) 206, 249, 645
DpnII GATC 3 cut(s) 204, 247, 643
DraI TTTAAA 1 cut(s) 214
DraIII CACNNNGTG 1 cut(s) 461
DrdI GACNNNNNNGTC 1 cut(s) 611
DseDI GACNNNNNNGTC 1 cut(s) 611
Eam1104I CTCTTC 1 cut(s) 914
EarI CTCTTC 1 cut(s) 914
EciI GGCGGA 3 cut(s) 20, 240, 310
Ecl136II GAGCTC 1 cut(s) 761
Eco130I CCWWGG 1 cut(s) 720
Eco24I GRGCYC 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 563, 810
Eco53kI GAGCTC 1 cut(s) 761
Eco57I CTGAAG 2 cut(s) 311, 1032
EcoICRI GAGCTC 1 cut(s) 761
EcoNI CCTNNNNNAGG 1 cut(s) 853
EcoRII CCWGG 1 cut(s) 646
EcoT14I CCWWGG 1 cut(s) 720
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 720
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 5 cut(s) 75, 123, 687, 782, 950
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 3 cut(s) 204, 897, 904
FatI CATG 5 cut(s) 71, 119, 683, 778, 946
FauI CCCGC 1 cut(s) 874
FauNDI CATATG 2 cut(s) 86, 974
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 336, 339
FokI GGATG 2 cut(s) 20, 391
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 2 cut(s) 336, 339
FspBI CTAG 3 cut(s) 390, 459, 513
GlaI GCGC 2 cut(s) 125, 127
GluI GCNGC 2 cut(s) 336, 339
HaeIII GGCC 2 cut(s) 429, 966
HapII CCGG 3 cut(s) 312, 539, 963
HgaI GACGC 3 cut(s) 304, 318, 968
HhaI GCGC 2 cut(s) 126, 128
Hin1I GRCGYC 2 cut(s) 315, 960
Hin1II CATG 5 cut(s) 75, 123, 687, 782, 950
Hin6I GCGC 2 cut(s) 124, 126
HinP1I GCGC 2 cut(s) 124, 126
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 4 cut(s) 99, 401, 590, 772
HpaII CCGG 3 cut(s) 312, 539, 963
HphI GGTGA 1 cut(s) 991
Hpy166II GTNNAC 2 cut(s) 277, 566
Hpy188I TCNGA 5 cut(s) 98, 145, 494, 1036, 1051
Hpy188III TCNNGA 8 cut(s) 61, 202, 245, 258, 380, 602, 638, 947
Hpy8I GTNNAC 2 cut(s) 277, 566
Hpy99I CGWCG 4 cut(s) 50, 62, 357, 738
HpyAV CCTTC 3 cut(s) 240, 780, 1056
HpyCH4IV ACGT 3 cut(s) 169, 217, 355
HpyCH4V TGCA 5 cut(s) 114, 335, 423, 659, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 435, 665
HpyF3I CTNAG 1 cut(s) 1035
HpySE526I ACGT 3 cut(s) 169, 217, 355
Hsp92I GRCGYC 2 cut(s) 315, 960
Hsp92II CATG 5 cut(s) 75, 123, 687, 782, 950
HspAI GCGC 2 cut(s) 124, 126
KroI GCCGGC 1 cut(s) 962
KroNI GCCGGC 1 cut(s) 964
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 3 cut(s) 204, 247, 643
LmnI GCTCC 2 cut(s) 698, 758
Lsp1109I GCAGC 2 cut(s) 347, 350
LweI GCATC 3 cut(s) 295, 703, 868
MaeI CTAG 3 cut(s) 390, 459, 513
MaeII ACGT 3 cut(s) 169, 217, 355
MaeIII GTNAC 1 cut(s) 534
MalI GATC 3 cut(s) 206, 249, 645
MbiI CCGCTC 1 cut(s) 1032
MboI GATC 3 cut(s) 204, 247, 643
