pycom02g04660

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012242
Physical Location & Seq
Reverse (-)
3028427 .. 3032964
4538 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3054 bp
ATGTACTTTCGGTGGAAACATGAGAGGATGTATTCTCGTGGTTTCGGCTGTGTTAGCACTTGTAATTCTTATGCTAGCAGGACTCAACTTCTACTTTTTCAAGTCAATATGACGCCTGTAATTCCGTATTCTATAAGCAATGTCTCGCATATACCTGGAACTGCCTTTACTGCAAGAAAGAACAATTGTTTAACAAAATTCAGTTTTTCGAGGAAATCTGCAAGACATACTCTATCCCCACAGAGTTTTCTTTTACCTTTTTCGACCTCTATCAAATCTTTTCCATTGTACCACAGCAGGTGCCCCGTGCATCATAAATGTAGAATCCCTGTATCTGCTACAGGAACTGATGTAGCGGTTGAGGAAGCAGACTCACCAGTTGAAGATGCAGCTTCTGGTGAAGCCAAATCCCCTGAGGATAGTCCCAGCCCATCTCAAGATGCTCAACCAAAGCGTACAAAACCTGTTAGGAAAAGTGAGATGCCGCCTGTGAAGAATGAGGAGCTGGTTGTGGGTGCAACTTTTACCGGGAAAGTAAGATCAATCCAGCCATTTGGTGCTTTTATTGATTTTGGAGCTTTCACAGATGGACTCGTACATGTTTCACAATTGAGTGATAGTTACGTCAAGGACGTTGGAAGCATCGTTTCTGTTGGGCAAGAGGTGAAGGTGACATTAGTTGAAGCCAATCCGGAGACTGGGCGAATCTCTCTCACTATGCGTCAACGTGACGATGGCAGTAAGCCGCAGCAAAGGAAAGATGCTTCTGCTGGTAGTGATAGGGGTGGACCTGGTAGAAGGAGTGGCCCAAAGAAAGGCGATAGGAAAAATGAGGTGAGAAAAACTACGAAGTTTGAAAAGGGGCAGGACCTAGTGGGCACTGTAAAGAATTTTGGCAGGGCTGGTGCTTTTATATCTCTTCCTGAGGGGGAGGAAGGATTCCTCCCTACATCAGAGGAACCTGACGATGGATTTGCAAACGTCATGGGAGAGACCTCCCTGCAGCTTGGCCAAGAAATAAACGTCCGTGTCCTGCGTACTACAAGAGGACAAGTAACCTTGACAATGAAGAAAGAAGAAGATATTCTAAAGTCGGACTCGCAGGTCAGTCAGGGAGTAATCCACACTGCAACGAACCCATTCGTTCTGGCGTTCCGCCAAAACAAGGATATTGCTTCTTTTTTGGATGAAAGAGAGAAAATAGAAAAAGCAGCTAAAGCAATTCCAAGCAGTGAGTCCGGTATTCCTGAAGTGTTGGATGAAAAAGCAAGCAGCGAGCAGGGAACTCTTGGTGTCCCTGCAGCGGTAGATGAAACTGTTGAAAATGGTGGTGCTTCTTCAGAGGACCAGGAAAGCCCAGTTTCTAGTACAATTGAAACACTAGAAACTACAGAACAAACCATAGAAAAAGAAGAGGTGAGTTCTGATATCTTGGCGCCTGAAGGGAGTACATCTACCACGGATGGAGTGGAAAACGCAAGCGCAGATTTGTCTAGTGAAATAGCTAATCACACGTCGGCTTCAGAAATTCCAACAGGCGAAGAGGTTATAGAGCCTCAAGTGGATGATACCATAGCAAAAGTTGAACTGCAACCACCTACTTCAGAGAGTGAAATTCCTTCTGCTGCACTAACTGAAGAACCAAAAGAAAGTGAGGCCACCAAAGTAGTAGAAGACCTAGCTGACAACATTACGGAGGAAGCTCAGATTCAAACATCTGCTGCTGAGAGTGAATTGCCGTCTATCACACAAGTGGAAGATGACAAAGTGGACAGTTCTCCCGAGAAGAATGGCAGTGTTTCTAATTCAAATGGACAATCTGACAATCCTTCCCCGAAGGAAAGTAAACCTAAAGCTACTATATCACCAGCCCTTGTAAAGCAGCTGCGTGAAGAAACAGGAGCTGGAATGATGGATTGCAAAAATGCTTTGTCAGAGACTGGCGGGGACATTGTTAAAGCTACGGAGTTCCTCAGAAAGAAAGGTTTAGCAAGTGCAGAAAAGAAAGCCAGCAGAGCCACTGCTGAAGGAAGAATAGGTTCATACATTCACGATAGCAGAATAGGTATCTTGCTAGAGGTAAACTGTGAGACAGATTTTGTTTCTCGGGGTGACATTTTCAAGGAGCTGGTTGATGATTTAGCCATGCAAGTGGCTGCATGCCCTCAAGTACAGTACCTTGCTACAGAAGATGTTCCTGAAGAGTTTGTGACCAAGGAAAGAGCAATTGAGATGCAGAAAGAAGATCTTTTGTCAAAGCCAGAGCAGATTAGATCAAAGATTGTTGACGGGAGGATAAGGAAGAGGCTTGAGGAGCTGGCATTGCTTGAGCAGCCTTTCATTAAGAATGATAAGGTGGTGGTGAAGGACTTGGTCAAACAAACCATTTCAACTATTGGAGAAAACATAAAAGTGAAGAGGTTTGTGCGCTACAATCTTGGAGAGGGCTTGGAGAAGAAGAGTCAGGATTTTGCTGCTGAAGTGGCTGCCCAAACTGCGGCAAAACCAGTGGCCACGGAAGTAAAAGAGCAGCCTGCTGTAGTGGAAGTCAAAGAAACTGTTGAGAAGGCACCAACTGTAGCCGTCTCTGCTGCTTTGGTTAAACAACTACGTGATGAAACTGGAGCAGGGATGATGGACTGCAAGAAAGCTCTCTCCGAAACTGGAGGGGACCTTGAGAAGGCACAAGAGTACCTGAGGAAGAAGGGTCTTTCTTCTGCTGAAAAGAAATCCAGCAGGCTTGCTGCTGAAGGCAGGATTGGATCCTACATTCATGACGCCCGCATTGGAGTTCTGATTGAAGTAAACTGCGAGACTGACTTTGTTGGTCGAAGTGAAAATTTCAAGGGGTTGGTTGATGATCTAGCAATGCAAGTCGTGGCCTGCCCGCAGGTGCAATATGTATCCATCGAAGACATTCCAGAGAGCATGGTGAACAAGGAAAAAGAGCTTGAGAGGCAGAGGGAGGACCTTCTTTCAAAACCCGAGAACATTAGAGAGAGAATCGTGGAGGGGAGGATCGATCAAAGAGGCTTGGGGAGCTGGCTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF_TS S1

Protein Analysis

1018

Amino Acids

110.8

Weight (kDa)

5.05

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 171 - 241 3e-14 S1 RNA binding domain
S1_RRP5 PF23459 172 - 233 6.2e-06 RRP5 S1 domain
S1 PF00575 284 - 348 5.6e-06 S1 RNA binding domain
EF_TS PF00889 673 - 815 1.2e-28 Elongation factor TS
EF_TS PF00889 914 - 1009 1.7e-14 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29060 AT4G29060
fragaria_vesca FvH4_1g05650
malus_domestica MD02G1057500.v1.1 MD15G1191900.v1.1
prunus_persica Prupe.7G225300_v2.0.a1
pyrus_communis pycom02g04660
rosa_chinensis RchiOBHm_Chr2g0091281
rosa_laevigata RLG00000016202
rosa_multiflora Rmu_sc0002270.1_g000032
rosa_roxburghii Rroxscaffold_2G00150270
rosa_rugosa Rorug02G0015600 Rorug02G0015700 Rorug02G0015800
rosa_samantha Rh2AG061000 Rh2BG060000 Rh2CG062000 Rh2DG060500
rosa_wichuraiana Rw2G004880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 288, 2881
AasI GACNNNNNNGTC 1 cut(s) 1103
Acc36I ACCTGC 3 cut(s) 288, 1093, 2881
AccB1I GGYRCC 3 cut(s) 300, 1435, 2569
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 9 cut(s) 356, 485, 746, 1156, 1304, 1944, 2498, 2782, 2888
AclWI GGATC 3 cut(s) 2757, 2770, 3026
AcoI YGGCCR 2 cut(s) 1009, 2511
AcsI RAATTY 5 cut(s) 197, 889, 1527, 1614, 2839
