pycom02g21690
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010940
Physical Location & Seq
Reverse (-)
19914605 .. 19919050
4446 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGGCCGACGAGTCGCAGAAATGGGTGCTGATGGTGACGGCTCAGACGCCCACCAACATCGCCGTGATCAAGTACTGGGGGAAGAGGGACGAGAAGCTCATCTTGCCAGTCAATGACAGCATCAGCGTGACGCTTGATCCTGCGCACCTTTGCACCACCACCACTGTCTCCGTCAGTCCCACCTTTGACCAAGATCGGATGTGGCTCAATGGCGAGGAGATTTCCATTTCCGGAGGCAGATTCCAAAGCTGTTTAAGAGAAATTCGCAGTCGAGCTACCGATATTGATGATAAGGAAAAGGGAATCAATATTACTAAGAAGGACTGGGAAAAGTTGCATGTACATATTTCTTCATATAACAATTTTCCTACTGCTGCTGGATTGGCTTCCTCAGCAGCTGGTTTTGCTTGTCTAGTTTTTGCTCTCGGAAAGCTAATGAACGTGAAAGAAGATCACAGCCAGCTTTCTGCTATTGCAAGGCAAGGTTCTGGTAGTGCTTGCCGCAGCTTGTATGGGGGATTTGTCAAGTGGATCATGGGAAAAGAAGAGAAGGGAACCGACAGTCTTGCAGTCCAACTTGCAGATGAGAAGCACTGGGACGAGCTTGTTATTGTTATCGCTGTGGTAAGTTCACGACAGAAGGAAACAAGTAGCACTACAGGAATGCGTGATACTGTTGAAACAAGTTTGCTCTTACAACATAGAGCAAAGGAAGTAGTACCAAAACGCATATTAAAAATGGACGAAGCCATAAAAAATCGTGATTTTGCTTCTTTTGCACAACTGACCTGTACTGACAGTAACCAGTTTCATGCTGTCTGCCTGGACACATGTCCACCCATATTCTACATGAATGATACATCCCACAGGCAAGCCCCGATAATAAGCCTTGTTGAAAAGTGGAACAAAGCAGAAGGAACACCTCAGGTGGCTTATACATTTGATGCGGGGCCAAATGCGGTTCTAATCGCACGTAACAGAAAAACAGCTGCCCTTTTGATTCAGAAGTTGCTATTCTGCTTTCCACCAAATTCTGATGCAGATTTGAGCAGTTTCGTTCTTGGCGATAAGTCAATTCTAAAAGATGCCGGGGTTGAGAGCCCGGAGGATATTGAAACTTTACCCGCACCTCCAGAAATCAAGGACCCGTCCCAGAAATTCCGAGGAGATGTCAGTTACTTTATTTGCACAAGACCCGGGAGAGGTCCAGTTGTGCTGTCAGACGAAAGTCATCTTCTCAACCCTGAAACTGGATTGCCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

422

Amino Acids

46.44

Weight (kDa)

6.04

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MVD-like_N PF22700 17 - 190 1.6e-65 Diphosphomevalonate decarboxylase-like N-terminal domain
GHMP_kinases_N PF00288 107 - 166 3.1e-06 GHMP kinases N terminal domain
MDD_C PF18376 204 - 405 4.4e-63 Mevalonate 5-diphosphate decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38700 AT3G54250
fragaria_vesca FvH4_7g05610 FvH4_7g05610
malus_domestica MD02G1256300.v1.1 MD07G1064100.v1.1
prunus_persica Prupe.2G078900_v2.0.a1
pyrus_communis pycom02g21690 pycom07g04970
rosa_chinensis RchiOBHm_Chr1g0332421
rosa_laevigata RLG00000029671
rosa_multiflora Rmu_sc0016943.1_g000003 Rmu_ssc0000161.1_g000007
rosa_roxburghii Rroxscaffold_4G00318190
rosa_rugosa Rorug01G0100700
rosa_samantha Rh1AG124800 Rh1BG095200 Rh1CG118900 Rh1DG129700
rosa_wichuraiana Rw1G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 4 cut(s) 502, 947, 959, 1125
AclWI GGATC 2 cut(s) 131, 539
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 261, 1030, 1157
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 4 cut(s) 74, 342, 720, 793
AfiI CCNNNNNNNGG 2 cut(s) 1202, 1250
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 3 cut(s) 680, 896, 1115
AjnI CCWGG 1 cut(s) 822
AluBI AGCT 9 cut(s) 97, 249, 275, 398, 433, 463, 507, 604, 989
AluI AGCT 9 cut(s) 97, 249, 275, 398, 433, 463, 507, 604, 989
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 2 cut(s) 131, 539
AlwNI CAGNNNCTG 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 1196
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 3, 950
