RchiOBHm_Chr5g0072531

15-cis-phytoene desaturase, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015456
Physical Location & Seq
Reverse (-)
78519159 .. 78520792
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34749

Sequence Viewer

Length: 1545 bp
ATGGCTGCAGGTGTTCGTAACCCTATCCTTTCGACAATGAAGTTGATAACCTATGTCCCACATCGCACAAATGAAATTTTCCGATCAACCAGACAAACCTCCATCCTTCTTCCCCAAATGAAACTCCTCACTCTTCCTCTCTCTCATCCCTGTTCTTCAAACCCCACCCTCCTCCGCTTCAGATTCAAACCCCAATCATCTCTACAGTACCCAAATCTCACTCCCCCTAAACCCCAATCCCAAGCAACCCCAAAACCACGTGTCGTCATCATAGGCGCCGGCCTCGCCGGCCTAGCCGCAGCCACCCACCTCAACTCCCAAAACATCCCCTTCCTCCTTCTCGAAGCTTCCGATGACGTCGGCGGTCGTGTCCGAACCGACGTCGTCGACGGCTTTCTTCTCGACCGCGGATTTCAAATCCTCATTACGGCATACCCCGAAGCTCAGAAGCTTCTCGATTACCAAGCCCTCGATCTTCAAACATTCTACTCCGGCGCTCGAATCTACTATAACGGCCAGTTTCACACCGTAGCCGATCCTCTACGCAATTTCTGGGACTCCATAAAGTCCTTAGCAAACCCAATCGGATCAGTTTCTGATAAATTGCTTATCGGATTGACGAGACTCCGGGTCTTGACCAAATCCGATGAAGAGATATTTGCTTCCAGTGAAGTCTCCACGATGGAGTTGTTGAGGAAAACCGGCTTTTCGGATTCGATTATCGCCCGATTCTTTCAGCCGTTTTTCGGGGGAGTTTTCTTCGACAAGGAGCTCGAGACAACTTCTAGATTGTTCGATTTCATCTTCAAGTGTCTTGCTTTAGGCGACAACACTCTTCCTTCCAAAGGCATTGGCACCATTCCTAAACAATTGGCGGCAAAGTTGCCATCGGATTCGATCCTCTTCAATTCGAAGGTCCTTTCGATTGATTTCGAAGGGGAGGCGCCGATTGTGAGGTTGGAGAGTGGAGAGGAATTGAGTAGTGAGCTAGGAGTGATAGTGGCAGTGGAGCAACCAGAAGCGGATAAGCTTTTGGCGGGAAAGGTGTATGAACCGGTCCAAAGGAAACCGGCTCGGAGTACTGTTTGTTTATATTTCTCAACCGAACGGGCCAATATTCCGGTTCAAGACCCGGTTTTGTTTCTCAACGGTTCAGGTAAGGGGATTGTGAATAATATGTTCTTTGCAACAAATGTGGCTCCGTCTTATGGGCCAACTGGTAAGGCTTTGGTGTCAATATCCCTGGTCGGAGACTTAGAGAGTGTGAGTGATGAGGATTTGACGGTTCAAGTTGTTCGTGAGCTCTCGGATTGGTTTGAGGGAGCAATGGTAGGATCTTGGAAGCATCTAAGAACGTACCGAATCAAATTTGCACAACCAAATCAATCTCCGCCCCTTGATTCAACTAGAGATTCTCGAGTTAAGTCGGGTGTTTACGAATGCGGTGACCATGTGACCACGGCCACATTTGATGGTGCTTTGGTTTCAGGAAGAAGAGCGGTAGAAGCTTTACTCAGAGACAATGCCTTGAATAGAGTACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase

Protein Analysis

514

Amino Acids

56.57

Weight (kDa)

7.65

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 90 - 154 4.3e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 95 - 505 1.1e-56 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015456)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09580
fragaria_vesca FvH4_3g40270
malus_domestica MD03G1054600.v1.1
prunus_persica Prupe.6G043200_v2.0.a1
pyrus_communis pycom03g04330
rosa_chinensis RchiOBHm_Chr5g0072531
rosa_laevigata RLG00000036310
rosa_multiflora Rmu_sc0002088.1_g000015 Rmu_sc0007873.1_g000006
rosa_roxburghii Rroxscaffold_1G00008610
rosa_rugosa Rorug05G0418900
rosa_samantha Rh5AG475400 Rh5BG495600 Rh5CG519400 Rh5DG507800
