pycom02g02290

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013578
Physical Location & Seq
Forward (+)
1407119 .. 1408833
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
ATGAAAAACGTCACGGCGGCGGTGGCGACTGTGTCGAGGCGTTTGAGCAAAACCCACGATTGGATTGTGGCTCTCAAGGTCCTTATGCTTGTTCATAGGGTTTTGGTTGATGGGCACCCTTCGTTTGCGGAGGAGATTGTGTCTGCAAGTCGGATAGGAGTGCGGTTTCTGAATTTGTCGGCTTTTCGTGACGAGGCGCATTCGGGTTCTTGGGATCATGCCGGGTTTGTGAGGTTTTATGCCATGTATCTTGAGGAGAAGGTGGAGTTTGAGGTTTATGAGAGGAAATCGAGAGGCAGGGAGGGCAGTAGTGGATTCGATCAGGAGAGGGAGAGAGCGAATCGAGAGGAAAGGAGGGAATTTGAGTACGAAAATTATCAGAGTGATCAGGCATTGGAGAGAGAGAAGAGAAGAGAGAGTAAGGATAGTACACCGGTGAGGGAAATGCGGCCGGAGAGGGTGTTGGTGAGGCTGAATGATTTGCTGAGGGTTCTTAATCGGATTTTGGGTTGCAGGCCGGTGGGTGCTGCAAAGAGTAGTAGGTTGGTGATCGTTGCGCTATACGAGGTTGTGAAGGAGAGTTTTAGGCTGTATGTTGAGATATGTGAGGCATTGGGGGTGGTGCTGGATAGGTTTACTGAGATGGATTATGCGGATTGCGCTAAGGCTTTCGATGCTTATGTCAATGGGGCAAAGATGATTGATGAGCTTGTGGGTTTTTATGGTTGGTGTAAGGATATGGGAATTGCACGTTCGTCTGAGTTCCCGGAGGTGCAGAGAATAACTGATAAGCTTTTGAAGTCGCTTGAGGGTTTCTTGAAGGGGAAGACAACGAGGCCAAAGAGTCCTGAGAGAAACAGGGAGGAGAATGTTCAGATTGACGAGGAGGCGAAGCAGGATGTGAATTATGAGGTGAATGCTCTTCCTCCGCCAGAGATTTACACTGCTTCTCCTCCCCTGCCATCAGAAGTCCGGCCTAAGCCTTTGCAGCATCAGCCTGTGACAGAGGATTTGGTGAATTTGAGGGATGATGGAATCACAGCTGATGAGCAAGGAAACAAATTCGCTTTGGCTTTGTTCTCTGCGGCTCCTACTGCTACTAATACAAATGGGGCCTGGGAAACATTCCCTTCAAATGGAGAGCCTGAAATGACTTCGGCTTGGCAGACACCAGCTGCTGAGAGTGGTAAAGCAGACTGGGAATTGGCATTGGTGGAGACGGCGAGTAATCTGTCAAAACAGAAGGCTGATTTGGCTGGTGGTTTTGATTCATTGTTATTGAGTGGCATGTATGATCAAGGCGCTGTGAAGCACCATGTGAGCGCCTCGCAATTGAGTGGTGGGAGTGCGAGTAGCGTGGCAATGCCGGGGGCAGGCAAGTCCGCTACACCAGTGCTGGCTCTGCCTGCCCCGGATGGGACTGTCCACGCAGTGGGGCAGCAGGACCCGTTTGCCGCCTCCCTCTTAGTGCCTCCTCCGTCATACGTGCAGATGGCAGACATGGAGAGGAAGCAACATTTGCTTTCACAGGAACAACTGCTTTGGCAGCAATATGGAAGAGATGGGATGCAAGGGCAGTTGGGTTTGGCTAAGATTTCTGGCGGCTACTATGGCAGTGCTCCTCAACCGATGATGCCTCAGCCGGGAGGGTACTACTATGCGCCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

557

Amino Acids

61.77

Weight (kDa)

5.46

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 3 - 274 1.9e-57 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 114
AccB7I CCANNNNNTGG 1 cut(s) 1432
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 1 cut(s) 449
AcsI RAATTY 4 cut(s) 172, 359, 1018, 1061
AdeI CACNNNGTG 2 cut(s) 1318, 1432
AfaI GTAC 3 cut(s) 368, 430, 1652
AfiI CCNNNNNNNGG 6 cut(s) 60, 1136, 1373, 1416, 1432, 1643
AgeI ACCGGT 1 cut(s) 433
AgsI TTSAA 3 cut(s) 799, 820, 1134
