pycom02g00630

Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010668
Physical Location & Seq
Reverse (-)
347097 .. 349138
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGGCGAGAGGACCTCTCGCTTTTCAGCTATGGATCTCTGTGTGTCTTTTGCTCTTCTTTCATGCTCGCTGCTTCTACCTCCCGGGTGTCGCCCCTCAAGATTTTCAGCAGGGAAATACCTTGAATGTGAAGGTGAACAAACTGACCTCTACAAAAACTCAACTTCCTTACTCATACTATTCCCTCCCATACTGTTCTCCGGAGCATATAGTAGACAGTGCAGAAAATCTTGGGGAAGTTCTTCGTGGTGATCGCATTGAAAACTCTCCTTACGAGTTCAACATGAGAGAACCAAAGATGTGCAGTGTTGTATGTCGTGTAGTTCTTAATGCAAAAACTGCAAAGGAGTTTAAGGAAAAGATAGATGATGAGTATCGGGTGAACATGATTCTGGATAATCTTCCTCTGGTTGTTCCTATACCACGGCTTGATCGGGAAAATGCCTTAGTGTATCAACATGGATTTCATGTTGGTCTTAGAGGACAATATGCTGGGAACAAGGATGAAAAGCATTTTATCAACAATCACTTAACATTTACAGTCAAGTATCACAAGGATCCAATGACAGAATCTGCCAGGATTGTCGGATTTGAGGTGAAACCATTCAGTGTTAAGCATGAATATGAAGGTGAGTGGACTAACAAGACACGTTTAACGACCTGTGATCCCCATGCAAAGCGAACAGTTAGCAGCTCTGAATCTCCTCAGGAGGTTGAAGATAAAAAGGAAATTATATTCACATATGATGTTGAGTTCCAGGAGAGTGATGTGAAGTGGGCATCCCGGTGGGACACATATCTACTGATGGCTGATGATCAGATCGTAGCACTGGTTCTCAATTGTTAA

Protein Analysis

282

Amino Acids

32.53

Weight (kDa)

