Rroxscaffold_2G00151920

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015515
Physical Location & Seq
Forward (+)
89049612 .. 89051545
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151920.1

Sequence Viewer

Length: 516 bp
ATGTTATTATGGACATTTCCCTTAACGCAAGTATTGTCCCCTTTAGCCACGGACTGTTATGGAAGCGACTCGTCGCAGTTTCCGTCGAGTAATTTGTTTGCGGCGGCCGGTGATGGAATATGGGACAATGGAGCTTCGTGTGGGAGGCAGTACTTGGTGAGATGCATTAGCGCGGCCGTAACGGGGACGTGTATTCAGGACACGACGATTCAGATCAGAATTGTGGACTATGCTGCCGGCGTTGCTTCGAAGCCGTCTGCGGATGGCACCACCATGGTGTTGTCTCAGACGGCTTTCGCTGCTATTGCCAACTCCACGGCTGCTTCCATCAACATTGAATTTCAACAATCTGATCAGTACGTGGTTGCAGTCTTGGAAATATCTAGGAAGGTTAATAATCAGATAAGTCTGTTATATAGAGCTCAAGCTTTTTATGAGCTTATTTCCAGGCTTGAAAATAATGAACCACATGAATGTAATCGATCCAAATTAAGAAGGCTCCATCATCATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

171

Amino Acids

18.71

Weight (kDa)

