pycom03g04330

15-cis-phytoene desaturase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015456
Physical Location & Seq
Forward (+)
3388315 .. 3389766
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04330.1

Sequence Viewer

Length: 1452 bp
ATGAAACTGATCACTCTTCCTCTTCCACTCCCATCCACCACCACCCTCCTCTCCCCCAAACCCCAACTCCTCCGCTTCAAATTCAAACCCCAATCATCTCTCCAGACAACTATCAACCCCCCAAAATCCCCATTTCCGCCAAATCCCAAGTCCCCTGTTATTATCATCGGCGGAGGACTCGCCGGCCTCGCCGCCGCCACCCACCTCAACTCCCAGAACACCCCTTTCCTCCTCCTCGAAGCCTCCGACGCCGTCGGCGGCCGCGTCCGAACTGACGTCGTCGACGGGTTCCTTCTCGACCGCGGCTTCCAAATCTTCATCACCGCTTACCCCGAAGCCCAGAAACTTCTCGACTACCAATCCCTCGACCTCCAGAAATTCTACTCCGGCGCCCGTATTTACTACGGCGGTAGTTTCCACACCGTTGCCGACCCCCTCCGGGATTTCTGGAACTCTGTCAAGTCCCTCGCCAACCCTATCGGATCCATTCCCGATAAATTGCTCATCGGATTGACGAGATTTCGTGTTCTGACGAAATCCGACGAAGAGATATTCTCCTCCGGCGATGAGGTCTCCACCATGGAGCTGCTGAGGGGAATCGGGTTCTCCGATTCGATCATCGCTCGATTCTTTCAGCCATTTTTCGGCGGCGTTTTCTTCGACAAAGAGCTCGAAACGACTTCCAGATTGTTCAATTTCATCTTCAAGTGTCTCGCCCTCGGCGACAACACTCTCCCATCCAACGGCATTGGCGCCATCCCACAGCAATTGGCCGCGAAATTGCCCTCTGATTCGATCGTTCTCAACTCCCGAGTCGCTTCGATTGATTTCAGCGGCGACGAGTCTTCGTCGCCAAGGGTGAGATTGGAGAGCGGAGAGGTTTTGAGAAGCGAGTTTGGAGTGATAGTGGCAGTTGAGCAACCGGAAGCGGATAAGCTTTTGGCGGGAAGGATAAATGGACTGGTCCGGACCAAACCGGCTCGAAGTACAGTTTGTTTGTATTTCACTGCAGACCGGGCCAACATTCCGGTTTCTGATCCGGTTTTGTTTCTTAACGGGTCGGGCAAGGGCATTGTGAATAACATGTTTTTCGCAACCAATGTAGCTCCATCTTACGGCCCACCCGAGAAGGCTTTGGTATCGGTATCGCTTGTCGGAGGTTTTGAGGATGTGACGGATGATGATCTGACGACTGAAGTTGTTCGTGAGCTCTCTGGCTGGTTTGGGGGATCAATGGTGGGATCTTGGAGACACTTGAGAACGTATCGAATTAAATTTGCACAACCAAACCAATGTCCGCCGCTCGATTCGAAGAGAAACCCTCGGGTCGGGTCGGGTGTGTATGTATGCGGTGACCATTGGACGACTGCCACATTTGATGGGGCGTTGGTGTCGGGAAAGAGAGCGGCAGAAGCTTTGCTTAGAGATACTGCCTTGACCAGGGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase

Protein Analysis

484

Amino Acids

52.49

Weight (kDa)

8.23

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 55 - 119 1.1e-14 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 60 - 474 2.3e-58 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015456)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09580
fragaria_vesca FvH4_3g40270
malus_domestica MD03G1054600.v1.1
prunus_persica Prupe.6G043200_v2.0.a1
pyrus_communis pycom03g04330
rosa_chinensis RchiOBHm_Chr5g0072531
rosa_laevigata RLG00000036310
rosa_multiflora Rmu_sc0002088.1_g000015 Rmu_sc0007873.1_g000006
rosa_roxburghii Rroxscaffold_1G00008610
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475400 Rh5BG495600 Rh5CG519400 Rh5DG507800