ApeKI GCWGC 4 cut(s) 374, 395, 504, 989
ApoI RAATTY 3 cut(s) 261, 1030, 1157
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 3 cut(s) 950, 1144, 1205
AsuC2I CCSGG 4 cut(s) 1090, 1103, 1197, 1198
AsuHPI GGTGA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 1196
AvaII GGWCC 2 cut(s) 1144, 1205
AxyI CCTNAGG 1 cut(s) 924
BanII GRGCYC 1 cut(s) 1103
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 4 cut(s) 361, 407, 516, 976
BccI CCATC 1 cut(s) 25
BceAI ACGGC 2 cut(s) 47, 54
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 1 cut(s) 824
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 4 cut(s) 1090, 1103, 1197, 1198
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 657
BisI GCNGC 5 cut(s) 375, 396, 502, 505, 990
BlsI GCNGC 5 cut(s) 376, 397, 503, 506, 991
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 5 cut(s) 824, 1090, 1103, 1197, 1198
Bme18I GGWCC 2 cut(s) 1144, 1205
BmeT110I CYCGRG 1 cut(s) 1196
BmgT120I GGNCC 3 cut(s) 950, 1144, 1205
BmiI GGNNCC 3 cut(s) 556, 951, 1146
BmrFI CCNGG 5 cut(s) 824, 1090, 1103, 1197, 1198
BmrI ACTGGG 3 cut(s) 85, 334, 604
BmsI GCATC 4 cut(s) 129, 934, 1027, 1075
BmuI ACTGGG 3 cut(s) 85, 334, 604
BoxI GACNNNNGTC 2 cut(s) 831, 1227
BpmI CTGGAG 1 cut(s) 1116
Bpu10I CCTNAGC 1 cut(s) 391
BpuMI CCSGG 4 cut(s) 1090, 1103, 1197, 1198
BsaAI YACGTR 1 cut(s) 974
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 1089, 1162, 1196
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 1202, 1250
Bse1I ACTGG 7 cut(s) 80, 107, 329, 599, 805, 1208, 1255
Bse21I CCTNAGG 1 cut(s) 924
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 1 cut(s) 824
BseDI CCNNGG 3 cut(s) 1089, 1162, 1196
BseGI GGATG 2 cut(s) 204, 860
BseLI CCNNNNNNNGG 2 cut(s) 1202, 1250
BseMII CTCAG 3 cut(s) 56, 405, 938
BseNI ACTGG 7 cut(s) 80, 107, 329, 599, 805, 1208, 1255
BseRI GAGGAG 2 cut(s) 230, 1179
BseXI GCAGC 4 cut(s) 361, 407, 516, 976
BshFI GGCC 2 cut(s) 5, 952
BsiHKCI CYCGRG 1 cut(s) 1196
BsiSI CCGG 4 cut(s) 231, 1089, 1103, 1197
BslFI GGGAC 4 cut(s) 101, 162, 611, 1135
BslI CCNNNNNNNGG 2 cut(s) 1202, 1250
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 101, 162, 611, 1135
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 2 cut(s) 5, 952
BsoBI CYCGRG 1 cut(s) 1196
Bsp1286I GDGCHC 1 cut(s) 1103
Bsp13I TCCGGA 1 cut(s) 230
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 5 cut(s) 66, 136, 193, 451, 531
BspACI CCGC 4 cut(s) 502, 947, 959, 1125
BspANI GGCC 2 cut(s) 5, 952
BspCNI CTCAG 3 cut(s) 55, 404, 937
BspEI TCCGGA 1 cut(s) 230
BspLI GGNNCC 3 cut(s) 556, 951, 1146
BspPI GGATC 2 cut(s) 131, 539
BsrGI TGTACA 1 cut(s) 340
BsrI ACTGG 7 cut(s) 80, 107, 329, 599, 805, 1208, 1255
BssECI CCNNGG 3 cut(s) 1089, 1162, 1196
BssMI GATC 5 cut(s) 66, 136, 193, 451, 531
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 824
Bst4CI ACNGT 4 cut(s) 166, 563, 676, 800
Bst6I CTCTTC 2 cut(s) 77, 540
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 340
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 3 cut(s) 461, 499, 873
BstDEI CTNAG 4 cut(s) 42, 315, 391, 924