rosa_wichuraiana Rw5G044160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 360, 384
AccB1I GGYRCC 3 cut(s) 275, 854, 943
AccBSI CCGCTC 1 cut(s) 1499
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 408
AclWI GGATC 4 cut(s) 530, 595, 892, 1342
AcoI YGGCCR 2 cut(s) 514, 1461
AcsI RAATTY 2 cut(s) 75, 1367
AcuI CTGAAG 1 cut(s) 163
AcvI CACGTG 1 cut(s) 260
AcyI GRCGYC 4 cut(s) 276, 357, 381, 944
AfaI GTAC 4 cut(s) 209, 1081, 1358, 1539
AfiI CCNNNNNNNGG 4 cut(s) 427, 746, 845, 1208
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 1054
AhdI GACNNNNNGTC 1 cut(s) 629
AjnI CCWGG 1 cut(s) 1242
AluBI AGCT 8 cut(s) 347, 443, 451, 772, 988, 1030, 1303, 1508
AluI AGCT 8 cut(s) 347, 443, 451, 772, 988, 1030, 1303, 1508
Alw21I GWGCWC 2 cut(s) 774, 1305
Alw26I GTCTC 5 cut(s) 616, 679, 770, 1245, 1512
AlwI GGATC 4 cut(s) 530, 595, 892, 1342
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 2 cut(s) 773, 1416
AoxI GGCC 6 cut(s) 280, 289, 514, 1110, 1211, 1461
ApeKI GCWGC 2 cut(s) 5, 299
ApoI RAATTY 2 cut(s) 75, 1367
AsiGI ACCGGT 1 cut(s) 1054
AspLEI GCGC 3 cut(s) 278, 497, 946
AspS9I GGNCC 4 cut(s) 916, 1057, 1110, 1211
AsuC2I CCSGG 2 cut(s) 629, 1133
AsuHPI GGTGA 1 cut(s) 1457
AsuII TTCGAA 2 cut(s) 911, 933
AvaI CYCGRG 2 cut(s) 773, 1416
AvaII GGWCC 2 cut(s) 916, 1057
BanI GGYRCC 3 cut(s) 275, 854, 943
BanII GRGCYC 2 cut(s) 774, 1305
BbrPI CACGTG 1 cut(s) 260
Bbv12I GWGCWC 2 cut(s) 774, 1305
BbvI GCAGC 1 cut(s) 311
BccI CCATC 4 cut(s) 110, 676, 895, 1466
BceAI ACGGC 5 cut(s) 406, 444, 529, 724, 1476
BciT130I CCWGG 1 cut(s) 1244
BcnI CCSGG 2 cut(s) 629, 1133
BcoDI GTCTC 5 cut(s) 616, 679, 770, 1245, 1512
BfaI CTAG 4 cut(s) 293, 786, 989, 1407
BfmI CTRYAG 2 cut(s) 6, 203
BfoI RGCGCY 3 cut(s) 279, 498, 947
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 4 cut(s) 6, 297, 300, 876
BlsI GCNGC 4 cut(s) 7, 298, 301, 877
BmcAI AGTACT 2 cut(s) 1081, 1539
Bme1390I CCNGG 3 cut(s) 629, 1133, 1244
Bme18I GGWCC 2 cut(s) 916, 1057
BmeRI GACNNNNNGTC 1 cut(s) 629
BmeT110I CYCGRG 2 cut(s) 773, 1416
BmgT120I GGNCC 4 cut(s) 916, 1057, 1110, 1211
BmiI GGNNCC 4 cut(s) 277, 856, 945, 1200
BmrFI CCNGG 3 cut(s) 629, 1133, 1244
BmsI GCATC 1 cut(s) 1354
Bpu10I CCTNAGC 1 cut(s) 571
Bpu14I TTCGAA 2 cut(s) 911, 933
BpuMI CCSGG 2 cut(s) 629, 1133
BsaAI YACGTR 1 cut(s) 260
BsaHI GRCGYC 4 cut(s) 276, 357, 381, 944
BsaJI CCNNGG 3 cut(s) 406, 1242, 1458
BsaWI WCCGGW 2 cut(s) 1054, 1120
BsaXI ACNNNNNCTCC 6 cut(s) 299, 329, 984, 1014, 1314, 1344
Bsc4I CCNNNNNNNGG 4 cut(s) 427, 746, 845, 1208