rosa_wichuraiana Rw5G044160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 2 cut(s) 389, 752
AccBSI CCGCTC 3 cut(s) 873, 1303, 1406
AccI GTMKAC 1 cut(s) 282
AccII CGCG 3 cut(s) 264, 303, 776
AccIII TCCGGA 1 cut(s) 966
AclWI GGATC 5 cut(s) 477, 490, 1031, 1237, 1249
AcoI YGGCCR 2 cut(s) 259, 771
AcsI RAATTY 3 cut(s) 80, 377, 1274
AcuI CTGAAG 1 cut(s) 1215
AcyI GRCGYC 4 cut(s) 249, 276, 390, 753
AfaI GTAC 1 cut(s) 988
AfiI CCNNNNNNNGG 6 cut(s) 439, 644, 743, 1115, 1328, 1440
AflIII ACRYGT 1 cut(s) 1083
AgsI TTSAA 4 cut(s) 79, 85, 694, 706
AjnI CCWGG 1 cut(s) 1439
AluBI AGCT 6 cut(s) 586, 670, 937, 1106, 1210, 1415
AluI AGCT 6 cut(s) 586, 670, 937, 1106, 1210, 1415
Alw21I GWGCWC 2 cut(s) 672, 1212
Alw26I GTCTC 3 cut(s) 577, 716, 1243
AlwI GGATC 5 cut(s) 477, 490, 1031, 1237, 1249
Ama87I CYCGRG 3 cut(s) 810, 1124, 1323
Aor13HI TCCGGA 1 cut(s) 966
AoxI GGCC 5 cut(s) 184, 259, 771, 1017, 1117
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 3 cut(s) 80, 377, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 1200
AspLEI GCGC 2 cut(s) 392, 755
AspS9I GGNCC 4 cut(s) 964, 969, 1017, 1118
AsuC2I CCSGG 2 cut(s) 440, 1016
AsuHPI GGTGA 3 cut(s) 313, 871, 1364
AsuII TTCGAA 1 cut(s) 1310
AvaI CYCGRG 3 cut(s) 810, 1124, 1323
AvaII GGWCC 2 cut(s) 964, 969
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 389, 752
BanII GRGCYC 2 cut(s) 672, 1212
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 672, 1212
BbvCI CCTCAGC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 573
BccI CCATC 5 cut(s) 40, 745, 764, 1117, 1373
BceAI ACGGC 4 cut(s) 236, 421, 760, 1132
BciT130I CCWGG 1 cut(s) 1441
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 2 cut(s) 440, 1016
BcoDI GTCTC 3 cut(s) 577, 716, 1243
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 2 cut(s) 393, 756
Bme1390I CCNGG 3 cut(s) 440, 1016, 1441
Bme18I GGWCC 2 cut(s) 964, 969
BmeT110I CYCGRG 3 cut(s) 810, 1124, 1323
BmgT120I GGNCC 4 cut(s) 964, 969, 1017, 1118
BmiI GGNNCC 4 cut(s) 290, 391, 484, 754
BmrFI CCNGG 3 cut(s) 440, 1016, 1441
BpiI GAAGAC 1 cut(s) 837
BplI GAGNNNNNCTC 4 cut(s) 539, 571, 1306, 1338
BpmI CTGGAG 2 cut(s) 86, 356
Bpu10I CCTNAGC 1 cut(s) 590
Bpu14I TTCGAA 1 cut(s) 1310
BpuEI CTTGAG 1 cut(s) 1276
BpuMI CCSGG 2 cut(s) 440, 1016
BsaBI GATNNNNATC 1 cut(s) 1182
BsaHI GRCGYC 4 cut(s) 249, 276, 390, 753
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 6 cut(s) 301, 579, 718, 854, 1322, 1440