6.01

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP70 PF02990 56 - 274 9.6e-58 Endomembrane protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010668)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24170 AT2G24170
fragaria_vesca FvH4_1g00890 FvH4_1g00890 FvH4_1g00890
malus_domestica MD02G1007300.v1.1 MD15G1144600.v1.1
prunus_persica Prupe.7G263100_v2.0.a1
pyrus_communis pycom02g00630 pycom15g13620
rosa_chinensis RchiOBHm_Chr2g0085601
rosa_laevigata RLG00000015704
rosa_multiflora Rmu_sc0016087.1_g000003
rosa_roxburghii Rroxscaffold_2G00155230
rosa_rugosa Rorug01G0461500
rosa_samantha Rh2AG010800 Rh2BG011200 Rh2CG012100 Rh2DG013200
rosa_wichuraiana Rw2G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AccIII TCCGGA 1 cut(s) 199
AclWI GGATC 4 cut(s) 41, 551, 564, 659
AflIII ACRYGT 1 cut(s) 647
AgsI TTSAA 4 cut(s) 124, 260, 280, 716
AjnI CCWGG 2 cut(s) 575, 756
AluBI AGCT 2 cut(s) 28, 693
AluI AGCT 2 cut(s) 28, 693
AlwI GGATC 4 cut(s) 41, 551, 564, 659
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 82
Aor13HI TCCGGA 1 cut(s) 199
ApeKI GCWGC 2 cut(s) 69, 690
Asp700I GAANNNNTTC 2 cut(s) 240, 602
AspS9I GGNCC 1 cut(s) 11
AsuC2I CCSGG 3 cut(s) 83, 84, 784
AsuHPI GGTGA 5 cut(s) 145, 260, 391, 607, 641
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 1 cut(s) 11
AxyI CCTNAGG 1 cut(s) 705
BamHI GGATCC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 56, 702
BccI CCATC 1 cut(s) 799
BceAI ACGGC 1 cut(s) 440
BciT130I CCWGG 2 cut(s) 577, 758
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 3 cut(s) 83, 84, 784
BisI GCNGC 2 cut(s) 70, 691
BlsI GCNGC 2 cut(s) 71, 692
Bme1390I CCNGG 5 cut(s) 83, 84, 577, 758, 784
Bme18I GGWCC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 5 cut(s) 83, 84, 577, 758, 784
BmsI GCATC 1 cut(s) 788
BplI GAGNNNNNCTC 2 cut(s) 30, 32
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 3 cut(s) 83, 84, 784
BsaBI GATNNNNATC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 82, 422
BsaWI WCCGGW 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 834
Bse21I CCTNAGG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 2 cut(s) 577, 758
BseDI CCNNGG 2 cut(s) 82, 422
BseGI GGATG 2 cut(s) 508, 779
BseJI GATNNNNATC 1 cut(s) 372
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 1 cut(s) 693
BseXI GCAGC 2 cut(s) 56, 702
BseYI CCCAGC 1 cut(s) 491
BsgI GTGCAG 2 cut(s) 240, 322
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 3 cut(s) 83, 200, 784
BslFI GGGAC 1 cut(s) 803
BsmFI GGGAC 1 cut(s) 803
BsoBI CYCGRG 1 cut(s) 82
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
BspCNI CTCAG 1 cut(s) 718
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 558
BspPI GGATC 4 cut(s) 41, 551, 564, 659
BspQI GCTCTTC 1 cut(s) 59
BsrI ACTGG 1 cut(s) 834
BssECI CCNNGG 2 cut(s) 82, 422
BssMI GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
Bst2UI CCWGG 2 cut(s) 577, 758
Bst4CI ACNGT 4 cut(s) 194, 218, 541, 685
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 3 cut(s) 445, 476, 705
BstDSI CCRYGG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 508, 779
BstKTI GATC 7 cut(s) 36, 253, 433, 559, 667, 817, 822
BstMBI GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 2 cut(s) 577, 758
BstSCI CCNGG 5 cut(s) 81, 82, 575, 756, 782
BstV1I GCAGC 2 cut(s) 56, 702
BstX2I RGATCY 2 cut(s) 33, 556
BstYI RGATCY 2 cut(s) 33, 556
Bsu36I CCTNAGG 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 2 cut(s) 508, 779
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 4 cut(s) 223, 310, 613, 827
Cac8I GCNNGC 1 cut(s) 67
CaiI CAGNNNCTG 1 cut(s) 572
Cfr13I GGNCC 1 cut(s) 11
Cfr9I CCCGGG 1 cut(s) 82
CviAII CATG 7 cut(s) 62, 283, 385, 458, 467, 617, 671
CviJI RGCY 4 cut(s) 28, 427, 693, 809
CviKI_1 RGCY 4 cut(s) 28, 427, 693, 809