MboII GAAGA 6 cut(s) 304, 307, 380, 700, 896, 901
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 5 cut(s) 20, 132, 374, 631, 833
MnlI CCTC 9 cut(s) 46, 104, 158, 234, 319, 334, 462, 734, 949
MroNI GCCGGC 1 cut(s) 962
MseI TTAA 4 cut(s) 131, 213, 630, 752
MslI CAYNNNNRTG 3 cut(s) 243, 711, 977
MspI CCGG 3 cut(s) 312, 539, 963
MspR9I CCNGG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 648
MvnI CGCG 2 cut(s) 126, 329
MwoI GCNNNNNNNGC 3 cut(s) 335, 435, 665
NaeI GCCGGC 1 cut(s) 964
NdeI CATATG 2 cut(s) 86, 974
NdeII GATC 3 cut(s) 204, 247, 643
NgoMIV GCCGGC 1 cut(s) 962
NlaIII CATG 5 cut(s) 75, 123, 687, 782, 950
NlaIV GGNNCC 1 cut(s) 509
PagI TCATGA 1 cut(s) 946
PalAI GGCGCGCC 1 cut(s) 124
PauI GCGCGC 1 cut(s) 124
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 352
PctI GAATGC 1 cut(s) 470
PdiI GCCGGC 1 cut(s) 964
PfeI GAWTC 4 cut(s) 99, 401, 590, 772
PfoI TCCNGGA 1 cut(s) 646
PkrI GCNGC 2 cut(s) 337, 340
Psp124BI GAGCTC 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 646
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 509
PspPI GGNCC 2 cut(s) 563, 810
PstI CTGCAG 1 cut(s) 337
PteI GCGCGC 1 cut(s) 124
RsaI GTAC 3 cut(s) 172, 517, 874
RsaNI GTAC 3 cut(s) 171, 516, 873
RseI CAYNNNNRTG 3 cut(s) 243, 711, 977
SacI GAGCTC 1 cut(s) 763
SaqAI TTAA 4 cut(s) 131, 213, 630, 752
SatI GCNGC 2 cut(s) 336, 339
Sau3AI GATC 3 cut(s) 204, 247, 643
Sau96I GGNCC 2 cut(s) 563, 810
ScrFI CCNGG 1 cut(s) 648
SduI GDGCHC 1 cut(s) 763
SetI ASST 8 cut(s) 172, 220, 232, 358, 366, 460, 615, 763
SfaNI GCATC 3 cut(s) 295, 703, 868
SfcI CTRYAG 1 cut(s) 333
SgsI GGCGCGCC 1 cut(s) 124
SinI GGWCC 2 cut(s) 563, 810
SmiMI CAYNNNNRTG 3 cut(s) 243, 711, 977
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 5 cut(s) 20, 132, 374, 631, 833
SsiI CCGC 7 cut(s) 5, 251, 321, 327, 624, 881, 1030
SspMI CTAG 3 cut(s) 390, 459, 513
SstI GAGCTC 1 cut(s) 763
StyD4I CCNGG 1 cut(s) 646
StyI CCWWGG 1 cut(s) 720
TaiI ACGT 3 cut(s) 172, 220, 358
TaqI TCGA 3 cut(s) 60, 259, 711
TaqII GACCGA 1 cut(s) 551
TasI AATT 5 cut(s) 20, 132, 374, 631, 833
TatI WGTACW 1 cut(s) 872
TfiI GAWTC 4 cut(s) 99, 401, 590, 772
Tru1I TTAA 4 cut(s) 131, 213, 630, 752
Tru9I TTAA 4 cut(s) 131, 213, 630, 752
TscAI CASTG 1 cut(s) 294
TseI GCWGC 2 cut(s) 335, 338
TspDTI ATGAA 6 cut(s) 60, 139, 387, 393, 498, 672
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 563, 810
XagI CCTNNNNNAGG 1 cut(s) 853
XapI RAATTY 2 cut(s) 374, 833
XspI CTAG 3 cut(s) 390, 459, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.