pycom02g19580

Rgp1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007398
Physical Location & Seq
Forward (+)
17467875 .. 17474118
6244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGTTGCCGCGCAGGTTATCCGCCATTTTCGGAGGCCTCGGTTCGAACGCCAAGACAGTGACCAGCGAGCTCCTCCCGACTCTCAAGCTCCAAACCGACAAGGAAGTCTACAGGCCCGGCGATCCCATCGTCATCACCATCGAGATTTCGAACCCTAGCAGCAATGACTGTTCGGCTTACTCGCTCTTGATCGAGCGCCTATGGTTTGAGATTAAGGGGATTGAGAAGCTCGATTCTCAGTGGTTCGCCACGCAGAAACCGACGGCTGGATCCAGACAGAGGAGAGGTGAATATGTGTTCATGGAGTGCTCGACTCCCGCCCTGGTTACGAATCAGATTGTTTCACCCGGGGGTACGAAATCATATGTTGTGCGGTCGTTGCTGGCCCCCGTTATACCACCATCGTACAAAGGTGCTACCCTTCGATATATGTACTATGTTAGAAGTACAATGTCTGGACAATGGCTGATATTGGAGAATGCCCACTCTCGTGGAGAATCAGTGAAAGATTTTCCTGAAATGGAAGCTCGTGTTCCAGTACAAGTATGGGTCACTCAGAAAACCAGTGGATTGGGAATGGAAGGTCTTACAGATGGGATTGTACCTTCTGTAACCATCCAAATGGATATGTATTGGAAAGAGATGGATGGGGACTCTGATTGGGTGAGAGTAAATGAAACATATGATGGGGTTGAAGAAGGGTATGAAAGCTCAAGGGATGAGATCTCATCCGTTTCTTCGTACAATCCCATGAAAGAACATTTAAACAGAACATTCGGAAGTTCACTATCCTTGCAGTCCCAACGGTCTTCAAATAAGGATAACGCCTATCTTGAAGGAGAACGTACGAGTTTATCTTCAAATCTTGCAATCCCACGGCTCTCTATGGCTGAAGTCTTATATGATACTGGTGCTAATACTTCATTGCCCCCGATTTCATCTGCTATTGGCTCTCCAAGCCAGCAGCAGAATCTCACAAAGCGCCTTTCTTTGGATGATGAAGCAAGGCCATCTTCTTCACCAGCATCTGGAGCAGTTGAAACCTTAGCATCAGAGGGCTTCATTCGAGGGAGGTCATACAATATCCGGCTGGATGACCGAGTTCTGCTTAGATTTTCCCCAAAAAATTCTGATTCCAATTATTACTTCTGTGATATGATTGGTGGAACTCTTACTTTCTTTCATGAAGAAGGAGCTAGAAGATGCCTTGAGGTTTCTATAACATTGGAGACTTCAGAAACTATTAGCCAGCGCTTTGTGCATCCTTCTCGGAGGAATTCTCCCACAATTACAAAGATCCAGAGTGATCATTATGAGGTTGTAGCGGACTTGGTGCAGACAAGTTTTCTTTTCTCCATTCCCATGGATGGGCCTATGTCTTTTTCCACTCCCCATGTTTCTGTACAATGGGAACTTCGATTTGAATTTTTTACCACTCCGAAGAATGTGGATTGGACCAGTATTGTACGAGCATCCTCTTTTGATAGAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rgp1

Protein Analysis

498

Amino Acids

55.55

Weight (kDa)