Bse118I RCCGGY 5 cut(s) 278, 287, 701, 1054, 1069
Bse1I ACTGG 3 cut(s) 517, 666, 1222
Bse3DI GCAATG 1 cut(s) 1332
BseBI CCWGG 1 cut(s) 1244
BseDI CCNNGG 3 cut(s) 406, 1242, 1458
BseGI GGATG 3 cut(s) 102, 145, 324
BseLI CCNNNNNNNGG 4 cut(s) 427, 746, 845, 1208
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 2 cut(s) 458, 1528
BseNI ACTGG 3 cut(s) 517, 666, 1222
BseRI GAGGAG 2 cut(s) 116, 161
BseXI GCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 2 cut(s) 367, 406
BshFI GGCC 6 cut(s) 282, 291, 516, 1112, 1213, 1463
BshNI GGYRCC 3 cut(s) 275, 854, 943
BshTI ACCGGT 1 cut(s) 1054
BsiEI CGRYCG 2 cut(s) 367, 406
BsiHKAI GWGCWC 2 cut(s) 774, 1305
BsiHKCI CYCGRG 2 cut(s) 773, 1416
BsiSI CCGG 9 cut(s) 279, 288, 492, 628, 702, 1055, 1070, 1121, 1133
BslFI GGGAC 2 cut(s) 41, 569
BslI CCNNNNNNNGG 4 cut(s) 427, 746, 845, 1208
BsmAI GTCTC 5 cut(s) 616, 679, 770, 1245, 1512
BsmFI GGGAC 2 cut(s) 41, 569
BsmI GAATGC 1 cut(s) 1445
BsnI GGCC 6 cut(s) 282, 291, 516, 1112, 1213, 1463
BsoBI CYCGRG 2 cut(s) 773, 1416
Bsp119I TTCGAA 2 cut(s) 911, 933
Bsp1286I GDGCHC 2 cut(s) 774, 1305
Bsp143I GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
BspANI GGCC 6 cut(s) 282, 291, 516, 1112, 1213, 1463
BspCNI CTCAG 2 cut(s) 457, 1527
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 4 cut(s) 277, 856, 945, 1200
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 4 cut(s) 530, 595, 892, 1342
BspQI GCTCTTC 1 cut(s) 1489
BspT104I TTCGAA 2 cut(s) 911, 933
BspT107I GGYRCC 3 cut(s) 275, 854, 943
BsrBI CCGCTC 1 cut(s) 1499
BsrDI GCAATG 1 cut(s) 1332
BsrFI RCCGGY 5 cut(s) 278, 287, 701, 1054, 1069
BsrI ACTGG 3 cut(s) 517, 666, 1222
BssAI RCCGGY 5 cut(s) 278, 287, 701, 1054, 1069
BssECI CCNNGG 3 cut(s) 406, 1242, 1458
BssMI GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
BssNI GRCGYC 4 cut(s) 276, 357, 381, 944
Bst2UI CCWGG 1 cut(s) 1244
Bst4CI ACNGT 5 cut(s) 207, 529, 1084, 1151, 1285
Bst6I CTCTTC 5 cut(s) 138, 645, 840, 908, 1489
BstACI GRCGYC 4 cut(s) 276, 357, 381, 944
BstBAI YACGTR 1 cut(s) 260
BstBI TTCGAA 2 cut(s) 911, 933
BstC8I GCNNGC 2 cut(s) 280, 289
BstDEI CTNAG 5 cut(s) 444, 571, 1255, 1349, 1514
BstDSI CCRYGG 2 cut(s) 406, 1458
BstEII GGTNACC 1 cut(s) 1445
BstENI CCTNNNNNAGG 1 cut(s) 843
BstF5I GGATG 3 cut(s) 102, 145, 324
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 3 cut(s) 279, 498, 947
BstHHI GCGC 3 cut(s) 278, 497, 946
BstKTI GATC 6 cut(s) 86, 475, 538, 590, 900, 1337
BstMAI GTCTC 5 cut(s) 616, 679, 770, 1245, 1512
BstMBI GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