AjnI CCWGG 1 cut(s) 1115
AjuI GAANNNNNNNTTGG 2 cut(s) 1235, 1267
AluBI AGCT 4 cut(s) 709, 793, 1043, 1175
AluI AGCT 4 cut(s) 709, 793, 1043, 1175
Alw21I GWGCWC 1 cut(s) 1621
Alw26I GTCTC 1 cut(s) 1211
AlwI GGATC 1 cut(s) 222
AlwNI CAGNNNCTG 1 cut(s) 1178
AoxI GGCC 5 cut(s) 449, 515, 836, 974, 1113
ApeKI GCWGC 5 cut(s) 527, 988, 1175, 1438, 1546
ApoI RAATTY 4 cut(s) 172, 359, 1018, 1061
ArsI GACNNNNNNTTYG 2 cut(s) 994, 1026
AsiGI ACCGGT 1 cut(s) 433
AspLEI GCGC 6 cut(s) 199, 559, 662, 1304, 1325, 1663
AspS9I GGNCC 3 cut(s) 79, 1113, 1444
AsuC2I CCSGG 5 cut(s) 223, 767, 1368, 1412, 1644
AsuHPI GGTGA 5 cut(s) 448, 478, 559, 925, 1027
AvaII GGWCC 2 cut(s) 79, 1444
BaeGI GKGCMC 1 cut(s) 117
BanI GGYRCC 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 833
Bbv12I GWGCWC 1 cut(s) 1621
BbvCI CCTCAGC 2 cut(s) 485, 1638
BbvI GCAGC 5 cut(s) 514, 1000, 1162, 1450, 1558
BccI CCATC 7 cut(s) 104, 637, 970, 1025, 1409, 1486, 1556
BceAI ACGGC 2 cut(s) 30, 1236
BciT130I CCWGG 1 cut(s) 1117
BclI TGATCA 2 cut(s) 385, 1294
BcnI CCSGG 5 cut(s) 223, 767, 1368, 1412, 1644
BcoDI GTCTC 1 cut(s) 1211
BfoI RGCGCY 2 cut(s) 1305, 1326
Bme1390I CCNGG 6 cut(s) 223, 767, 1117, 1368, 1412, 1644
Bme18I GGWCC 2 cut(s) 79, 1444
BmgT120I GGNCC 3 cut(s) 79, 1113, 1444
BmiI GGNNCC 4 cut(s) 116, 1089, 1114, 1446
BmrFI CCNGG 6 cut(s) 223, 767, 1117, 1368, 1412, 1644
BmrI ACTGGG 1 cut(s) 1207
BmsI GCATC 4 cut(s) 664, 1000, 1557, 1623
BmuI ACTGGG 1 cut(s) 1207
BpiI GAAGAC 1 cut(s) 833
Bpu10I CCTNAGC 4 cut(s) 485, 663, 978, 1638
BpuEI CTTGAG 3 cut(s) 59, 272, 827
BpuMI CCSGG 5 cut(s) 223, 767, 1368, 1412, 1644
BsaAI YACGTR 1 cut(s) 1486
BsaJI CCNNGG 3 cut(s) 1116, 1367, 1410
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 446, 476, 934, 964, 1336, 1366
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 1136, 1373, 1416, 1432, 1643
Bse118I RCCGGY 2 cut(s) 433, 517
Bse1I ACTGG 2 cut(s) 1202, 1391
Bse3DI GCAATG 1 cut(s) 1368
BseBI CCWGG 1 cut(s) 1117
BseDI CCNNGG 3 cut(s) 1116, 1367, 1410
BseGI GGATG 4 cut(s) 904, 1033, 1420, 1572
BseLI CCNNNNNNNGG 6 cut(s) 60, 1136, 1373, 1416, 1432, 1643
BseMI GCAATG 1 cut(s) 1368
BseMII CTCAG 6 cut(s) 476, 630, 750, 840, 1170, 1652
BseNI ACTGG 2 cut(s) 1202, 1391
BseRI GAGGAG 7 cut(s) 146, 269, 878, 899, 942, 1464, 1611
BseSI GKGCMC 1 cut(s) 117
BseX3I CGGCCG 1 cut(s) 449
BseXI GCAGC 5 cut(s) 514, 1000, 1162, 1450, 1558
BsgI GTGCAG 2 cut(s) 794, 1508
Bsh1285I CGRYCG 1 cut(s) 452