5.42

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rgp1 PF08737 114 - 478 6.8e-15 Rgp1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 3
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 10
AciI CCGC 5 cut(s) 8, 21, 318, 373, 1325
AclWI GGATC 4 cut(s) 116, 264, 277, 1291
AcsI RAATTY 3 cut(s) 1126, 1276, 1424
AcuI CTGAAG 2 cut(s) 912, 1218
AfeI AGCGCT 1 cut(s) 1253
AfiI CCNNNNNNNGG 6 cut(s) 266, 279, 991, 1028, 1367, 1368
AgsI TTSAA 6 cut(s) 695, 813, 836, 861, 1040, 1424
AjnI CCWGG 1 cut(s) 321
AjuI GAANNNNNNNTTGG 2 cut(s) 1130, 1162
AleI CACNNNNGTG 1 cut(s) 489
AluBI AGCT 6 cut(s) 70, 88, 229, 527, 711, 1196
AluI AGCT 6 cut(s) 70, 88, 229, 527, 711, 1196
Alw21I GWGCWC 2 cut(s) 72, 311
Alw26I GTCTC 1 cut(s) 1223
AlwI GGATC 4 cut(s) 116, 264, 277, 1291
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 1 cut(s) 347
Aor51HI AGCGCT 1 cut(s) 1253
AoxI GGCC 5 cut(s) 34, 113, 384, 1007, 1370
ApeKI GCWGC 2 cut(s) 159, 964
ApoI RAATTY 3 cut(s) 1126, 1276, 1424
AspLEI GCGC 4 cut(s) 12, 198, 984, 1254
AspS9I GGNCC 4 cut(s) 114, 385, 1370, 1455
AsuC2I CCSGG 3 cut(s) 117, 348, 349
AsuHPI GGTGA 5 cut(s) 127, 299, 336, 676, 1011
AsuII TTCGAA 2 cut(s) 44, 149
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 1455
BamHI GGATCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 2 cut(s) 489, 528
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 2 cut(s) 72, 311
BbvI GCAGC 2 cut(s) 171, 976
BceAI ACGGC 2 cut(s) 279, 893
BcgI CGANNNNNNTGC 2 cut(s) 1250, 1284
BciT130I CCWGG 1 cut(s) 323
BclI TGATCA 1 cut(s) 1306
BcnI CCSGG 3 cut(s) 117, 348, 349
BcoDI GTCTC 1 cut(s) 1223
BfaI CTAG 2 cut(s) 156, 1197
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 3 cut(s) 199, 985, 1255
BfuAI ACCTGC 1 cut(s) 3
BglII AGATCT 1 cut(s) 723
BisI GCNGC 3 cut(s) 8, 160, 965
BlsI GCNGC 3 cut(s) 9, 161, 966
Bme1390I CCNGG 4 cut(s) 117, 323, 348, 349
Bme18I GGWCC 1 cut(s) 1455
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 4 cut(s) 114, 385, 1370, 1455
BmiI GGNNCC 2 cut(s) 271, 387
BmrFI CCNGG 4 cut(s) 117, 323, 348, 349
BmsI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1481
BpiI GAAGAC 1 cut(s) 801
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 1 cut(s) 1050
Bpu10I CCTNAGC 1 cut(s) 1045
Bpu14I TTCGAA 2 cut(s) 44, 149
BpuEI CTTGAG 3 cut(s) 68, 697, 1229
BpuMI CCSGG 3 cut(s) 117, 348, 349
BsaJI CCNNGG 6 cut(s) 37, 321, 347, 348, 875, 1362
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 279, 991, 1028, 1367, 1368
Bse1I ACTGG 4 cut(s) 536, 564, 913, 1458
Bse3DI GCAATG 2 cut(s) 169, 923
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 6 cut(s) 37, 321, 347, 348, 875, 1362
BseGI GGATG 9 cut(s) 615, 652, 724, 728, 1000, 1099, 1261, 1372, 1472
BseLI CCNNNNNNNGG 6 cut(s) 266, 279, 991, 1028, 1367, 1368
BseMI GCAATG 2 cut(s) 169, 923
BseMII CTCAG 2 cut(s) 251, 569
BseNI ACTGG 4 cut(s) 536, 564, 913, 1458
BseRI GAGGAG 2 cut(s) 62, 295
BseXI GCAGC 2 cut(s) 171, 976
BsgI GTGCAG 1 cut(s) 1355
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 377
BshFI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BsiEI CGRYCG 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 72, 311
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 3 cut(s) 117, 348, 1087
BsiWI CGTACG 1 cut(s) 845
BslFI GGGAC 2 cut(s) 665, 784
BslI CCNNNNNNNGG 6 cut(s) 266, 279, 991, 1028, 1367, 1368
BsmAI GTCTC 1 cut(s) 1223
BsmFI GGGAC 2 cut(s) 665, 784
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 2 cut(s) 44, 149
Bsp1286I GDGCHC 2 cut(s) 72, 311
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 5 cut(s) 8, 21, 318, 373, 1325
BspANI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BspCNI CTCAG 2 cut(s) 250, 568
