pycom08g17460

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0020388
Physical Location & Seq
Forward (+)
17641482 .. 17642640
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17460.1

Sequence Viewer

Length: 1092 bp
ATGGCGGGTGATGATGGGGAATTGCCGATCTTGTCCTCCAACAATGACGATATAATCGTCGAGATACTTTCATGGCTACCGGTCGTATCTCTCCTCCGATTCCGTTGTGTATGCAAGTCATGGCGCGCCTTAATCACTACTCCCCAGTTCGTGGCCAAGCACCGAGCACATACCAACGACAGCAACAAGAATGTCCTGATTCTCACGAAACCGTACTGGCCGCCACCAGCCCCCCTCAAGGATTCATCACTTGTGGGTTCTTGCAATGGTCTCATCTGCCTACTACTTAACCCTCCACTTGCATACTATGAAGAATACTATGAAAATATAAGACTTCTCTTGTGGAACCCTAGTACCAGAACAGCCCGTAAGGTACCGGACATTGATTGGTTTTGTTTCAGACCATGTTATTACGGATTCGGCTACGATTCCACCACTCAAGACTACAAGGTCCTTTTGGGCGGTCTAAGTGCCAAAGATGGATTTGCTGCAATCTTCTCACTCAAAGCGGCTTCATGGAAGATTGTTGTAGACTCCCTCAAGCCTGCGGCCTCGTTTGGTATTGATGGACGAGGGTGCTTCTTAAATGGAGCTCTACATTGGAATCATAGACACGATAACGGGACAATAATCAGGTCCTTTGATTTCGCACAGGAGAAATTTCATTCGTTTTCCTCTATTAGGCATAACAAATGTAATTGGGCGGGAGTGGGGACTACTGGAAATCGTCTCTTCTTTCACACTTTCTGCCCTGATTCCCAAAATTTTCCTGACTTTGAGGACACTGGCCTTACCCTATGGGTGATGATGGAATATGGGGTCGATCAATCTTGGACTATTTTTGCAAAAATTCGCCCGGAGATTTTCCCTCCTAATGTGTGGTCGTTGAATCCTTTATTCCTCGACGATGGTGCACTTTTGATGGAATCGGATCGAGGTGACTTGGTATTATATATGCCTAAGGAAGATACATGCAGGAATGTGCTCGACAATTCTGATGTTGAAAAATTGAGAGCTTCGTCTTCGTCTGGGCGTGCAGTAATGGTTATGTACGTAGGCACTTTGGTTTCACCGGCGACAGGTGGCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

364

Amino Acids

40.74

Weight (kDa)

6.07

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 15 - 50 1.2e-10 F-box domain
F-box-like PF12937 16 - 49 1.6e-07 F-box-like
FBA_1 PF07734 79 - 357 1.8e-20 F-box associated beta propeller domain
FBA_3 PF08268 87 - 239 3.6e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020388)