BstMCI CGRYCG 2 cut(s) 367, 406
BstMWI GCNNNNNNNGC 4 cut(s) 284, 288, 293, 1505
BstNI CCWGG 1 cut(s) 1244
BstPI GGTNACC 1 cut(s) 1445
BstSCI CCNGG 3 cut(s) 627, 1131, 1242
BstSFI CTRYAG 2 cut(s) 6, 203
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 1334
BstYI RGATCY 1 cut(s) 1334
BsuRI GGCC 6 cut(s) 282, 291, 516, 1112, 1213, 1463
BtgI CCRYGG 2 cut(s) 406, 1458
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 102, 145, 324
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 2 cut(s) 673, 1011
Cac8I GCNNGC 2 cut(s) 280, 289
CaiI CAGNNNCTG 1 cut(s) 596
CfoI GCGC 3 cut(s) 278, 497, 946
Cfr10I RCCGGY 5 cut(s) 278, 287, 701, 1054, 1069
Cfr13I GGNCC 4 cut(s) 916, 1057, 1110, 1211
Cfr42I CCGCGG 1 cut(s) 409
Csp6I GTAC 4 cut(s) 208, 1080, 1357, 1538
CspAI ACCGGT 1 cut(s) 1054
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CviAII CATG 1 cut(s) 1451
CviQI GTAC 4 cut(s) 208, 1080, 1357, 1538
DdeI CTNAG 5 cut(s) 444, 571, 1255, 1349, 1514
DinI GGCGCC 2 cut(s) 277, 945
DpnI GATC 6 cut(s) 85, 474, 537, 589, 899, 1336
DpnII GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
DriI GACNNNNNGTC 1 cut(s) 629
EaeI YGGCCR 2 cut(s) 514, 1461
Eam1104I CTCTTC 5 cut(s) 138, 645, 840, 908, 1489
Eam1105I GACNNNNNGTC 1 cut(s) 629
EarI CTCTTC 5 cut(s) 138, 645, 840, 908, 1489
EciI GGCGGA 1 cut(s) 1380
Ecl136II GAGCTC 2 cut(s) 772, 1303
Eco24I GRGCYC 2 cut(s) 774, 1305
Eco47I GGWCC 2 cut(s) 916, 1057
Eco53kI GAGCTC 2 cut(s) 772, 1303
Eco57I CTGAAG 1 cut(s) 163
Eco72I CACGTG 1 cut(s) 260
Eco88I CYCGRG 2 cut(s) 773, 1416
Eco91I GGTNACC 1 cut(s) 1445
EcoICRI GAGCTC 2 cut(s) 772, 1303
EcoNI CCTNNNNNAGG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 916
EcoO65I GGTNACC 1 cut(s) 1445
EcoRII CCWGG 1 cut(s) 1242
EcoT38I GRGCYC 2 cut(s) 774, 1305
EgeI GGCGCC 2 cut(s) 277, 945
EheI GGCGCC 2 cut(s) 277, 945
FaeI CATG 1 cut(s) 1454
FaqI GGGAC 2 cut(s) 41, 569
FatI CATG 1 cut(s) 1450
FauI CCCGC 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 6, 297, 300, 876
FokI GGATG 3 cut(s) 89, 132, 311
FriOI GRGCYC 2 cut(s) 774, 1305
Fsp4HI GCNGC 4 cut(s) 6, 297, 300, 876
FspBI CTAG 4 cut(s) 293, 786, 989, 1407
GlaI GCGC 3 cut(s) 277, 496, 945
GluI GCNGC 4 cut(s) 6, 297, 300, 876
HaeII RGCGCY 3 cut(s) 279, 498, 947
HaeIII GGCC 6 cut(s) 282, 291, 516, 1112, 1213, 1463
HapII CCGG 9 cut(s) 279, 288, 492, 628, 702, 1055, 1070, 1121, 1133
HhaI GCGC 3 cut(s) 278, 497, 946
Hin1I GRCGYC 4 cut(s) 276, 357, 381, 944
Hin1II CATG 1 cut(s) 1454
Hin6I GCGC 3 cut(s) 276, 495, 944
HinP1I GCGC 3 cut(s) 276, 495, 944