BspFNI CGCG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 2 cut(s) 271, 387
BspMI ACCTGC 1 cut(s) 3
BspPI GGATC 4 cut(s) 116, 264, 277, 1291
BspT104I TTCGAA 2 cut(s) 44, 149
BsrDI GCAATG 2 cut(s) 169, 923
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 4 cut(s) 536, 564, 913, 1458
BssECI CCNNGG 6 cut(s) 37, 321, 347, 348, 875, 1362
BssMI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
BssSI CACGAG 2 cut(s) 489, 528
BssT1I CCWWGG 1 cut(s) 1362
Bst2BI CACGAG 2 cut(s) 489, 528
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 3 cut(s) 58, 170, 807
BstAUI TGTACA 1 cut(s) 1402
BstBI TTCGAA 2 cut(s) 44, 149
BstC8I GCNNGC 4 cut(s) 68, 384, 962, 1250
BstDEI CTNAG 4 cut(s) 237, 555, 1045, 1109
BstDSI CCRYGG 2 cut(s) 875, 1362
BstF5I GGATG 9 cut(s) 615, 652, 724, 728, 1000, 1099, 1261, 1372, 1472
BstFNI CGCG 1 cut(s) 10
BstH2I RGCGCY 3 cut(s) 199, 985, 1255
BstHHI GCGC 4 cut(s) 12, 198, 984, 1254
BstKTI GATC 6 cut(s) 124, 192, 272, 726, 1299, 1309
BstMAI GTCTC 1 cut(s) 1223
BstMBI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
BstMCI CGRYCG 1 cut(s) 377
BstMWI GCNNNNNNNGC 4 cut(s) 379, 957, 1031, 1258
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 4 cut(s) 115, 321, 346, 347
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 171, 976
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 3 cut(s) 269, 723, 1296
BstXI CCANNNNNNTGG 4 cut(s) 491, 571, 622, 1363
BstYI RGATCY 3 cut(s) 269, 723, 1296
BsuRI GGCC 5 cut(s) 36, 115, 386, 1009, 1372
BtgI CCRYGG 2 cut(s) 875, 1362
BtsCI GGATG 9 cut(s) 615, 652, 724, 728, 1000, 1099, 1261, 1372, 1472
BtsIMutI CAGTG 4 cut(s) 63, 245, 507, 571
BveI ACCTGC 1 cut(s) 3
Cac8I GCNNGC 4 cut(s) 68, 384, 962, 1250
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 4 cut(s) 12, 198, 984, 1254
Cfr13I GGNCC 4 cut(s) 114, 385, 1370, 1455
Cfr9I CCCGGG 1 cut(s) 347
CviAII CATG 5 cut(s) 301, 751, 1184, 1363, 1394
DdeI CTNAG 4 cut(s) 237, 555, 1045, 1109
DpnI GATC 6 cut(s) 123, 191, 271, 725, 1298, 1308
DpnII GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
DraI TTTAAA 1 cut(s) 765
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 1362
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 1455
Eco47III AGCGCT 1 cut(s) 1253
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 912, 1218
Eco88I CYCGRG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 1362
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 1362
FaeI CATG 5 cut(s) 304, 754, 1187, 1366, 1397
FalI AAGNNNNNCTT 2 cut(s) 997, 1029
FaqI GGGAC 2 cut(s) 665, 784
FatI CATG 5 cut(s) 300, 750, 1183, 1362, 1393
FauI CCCGC 1 cut(s) 325
FauNDI CATATG 2 cut(s) 364, 682
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 8, 160, 965
FokI GGATG 9 cut(s) 602, 659, 715, 731, 1007, 1106, 1248, 1379, 1459
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 8, 160, 965
FspBI CTAG 2 cut(s) 156, 1197
GlaI GCGC 4 cut(s) 11, 197, 983, 1253
GluI GCNGC 3 cut(s) 8, 160, 965
GsuI CTGGAG 1 cut(s) 1050
HaeII RGCGCY 3 cut(s) 199, 985, 1255
HaeIII GGCC 5 cut(s) 36, 115, 386, 1009, 1372
HapII CCGG 3 cut(s) 117, 348, 1087
HhaI GCGC 4 cut(s) 12, 198, 984, 1254
Hin1II CATG 5 cut(s) 304, 754, 1187, 1366, 1397
Hin6I GCGC 4 cut(s) 10, 196, 982, 1252
HinP1I GCGC 4 cut(s) 10, 196, 982, 1252
HinfI GANTC 8 cut(s) 79, 233, 313, 331, 497, 653, 970, 1133
HpaII CCGG 3 cut(s) 117, 348, 1087
HphI GGTGA 5 cut(s) 127, 299, 336, 676, 1011
Hpy166II GTNNAC 2 cut(s) 109, 785
Hpy8I GTNNAC 2 cut(s) 109, 785
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 7 cut(s) 431, 575, 615, 692, 830, 1184, 1275
HpyCH4III ACNGT 3 cut(s) 58, 170, 807
HpyCH4IV ACGT 1 cut(s) 844