Species Orthologous Gene IDs
malus_domestica MD08G1201200.v1.1
pyrus_communis pycom01g23620 pycom08g17460 pycom08g17480 pycom08g20380 pycom08g20390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 449
Acc65I GGTACC 1 cut(s) 373
AccB1I GGYRCC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 151
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 126
AciI CCGC 7 cut(s) 5, 221, 462, 509, 548, 704, 1086
AclWI GGATC 1 cut(s) 939
AcoI YGGCCR 2 cut(s) 153, 218
AcsI RAATTY 3 cut(s) 659, 763, 849
AfaI GTAC 4 cut(s) 215, 355, 375, 1052
AfiI CCNNNNNNNGG 4 cut(s) 151, 238, 681, 1079
AgeI ACCGGT 1 cut(s) 79
AgsI TTSAA 2 cut(s) 889, 1004
AluBI AGCT 2 cut(s) 593, 1016
AluI AGCT 2 cut(s) 593, 1016
Alw21I GWGCWC 4 cut(s) 169, 595, 916, 987
Alw26I GTCTC 2 cut(s) 275, 734
Alw44I GTGCAC 1 cut(s) 912
AlwI GGATC 1 cut(s) 939
AoxI GGCC 4 cut(s) 153, 218, 549, 787
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 3 cut(s) 659, 763, 849
AscI GGCGCGCC 1 cut(s) 124
AsiGI ACCGGT 1 cut(s) 79
Asp718I GGTACC 1 cut(s) 373
AspLEI GCGC 2 cut(s) 126, 128
AspS9I GGNCC 2 cut(s) 451, 636
AsuC2I CCSGG 1 cut(s) 857
AsuHPI GGTGA 4 cut(s) 20, 814, 950, 1062
AvaII GGWCC 2 cut(s) 451, 636
AxyI CCTNAGG 1 cut(s) 960
BaeGI GKGCMC 1 cut(s) 916
BaeI ACNNNNGTAYC 2 cut(s) 337, 370
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 1014
Bbv12I GWGCWC 4 cut(s) 169, 595, 916, 987
BbvI GCAGC 1 cut(s) 475
BccI CCATC 6 cut(s) 8, 473, 560, 802, 902, 916
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BcnI CCSGG 1 cut(s) 857
BcoDI GTCTC 2 cut(s) 275, 734
BfaI CTAG 2 cut(s) 351, 1090
BisI GCNGC 5 cut(s) 221, 489, 510, 549, 1087
BlsI GCNGC 5 cut(s) 222, 490, 511, 550, 1088
Bme1390I CCNGG 1 cut(s) 857
Bme18I GGWCC 2 cut(s) 451, 636
BmgT120I GGNCC 2 cut(s) 451, 636
BmiI GGNNCC 2 cut(s) 347, 375
BmrFI CCNGG 1 cut(s) 857
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 1014
BpuEI CTTGAG 3 cut(s) 221, 423, 524
BpuMI CCSGG 1 cut(s) 857
BsaAI YACGTR 1 cut(s) 1054
BsaI GGTCTC 1 cut(s) 275
BsaWI WCCGGW 2 cut(s) 79, 376
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 238, 681, 1079
Bse118I RCCGGY 2 cut(s) 79, 1072
Bse1I ACTGG 4 cut(s) 145, 221, 724, 790
Bse21I CCTNAGG 1 cut(s) 960
Bse3DI GCAATG 1 cut(s) 271
BseLI CCNNNNNNNGG 4 cut(s) 151, 238, 681, 1079
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 4 cut(s) 145, 221, 724, 790
BsePI GCGCGC 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 1 cut(s) 475
BsgI GTGCAG 1 cut(s) 1056
Bsh1236I CGCG 1 cut(s) 126
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 155, 220, 551, 789
BshNI GGYRCC 1 cut(s) 373
BshTI ACCGGT 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 4 cut(s) 169, 595, 916, 987
BsiSI CCGG 4 cut(s) 80, 377, 857, 1073
BslFI GGGAC 2 cut(s) 637, 727
BslI CCNNNNNNNGG 4 cut(s) 151, 238, 681, 1079
BsmAI GTCTC 2 cut(s) 275, 734
BsmBI CGTCTC 1 cut(s) 734
BsmFI GGGAC 2 cut(s) 637, 727
BsnI GGCC 4 cut(s) 155, 220, 551, 789
Bso31I GGTCTC 1 cut(s) 275
Bsp1286I GDGCHC 4 cut(s) 169, 595, 916, 987
Bsp143I GATC 3 cut(s) 27, 823, 931
BspACI CCGC 7 cut(s) 5, 221, 462, 509, 548, 704, 1086