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 4 cut(s) 345, 449, 1028, 1506
HpaII CCGG 9 cut(s) 279, 288, 492, 628, 702, 1055, 1070, 1121, 1133
HphI GGTGA 1 cut(s) 1457
Hpy166II GTNNAC 2 cut(s) 388, 1435
Hpy8I GTNNAC 2 cut(s) 388, 1435
Hpy99I CGWCG 5 cut(s) 362, 383, 386, 389, 392
HpyAV CCTTC 6 cut(s) 116, 340, 347, 849, 907, 929
HpyCH4III ACNGT 5 cut(s) 207, 529, 1084, 1151, 1285
HpyCH4IV ACGT 4 cut(s) 259, 357, 381, 1355
HpyCH4V TGCA 3 cut(s) 8, 1187, 1373
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 288, 293, 1505
HpyF3I CTNAG 5 cut(s) 444, 571, 1255, 1349, 1514
HpySE526I ACGT 4 cut(s) 259, 357, 381, 1355
Hsp92I GRCGYC 4 cut(s) 276, 357, 381, 944
Hsp92II CATG 1 cut(s) 1454
HspAI GCGC 3 cut(s) 276, 495, 944
KasI GGCGCC 2 cut(s) 275, 943
KroI GCCGGC 2 cut(s) 278, 287
KroNI GCCGGC 2 cut(s) 280, 289
KspI CCGCGG 1 cut(s) 409
Kzo9I GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
LguI GCTCTTC 1 cut(s) 1489
LmnI GCTCC 4 cut(s) 769, 1009, 1204, 1322
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 1 cut(s) 1354
MaeI CTAG 4 cut(s) 293, 786, 989, 1407
MaeII ACGT 4 cut(s) 259, 357, 381, 1355
MaeIII GTNAC 3 cut(s) 17, 1445, 1453
MalI GATC 6 cut(s) 85, 474, 537, 589, 899, 1336
MbiI CCGCTC 1 cut(s) 1499
MboI GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 1 cut(s) 1334
MhlI GDGCHC 2 cut(s) 774, 1305
MluCI AATT 7 cut(s) 75, 547, 602, 869, 907, 974, 1367
Mly113I GGCGCC 2 cut(s) 276, 944
MlyI GAGTC 2 cut(s) 551, 618
MmeI TCCRAC 2 cut(s) 939, 1228
MroNI GCCGGC 2 cut(s) 278, 287
MseI TTAA 1 cut(s) 1422
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 9 cut(s) 279, 288, 492, 628, 702, 1055, 1070, 1121, 1133
MspR9I CCNGG 3 cut(s) 629, 1133, 1244
MunI CAATTG 1 cut(s) 869
Mva1269I GAATGC 1 cut(s) 1445
MvaI CCWGG 1 cut(s) 1244
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 284, 288, 293, 1505
NaeI GCCGGC 2 cut(s) 280, 289
NarI GGCGCC 2 cut(s) 276, 944
NciI CCSGG 2 cut(s) 629, 1133
NdeII GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
NgoMIV GCCGGC 2 cut(s) 278, 287
NlaIII CATG 1 cut(s) 1454
NlaIV GGNNCC 4 cut(s) 277, 856, 945, 1200
NmuCI GTSAC 2 cut(s) 1445, 1453
NspV TTCGAA 2 cut(s) 911, 933
PaeR7I CTCGAG 2 cut(s) 773, 1416
PciSI GCTCTTC 1 cut(s) 1489
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PctI GAATGC 1 cut(s) 1445
PdiI GCCGGC 2 cut(s) 280, 289
PfeI GAWTC 8 cut(s) 183, 501, 713, 729, 893, 1362, 1400, 1412
PflFI GACNNNGTC 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 1054
PkrI GCNGC 4 cut(s) 7, 298, 301, 877
PleI GAGTC 2 cut(s) 551, 618
PluTI GGCGCC 2 cut(s) 279, 947
