pycom03g08580

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0017274
Physical Location & Seq
Reverse (-)
6941231 .. 6942425
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08580.1

Sequence Viewer

Length: 651 bp
ATGCCTTTCTTAGTTTGCCAAGCAAAGAATATGAATTACTGGCCAATTAACGATGATTTGATTTGTTTTAGCACACTAGAAGGACAACCAATGGCAACACAGAATTGCAGACCTTCGTTGTTAATATGCTGTGTGCTTCTGATTGCTTTATTGGCCGGAGGTGAAAGTCTCGCACCAAGAATAAACAAGGAAAACTGTACGTACGCAGTTACAATTGAAACAACATGCACAAAGGGTGCCGAAACCTCAAACCACATAAGGCTGAGATTTGGTGACACAAACTCTAATGACATTGTGGTTCACCACCTAAACTCCAAGCACATAAGAAGGGTGGATCCGATAGCGCCTGACGTCCTTGACGATGTTCCCAGAAAACCATTTCAAGCTTGCGTGGTGGATGAGTTCCAAGTGAAGGGCCAATGTGTTGACTCGCCGGTCTGCTACCTCTACCTCAAACTGTCTGGAACTGATGATTGGAGACCTGGTTTTGCACAAGTCCATGTTTTGGAGGGGTCTCACTCCCATCTCAGCTCGAATTACTTCTACTTCCGCAGGTACTTGCCGCGCCATGTGTGGCATGGTTCGGATGTGTGTGATAAAGAGGTCACTCCATTTGGGATCAAGCACAAGAGGAAAGTGTTTGCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

217

Amino Acids

24.49

Weight (kDa)