AcyI GRCGYC 3 cut(s) 113, 1436, 2778
AfiI CCNNNNNNNGG 7 cut(s) 698, 815, 968, 1165, 1303, 2497, 2680
AflIII ACRYGT 2 cut(s) 598, 1512
AjiI CACGTC 1 cut(s) 1515
AjnI CCWGG 3 cut(s) 154, 790, 1347
AjuI GAANNNNNNNTTGG 2 cut(s) 2742, 2774
AleI CACNNNNGTG 1 cut(s) 1751
Alw26I GTCTC 7 cut(s) 148, 689, 986, 1931, 2084, 2590, 2807
AlwI GGATC 3 cut(s) 2757, 2770, 3026
AlwNI CAGNNNCTG 4 cut(s) 347, 395, 1904, 1940
Ama87I CYCGRG 3 cut(s) 1781, 2106, 2984
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 5 cut(s) 805, 1009, 1656, 2511, 2880
ApoI RAATTY 5 cut(s) 197, 889, 1527, 1614, 2839
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
Asp700I GAANNNNTTC 5 cut(s) 1083, 1139, 2038, 2193, 2916
AspLEI GCGC 3 cut(s) 1438, 1484, 2430
AspS9I GGNCC 6 cut(s) 788, 806, 868, 1345, 2671, 2968
AsuC2I CCSGG 1 cut(s) 529
AsuNHI GCTAGC 1 cut(s) 74
AvaI CYCGRG 3 cut(s) 1781, 2106, 2984
AvaII GGWCC 5 cut(s) 788, 868, 1345, 2671, 2968
AxyI CCTNAGG 3 cut(s) 414, 924, 2696
BaeGI GKGCMC 2 cut(s) 305, 881
BaeI ACNNNNGTAYC 2 cut(s) 2675, 2708
BalI TGGCCA 2 cut(s) 1011, 2513
BamHI GGATCC 1 cut(s) 2762
BanI GGYRCC 3 cut(s) 300, 1435, 2569
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 2 cut(s) 1680, 2919
BccI CCATC 8 cut(s) 439, 581, 728, 962, 1457, 1906, 2629, 2915
BceAI ACGGC 2 cut(s) 1723, 2567
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 3 cut(s) 156, 792, 1349
BciVI GTATCC 1 cut(s) 2914
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 7 cut(s) 148, 689, 986, 1931, 2084, 2590, 2807
BfaI CTAG 8 cut(s) 75, 872, 1365, 1382, 1494, 1679, 2075, 2864
BfmI CTRYAG 7 cut(s) 339, 1001, 1299, 1389, 2184, 2538, 2577
BfoI RGCGCY 1 cut(s) 1439
BfuAI ACCTGC 3 cut(s) 288, 1093, 2881
BfuI GTATCC 1 cut(s) 2914
BglII AGATCT 1 cut(s) 2245
Bme1390I CCNGG 4 cut(s) 156, 529, 792, 1349
Bme18I GGWCC 5 cut(s) 788, 868, 1345, 2671, 2968
BmeT110I CYCGRG 3 cut(s) 1781, 2106, 2984
BmgBI CACGTC 1 cut(s) 1515
BmgT120I GGNCC 6 cut(s) 788, 806, 868, 1345, 2671, 2968
BmiI GGNNCC 6 cut(s) 302, 960, 1437, 2571, 2672, 2764
BmrFI CCNGG 4 cut(s) 156, 529, 792, 1349
BmrI ACTGGG 2 cut(s) 708, 1352
BmsI GCATC 7 cut(s) 319, 376, 430, 471, 651, 751, 2223
BmtI GCTAGC 1 cut(s) 78
BmuI ACTGGG 2 cut(s) 708, 1352
BpiI GAAGAC 2 cut(s) 1680, 2919
BpmI CTGGAG 2 cut(s) 2643, 2685