BstF5I GGATG 2 cut(s) 204, 860
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 5 cut(s) 69, 139, 196, 454, 534
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 5 cut(s) 66, 136, 193, 451, 531
BstMWI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
BstNI CCWGG 1 cut(s) 824
BstNSI RCATGY 2 cut(s) 341, 834
BstPAI GACNNNNGTC 2 cut(s) 831, 1227
BstSCI CCNGG 5 cut(s) 822, 1088, 1101, 1195, 1196
BstSFI CTRYAG 1 cut(s) 657
BstV1I GCAGC 4 cut(s) 361, 407, 516, 976
Bsu36I CCTNAGG 1 cut(s) 924
BsuRI GGCC 2 cut(s) 5, 952
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 204, 860
BtsIMutI CAGTG 2 cut(s) 162, 592
Cac8I GCNNGC 3 cut(s) 461, 499, 873
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 1 cut(s) 145
Cfr13I GGNCC 3 cut(s) 950, 1144, 1205
Cfr9I CCCGGG 1 cut(s) 1196
CseI GACGC 2 cut(s) 55, 139
Csp6I GTAC 4 cut(s) 73, 341, 719, 792
CviAII CATG 5 cut(s) 338, 535, 812, 831, 850
CviQI GTAC 4 cut(s) 73, 341, 719, 792
DdeI CTNAG 4 cut(s) 42, 315, 391, 924
DpnI GATC 5 cut(s) 68, 138, 195, 453, 533
DpnII GATC 5 cut(s) 66, 136, 193, 451, 531
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 77, 540
EarI CTCTTC 2 cut(s) 77, 540
Eco24I GRGCYC 1 cut(s) 1103
Eco47I GGWCC 2 cut(s) 1144, 1205
Eco81I CCTNAGG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 1196
EcoO109I RGGNCCY 1 cut(s) 1144
EcoRII CCWGG 1 cut(s) 822
EcoT38I GRGCYC 1 cut(s) 1103
FaeI CATG 5 cut(s) 341, 538, 815, 834, 853
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 4 cut(s) 101, 162, 611, 1135
FatI CATG 5 cut(s) 337, 534, 811, 830, 849
FauI CCCGC 2 cut(s) 940, 1132
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 375, 396, 502, 505, 990
FokI GGATG 2 cut(s) 211, 847
FriOI GRGCYC 1 cut(s) 1103
Fsp4HI GCNGC 5 cut(s) 375, 396, 502, 505, 990
FspBI CTAG 1 cut(s) 413
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 375, 396, 502, 505, 990
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 2 cut(s) 5, 952
HapII CCGG 4 cut(s) 231, 1089, 1103, 1197
HgaI GACGC 2 cut(s) 55, 139
HhaI GCGC 1 cut(s) 145
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 5 cut(s) 341, 538, 815, 834, 853
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 4 cut(s) 11, 240, 303, 1000
HpaII CCGG 4 cut(s) 231, 1089, 1103, 1197
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 2 cut(s) 632, 836
Hpy188I TCNGA 7 cut(s) 45, 198, 428, 1005, 1036, 1163, 1222
Hpy188III TCNNGA 4 cut(s) 231, 633, 761, 1133
Hpy8I GTNNAC 2 cut(s) 632, 836
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 313, 544, 634, 908
HpyCH4III ACNGT 4 cut(s) 166, 563, 676, 800
HpyCH4IV ACGT 2 cut(s) 441, 973
HpyCH4V TGCA 8 cut(s) 153, 337, 476, 569, 581, 779, 1040, 1188
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
HpyF3I CTNAG 4 cut(s) 42, 315, 391, 924
HpySE526I ACGT 2 cut(s) 441, 973
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 5 cut(s) 341, 538, 815, 834, 853
HspAI GCGC 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 5 cut(s) 66, 136, 193, 451, 531
Lsp1109I GCAGC 4 cut(s) 361, 407, 516, 976
LweI GCATC 4 cut(s) 129, 934, 1027, 1075
MaeI CTAG 1 cut(s) 413