BshFI GGCC 5 cut(s) 451, 517, 838, 976, 1115
BshNI GGYRCC 1 cut(s) 114
BshTI ACCGGT 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 1621
BsiSI CCGG 9 cut(s) 222, 434, 452, 518, 767, 973, 1367, 1412, 1643
BslFI GGGAC 1 cut(s) 1432
BslI CCNNNNNNNGG 6 cut(s) 60, 1136, 1373, 1416, 1432, 1643
BsmAI GTCTC 1 cut(s) 1211
BsmBI CGTCTC 1 cut(s) 1211
BsmFI GGGAC 1 cut(s) 1432
BsmI GAATGC 2 cut(s) 199, 922
BsnI GGCC 5 cut(s) 451, 517, 838, 976, 1115
Bsp1286I GDGCHC 2 cut(s) 117, 1621
Bsp143I GATC 5 cut(s) 214, 319, 385, 549, 1294
BspANI GGCC 5 cut(s) 451, 517, 838, 976, 1115
BspCNI CTCAG 6 cut(s) 477, 631, 751, 841, 1171, 1651
BspLI GGNNCC 4 cut(s) 116, 1089, 1114, 1446
BspPI GGATC 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 927
BspT107I GGYRCC 1 cut(s) 114
BsrDI GCAATG 1 cut(s) 1368
BsrFI RCCGGY 2 cut(s) 433, 517
BsrI ACTGG 2 cut(s) 1202, 1391
BssAI RCCGGY 2 cut(s) 433, 517
BssECI CCNNGG 3 cut(s) 1116, 1367, 1410
BssMI GATC 5 cut(s) 214, 319, 385, 549, 1294
Bst2UI CCWGG 1 cut(s) 1117
Bst4CI ACNGT 2 cut(s) 31, 1423
Bst6I CTCTTC 4 cut(s) 401, 406, 927, 1552
BstAPI GCANNNNNTGC 1 cut(s) 1519
BstBAI YACGTR 1 cut(s) 1486
BstC8I GCNNGC 4 cut(s) 515, 1375, 1398, 1407
BstF5I GGATG 4 cut(s) 904, 1033, 1420, 1572
BstH2I RGCGCY 2 cut(s) 1305, 1326
BstHHI GCGC 6 cut(s) 199, 559, 662, 1304, 1325, 1663
BstKTI GATC 5 cut(s) 217, 322, 388, 552, 1297
BstMAI GTCTC 1 cut(s) 1211
BstMBI GATC 5 cut(s) 214, 319, 385, 549, 1294
BstMCI CGRYCG 1 cut(s) 452
BstNI CCWGG 1 cut(s) 1117
BstNSI RCATGY 1 cut(s) 1291
BstSCI CCNGG 6 cut(s) 221, 765, 1115, 1366, 1410, 1642
BstSLI GKGCMC 1 cut(s) 117
BstV1I GCAGC 5 cut(s) 514, 1000, 1162, 1450, 1558
BstV2I GAAGAC 1 cut(s) 833
BstZI CGGCCG 1 cut(s) 449
BsuRI GGCC 5 cut(s) 451, 517, 838, 976, 1115
BtsCI GGATG 4 cut(s) 904, 1033, 1420, 1572
BtsI GCAGTG 3 cut(s) 942, 1437, 1621
BtsIMutI CAGTG 4 cut(s) 942, 1398, 1437, 1621
Cac8I GCNNGC 4 cut(s) 515, 1375, 1398, 1407
CaiI CAGNNNCTG 1 cut(s) 1178
CfoI GCGC 6 cut(s) 199, 559, 662, 1304, 1325, 1663
Cfr10I RCCGGY 2 cut(s) 433, 517
Cfr13I GGNCC 3 cut(s) 79, 1113, 1444
Csp6I GTAC 3 cut(s) 367, 429, 1651
CspAI ACCGGT 1 cut(s) 433
CviAII CATG 5 cut(s) 218, 244, 1288, 1316, 1501
CviQI GTAC 3 cut(s) 367, 429, 1651
DpnI GATC 5 cut(s) 216, 321, 387, 551, 1296
DpnII GATC 5 cut(s) 214, 319, 385, 549, 1294
DraIII CACNNNGTG 2 cut(s) 1318, 1432
EaeI YGGCCR 1 cut(s) 449
EagI CGGCCG 1 cut(s) 449
Eam1104I CTCTTC 4 cut(s) 401, 406, 927, 1552