BpuEI CTTGAG 7 cut(s) 420, 1542, 2151, 2330, 2348, 2696, 2972
BpuMI CCSGG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 3021
BsaAI YACGTR 1 cut(s) 2612
BsaHI GRCGYC 3 cut(s) 113, 1436, 2778
BsaI GGTCTC 1 cut(s) 986
BsaJI CCNNGG 3 cut(s) 1458, 2214, 2514
BsaWI WCCGGW 2 cut(s) 691, 1238
Bsc4I CCNNNNNNNGG 7 cut(s) 698, 815, 968, 1165, 1303, 2497, 2680
Bse1I ACTGG 7 cut(s) 377, 703, 1358, 1945, 2507, 2626, 2668
Bse21I CCTNAGG 3 cut(s) 414, 924, 2696
Bse3DI GCAATG 3 cut(s) 145, 2321, 2874
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 3 cut(s) 156, 792, 1349
BseCI ATCGAT 1 cut(s) 3021
BseDI CCNNGG 3 cut(s) 1458, 2214, 2514
BseGI GGATG 6 cut(s) 33, 1192, 1264, 1468, 1570, 2637
BseLI CCNNNNNNNGG 7 cut(s) 698, 815, 968, 1165, 1303, 2497, 2680
BseMI GCAATG 3 cut(s) 145, 2321, 2874
BseMII CTCAG 6 cut(s) 405, 915, 1716, 1718, 1987, 2687
BseNI ACTGG 7 cut(s) 377, 703, 1358, 1945, 2507, 2626, 2668
BseRI GAGGAG 2 cut(s) 515, 2327
BseSI GKGCMC 2 cut(s) 305, 881
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 2 cut(s) 1611, 2016
BshFI GGCC 5 cut(s) 807, 1011, 1658, 2513, 2882
BshNI GGYRCC 3 cut(s) 300, 1435, 2569
BshVI ATCGAT 1 cut(s) 3021
BsiHKCI CYCGRG 3 cut(s) 1781, 2106, 2984
BsiSI CCGG 3 cut(s) 528, 692, 1239
BslFI GGGAC 4 cut(s) 408, 1280, 1961, 2684
BslI CCNNNNNNNGG 7 cut(s) 698, 815, 968, 1165, 1303, 2497, 2680
BsmAI GTCTC 7 cut(s) 148, 689, 986, 1931, 2084, 2590, 2807
BsmBI CGTCTC 1 cut(s) 2590
BsmFI GGGAC 4 cut(s) 408, 1280, 1961, 2684
BsnI GGCC 5 cut(s) 807, 1011, 1658, 2513, 2882
Bso31I GGTCTC 1 cut(s) 986
BsoBI CYCGRG 3 cut(s) 1781, 2106, 2984
Bsp1286I GDGCHC 2 cut(s) 305, 881
Bsp13I TCCGGA 1 cut(s) 691
Bsp143I GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
BspACI CCGC 9 cut(s) 356, 485, 746, 1156, 1304, 1944, 2498, 2782, 2888
BspANI GGCC 5 cut(s) 807, 1011, 1658, 2513, 2882
BspCNI CTCAG 6 cut(s) 406, 916, 1717, 1717, 1986, 2688
BspDI ATCGAT 1 cut(s) 3021
BspEI TCCGGA 1 cut(s) 691
BspHI TCATGA 1 cut(s) 2773
BspLI GGNNCC 6 cut(s) 302, 960, 1437, 2571, 2672, 2764
BspMAI CTGCAG 2 cut(s) 1005, 1303
BspMI ACCTGC 3 cut(s) 288, 1093, 2881
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 3 cut(s) 2757, 2770, 3026
BspT107I GGYRCC 3 cut(s) 300, 1435, 2569
BspTNI GGTCTC 1 cut(s) 986
