pycom02g03130

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015515
Physical Location & Seq
Reverse (-)
2039055 .. 2040497
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGTCCAGAACTCACAATCCACGCCAATGGCTTCCAGTCCTGCTCAATCTCCTCTTCCTCCTACAGCTTTTCGACACATCAAACGCCGACGTCGGCACTGCTGCCCAGTACCCTCCTCCATATTTACCGACGGAATGTTTTGGAGGCGATTCGTCGCAGTTTCCCTCGAGTAATCTATTCGCAGCGGCCGGTGACGGAATATGGGACAATGGAGCGTCGTGTGGGAGGCAGTACTTGGTGAGATGCATAAGTGCAGAAGAAACGGGGACGTGTGTTCCGGACAAGACGATTCAAGTGAAGATTGTGGACTATGCCGGAAGTATTAACAAAGCATCGGTTTCCGGCACCACCATGGTCTTGTCCCAAACAGCCTTTGGATCTGTTGCTAATTCGACGGCTAGATCCATTAACATTGAGTTTCAACAGTTAGTCCATCCATTTCTCTTCACCTCAGTTCCTCTTTATATTCTGTTTTGTTTTGATAGGTATGATATGGAAGATGCAGAAATGATCAAGAGTGGGTTTGAATTTTCAATCATATTCTTCTGCAGGAGTGGAACGTGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

190

Amino Acids

20.73

Weight (kDa)