BspANI GGCC 4 cut(s) 155, 220, 551, 789
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 347, 375
BspPI GGATC 1 cut(s) 939
BspT107I GGYRCC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 2 cut(s) 79, 1072
BsrI ACTGG 4 cut(s) 145, 221, 724, 790
BssAI RCCGGY 2 cut(s) 79, 1072
BssHII GCGCGC 1 cut(s) 124
BssMI GATC 3 cut(s) 27, 823, 931
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 1054
BstC8I GCNNGC 3 cut(s) 126, 546, 1035
BstDEI CTNAG 2 cut(s) 467, 960
BstENI CCTNNNNNAGG 1 cut(s) 679
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 2 cut(s) 126, 128
BstKTI GATC 3 cut(s) 30, 826, 934
BstMAI GTCTC 2 cut(s) 275, 734
BstMBI GATC 3 cut(s) 27, 823, 931
BstMCI CGRYCG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 975
BstSCI CCNGG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 916
BstSNI TACGTA 1 cut(s) 1054
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 1 cut(s) 1014
Bsu36I CCTNAGG 1 cut(s) 960
BsuRI GGCC 4 cut(s) 155, 220, 551, 789
BtsIMutI CAGTG 1 cut(s) 783
Cac8I GCNNGC 3 cut(s) 126, 546, 1035
CfoI GCGC 2 cut(s) 126, 128
Cfr10I RCCGGY 2 cut(s) 79, 1072
Cfr13I GGNCC 2 cut(s) 451, 636
Csp6I GTAC 4 cut(s) 214, 354, 374, 1051
CspAI ACCGGT 1 cut(s) 79
CviAII CATG 5 cut(s) 72, 120, 405, 516, 972
CviQI GTAC 4 cut(s) 214, 354, 374, 1051
DdeI CTNAG 2 cut(s) 467, 960
DpnI GATC 3 cut(s) 29, 825, 933
DpnII GATC 3 cut(s) 27, 823, 931
DrdI GACNNNNNNGTC 1 cut(s) 449
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 2 cut(s) 153, 218
Eam1104I CTCTTC 1 cut(s) 737
EarI CTCTTC 1 cut(s) 737
Ecl136II GAGCTC 1 cut(s) 593
Eco105I TACGTA 1 cut(s) 1054
Eco24I GRGCYC 1 cut(s) 595
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 451, 636
Eco53kI GAGCTC 1 cut(s) 593
Eco81I CCTNAGG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 593
EcoNI CCTNNNNNAGG 1 cut(s) 679
EcoO109I RGGNCCY 2 cut(s) 451, 636
EcoT38I GRGCYC 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 5 cut(s) 75, 123, 408, 519, 975
FaqI GGGAC 2 cut(s) 637, 727
FatI CATG 5 cut(s) 71, 119, 404, 515, 971
FauI CCCGC 1 cut(s) 697
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 5 cut(s) 221, 489, 510, 549, 1087
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 5 cut(s) 221, 489, 510, 549, 1087
FspBI CTAG 2 cut(s) 351, 1090
GlaI GCGC 2 cut(s) 125, 127
GluI GCNGC 5 cut(s) 221, 489, 510, 549, 1087
HaeIII GGCC 4 cut(s) 155, 220, 551, 789
HapII CCGG 4 cut(s) 80, 377, 857, 1073
HhaI GCGC 2 cut(s) 126, 128
Hin1II CATG 5 cut(s) 75, 123, 408, 519, 975
Hin6I GCGC 2 cut(s) 124, 126
HinP1I GCGC 2 cut(s) 124, 126
HpaII CCGG 4 cut(s) 80, 377, 857, 1073
HphI GGTGA 4 cut(s) 20, 814, 950, 1062
Hpy166II GTNNAC 2 cut(s) 532, 914
Hpy188I TCNGA 4 cut(s) 98, 401, 931, 997
Hpy188III TCNNGA 5 cut(s) 61, 196, 205, 440, 770
Hpy8I GTNNAC 2 cut(s) 532, 914
Hpy99I CGWCG 2 cut(s) 62, 908
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 1 cut(s) 1053
HpyCH4V TGCA 8 cut(s) 114, 264, 302, 491, 845, 914, 975, 1037
HpyF3I CTNAG 2 cut(s) 467, 960
HpySE526I ACGT 1 cut(s) 1053
Hsp92II CATG 5 cut(s) 75, 123, 408, 519, 975
HspAI GCGC 2 cut(s) 124, 126