BsrDI GCAATG 3 cut(s) 145, 2321, 2874
BsrI ACTGG 7 cut(s) 377, 703, 1358, 1945, 2507, 2626, 2668
BssECI CCNNGG 3 cut(s) 1458, 2214, 2514
BssMI GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
BssNI GRCGYC 3 cut(s) 113, 1436, 2778
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 2214
Bst2BI CACGAG 1 cut(s) 36
Bst2UI CCWGG 3 cut(s) 156, 792, 1349
Bst4CI ACNGT 7 cut(s) 883, 1318, 1775, 2087, 2175, 2560, 2578
Bst6I CTCTTC 7 cut(s) 924, 1407, 1536, 2196, 2297, 2411, 2453
BstACI GRCGYC 3 cut(s) 113, 1436, 2778
BstBAI YACGTR 1 cut(s) 2612
BstDEI CTNAG 6 cut(s) 414, 924, 1704, 1725, 1973, 2696
BstDSI CCRYGG 2 cut(s) 1458, 2514
BstENI CCTNNNNNAGG 1 cut(s) 2678
BstF5I GGATG 6 cut(s) 33, 1192, 1264, 1468, 1570, 2637
BstH2I RGCGCY 1 cut(s) 1439
BstHHI GCGC 3 cut(s) 1438, 1484, 2430
BstKTI GATC 7 cut(s) 542, 2248, 2276, 2765, 2863, 3021, 3025
BstMAI GTCTC 7 cut(s) 148, 689, 986, 1931, 2084, 2590, 2807
BstMBI GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
BstNI CCWGG 3 cut(s) 156, 792, 1349
BstNSI RCATGY 2 cut(s) 602, 2163
BstSCI CCNGG 4 cut(s) 154, 527, 790, 1347
BstSFI CTRYAG 7 cut(s) 339, 1001, 1299, 1389, 2184, 2538, 2577
BstSLI GKGCMC 2 cut(s) 305, 881
BstV2I GAAGAC 2 cut(s) 1680, 2919
BstX2I RGATCY 2 cut(s) 2245, 2762
BstXI CCANNNNNNTGG 2 cut(s) 554, 2152
BstYI RGATCY 2 cut(s) 2245, 2762
Bsu15I ATCGAT 1 cut(s) 3021
Bsu36I CCTNAGG 3 cut(s) 414, 924, 2696
BsuI GTATCC 1 cut(s) 2914
BsuRI GGCC 5 cut(s) 807, 1011, 1658, 2513, 2882
BsuTUI ATCGAT 1 cut(s) 3021
BtgI CCRYGG 2 cut(s) 1458, 2514
BtrI CACGTC 1 cut(s) 1515
BtsCI GGATG 6 cut(s) 33, 1192, 1264, 1468, 1570, 2637
BtsI GCAGTG 4 cut(s) 1125, 1237, 1801, 2019
BtsIMutI CAGTG 6 cut(s) 879, 1125, 1237, 1801, 2019, 2514
BveI ACCTGC 3 cut(s) 288, 1093, 2881
CaiI CAGNNNCTG 4 cut(s) 347, 395, 1904, 1940
CciI TCATGA 1 cut(s) 2773
CfoI GCGC 3 cut(s) 1438, 1484, 2430
Cfr13I GGNCC 6 cut(s) 788, 806, 868, 1345, 2671, 2968
ClaI ATCGAT 1 cut(s) 3021
CseI GACGC 3 cut(s) 121, 710, 2786
CsiI ACCWGGT 1 cut(s) 790
CspCI CAANNNNNGTGG 2 cut(s) 2490, 2525
CviAII CATG 7 cut(s) 20, 599, 985, 2146, 2160, 2774, 2929
DdeI CTNAG 6 cut(s) 414, 924, 1704, 1725, 1973, 2696
DinI GGCGCC 1 cut(s) 1437
DpnI GATC 7 cut(s) 541, 2247, 2275, 2764, 2862, 3020, 3024
DpnII GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