EarI CTCTTC 4 cut(s) 401, 406, 927, 1552
EciI GGCGGA 1 cut(s) 918
EclXI CGGCCG 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 79, 1444
Eco52I CGGCCG 1 cut(s) 449
EcoO109I RGGNCCY 3 cut(s) 79, 1113, 1444
EcoRII CCWGG 1 cut(s) 1115
Esp3I CGTCTC 1 cut(s) 1211
FaeI CATG 5 cut(s) 221, 247, 1291, 1319, 1504
FaqI GGGAC 1 cut(s) 1432
FatI CATG 5 cut(s) 217, 243, 1287, 1315, 1500
FbaI TGATCA 2 cut(s) 385, 1294
FokI GGATG 4 cut(s) 911, 1040, 1427, 1579
GlaI GCGC 6 cut(s) 198, 558, 661, 1303, 1324, 1662
HaeII RGCGCY 2 cut(s) 1305, 1326
HaeIII GGCC 5 cut(s) 451, 517, 838, 976, 1115
HapII CCGG 9 cut(s) 222, 434, 452, 518, 767, 973, 1367, 1412, 1643
HhaI GCGC 6 cut(s) 199, 559, 662, 1304, 1325, 1663
Hin1II CATG 5 cut(s) 221, 247, 1291, 1319, 1504
Hin6I GCGC 6 cut(s) 197, 557, 660, 1302, 1323, 1661
HinP1I GCGC 6 cut(s) 197, 557, 660, 1302, 1323, 1661
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 5 cut(s) 315, 340, 844, 1035, 1268
HpaII CCGG 9 cut(s) 222, 434, 452, 518, 767, 973, 1367, 1412, 1643
HphI GGTGA 5 cut(s) 448, 478, 559, 925, 1027
Hpy166II GTNNAC 3 cut(s) 431, 636, 1426
Hpy188I TCNGA 7 cut(s) 153, 171, 381, 501, 760, 876, 967
Hpy188III TCNNGA 7 cut(s) 188, 251, 291, 323, 344, 817, 848
Hpy8I GTNNAC 3 cut(s) 431, 636, 1426
HpyAV CCTTC 6 cut(s) 129, 253, 568, 814, 1140, 1237
HpyCH4III ACNGT 2 cut(s) 31, 1423
HpyCH4IV ACGT 3 cut(s) 9, 751, 1485
HpyCH4V TGCA 8 cut(s) 146, 513, 530, 749, 775, 988, 1489, 1570
HpySE526I ACGT 3 cut(s) 9, 751, 1485
Hsp92II CATG 5 cut(s) 221, 247, 1291, 1319, 1504
HspAI GCGC 6 cut(s) 197, 557, 660, 1302, 1323, 1661
Ksp22I TGATCA 2 cut(s) 385, 1294
Kzo9I GATC 5 cut(s) 214, 319, 385, 549, 1294
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 2 cut(s) 1093, 1624
Lsp1109I GCAGC 5 cut(s) 514, 1000, 1162, 1450, 1558
LweI GCATC 4 cut(s) 664, 1000, 1557, 1623
MaeII ACGT 3 cut(s) 9, 751, 1485
MaeIII GTNAC 3 cut(s) 10, 188, 1000
MalI GATC 5 cut(s) 216, 321, 387, 551, 1296
MboI GATC 5 cut(s) 214, 319, 385, 549, 1294
MboII GAAGA 5 cut(s) 418, 423, 838, 914, 1569
MfeI CAATTG 1 cut(s) 1331
MhlI GDGCHC 2 cut(s) 117, 1621
MluCI AATT 9 cut(s) 172, 359, 373, 744, 904, 1018, 1061, 1202, 1331
MlyI GAGTC 1 cut(s) 853
MmeI TCCRAC 1 cut(s) 131
MseI TTAA 1 cut(s) 495
MspA1I CMGCKG 2 cut(s) 1043, 1175
MspI CCGG 9 cut(s) 222, 434, 452, 518, 767, 973, 1367, 1412, 1643
MspR9I CCNGG 6 cut(s) 223, 767, 1117, 1368, 1412, 1644
MunI CAATTG 1 cut(s) 1331