MaeII ACGT 2 cut(s) 441, 973
MaeIII GTNAC 5 cut(s) 34, 127, 800, 974, 1175
MalI GATC 5 cut(s) 68, 138, 195, 453, 533
MboI GATC 5 cut(s) 66, 136, 193, 451, 531
MboII GAAGA 5 cut(s) 94, 342, 461, 557, 1226
MhlI GDGCHC 1 cut(s) 1103
MluCI AATT 5 cut(s) 261, 361, 1030, 1074, 1157
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 598
MnlI CCTC 9 cut(s) 78, 208, 227, 400, 933, 1099, 1140, 1157, 1196
MroI TCCGGA 1 cut(s) 230
MseI TTAA 2 cut(s) 254, 734
MslI CAYNNNNRTG 2 cut(s) 62, 125
MspA1I CMGCKG 2 cut(s) 398, 989
MspI CCGG 4 cut(s) 231, 1089, 1103, 1197
MspR9I CCNGG 5 cut(s) 824, 1090, 1103, 1197, 1198
Mva1269I GAATGC 1 cut(s) 669
MvaI CCWGG 1 cut(s) 824
MwoI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 776
NciI CCSGG 4 cut(s) 1090, 1103, 1197, 1198
NdeII GATC 5 cut(s) 66, 136, 193, 451, 531
NlaIII CATG 5 cut(s) 341, 538, 815, 834, 853
NlaIV GGNNCC 3 cut(s) 556, 951, 1146
NmuCI GTSAC 2 cut(s) 34, 127
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 2 cut(s) 341, 834
PciI ACATGT 1 cut(s) 830
PcsI WCGNNNNNNNCGW 1 cut(s) 1062
PctI GAATGC 1 cut(s) 669
PfeI GAWTC 3 cut(s) 240, 303, 1000
PkrI GCNGC 5 cut(s) 376, 397, 503, 506, 991
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 974
PpuMI RGGWCCY 1 cut(s) 1144
PscI ACATGT 1 cut(s) 830
PshAI GACNNNNGTC 2 cut(s) 831, 1227
Psp5II RGGWCCY 1 cut(s) 1144
Psp6I CCWGG 1 cut(s) 822
PspGI CCWGG 1 cut(s) 822
PspN4I GGNNCC 3 cut(s) 556, 951, 1146
PspPI GGNCC 3 cut(s) 950, 1144, 1205
PspPPI RGGWCCY 1 cut(s) 1144
PstNI CAGNNNCTG 1 cut(s) 398
PvuII CAGCTG 2 cut(s) 398, 989
RsaI GTAC 4 cut(s) 74, 342, 720, 793
RsaNI GTAC 4 cut(s) 73, 341, 719, 792
RseI CAYNNNNRTG 2 cut(s) 62, 125
SaqAI TTAA 2 cut(s) 254, 734
SatI GCNGC 5 cut(s) 375, 396, 502, 505, 990
Sau3AI GATC 5 cut(s) 66, 136, 193, 451, 531
Sau96I GGNCC 3 cut(s) 950, 1144, 1205
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 5 cut(s) 824, 1090, 1103, 1197, 1198
SduI GDGCHC 1 cut(s) 1103
SfaNI GCATC 4 cut(s) 129, 934, 1027, 1075
SfcI CTRYAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 1144, 1205
SmaI CCCGGG 1 cut(s) 1198
SmiMI CAYNNNNRTG 2 cut(s) 62, 125
Sse9I AATT 5 cut(s) 261, 361, 1030, 1074, 1157
SsiI CCGC 4 cut(s) 502, 947, 959, 1125
SspI AATATT 1 cut(s) 310
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 5 cut(s) 822, 1088, 1101, 1195, 1196
TaaI ACNGT 4 cut(s) 166, 563, 676, 800
TaiI ACGT 2 cut(s) 444, 976
TaqI TCGA 1 cut(s) 271
TasI AATT 5 cut(s) 261, 361, 1030, 1074, 1157
TatI WGTACW 3 cut(s) 72, 340, 791
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 3 cut(s) 240, 303, 1000
Tru1I TTAA 2 cut(s) 254, 734
Tru9I TTAA 2 cut(s) 254, 734
TscAI CASTG 2 cut(s) 169, 599
TseFI GTSAC 2 cut(s) 34, 127
TseI GCWGC 4 cut(s) 374, 395, 504, 989
Tsp45I GTSAC 2 cut(s) 34, 127
TspDTI ATGAA 4 cut(s) 342, 452, 800, 866
TspGWI ACGGA 1 cut(s) 160
TspMI CCCGGG 1 cut(s) 1196
TspRI CASTG 2 cut(s) 169, 599
VpaK11BI GGWCC 2 cut(s) 1144, 1205
XapI RAATTY 3 cut(s) 261, 1030, 1157
XceI RCATGY 2 cut(s) 341, 834
XmaI CCCGGG 1 cut(s) 1196
XspI CTAG 1 cut(s) 413
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.