4.74

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 66 - 139 9.8e-09 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015515)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03970 FvH4_1g03970 FvH4_1g03970
malus_domestica MD02G1038100.v1.1
prunus_persica Prupe.7G237200_v2.0.a1
pyrus_communis pycom02g03130 pycom15g16020
rosa_chinensis RchiOBHm_Chr2g0089451
rosa_laevigata RLG00000016030
rosa_multiflora Rmu_sc0000197.1_g000007
rosa_roxburghii Rroxscaffold_2G00151920
rosa_rugosa Rorug02G0001000
rosa_samantha Rh2BG044700 Rh2CG046500 Rh2DG046100
rosa_wichuraiana Rw2G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 1 cut(s) 185
AclWI GGATC 2 cut(s) 385, 396
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 2 cut(s) 110, 233
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 4 cut(s) 293, 422, 527, 534
AjiI CACGTC 1 cut(s) 270
AloI GAACNNNNNNTCC 1 cut(s) 33
AluBI AGCT 2 cut(s) 67, 567
AluI AGCT 2 cut(s) 67, 567
AlwI GGATC 2 cut(s) 385, 396
Ama87I CYCGRG 1 cut(s) 166
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 3 cut(s) 101, 182, 564
ApoI RAATTY 1 cut(s) 527
AsuHPI GGTGA 3 cut(s) 203, 250, 439
AvaI CYCGRG 1 cut(s) 166
BanI GGYRCC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 88, 194
BccI CCATC 1 cut(s) 441
BceAI ACGGC 1 cut(s) 411
BclI TGATCA 1 cut(s) 510
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 2 cut(s) 62, 547
BisI GCNGC 4 cut(s) 102, 183, 186, 565
BlsI GCNGC 4 cut(s) 103, 184, 187, 566
BmcAI AGTACT 1 cut(s) 233
BmeT110I CYCGRG 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 346
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 233, 341, 490
BmuI ACTGGG 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 277
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 35, 106
BseAI TCCGGA 1 cut(s) 277
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 35, 106
BseRI GAGGAG 2 cut(s) 41, 105
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 2 cut(s) 88, 194
BsgI GTGCAG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 188
BshNI GGYRCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 4 cut(s) 189, 278, 315, 342
BslFI GGGAC 3 cut(s) 218, 280, 346
BsmFI GGGAC 3 cut(s) 218, 280, 346
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 3 cut(s) 377, 401, 510
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 1 cut(s) 464
BspEI TCCGGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 551
BspPI GGATC 2 cut(s) 385, 396
BspT107I GGYRCC 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 2 cut(s) 35, 106
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 3 cut(s) 377, 401, 510
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 426
Bst6I CTCTTC 2 cut(s) 59, 449
BstACI GRCGYC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 451
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 433
BstKTI GATC 3 cut(s) 380, 404, 513
BstMBI GATC 3 cut(s) 377, 401, 510
BstMCI CGRYCG 1 cut(s) 189
BstSFI CTRYAG 2 cut(s) 62, 547
BstV1I GCAGC 2 cut(s) 88, 194
BstX2I RGATCY 2 cut(s) 377, 401
BstXI CCANNNNNNTGG 1 cut(s) 27
BstYI RGATCY 2 cut(s) 377, 401
BstZI CGGCCG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 433
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cfr10I RCCGGY 1 cut(s) 188
CseI GACGC 1 cut(s) 204
Csp6I GTAC 2 cut(s) 109, 232
CviAII CATG 1 cut(s) 352
CviJI RGCY 6 cut(s) 31, 67, 188, 371, 398, 567
CviKI_1 RGCY 6 cut(s) 31, 67, 188, 371, 398, 567
CviQI GTAC 2 cut(s) 109, 232
DdeI CTNAG 1 cut(s) 451
DpnI GATC 3 cut(s) 379, 403, 512
DpnII GATC 3 cut(s) 377, 401, 510
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 59, 449
EarI CTCTTC 2 cut(s) 59, 449
EclXI CGGCCG 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 351
Eco52I CGGCCG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 1 cut(s) 355
FaiI YATR 9 cut(s) 121, 202, 248, 312, 353, 465, 489, 494, 539
FaqI GGGAC 3 cut(s) 218, 280, 346
FatI CATG 1 cut(s) 351
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 4 cut(s) 102, 183, 186, 565
FokI GGATG 1 cut(s) 420
Fsp4HI GCNGC 4 cut(s) 102, 183, 186, 565
FspBI CTAG 1 cut(s) 399
GluI GCNGC 4 cut(s) 102, 183, 186, 565
HaeIII GGCC 1 cut(s) 188
HapII CCGG 4 cut(s) 189, 278, 315, 342
HgaI GACGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 1 cut(s) 355
HinfI GANTC 2 cut(s) 149, 289
HpaII CCGG 4 cut(s) 189, 278, 315, 342
HphI GGTGA 3 cut(s) 203, 250, 439
Hpy166II GTNNAC 1 cut(s) 307
Hpy188III TCNNGA 3 cut(s) 6, 278, 514
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 6 cut(s) 92, 95, 133, 157, 220, 397
HpyCH4III ACNGT 1 cut(s) 426
HpyCH4IV ACGT 3 cut(s) 90, 269, 560
HpyCH4V TGCA 5 cut(s) 246, 254, 503, 549, 564
HpyF3I CTNAG 1 cut(s) 451
HpySE526I ACGT 3 cut(s) 90, 269, 560
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 1 cut(s) 355
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 3 cut(s) 377, 401, 510
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 9 cut(s) 19, 48, 53, 119, 202, 291, 328, 355, 535
Lsp1109I GCAGC 2 cut(s) 88, 194
LweI GCATC 3 cut(s) 233, 341, 490
MaeI CTAG 1 cut(s) 399
MaeII ACGT 3 cut(s) 90, 269, 560
MaeIII GTNAC 1 cut(s) 191
MalI GATC 3 cut(s) 379, 403, 512
MboI GATC 3 cut(s) 377, 401, 510
MboII GAAGA 6 cut(s) 46, 269, 310, 436, 509, 535
MflI RGATCY 2 cut(s) 377, 401
MluCI AATT 2 cut(s) 388, 527
MnlI CCTC 9 cut(s) 62, 68, 123, 126, 137, 175, 219, 460, 468
Mph1103I ATGCAT 1 cut(s) 248
MroI TCCGGA 1 cut(s) 277
MseI TTAA 2 cut(s) 324, 408
MslI CAYNNNNRTG 2 cut(s) 25, 350
MspA1I CMGCKG 2 cut(s) 185, 567
MspI CCGG 4 cut(s) 189, 278, 315, 342
NcoI CCATGG 1 cut(s) 351
NdeII GATC 3 cut(s) 377, 401, 510
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 248
PaeR7I CTCGAG 1 cut(s) 166
PfeI GAWTC 2 cut(s) 149, 289
PkrI GCNGC 4 cut(s) 103, 184, 187, 566
PspN4I GGNNCC 1 cut(s) 346
PspXI VCTCGAGB 1 cut(s) 166
PstI CTGCAG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 377, 401
PvuII CAGCTG 1 cut(s) 567
RsaI GTAC 2 cut(s) 110, 233
RsaNI GTAC 2 cut(s) 109, 232
RseI CAYNNNNRTG 2 cut(s) 25, 350
SaqAI TTAA 2 cut(s) 324, 408
SatI GCNGC 4 cut(s) 102, 183, 186, 565
Sau3AI GATC 3 cut(s) 377, 401, 510
ScaI AGTACT 1 cut(s) 233
SetI ASST 7 cut(s) 69, 93, 272, 452, 488, 563, 569
SfaNI GCATC 3 cut(s) 233, 341, 490
SfcI CTRYAG 2 cut(s) 62, 547
Sfr274I CTCGAG 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 25, 350
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 2 cut(s) 388, 527
SsiI CCGC 1 cut(s) 185
SspMI CTAG 1 cut(s) 399
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 426
TaiI ACGT 3 cut(s) 93, 272, 563
TaqI TCGA 3 cut(s) 72, 167, 392
TasI AATT 2 cut(s) 388, 527
TatI WGTACW 1 cut(s) 231
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 149, 289
Tru1I TTAA 2 cut(s) 324, 408
Tru9I TTAA 2 cut(s) 324, 408
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 101, 182, 564
Tsp45I GTSAC 1 cut(s) 191
TspGWI ACGGA 2 cut(s) 146, 210
TspRI CASTG 1 cut(s) 103
XapI RAATTY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 166
XspI CTAG 1 cut(s) 399
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.