5.74

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 39 - 113 1.6e-08 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015515)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03970 FvH4_1g03970 FvH4_1g03970
malus_domestica MD02G1038100.v1.1
prunus_persica Prupe.7G237200_v2.0.a1
pyrus_communis pycom02g03130 pycom15g16020
rosa_chinensis RchiOBHm_Chr2g0089451
rosa_laevigata RLG00000016030
rosa_multiflora Rmu_sc0000197.1_g000007
rosa_roxburghii Rroxscaffold_2G00151920
rosa_rugosa Rorug02G0001000
rosa_samantha Rh2BG044700 Rh2CG046500 Rh2DG046100
rosa_wichuraiana Rw2G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccII CGCG 1 cut(s) 173
AciI CCGC 4 cut(s) 101, 104, 173, 260
AclWI GGATC 1 cut(s) 477
AcoI YGGCCR 2 cut(s) 105, 174
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 2 cut(s) 152, 359
AfiI CCNNNNNNNGG 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 188
AgsI TTSAA 3 cut(s) 338, 344, 455
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 446
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 4 cut(s) 134, 422, 428, 439
AluI AGCT 4 cut(s) 134, 422, 428, 439
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 477
AoxI GGCC 2 cut(s) 105, 174
ApeKI GCWGC 3 cut(s) 233, 299, 320
ApoI RAATTY 1 cut(s) 338
AspLEI GCGC 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 122, 169
AsuII TTCGAA 1 cut(s) 248
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 3 cut(s) 220, 286, 307
BccI CCATC 4 cut(s) 107, 257, 335, 510
BceAI ACGGC 4 cut(s) 161, 238, 306, 333
BciT130I CCWGG 1 cut(s) 448
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 384
BisI GCNGC 6 cut(s) 102, 105, 174, 234, 300, 321
BlsI GCNGC 6 cut(s) 103, 106, 175, 235, 301, 322
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 189
BmiI GGNNCC 2 cut(s) 268, 500
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 408
Bsa29I ATCGAT 1 cut(s) 481
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 212
BsaJI CCNNGG 3 cut(s) 48, 273, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 2 cut(s) 107, 236
Bse8I GATNNNNATC 1 cut(s) 212
BseBI CCWGG 1 cut(s) 448
BseCI ATCGAT 1 cut(s) 481
BseDI CCNNGG 3 cut(s) 48, 273, 315
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 299
BseX3I CGGCCG 2 cut(s) 105, 174
BseXI GCAGC 3 cut(s) 220, 286, 307
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 108, 177
BshFI GGCC 2 cut(s) 107, 176
BshNI GGYRCC 1 cut(s) 266
BshVI ATCGAT 1 cut(s) 481
BsiEI CGRYCG 2 cut(s) 108, 177
BsiHKAI GWGCWC 1 cut(s) 424
BsiSI CCGG 2 cut(s) 108, 237
BslFI GGGAC 3 cut(s) 22, 137, 199
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 22, 137, 199
BsnI GGCC 2 cut(s) 107, 176
Bsp119I TTCGAA 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 3 cut(s) 213, 352, 482
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 4 cut(s) 101, 104, 173, 260
BspANI GGCC 2 cut(s) 107, 176
BspCNI CTCAG 1 cut(s) 298
BspDI ATCGAT 1 cut(s) 481
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 268, 500
BspPI GGATC 1 cut(s) 477
BspT104I TTCGAA 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 107, 236
BssAI RCCGGY 2 cut(s) 107, 236
BssECI CCNNGG 3 cut(s) 48, 273, 315
BssMI GATC 3 cut(s) 213, 352, 482
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 3 cut(s) 48, 273, 315
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 216, 355, 485
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 3 cut(s) 213, 352, 482
BstMCI CGRYCG 2 cut(s) 108, 177
BstMWI GCNNNNNNNGC 3 cut(s) 242, 299, 305
BstNI CCWGG 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 446
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 220, 286, 307
BstZI CGGCCG 2 cut(s) 105, 174
Bsu15I ATCGAT 1 cut(s) 481
BsuRI GGCC 2 cut(s) 107, 176
BsuTUI ATCGAT 1 cut(s) 481
BtgI CCRYGG 3 cut(s) 48, 273, 315
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 238
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 2 cut(s) 107, 236
ClaI ATCGAT 1 cut(s) 481
Csp6I GTAC 2 cut(s) 151, 358
CviAII CATG 3 cut(s) 274, 470, 509
CviQI GTAC 2 cut(s) 151, 358
DdeI CTNAG 1 cut(s) 285
DpnI GATC 3 cut(s) 215, 354, 484
DpnII GATC 3 cut(s) 213, 352, 482
EaeI YGGCCR 2 cut(s) 105, 174
EagI CGGCCG 2 cut(s) 105, 174
Ecl136II GAGCTC 1 cut(s) 422
EclXI CGGCCG 2 cut(s) 105, 174
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 424
Eco52I CGGCCG 2 cut(s) 105, 174
Eco53kI GAGCTC 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 167
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 3 cut(s) 277, 473, 512
FaqI GGGAC 3 cut(s) 22, 137, 199
FatI CATG 3 cut(s) 273, 469, 508
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 6 cut(s) 102, 105, 174, 234, 300, 321
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 6 cut(s) 102, 105, 174, 234, 300, 321
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 172
GluI GCNGC 6 cut(s) 102, 105, 174, 234, 300, 321
HaeIII GGCC 2 cut(s) 107, 176
HapII CCGG 2 cut(s) 108, 237
HhaI GCGC 1 cut(s) 173
Hin1II CATG 3 cut(s) 277, 473, 512
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 2 cut(s) 68, 208
HpaII CCGG 2 cut(s) 108, 237
HphI GGTGA 2 cut(s) 122, 169
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 5 cut(s) 213, 218, 288, 352, 402
Hpy188III TCNNGA 2 cut(s) 197, 509
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 3 cut(s) 76, 88, 208
HpyAV CCTTC 2 cut(s) 382, 489
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 2 cut(s) 188, 360
HpyCH4V TGCA 2 cut(s) 165, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 299, 305
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 2 cut(s) 188, 360
Hsp92II CATG 3 cut(s) 277, 473, 512
HspAI GCGC 1 cut(s) 171
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 3 cut(s) 213, 352, 482
LmnI GCTCC 2 cut(s) 131, 504
LpnPI CCDG 5 cut(s) 121, 182, 250, 433, 460
Lsp1109I GCAGC 3 cut(s) 220, 286, 307
LweI GCATC 1 cut(s) 152
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 188, 360
MaeIII GTNAC 1 cut(s) 178
MalI GATC 3 cut(s) 215, 354, 484
MboI GATC 3 cut(s) 213, 352, 482
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 4 cut(s) 91, 219, 338, 488
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 236
MseI TTAA 3 cut(s) 23, 393, 491
MslI CAYNNNNRTG 4 cut(s) 272, 275, 472, 507
MspI CCGG 2 cut(s) 108, 237
MspR9I CCNGG 1 cut(s) 448
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 242, 299, 305
NaeI GCCGGC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 273
NdeII GATC 3 cut(s) 213, 352, 482
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 3 cut(s) 277, 473, 512
NlaIV GGNNCC 2 cut(s) 268, 500
NsiI ATGCAT 1 cut(s) 167
NspV TTCGAA 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 275
PagI TCATGA 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 6 cut(s) 103, 106, 175, 235, 301, 322
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 446
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 2 cut(s) 268, 500
RsaI GTAC 2 cut(s) 152, 359
RsaNI GTAC 2 cut(s) 151, 358
RseI CAYNNNNRTG 4 cut(s) 272, 275, 472, 507
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 3 cut(s) 23, 393, 491
SatI GCNGC 6 cut(s) 102, 105, 174, 234, 300, 321
Sau3AI GATC 3 cut(s) 213, 352, 482
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 1 cut(s) 424
SetI ASST 7 cut(s) 136, 191, 363, 393, 424, 430, 441
SfaNI GCATC 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 248
SmiMI CAYNNNNRTG 4 cut(s) 272, 275, 472, 507
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 4 cut(s) 91, 219, 338, 488
SsiI CCGC 4 cut(s) 101, 104, 173, 260
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 2 cut(s) 191, 363
TaqI TCGA 3 cut(s) 86, 248, 481
TasI AATT 4 cut(s) 91, 219, 338, 488
TatI WGTACW 1 cut(s) 150
TauI GCSGC 3 cut(s) 104, 107, 176
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 3 cut(s) 23, 393, 491
Tru9I TTAA 3 cut(s) 23, 393, 491
TseI GCWGC 3 cut(s) 233, 299, 320
TspDTI ATGAA 2 cut(s) 477, 486
TspGWI ACGGA 2 cut(s) 65, 72
XapI RAATTY 1 cut(s) 338
XspI CTAG 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.