Rw2G003650

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015515
Physical Location & Seq
Forward (+)
3350510 .. 3352277
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003650.1

Sequence Viewer

Length: 435 bp
ATGACCAGAGCACATCCACAAAATTTGCGATTCACTTTCTTATTCATCATCCTCTTGTCTACACAGCATTTCCTAACTTCAAACGGCGACGTTGGCACTGCTGCTCAGTATTCTCCCCCATATCTACCCACGGACTGTTATGGAAGCGACTCGTCGCAGTTTCCGTCAAGTAATTTGTTTGCGGCGGCCGGAGATGGAATATGGGACAATGGAGCTTCGTGTGGGAGGCAGTACTTGGTAAGATGCATTAGCGCGGCCGTAACGGGGACGTGTATTCCGGACACGACGATTCAGATAAGAATTGTGGACTATGCTGCCGGCGTTGCTTCGAAGCCGTCGGCGGATGGCACCACCATGGTGTTGTCTCAGACGGCTTTCGCTGCTATTGCCAACTCAACGGCTACTTCCATCAACATTGAATTTCAACAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

144

Amino Acids

15.25

Weight (kDa)

4.95

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 66 - 141 4e-09 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015515)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03970 FvH4_1g03970 FvH4_1g03970
malus_domestica MD02G1038100.v1.1
prunus_persica Prupe.7G237200_v2.0.a1
pyrus_communis pycom02g03130 pycom15g16020
rosa_chinensis RchiOBHm_Chr2g0089451
rosa_laevigata RLG00000016030
rosa_multiflora Rmu_sc0000197.1_g000007
rosa_roxburghii Rroxscaffold_2G00151920
rosa_rugosa Rorug02G0001000
rosa_samantha Rh2BG044700 Rh2CG046500 Rh2DG046100
rosa_wichuraiana Rw2G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 4 cut(s) 182, 185, 254, 341
AcoI YGGCCR 2 cut(s) 186, 255
AcsI RAATTY 2 cut(s) 22, 419
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 264
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 3 cut(s) 81, 419, 425
AjiI CACGTC 1 cut(s) 270
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 1 cut(s) 369
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 2 cut(s) 186, 255
ApeKI GCWGC 3 cut(s) 101, 314, 380
ApoI RAATTY 2 cut(s) 22, 419
AspLEI GCGC 1 cut(s) 254
AsuII TTCGAA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 3 cut(s) 88, 301, 367
BccI CCATC 3 cut(s) 188, 338, 416
BceAI ACGGC 5 cut(s) 100, 242, 319, 387, 414
BcoDI GTCTC 1 cut(s) 369
BisI GCNGC 6 cut(s) 102, 183, 186, 255, 315, 381
BlsI GCNGC 6 cut(s) 103, 184, 187, 256, 316, 382
BmcAI AGTACT 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 349
BmsI GCATC 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 329
BsaJI CCNNGG 2 cut(s) 129, 354
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 277
BseDI CCNNGG 2 cut(s) 129, 354
BseGI GGATG 3 cut(s) 13, 48, 349
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 119, 380
BseX3I CGGCCG 2 cut(s) 186, 255
BseXI GCAGC 3 cut(s) 88, 301, 367
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 2 cut(s) 189, 258
BshFI GGCC 2 cut(s) 188, 257
BshNI GGYRCC 1 cut(s) 347
BsiEI CGRYCG 2 cut(s) 189, 258
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 3 cut(s) 189, 278, 318
BslFI GGGAC 2 cut(s) 218, 280
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 369
BsmFI GGGAC 2 cut(s) 218, 280
BsnI GGCC 2 cut(s) 188, 257
Bsp119I TTCGAA 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 13
Bsp13I TCCGGA 1 cut(s) 277
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 4 cut(s) 182, 185, 254, 341
BspANI GGCC 2 cut(s) 188, 257
BspCNI CTCAG 2 cut(s) 118, 379
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 129, 354
BssT1I CCWWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 2 cut(s) 105, 366
BstDSI CCRYGG 2 cut(s) 129, 354
BstF5I GGATG 3 cut(s) 13, 48, 349
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 254
BstMAI GTCTC 1 cut(s) 369
BstMCI CGRYCG 2 cut(s) 189, 258
BstMWI GCNNNNNNNGC 4 cut(s) 93, 323, 380, 386