BsaWI WCCGGW 4 cut(s) 922, 966, 1027, 1039
BsaXI ACNNNNNCTCC 6 cut(s) 51, 81, 194, 224, 891, 921
Bsc4I CCNNNNNNNGG 6 cut(s) 439, 644, 743, 1115, 1328, 1440
Bse118I RCCGGY 2 cut(s) 182, 976
Bse1I ACTGG 1 cut(s) 966
Bse8I GATNNNNATC 1 cut(s) 1182
BseAI TCCGGA 1 cut(s) 966
BseBI CCWGG 1 cut(s) 1441
BseDI CCNNGG 6 cut(s) 301, 579, 718, 854, 1322, 1440
BseGI GGATG 5 cut(s) 32, 737, 756, 1174, 1183
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 6 cut(s) 439, 644, 743, 1115, 1328, 1440
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 1 cut(s) 966
BseRI GAGGAG 5 cut(s) 38, 59, 221, 224, 547
BseX3I CGGCCG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 573
Bsh1236I CGCG 3 cut(s) 264, 303, 776
Bsh1285I CGRYCG 3 cut(s) 262, 301, 798
BshFI GGCC 5 cut(s) 186, 261, 773, 1019, 1119
BshNI GGYRCC 2 cut(s) 389, 752
BsiEI CGRYCG 3 cut(s) 262, 301, 798
BsiHKAI GWGCWC 2 cut(s) 672, 1212
BsiHKCI CYCGRG 3 cut(s) 810, 1124, 1323
BslFI GGGAC 2 cut(s) 136, 448
BslI CCNNNNNNNGG 6 cut(s) 439, 644, 743, 1115, 1328, 1440
BsmAI GTCTC 3 cut(s) 577, 716, 1243
BsmFI GGGAC 2 cut(s) 136, 448
BsnI GGCC 5 cut(s) 186, 261, 773, 1019, 1119
Bso31I GGTCTC 1 cut(s) 577
BsoBI CYCGRG 3 cut(s) 810, 1124, 1323
Bsp119I TTCGAA 1 cut(s) 1310
Bsp1286I GDGCHC 2 cut(s) 672, 1212
Bsp13I TCCGGA 1 cut(s) 966
Bsp143I GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
Bsp19I CCATGG 1 cut(s) 579
BspANI GGCC 5 cut(s) 186, 261, 773, 1019, 1119
BspCNI CTCAG 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 966
BspFNI CGCG 3 cut(s) 264, 303, 776
BspLI GGNNCC 4 cut(s) 290, 391, 484, 754
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 5 cut(s) 477, 490, 1031, 1237, 1249
BspT104I TTCGAA 1 cut(s) 1310
BspT107I GGYRCC 2 cut(s) 389, 752
BspTNI GGTCTC 1 cut(s) 577
BsrBI CCGCTC 3 cut(s) 873, 1303, 1406
BsrFI RCCGGY 2 cut(s) 182, 976
BsrI ACTGG 1 cut(s) 966
BssAI RCCGGY 2 cut(s) 182, 976
BssECI CCNNGG 6 cut(s) 301, 579, 718, 854, 1322, 1440
BssMI GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
BssNI GRCGYC 4 cut(s) 249, 276, 390, 753
BssT1I CCWWGG 2 cut(s) 579, 854
Bst2UI CCWGG 1 cut(s) 1441
Bst4CI ACNGT 2 cut(s) 424, 991
Bst6I CTCTTC 4 cut(s) 21, 27, 540, 1307
BstACI GRCGYC 4 cut(s) 249, 276, 390, 753
BstBI TTCGAA 1 cut(s) 1310
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 590, 1421
BstDSI CCRYGG 2 cut(s) 301, 579
BstEII GGTNACC 1 cut(s) 1352
BstENI CCTNNNNNAGG 1 cut(s) 1438
BstF5I GGATG 5 cut(s) 32, 737, 756, 1174, 1183