HpyCH4V TGCA 4 cut(s) 796, 869, 1261, 1336
HpyF10VI GCNNNNNNNGC 4 cut(s) 379, 957, 1031, 1258
HpyF3I CTNAG 4 cut(s) 237, 555, 1045, 1109
HpySE526I ACGT 1 cut(s) 844
Hsp92II CATG 5 cut(s) 304, 754, 1187, 1366, 1397
HspAI GCGC 4 cut(s) 10, 196, 982, 1252
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
LmnI GCTCC 4 cut(s) 75, 93, 1031, 1193
Lsp1109I GCAGC 2 cut(s) 171, 976
LweI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1481
MaeI CTAG 2 cut(s) 156, 1197
MaeII ACGT 1 cut(s) 844
MaeIII GTNAC 4 cut(s) 58, 325, 550, 610
MalI GATC 6 cut(s) 123, 191, 271, 725, 1298, 1308
MboI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
MboII GAAGA 9 cut(s) 707, 729, 801, 849, 1005, 1008, 1199, 1212, 1453
MflI RGATCY 3 cut(s) 269, 723, 1296
MhlI GDGCHC 2 cut(s) 72, 311
MluCI AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
MlyI GAGTC 3 cut(s) 73, 307, 647
MseI TTAA 2 cut(s) 213, 764
MslI CAYNNNNRTG 3 cut(s) 489, 620, 1361
MspI CCGG 3 cut(s) 117, 348, 1087
MspR9I CCNGG 4 cut(s) 117, 323, 348, 349
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 379, 957, 1031, 1258
NciI CCSGG 3 cut(s) 117, 348, 349
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 2 cut(s) 364, 682
NdeII GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
NlaIII CATG 5 cut(s) 304, 754, 1187, 1366, 1397
NlaIV GGNNCC 2 cut(s) 271, 387
NmuCI GTSAC 2 cut(s) 58, 550
NspV TTCGAA 2 cut(s) 44, 149
OliI CACNNNNGTG 1 cut(s) 489
PagI TCATGA 1 cut(s) 1183
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PctI GAATGC 1 cut(s) 484
PfeI GAWTC 5 cut(s) 233, 331, 497, 970, 1133
Pfl23II CGTACG 1 cut(s) 845
PflMI CCANNNNNTGG 1 cut(s) 1028
PkrI GCNGC 3 cut(s) 9, 161, 966
PleI GAGTC 3 cut(s) 73, 307, 647
PpsI GAGTC 3 cut(s) 73, 307, 647
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspLI CGTACG 1 cut(s) 845
PspN4I GGNNCC 2 cut(s) 271, 387
PspPI GGNCC 4 cut(s) 114, 385, 1370, 1455
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 3 cut(s) 269, 723, 1296
RseI CAYNNNNRTG 3 cut(s) 489, 620, 1361
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 213, 764
SatI GCNGC 3 cut(s) 8, 160, 965
Sau3AI GATC 6 cut(s) 121, 189, 269, 723, 1296, 1306
Sau96I GGNCC 4 cut(s) 114, 385, 1370, 1455
SchI GAGTC 3 cut(s) 73, 307, 647
ScrFI CCNGG 4 cut(s) 117, 323, 348, 349
SduI GDGCHC 2 cut(s) 72, 311
SfaNI GCATC 5 cut(s) 1034, 1058, 1193, 1270, 1481
SfcI CTRYAG 1 cut(s) 109
SfuI TTCGAA 2 cut(s) 44, 149
SinI GGWCC 1 cut(s) 1455
SmaI CCCGGG 1 cut(s) 349
SmiMI CAYNNNNRTG 3 cut(s) 489, 620, 1361
SmlI CTYRAG 3 cut(s) 83, 712, 1208
SmoI CTYRAG 3 cut(s) 83, 712, 1208
Sse9I AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 5 cut(s) 8, 21, 318, 373, 1325
SspMI CTAG 2 cut(s) 156, 1197
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 4 cut(s) 115, 321, 346, 347
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 3 cut(s) 58, 170, 807
TaiI ACGT 1 cut(s) 847
TaqI TCGA 9 cut(s) 44, 141, 149, 192, 231, 311, 424, 1066, 1417
TaqII GACCGA 1 cut(s) 1113
TasI AATT 5 cut(s) 1126, 1138, 1276, 1287, 1424
TatI WGTACW 4 cut(s) 432, 446, 538, 1402
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 5 cut(s) 233, 331, 497, 970, 1133
Tru1I TTAA 2 cut(s) 213, 764
Tru9I TTAA 2 cut(s) 213, 764
TscAI CASTG 4 cut(s) 63, 245, 507, 571
TseFI GTSAC 2 cut(s) 58, 550
TseI GCWGC 2 cut(s) 159, 964
Tsp45I GTSAC 2 cut(s) 58, 550
TspGWI ACGGA 1 cut(s) 721
TspMI CCCGGG 1 cut(s) 347
TspRI CASTG 4 cut(s) 63, 245, 507, 571
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 1455
XapI RAATTY 3 cut(s) 1126, 1276, 1424
XcmI CCANNNNNNNNNTGG 1 cut(s) 543
XmaI CCCGGG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 2 cut(s) 156, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.