Mva1269I GAATGC 2 cut(s) 199, 922
MvaI CCWGG 1 cut(s) 1117
NciI CCSGG 5 cut(s) 223, 767, 1368, 1412, 1644
NdeII GATC 5 cut(s) 214, 319, 385, 549, 1294
NlaIII CATG 5 cut(s) 221, 247, 1291, 1319, 1504
NlaIV GGNNCC 4 cut(s) 116, 1089, 1114, 1446
NmuCI GTSAC 3 cut(s) 10, 188, 1000
NspI RCATGY 1 cut(s) 1291
PciSI GCTCTTC 1 cut(s) 927
PctI GAATGC 2 cut(s) 199, 922
PfeI GAWTC 4 cut(s) 315, 340, 1035, 1268
PflFI GACNNNGTC 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 1432
PfoI TCCNGGA 1 cut(s) 765
PinAI ACCGGT 1 cut(s) 433
PleI GAGTC 1 cut(s) 852
PpsI GAGTC 1 cut(s) 852
Ppu21I YACGTR 1 cut(s) 1486
PpuMI RGGWCCY 2 cut(s) 79, 1444
Psp5II RGGWCCY 2 cut(s) 79, 1444
Psp6I CCWGG 1 cut(s) 1115
PspGI CCWGG 1 cut(s) 1115
PspN4I GGNNCC 4 cut(s) 116, 1089, 1114, 1446
PspPI GGNCC 3 cut(s) 79, 1113, 1444
PspPPI RGGWCCY 2 cut(s) 79, 1444
PstNI CAGNNNCTG 1 cut(s) 1178
PsyI GACNNNGTC 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 1043, 1175
RsaI GTAC 3 cut(s) 368, 430, 1652
RsaNI GTAC 3 cut(s) 367, 429, 1651
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 1 cut(s) 495
Sau3AI GATC 5 cut(s) 214, 319, 385, 549, 1294
Sau96I GGNCC 3 cut(s) 79, 1113, 1444
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 6 cut(s) 223, 767, 1117, 1368, 1412, 1644
SduI GDGCHC 2 cut(s) 117, 1621
SfaNI GCATC 4 cut(s) 664, 1000, 1557, 1623
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 2 cut(s) 79, 1444
SmlI CTYRAG 3 cut(s) 74, 251, 806
SmoI CTYRAG 3 cut(s) 74, 251, 806
Sse9I AATT 9 cut(s) 172, 359, 373, 744, 904, 1018, 1061, 1202, 1331
StyD4I CCNGG 6 cut(s) 221, 765, 1115, 1366, 1410, 1642
TaaI ACNGT 2 cut(s) 31, 1423
TaiI ACGT 3 cut(s) 12, 754, 1488
TaqI TCGA 5 cut(s) 35, 290, 318, 343, 672
TasI AATT 9 cut(s) 172, 359, 373, 744, 904, 1018, 1061, 1202, 1331
TatI WGTACW 1 cut(s) 428
TauI GCSGC 5 cut(s) 20, 451, 1088, 1457, 1605
TfiI GAWTC 4 cut(s) 315, 340, 1035, 1268
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 4 cut(s) 949, 1398, 1437, 1621
TseFI GTSAC 3 cut(s) 10, 188, 1000
TseI GCWGC 5 cut(s) 527, 988, 1175, 1438, 1546
Tsp45I GTSAC 3 cut(s) 10, 188, 1000
TspDTI ATGAA 3 cut(s) 17, 83, 1260
TspGWI ACGGA 1 cut(s) 1467
TspRI CASTG 4 cut(s) 949, 1398, 1437, 1621
Tth111I GACNNNGTC 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 1432
VpaK11BI GGWCC 2 cut(s) 79, 1444
XapI RAATTY 4 cut(s) 172, 359, 1018, 1061
XceI RCATGY 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.