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 88, 301, 367
BstZI CGGCCG 2 cut(s) 186, 255
BsuRI GGCC 2 cut(s) 188, 257
BtgI CCRYGG 2 cut(s) 129, 354
BtrI CACGTC 1 cut(s) 270
BtsCI GGATG 3 cut(s) 13, 48, 349
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 319
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 317
Csp6I GTAC 1 cut(s) 232
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 1 cut(s) 355
CviJI RGCY 6 cut(s) 188, 215, 257, 334, 374, 401
CviKI_1 RGCY 6 cut(s) 188, 215, 257, 334, 374, 401
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 2 cut(s) 105, 366
EaeI YGGCCR 2 cut(s) 186, 255
EagI CGGCCG 2 cut(s) 186, 255
EciI GGCGGA 1 cut(s) 356
EclXI CGGCCG 2 cut(s) 186, 255
Eco130I CCWWGG 1 cut(s) 354
Eco52I CGGCCG 2 cut(s) 186, 255
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 358
FaiI YATR 6 cut(s) 121, 141, 202, 312, 356, 433
FaqI GGGAC 2 cut(s) 218, 280
FatI CATG 1 cut(s) 354
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 6 cut(s) 102, 183, 186, 255, 315, 381
FokI GGATG 2 cut(s) 35, 356
Fsp4HI GCNGC 6 cut(s) 102, 183, 186, 255, 315, 381
GlaI GCGC 1 cut(s) 253
GluI GCNGC 6 cut(s) 102, 183, 186, 255, 315, 381
HaeIII GGCC 2 cut(s) 188, 257
HapII CCGG 3 cut(s) 189, 278, 318
HhaI GCGC 1 cut(s) 254
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 3 cut(s) 30, 149, 289
HpaII CCGG 3 cut(s) 189, 278, 318
Hpy166II GTNNAC 2 cut(s) 60, 307
Hpy188I TCNGA 2 cut(s) 294, 369
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 2 cut(s) 60, 307
Hpy99I CGWCG 4 cut(s) 92, 157, 289, 340
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4IV ACGT 2 cut(s) 90, 269
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 323, 380, 386
HpyF3I CTNAG 2 cut(s) 105, 366
HpySE526I ACGT 2 cut(s) 90, 269
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 1 cut(s) 252
Kpn2I TCCGGA 1 cut(s) 277
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 5 cut(s) 19, 202, 291, 331, 413
Lsp1109I GCAGC 3 cut(s) 88, 301, 367
LweI GCATC 1 cut(s) 233
MaeII ACGT 2 cut(s) 90, 269
MaeIII GTNAC 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 4 cut(s) 22, 172, 300, 419
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 2 cut(s) 62, 219
Mph1103I ATGCAT 1 cut(s) 248
MroI TCCGGA 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 317
MslI CAYNNNNRTG 2 cut(s) 353, 356
MspI CCGG 3 cut(s) 189, 278, 318
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 4 cut(s) 93, 323, 380, 386
NaeI GCCGGC 1 cut(s) 319
NcoI CCATGG 1 cut(s) 354
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 1 cut(s) 349
NsiI ATGCAT 1 cut(s) 248
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 30, 289
PkrI GCNGC 6 cut(s) 103, 184, 187, 256, 316, 382
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 349
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 2 cut(s) 353, 356
SatI GCNGC 6 cut(s) 102, 183, 186, 255, 315, 381
ScaI AGTACT 1 cut(s) 233
SchI GAGTC 1 cut(s) 143
SduI GDGCHC 1 cut(s) 13
SetI ASST 4 cut(s) 93, 217, 272, 432
SfaNI GCATC 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 353, 356
Sse9I AATT 4 cut(s) 22, 172, 300, 419
SsiI CCGC 4 cut(s) 182, 185, 254, 341
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 137
TaiI ACGT 2 cut(s) 93, 272
TaqI TCGA 1 cut(s) 329
TasI AATT 4 cut(s) 22, 172, 300, 419
TatI WGTACW 1 cut(s) 231
TauI GCSGC 3 cut(s) 185, 188, 257
TfiI GAWTC 2 cut(s) 30, 289
TscAI CASTG 1 cut(s) 103
TseI GCWGC 3 cut(s) 101, 314, 380
TspDTI ATGAA 1 cut(s) 34
TspGWI ACGGA 2 cut(s) 146, 153
TspRI CASTG 1 cut(s) 103
XapI RAATTY 2 cut(s) 22, 419
XmiI GTMKAC 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.