DrdI GACNNNNNNGTC 1 cut(s) 1103
DseDI GACNNNNNNGTC 1 cut(s) 1103
EaeI YGGCCR 2 cut(s) 1009, 2511
Eam1104I CTCTTC 7 cut(s) 924, 1407, 1536, 2196, 2297, 2411, 2453
EarI CTCTTC 7 cut(s) 924, 1407, 1536, 2196, 2297, 2411, 2453
EciI GGCGGA 1 cut(s) 1145
Eco130I CCWWGG 1 cut(s) 2214
Eco31I GGTCTC 1 cut(s) 986
Eco32I GATATC 1 cut(s) 1429
Eco47I GGWCC 5 cut(s) 788, 868, 1345, 2671, 2968
Eco81I CCTNAGG 3 cut(s) 414, 924, 2696
Eco88I CYCGRG 3 cut(s) 1781, 2106, 2984
EcoNI CCTNNNNNAGG 1 cut(s) 2678
EcoO109I RGGNCCY 3 cut(s) 868, 2671, 2968
EcoRII CCWGG 3 cut(s) 154, 790, 1347
EcoRV GATATC 1 cut(s) 1429
EcoT14I CCWWGG 1 cut(s) 2214
EgeI GGCGCC 1 cut(s) 1437
EheI GGCGCC 1 cut(s) 1437
ErhI CCWWGG 1 cut(s) 2214
Esp3I CGTCTC 1 cut(s) 2590
FaeI CATG 7 cut(s) 23, 602, 988, 2149, 2163, 2777, 2932
FalI AAGNNNNNCTT 2 cut(s) 2232, 2264
FaqI GGGAC 4 cut(s) 408, 1280, 1961, 2684
FatI CATG 7 cut(s) 19, 598, 984, 2145, 2159, 2773, 2928
FauI CCCGC 3 cut(s) 1937, 2789, 2895
FokI GGATG 6 cut(s) 40, 1199, 1271, 1475, 1577, 2644
FspBI CTAG 8 cut(s) 75, 872, 1365, 1382, 1494, 1679, 2075, 2864
GlaI GCGC 3 cut(s) 1437, 1483, 2429
GsaI CCCAGC 1 cut(s) 429
GsuI CTGGAG 2 cut(s) 2643, 2685
HaeII RGCGCY 1 cut(s) 1439
HaeIII GGCC 5 cut(s) 807, 1011, 1658, 2513, 2882
HapII CCGG 3 cut(s) 528, 692, 1239
HgaI GACGC 3 cut(s) 121, 710, 2786
HhaI GCGC 3 cut(s) 1438, 1484, 2430
Hin1I GRCGYC 3 cut(s) 113, 1436, 2778
Hin1II CATG 7 cut(s) 23, 602, 988, 2149, 2163, 2777, 2932
Hin6I GCGC 3 cut(s) 1436, 1482, 2428
HinP1I GCGC 3 cut(s) 1436, 1482, 2428
HincII GTYRAC 2 cut(s) 725, 2287
HindII GTYRAC 2 cut(s) 725, 2287
HpaII CCGG 3 cut(s) 528, 692, 1239
Hpy166II GTNNAC 8 cut(s) 725, 788, 1771, 1847, 2083, 2287, 2806, 2935
Hpy8I GTNNAC 8 cut(s) 725, 788, 1771, 1847, 2083, 2287, 2806, 2935
Hpy99I CGWCG 1 cut(s) 1519
HpyCH4III ACNGT 7 cut(s) 883, 1318, 1775, 2087, 2175, 2560, 2578
HpyCH4IV ACGT 7 cut(s) 624, 633, 727, 981, 1023, 1514, 2611
HpyF3I CTNAG 6 cut(s) 414, 924, 1704, 1725, 1973, 2696
HpySE526I ACGT 7 cut(s) 624, 633, 727, 981, 1023, 1514, 2611
Hsp92I GRCGYC 3 cut(s) 113, 1436, 2778
Hsp92II CATG 7 cut(s) 23, 602, 988, 2149, 2163, 2777, 2932
HspAI GCGC 3 cut(s) 1436, 1482, 2428
KasI GGCGCC 1 cut(s) 1435
Kpn2I TCCGGA 1 cut(s) 691
Kzo9I GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