PmaCI CACGTG 1 cut(s) 260
PmlI CACGTG 1 cut(s) 260
PpsI GAGTC 2 cut(s) 551, 618
Ppu21I YACGTR 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 916
Psp124BI GAGCTC 2 cut(s) 774, 1305
Psp5II RGGWCCY 1 cut(s) 916
Psp6I CCWGG 1 cut(s) 1242
PspCI CACGTG 1 cut(s) 260
PspEI GGTNACC 1 cut(s) 1445
PspGI CCWGG 1 cut(s) 1242
PspN4I GGNNCC 4 cut(s) 277, 856, 945, 1200
PspPI GGNCC 4 cut(s) 916, 1057, 1110, 1211
PspPPI RGGWCCY 1 cut(s) 916
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 1 cut(s) 1334
PsyI GACNNNGTC 1 cut(s) 383
RsaI GTAC 4 cut(s) 209, 1081, 1358, 1539
RsaNI GTAC 4 cut(s) 208, 1080, 1357, 1538
SacI GAGCTC 2 cut(s) 774, 1305
SacII CCGCGG 1 cut(s) 409
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 1489
SaqAI TTAA 1 cut(s) 1422
SatI GCNGC 4 cut(s) 6, 297, 300, 876
Sau3AI GATC 6 cut(s) 83, 472, 535, 587, 897, 1334
Sau96I GGNCC 4 cut(s) 916, 1057, 1110, 1211
ScaI AGTACT 2 cut(s) 1081, 1539
SchI GAGTC 2 cut(s) 551, 618
ScrFI CCNGG 3 cut(s) 629, 1133, 1244
SduI GDGCHC 2 cut(s) 774, 1305
SfaNI GCATC 1 cut(s) 1354
SfcI CTRYAG 2 cut(s) 6, 203
SfiI GGCCNNNNNGGCC 1 cut(s) 288
SfoI GGCGCC 2 cut(s) 277, 945
Sfr274I CTCGAG 2 cut(s) 773, 1416
Sfr303I CCGCGG 1 cut(s) 409
SfuI TTCGAA 2 cut(s) 911, 933
SgrBI CCGCGG 1 cut(s) 409
SgrDI CGTCGACG 1 cut(s) 386
SinI GGWCC 2 cut(s) 916, 1057
SlaI CTCGAG 2 cut(s) 773, 1416
SmlI CTYRAG 2 cut(s) 773, 1416
SmoI CTYRAG 2 cut(s) 773, 1416
Sse9I AATT 7 cut(s) 75, 547, 602, 869, 907, 974, 1367
SspDI GGCGCC 2 cut(s) 275, 943
SspI AATATT 1 cut(s) 1117
SspMI CTAG 4 cut(s) 293, 786, 989, 1407
SstI GAGCTC 2 cut(s) 774, 1305
StyD4I CCNGG 3 cut(s) 627, 1131, 1242
TaaI ACNGT 5 cut(s) 207, 529, 1084, 1151, 1285
TaiI ACGT 4 cut(s) 262, 360, 384, 1358
TasI AATT 7 cut(s) 75, 547, 602, 869, 907, 974, 1367
TatI WGTACW 2 cut(s) 1079, 1537
TauI GCSGC 2 cut(s) 299, 878
TfiI GAWTC 8 cut(s) 183, 501, 713, 729, 893, 1362, 1400, 1412
Tru1I TTAA 1 cut(s) 1422
Tru9I TTAA 1 cut(s) 1422
TscAI CASTG 2 cut(s) 673, 1011
TseFI GTSAC 2 cut(s) 1445, 1453
TseI GCWGC 2 cut(s) 5, 299
Tsp45I GTSAC 2 cut(s) 1445, 1453
TspDTI ATGAA 6 cut(s) 53, 87, 134, 663, 790, 1065
TspGWI ACGGA 1 cut(s) 1191
TspRI CASTG 2 cut(s) 673, 1011
Tth111I GACNNNGTC 1 cut(s) 383
VpaK11BI GGWCC 2 cut(s) 916, 1057
XagI CCTNNNNNAGG 1 cut(s) 843
XapI RAATTY 2 cut(s) 75, 1367
XbaI TCTAGA 1 cut(s) 785
XhoI CTCGAG 2 cut(s) 773, 1416
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 4 cut(s) 293, 786, 989, 1407
ZraI GACGTC 2 cut(s) 358, 382
ZrmI AGTACT 2 cut(s) 1081, 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.