KpnI GGTACC 1 cut(s) 377
Kzo9I GATC 3 cut(s) 27, 823, 931
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 1 cut(s) 475
MaeI CTAG 2 cut(s) 351, 1090
MaeII ACGT 1 cut(s) 1053
MaeIII GTNAC 1 cut(s) 938
MalI GATC 3 cut(s) 29, 825, 933
MboI GATC 3 cut(s) 27, 823, 931
MboII GAAGA 6 cut(s) 323, 487, 532, 724, 977, 1014
MhlI GDGCHC 4 cut(s) 169, 595, 916, 987
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 7 cut(s) 20, 659, 697, 763, 849, 991, 1007
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 63
Mox20I TGGCCA 1 cut(s) 155
MscI TGGCCA 1 cut(s) 155
MseI TTAA 3 cut(s) 131, 288, 584
Msp20I TGGCCA 1 cut(s) 155
MspI CCGG 4 cut(s) 80, 377, 857, 1073
MspR9I CCNGG 1 cut(s) 857
MvnI CGCG 1 cut(s) 126
NciI CCSGG 1 cut(s) 857
NdeII GATC 3 cut(s) 27, 823, 931
NlaIII CATG 5 cut(s) 75, 123, 408, 519, 975
NlaIV GGNNCC 2 cut(s) 347, 375
NmuCI GTSAC 1 cut(s) 938
NspI RCATGY 1 cut(s) 975
PalAI GGCGCGCC 1 cut(s) 124
PauI GCGCGC 1 cut(s) 124
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 9 cut(s) 99, 199, 242, 417, 428, 604, 755, 889, 926
PflMI CCANNNNNTGG 1 cut(s) 151
PinAI ACCGGT 1 cut(s) 79
PkrI GCNGC 5 cut(s) 222, 490, 511, 550, 1088
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
Ppu21I YACGTR 1 cut(s) 1054
PpuMI RGGWCCY 2 cut(s) 451, 636
Psp124BI GAGCTC 1 cut(s) 595
Psp5II RGGWCCY 2 cut(s) 451, 636
PspN4I GGNNCC 2 cut(s) 347, 375
PspPI GGNCC 2 cut(s) 451, 636
PspPPI RGGWCCY 2 cut(s) 451, 636
PteI GCGCGC 1 cut(s) 124
RsaI GTAC 4 cut(s) 215, 355, 375, 1052
RsaNI GTAC 4 cut(s) 214, 354, 374, 1051
SacI GAGCTC 1 cut(s) 595
SaqAI TTAA 3 cut(s) 131, 288, 584
SatI GCNGC 5 cut(s) 221, 489, 510, 549, 1087
Sau3AI GATC 3 cut(s) 27, 823, 931
Sau96I GGNCC 2 cut(s) 451, 636
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 4 cut(s) 169, 595, 916, 987
SetI ASST 8 cut(s) 375, 453, 595, 638, 940, 1018, 1056, 1084
SgrAI CRCCGGYG 1 cut(s) 1072
SgsI GGCGCGCC 1 cut(s) 124
SinI GGWCC 2 cut(s) 451, 636
SmlI CTYRAG 3 cut(s) 236, 438, 539
SmoI CTYRAG 3 cut(s) 236, 438, 539
SnaBI TACGTA 1 cut(s) 1054
Sse9I AATT 7 cut(s) 20, 659, 697, 763, 849, 991, 1007
SsiI CCGC 7 cut(s) 5, 221, 462, 509, 548, 704, 1086
SspMI CTAG 2 cut(s) 351, 1090
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 855
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 1 cut(s) 1056
TaqI TCGA 5 cut(s) 60, 822, 903, 934, 987
TasI AATT 7 cut(s) 20, 659, 697, 763, 849, 991, 1007
TauI GCSGC 4 cut(s) 223, 512, 551, 1089
TfiI GAWTC 9 cut(s) 99, 199, 242, 417, 428, 604, 755, 889, 926
Tru1I TTAA 3 cut(s) 131, 288, 584
Tru9I TTAA 3 cut(s) 131, 288, 584
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 1 cut(s) 488
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 6 cut(s) 60, 234, 324, 336, 504, 653
TspGWI ACGGA 2 cut(s) 92, 429
TspRI CASTG 1 cut(s) 790
Van91I CCANNNNNTGG 1 cut(s) 151
VneI GTGCAC 1 cut(s) 912
VpaK11BI GGWCC 2 cut(s) 451, 636
XagI CCTNNNNNAGG 1 cut(s) 679
XapI RAATTY 3 cut(s) 659, 763, 849
XceI RCATGY 1 cut(s) 975
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 2 cut(s) 351, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.