LmnI GCTCC 7 cut(s) 502, 575, 1901, 2125, 2314, 2624, 3039
LweI GCATC 7 cut(s) 319, 376, 430, 471, 651, 751, 2223
MabI ACCWGGT 1 cut(s) 790
MaeI CTAG 8 cut(s) 75, 872, 1365, 1382, 1494, 1679, 2075, 2864
MaeII ACGT 7 cut(s) 624, 633, 727, 981, 1023, 1514, 2611
MaeIII GTNAC 6 cut(s) 620, 670, 728, 1054, 2111, 2209
MalI GATC 7 cut(s) 541, 2247, 2275, 2764, 2862, 3020, 3024
MboI GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
MfeI CAATTG 4 cut(s) 184, 608, 1371, 2226
MflI RGATCY 2 cut(s) 2245, 2762
MhlI GDGCHC 2 cut(s) 305, 881
MlsI TGGCCA 2 cut(s) 1011, 2513
MluNI TGGCCA 2 cut(s) 1011, 2513
Mly113I GGCGCC 1 cut(s) 1436
MlyI GAGTC 6 cut(s) 76, 365, 585, 1091, 1244, 2470
MmeI TCCRAC 4 cut(s) 616, 1074, 1236, 1556
Mox20I TGGCCA 2 cut(s) 1011, 2513
MroI TCCGGA 1 cut(s) 691
MroXI GAANNNNTTC 5 cut(s) 1083, 1139, 2038, 2193, 2916
MscI TGGCCA 2 cut(s) 1011, 2513
MseI TTAA 4 cut(s) 191, 1956, 2343, 2601
MslI CAYNNNNRTG 3 cut(s) 1751, 2150, 2411
Msp20I TGGCCA 2 cut(s) 1011, 2513
MspA1I CMGCKG 2 cut(s) 1304, 1885
MspI CCGG 3 cut(s) 528, 692, 1239
MspR9I CCNGG 4 cut(s) 156, 529, 792, 1349
MunI CAATTG 4 cut(s) 184, 608, 1371, 2226
MvaI CCWGG 3 cut(s) 156, 792, 1349
NarI GGCGCC 1 cut(s) 1436
NciI CCSGG 1 cut(s) 529
NdeII GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 7 cut(s) 23, 602, 988, 2149, 2163, 2777, 2932
NlaIV GGNNCC 6 cut(s) 302, 960, 1437, 2571, 2672, 2764
NmuCI GTSAC 4 cut(s) 670, 728, 2111, 2209
NspI RCATGY 2 cut(s) 602, 2163
OliI CACNNNNGTG 1 cut(s) 1751
PaeI GCATGC 1 cut(s) 2163
PagI TCATGA 1 cut(s) 2773
PaqCI CACCTGC 2 cut(s) 288, 2881
PciI ACATGT 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PdmI GAANNNNTTC 5 cut(s) 1083, 1139, 2038, 2193, 2916
PfeI GAWTC 5 cut(s) 324, 705, 939, 1708, 3003
PflFI GACNNNGTC 1 cut(s) 2372
PleI GAGTC 6 cut(s) 76, 365, 585, 1091, 1243, 2469
PluTI GGCGCC 1 cut(s) 1439
PpsI GAGTC 6 cut(s) 76, 365, 585, 1091, 1243, 2469
Ppu21I YACGTR 1 cut(s) 2612
PpuMI RGGWCCY 3 cut(s) 868, 2671, 2968
PscI ACATGT 1 cut(s) 598
Psp5II RGGWCCY 3 cut(s) 868, 2671, 2968
Psp6I CCWGG 3 cut(s) 154, 790, 1347
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 3 cut(s) 154, 790, 1347
PspN4I GGNNCC 6 cut(s) 302, 960, 1437, 2571, 2672, 2764
PspPI GGNCC 6 cut(s) 788, 806, 868, 1345, 2671, 2968