BstFNI CGCG 3 cut(s) 264, 303, 776
BstH2I RGCGCY 2 cut(s) 393, 756
BstHHI GCGC 2 cut(s) 392, 755
BstKTI GATC 8 cut(s) 12, 485, 618, 798, 1039, 1186, 1232, 1244
BstMAI GTCTC 3 cut(s) 577, 716, 1243
BstMBI GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
BstMCI CGRYCG 3 cut(s) 262, 301, 798
BstMWI GCNNNNNNNGC 4 cut(s) 188, 248, 1016, 1412
BstNI CCWGG 1 cut(s) 1441
BstNSI RCATGY 1 cut(s) 1087
BstPI GGTNACC 1 cut(s) 1352
BstSCI CCNGG 3 cut(s) 438, 1014, 1439
BstSFI CTRYAG 1 cut(s) 1008
BstUI CGCG 3 cut(s) 264, 303, 776
BstV1I GCAGC 1 cut(s) 573
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 2 cut(s) 482, 1241
BstYI RGATCY 2 cut(s) 482, 1241
BstZI CGGCCG 1 cut(s) 259
BsuRI GGCC 5 cut(s) 186, 261, 773, 1019, 1119
BtgI CCRYGG 2 cut(s) 301, 579
BtgZI GCGATG 2 cut(s) 579, 604
BtsCI GGATG 5 cut(s) 32, 737, 756, 1174, 1183
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 1 cut(s) 1005
Cac8I GCNNGC 1 cut(s) 184
CciNI GCGGCCGC 1 cut(s) 259
CfoI GCGC 2 cut(s) 392, 755
Cfr10I RCCGGY 2 cut(s) 182, 976
Cfr13I GGNCC 4 cut(s) 964, 969, 1017, 1118
Cfr42I CCGCGG 1 cut(s) 304
CseI GACGC 2 cut(s) 253, 257
Csp6I GTAC 1 cut(s) 987
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 2 cut(s) 580, 1084
CviQI GTAC 1 cut(s) 987
DdeI CTNAG 2 cut(s) 590, 1421
DinI GGCGCC 2 cut(s) 391, 754
DpnI GATC 8 cut(s) 11, 484, 617, 797, 1038, 1185, 1231, 1243
DpnII GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
EaeI YGGCCR 2 cut(s) 259, 771
EagI CGGCCG 1 cut(s) 259
Eam1104I CTCTTC 4 cut(s) 21, 27, 540, 1307
EarI CTCTTC 4 cut(s) 21, 27, 540, 1307
EciI GGCGGA 3 cut(s) 126, 186, 1287
Ecl136II GAGCTC 2 cut(s) 670, 1210
EclXI CGGCCG 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 579, 854
Eco24I GRGCYC 2 cut(s) 672, 1212
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 964, 969
Eco52I CGGCCG 1 cut(s) 259
Eco53kI GAGCTC 2 cut(s) 670, 1210
Eco57I CTGAAG 1 cut(s) 1215
Eco88I CYCGRG 3 cut(s) 810, 1124, 1323
Eco91I GGTNACC 1 cut(s) 1352
EcoICRI GAGCTC 2 cut(s) 670, 1210
EcoNI CCTNNNNNAGG 1 cut(s) 1438
EcoO65I GGTNACC 1 cut(s) 1352
EcoRII CCWGG 1 cut(s) 1439
EcoT14I CCWWGG 2 cut(s) 579, 854
EcoT38I GRGCYC 2 cut(s) 672, 1212
EgeI GGCGCC 2 cut(s) 391, 754
EheI GGCGCC 2 cut(s) 391, 754
ErhI CCWWGG 2 cut(s) 579, 854
FaeI CATG 2 cut(s) 583, 1087
FaiI YATR 4 cut(s) 581, 1085, 1344, 1348
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FaqI GGGAC 2 cut(s) 136, 448
FatI CATG 2 cut(s) 579, 1083