DdeI CTNAG 3 cut(s) 445, 476, 705
DpnI GATC 7 cut(s) 35, 252, 432, 558, 666, 816, 821
DpnII GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 11
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 575, 756
FaeI CATG 7 cut(s) 65, 286, 388, 461, 470, 620, 674
FaqI GGGAC 1 cut(s) 803
FatI CATG 7 cut(s) 61, 282, 384, 457, 466, 616, 670
FauNDI CATATG 1 cut(s) 742
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 2 cut(s) 70, 691
FokI GGATG 2 cut(s) 515, 766
Fsp4HI GCNGC 2 cut(s) 70, 691
GluI GCNGC 2 cut(s) 70, 691
GsaI CCCAGC 1 cut(s) 495
HapII CCGG 3 cut(s) 83, 200, 784
Hin1II CATG 7 cut(s) 65, 286, 388, 461, 470, 620, 674
HinfI GANTC 3 cut(s) 388, 569, 698
HpaII CCGG 3 cut(s) 83, 200, 784
HphI GGTGA 5 cut(s) 145, 260, 391, 607, 641
Hpy166II GTNNAC 4 cut(s) 136, 214, 382, 636
Hpy188I TCNGA 3 cut(s) 587, 697, 819
Hpy188III TCNNGA 5 cut(s) 98, 200, 392, 434, 707
Hpy8I GTNNAC 4 cut(s) 136, 214, 382, 636
HpyAV CCTTC 2 cut(s) 124, 620
HpyCH4III ACNGT 4 cut(s) 194, 218, 541, 685
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 5 cut(s) 221, 303, 332, 341, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 445, 476, 705
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 7 cut(s) 65, 286, 388, 461, 470, 620, 674
Kpn2I TCCGGA 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 2 cut(s) 56, 702
LweI GCATC 1 cut(s) 788
MaeII ACGT 1 cut(s) 649
MalI GATC 7 cut(s) 35, 252, 432, 558, 666, 816, 821
MboI GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
MboII GAAGA 4 cut(s) 46, 233, 392, 728
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 2 cut(s) 33, 556
MluCI AATT 2 cut(s) 729, 838
MmeI TCCRAC 1 cut(s) 565
MroI TCCGGA 1 cut(s) 199
MroXI GAANNNNTTC 2 cut(s) 240, 602
MseI TTAA 6 cut(s) 327, 351, 530, 612, 653, 844
MslI CAYNNNNRTG 2 cut(s) 621, 784
MspI CCGG 3 cut(s) 83, 200, 784
MspR9I CCNGG 5 cut(s) 83, 84, 577, 758, 784
MunI CAATTG 1 cut(s) 838
MvaI CCWGG 2 cut(s) 577, 758
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 3 cut(s) 83, 84, 784
NdeI CATATG 1 cut(s) 742
NdeII GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
NlaIII CATG 7 cut(s) 65, 286, 388, 461, 470, 620, 674
NlaIV GGNNCC 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 59
PdmI GAANNNNTTC 2 cut(s) 240, 602
PfeI GAWTC 3 cut(s) 388, 569, 698
PfoI TCCNGGA 1 cut(s) 756
PkrI GCNGC 2 cut(s) 71, 692
PpuMI RGGWCCY 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 575, 756
PspFI CCCAGC 1 cut(s) 491
PspGI CCWGG 2 cut(s) 575, 756
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PsrI GAACNNNNNNTAC 1 cut(s) 816
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 2 cut(s) 33, 556
RseI CAYNNNNRTG 2 cut(s) 621, 784
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 6 cut(s) 327, 351, 530, 612, 653, 844
SatI GCNGC 2 cut(s) 70, 691
Sau3AI GATC 7 cut(s) 33, 250, 430, 556, 664, 814, 819
Sau96I GGNCC 1 cut(s) 11
ScrFI CCNGG 5 cut(s) 83, 84, 577, 758, 784
SfaNI GCATC 1 cut(s) 788
SinI GGWCC 1 cut(s) 11
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 2 cut(s) 621, 784
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 2 cut(s) 729, 838
StyD4I CCNGG 5 cut(s) 81, 82, 575, 756, 782
TaaI ACNGT 4 cut(s) 194, 218, 541, 685
TaiI ACGT 1 cut(s) 652
TasI AATT 2 cut(s) 729, 838
TfiI GAWTC 3 cut(s) 388, 569, 698
Tru1I TTAA 6 cut(s) 327, 351, 530, 612, 653, 844
Tru9I TTAA 6 cut(s) 327, 351, 530, 612, 653, 844
TscAI CASTG 4 cut(s) 223, 310, 613, 834
TseI GCWGC 2 cut(s) 69, 690
TspDTI ATGAA 5 cut(s) 50, 455, 519, 633, 639
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 4 cut(s) 223, 310, 613, 834
VpaK11BI GGWCC 1 cut(s) 11
XmaI CCCGGG 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 2 cut(s) 240, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.