PspPPI RGGWCCY 3 cut(s) 868, 2671, 2968
PsrI GAACNNNNNNTAC 2 cut(s) 151, 183
PstI CTGCAG 2 cut(s) 1005, 1303
PstNI CAGNNNCTG 4 cut(s) 347, 395, 1904, 1940
PsuI RGATCY 2 cut(s) 2245, 2762
PsyI GACNNNGTC 1 cut(s) 2372
PvuII CAGCTG 1 cut(s) 1885
RseI CAYNNNNRTG 3 cut(s) 1751, 2150, 2411
SaqAI TTAA 4 cut(s) 191, 1956, 2343, 2601
Sau3AI GATC 7 cut(s) 539, 2245, 2273, 2762, 2860, 3018, 3022
Sau96I GGNCC 6 cut(s) 788, 806, 868, 1345, 2671, 2968
SchI GAGTC 6 cut(s) 76, 365, 585, 1091, 1244, 2470
ScrFI CCNGG 4 cut(s) 156, 529, 792, 1349
SduI GDGCHC 2 cut(s) 305, 881
SexAI ACCWGGT 1 cut(s) 790
SfaNI GCATC 7 cut(s) 319, 376, 430, 471, 651, 751, 2223
SfcI CTRYAG 7 cut(s) 339, 1001, 1299, 1389, 2184, 2538, 2577
SfoI GGCGCC 1 cut(s) 1437
SinI GGWCC 5 cut(s) 788, 868, 1345, 2671, 2968
SmiMI CAYNNNNRTG 3 cut(s) 1751, 2150, 2411
SmlI CTYRAG 7 cut(s) 435, 1557, 2166, 2309, 2327, 2675, 2951
SmoI CTYRAG 7 cut(s) 435, 1557, 2166, 2309, 2327, 2675, 2951
SphI GCATGC 1 cut(s) 2163
SsiI CCGC 9 cut(s) 356, 485, 746, 1156, 1304, 1944, 2498, 2782, 2888
SspDI GGCGCC 1 cut(s) 1435
SspMI CTAG 8 cut(s) 75, 872, 1365, 1382, 1494, 1679, 2075, 2864
StyD4I CCNGG 4 cut(s) 154, 527, 790, 1347
StyI CCWWGG 1 cut(s) 2214
TaaI ACNGT 7 cut(s) 883, 1318, 1775, 2087, 2175, 2560, 2578
TaiI ACGT 7 cut(s) 627, 636, 730, 984, 1026, 1517, 2614
TaqI TCGA 5 cut(s) 209, 263, 2830, 2910, 3021
TatI WGTACW 4 cut(s) 3, 1367, 1448, 2170
TauI GCSGC 3 cut(s) 487, 748, 2501
TfiI GAWTC 5 cut(s) 324, 705, 939, 1708, 3003
Tru1I TTAA 4 cut(s) 191, 1956, 2343, 2601
Tru9I TTAA 4 cut(s) 191, 1956, 2343, 2601
TscAI CASTG 6 cut(s) 886, 1132, 1237, 1801, 2026, 2514
TseFI GTSAC 4 cut(s) 670, 728, 2111, 2209
Tsp45I GTSAC 4 cut(s) 670, 728, 2111, 2209
TspDTI ATGAA 8 cut(s) 1082, 1203, 1275, 1326, 2031, 2329, 2631, 2762
TspGWI ACGGA 6 cut(s) 114, 1016, 1475, 1709, 1979, 2531
TspRI CASTG 6 cut(s) 886, 1132, 1237, 1801, 2026, 2514
Tth111I GACNNNGTC 1 cut(s) 2372
VpaK11BI GGWCC 5 cut(s) 788, 868, 1345, 2671, 2968
XagI CCTNNNNNAGG 1 cut(s) 2678
XapI RAATTY 5 cut(s) 197, 889, 1527, 1614, 2839
XceI RCATGY 2 cut(s) 602, 2163
XcmI CCANNNNNNNNNTGG 1 cut(s) 1465
XmnI GAANNNNTTC 5 cut(s) 1083, 1139, 2038, 2193, 2916
XspI CTAG 8 cut(s) 75, 872, 1365, 1382, 1494, 1679, 2075, 2864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.