FauI CCCGC 1 cut(s) 937
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 282
FokI GGATG 5 cut(s) 19, 724, 743, 1181, 1190
FriOI GRGCYC 2 cut(s) 672, 1212
GlaI GCGC 2 cut(s) 391, 754
GsuI CTGGAG 2 cut(s) 86, 356
HaeII RGCGCY 2 cut(s) 393, 756
HaeIII GGCC 5 cut(s) 186, 261, 773, 1019, 1119
HgaI GACGC 2 cut(s) 253, 257
HhaI GCGC 2 cut(s) 392, 755
Hin1I GRCGYC 4 cut(s) 249, 276, 390, 753
Hin1II CATG 2 cut(s) 583, 1087
Hin6I GCGC 2 cut(s) 390, 753
HinP1I GCGC 2 cut(s) 390, 753
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 935, 1413
HinfI GANTC 8 cut(s) 177, 597, 611, 627, 791, 813, 842, 1307
HphI GGTGA 3 cut(s) 313, 871, 1364
Hpy166II GTNNAC 1 cut(s) 283
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 8 cut(s) 251, 257, 281, 284, 287, 545, 842, 853
HpyAV CCTTC 3 cut(s) 302, 942, 1123
HpyCH4III ACNGT 2 cut(s) 424, 991
HpyCH4IV ACGT 2 cut(s) 276, 1262
HpyCH4V TGCA 2 cut(s) 1010, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 248, 1016, 1412
HpyF3I CTNAG 2 cut(s) 590, 1421
HpySE526I ACGT 2 cut(s) 276, 1262
Hsp92I GRCGYC 4 cut(s) 249, 276, 390, 753
Hsp92II CATG 2 cut(s) 583, 1087
HspAI GCGC 2 cut(s) 390, 753
KasI GGCGCC 2 cut(s) 389, 752
Kpn2I TCCGGA 1 cut(s) 966
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 9
KspI CCGCGG 1 cut(s) 304
Kzo9I GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
LmnI GCTCC 2 cut(s) 583, 1111
Lsp1109I GCAGC 1 cut(s) 573
MaeII ACGT 2 cut(s) 276, 1262
MaeIII GTNAC 2 cut(s) 1171, 1352
MalI GATC 8 cut(s) 11, 484, 617, 797, 1038, 1185, 1231, 1243
MbiI CCGCTC 3 cut(s) 873, 1303, 1406
MboI GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
MboII GAAGA 8 cut(s) 8, 14, 307, 557, 649, 694, 837, 1324
MfeI CAATTG 1 cut(s) 767
MflI RGATCY 2 cut(s) 482, 1241
MhlI GDGCHC 2 cut(s) 672, 1212
MluCI AATT 8 cut(s) 80, 377, 497, 694, 767, 779, 1269, 1274
Mly113I GGCGCC 2 cut(s) 390, 753
MlyI GAGTC 3 cut(s) 171, 822, 851
MmeI TCCRAC 4 cut(s) 270, 564, 765, 1135
MroI TCCGGA 1 cut(s) 966
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 1200
MseI TTAA 2 cut(s) 1053, 1272
MspA1I CMGCKG 2 cut(s) 303, 834
MspR9I CCNGG 3 cut(s) 440, 1016, 1441
MunI CAATTG 1 cut(s) 767
MvaI CCWGG 1 cut(s) 1441
MvnI CGCG 3 cut(s) 264, 303, 776
MwoI GCNNNNNNNGC 4 cut(s) 188, 248, 1016, 1412
NaeI GCCGGC 1 cut(s) 184
NarI GGCGCC 2 cut(s) 390, 753
NciI CCSGG 2 cut(s) 440, 1016
NcoI CCATGG 1 cut(s) 579
NdeII GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 2 cut(s) 583, 1087
NlaIV GGNNCC 4 cut(s) 290, 391, 484, 754
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 2 cut(s) 1171, 1352
NotI GCGGCCGC 1 cut(s) 259
NspI RCATGY 1 cut(s) 1087
NspV TTCGAA 1 cut(s) 1310
PciI ACATGT 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 5 cut(s) 243, 261, 606, 720, 1122
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 5 cut(s) 597, 611, 627, 791, 1307
PflFI GACNNNGTC 2 cut(s) 251, 278
PfoI TCCNGGA 1 cut(s) 438
Ple19I CGATCG 1 cut(s) 798
PleI GAGTC 3 cut(s) 171, 821, 850
PluTI GGCGCC 2 cut(s) 393, 756
PpsI GAGTC 3 cut(s) 171, 821, 850
PscI ACATGT 1 cut(s) 1083
Psp124BI GAGCTC 2 cut(s) 672, 1212
Psp6I CCWGG 1 cut(s) 1439
PspEI GGTNACC 1 cut(s) 1352
PspGI CCWGG 1 cut(s) 1439
PspN4I GGNNCC 4 cut(s) 290, 391, 484, 754
PspPI GGNCC 4 cut(s) 964, 969, 1017, 1118
PstI CTGCAG 1 cut(s) 1012
PsuI RGATCY 2 cut(s) 482, 1241
PsyI GACNNNGTC 2 cut(s) 251, 278
PvuI CGATCG 1 cut(s) 798
RsaI GTAC 1 cut(s) 988
RsaNI GTAC 1 cut(s) 987
SacI GAGCTC 2 cut(s) 672, 1212
SacII CCGCGG 1 cut(s) 304
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 1053, 1272
Sau3AI GATC 8 cut(s) 9, 482, 615, 795, 1036, 1183, 1229, 1241
Sau96I GGNCC 4 cut(s) 964, 969, 1017, 1118
SchI GAGTC 3 cut(s) 171, 822, 851
ScrFI CCNGG 3 cut(s) 440, 1016, 1441
SduI GDGCHC 2 cut(s) 672, 1212
SfcI CTRYAG 1 cut(s) 1008
SfoI GGCGCC 2 cut(s) 391, 754
Sfr303I CCGCGG 1 cut(s) 304
SfuI TTCGAA 1 cut(s) 1310
SgrBI CCGCGG 1 cut(s) 304
SgrDI CGTCGACG 1 cut(s) 281
SinI GGWCC 2 cut(s) 964, 969
SmlI CTYRAG 1 cut(s) 1255
SmoI CTYRAG 1 cut(s) 1255
Sse9I AATT 8 cut(s) 80, 377, 497, 694, 767, 779, 1269, 1274
SspDI GGCGCC 2 cut(s) 389, 752
SstI GAGCTC 2 cut(s) 672, 1212
StyD4I CCNGG 3 cut(s) 438, 1014, 1439
StyI CCWWGG 2 cut(s) 579, 854
TaaI ACNGT 2 cut(s) 424, 991
TaiI ACGT 2 cut(s) 279, 1265
TasI AATT 8 cut(s) 80, 377, 497, 694, 767, 779, 1269, 1274
TatI WGTACW 1 cut(s) 986
TfiI GAWTC 5 cut(s) 597, 611, 627, 791, 1307
Tru1I TTAA 2 cut(s) 1053, 1272
Tru9I TTAA 2 cut(s) 1053, 1272
TscAI CASTG 1 cut(s) 1012
TseFI GTSAC 2 cut(s) 1171, 1352
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 2 cut(s) 1171, 1352
TspDTI ATGAA 3 cut(s) 17, 307, 688
TspGWI ACGGA 1 cut(s) 1190
TspRI CASTG 1 cut(s) 1012
Tth111I GACNNNGTC 2 cut(s) 251, 278
VpaK11BI GGWCC 2 cut(s) 964, 969
XagI CCTNNNNNAGG 1 cut(s) 1438
XapI RAATTY 3 cut(s) 80, 377, 1274
XceI RCATGY 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 1200
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.