8.0

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 62 - 198 1.2e-41 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017274)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34350
prunus_persica Prupe.6G089000_v2.0.a1
pyrus_communis pycom03g08580
rosa_laevigata RLG00000035520
rosa_multiflora Rmu_sc0011641.1_g000001
rosa_roxburghii Rroxscaffold_1G00018780
rosa_rugosa Rorug05G0345200
rosa_samantha Rh5AG406300 Rh5BG419500 Rh5CG443700 Rh5DG435000
rosa_wichuraiana Rw5G038220 Rw5G038300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 543
AccB1I GGYRCC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 565
AciI CCGC 2 cut(s) 550, 563
AclWI GGATC 3 cut(s) 329, 342, 626
AcoI YGGCCR 2 cut(s) 41, 153
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 3 cut(s) 199, 203, 557
AfiI CCNNNNNNNGG 2 cut(s) 412, 505
AgsI TTSAA 2 cut(s) 218, 383
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AluBI AGCT 2 cut(s) 386, 531
AluI AGCT 2 cut(s) 386, 531
Alw26I GTCTC 3 cut(s) 173, 472, 519
AlwI GGATC 3 cut(s) 329, 342, 626
AoxI GGCC 3 cut(s) 41, 153, 415
Asp700I GAANNNNTTC 1 cut(s) 539
AspLEI GCGC 2 cut(s) 346, 567
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 3 cut(s) 173, 284, 293
BalI TGGCCA 1 cut(s) 43
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 1 cut(s) 236
BccI CCATC 1 cut(s) 531
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 3 cut(s) 173, 472, 519
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 543
BisI GCNGC 1 cut(s) 563
BlsI GCNGC 1 cut(s) 564
Bme1390I CCNGG 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 238, 336
BmrFI CCNGG 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 2 cut(s) 472, 519
BsaXI ACNNNNNCTCC 4 cut(s) 296, 326, 469, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 505
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 44
BseBI CCWGG 1 cut(s) 483
BseGI GGATG 2 cut(s) 403, 592
BseLI CCNNNNNNNGG 2 cut(s) 412, 505
BseMII CTCAG 2 cut(s) 254, 541
BseNI ACTGG 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 565
BshFI GGCC 3 cut(s) 43, 155, 417
BshNI GGYRCC 1 cut(s) 236
BsiSI CCGG 2 cut(s) 156, 434
BsiWI CGTACG 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 412, 505
BsmAI GTCTC 3 cut(s) 173, 472, 519
BsnI GGCC 3 cut(s) 43, 155, 417
Bso31I GGTCTC 2 cut(s) 472, 519
Bsp143I GATC 2 cut(s) 334, 618
BspACI CCGC 2 cut(s) 550, 563
BspANI GGCC 3 cut(s) 43, 155, 417
BspCNI CTCAG 2 cut(s) 255, 540
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 2 cut(s) 238, 336
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 3 cut(s) 329, 342, 626
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 2 cut(s) 472, 519
BsrFI RCCGGY 1 cut(s) 433
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 433
BssMI GATC 2 cut(s) 334, 618
BssNI GRCGYC 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 197, 459
BstACI GRCGYC 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 10, 263, 527
BstF5I GGATG 2 cut(s) 403, 592
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 2 cut(s) 346, 567
BstKTI GATC 2 cut(s) 337, 621
BstMAI GTCTC 3 cut(s) 173, 472, 519
BstMBI GATC 2 cut(s) 334, 618
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 481
BstSNI TACGTA 1 cut(s) 201
BstUI CGCG 1 cut(s) 565
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 3 cut(s) 43, 155, 417
BtsCI GGATG 2 cut(s) 403, 592
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 1 cut(s) 388
CfoI GCGC 2 cut(s) 346, 567
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 415
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 3 cut(s) 198, 202, 556
CviAII CATG 4 cut(s) 225, 500, 569, 578
CviJI RGCY 6 cut(s) 43, 155, 262, 386, 417, 531
CviKI_1 RGCY 6 cut(s) 43, 155, 262, 386, 417, 531
CviQI GTAC 3 cut(s) 198, 202, 556
DdeI CTNAG 3 cut(s) 10, 263, 527
DpnI GATC 2 cut(s) 336, 620
DpnII GATC 2 cut(s) 334, 618
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 2 cut(s) 41, 153
Eco105I TACGTA 1 cut(s) 201
Eco31I GGTCTC 2 cut(s) 472, 519
EcoRII CCWGG 1 cut(s) 481
FaeI CATG 4 cut(s) 228, 503, 572, 581
FaiI YATR 8 cut(s) 32, 127, 226, 257, 323, 501, 570, 579
FatI CATG 4 cut(s) 224, 499, 568, 577
Fnu4HI GCNGC 1 cut(s) 563
FokI GGATG 2 cut(s) 410, 599
Fsp4HI GCNGC 1 cut(s) 563
FspBI CTAG 1 cut(s) 77
GlaI GCGC 2 cut(s) 345, 566
GluI GCNGC 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 3 cut(s) 43, 155, 417
HapII CCGG 2 cut(s) 156, 434
HhaI GCGC 2 cut(s) 346, 567
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 4 cut(s) 228, 503, 572, 581
Hin6I GCGC 2 cut(s) 344, 565
HinP1I GCGC 2 cut(s) 344, 565
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 1 cut(s) 428
HpaII CCGG 2 cut(s) 156, 434
HphI GGTGA 3 cut(s) 173, 284, 293
Hpy166II GTNNAC 2 cut(s) 301, 427
Hpy188I TCNGA 3 cut(s) 141, 339, 586
Hpy188III TCNNGA 1 cut(s) 462
Hpy8I GTNNAC 2 cut(s) 301, 427
HpyAV CCTTC 4 cut(s) 74, 123, 321, 406
HpyCH4III ACNGT 2 cut(s) 197, 459
HpyCH4IV ACGT 2 cut(s) 200, 351
HpyCH4V TGCA 3 cut(s) 108, 228, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 3 cut(s) 10, 263, 527
HpySE526I ACGT 2 cut(s) 200, 351
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 4 cut(s) 228, 503, 572, 581
HspAI GCGC 2 cut(s) 344, 565
Kzo9I GATC 2 cut(s) 334, 618
LpnPI CCDG 9 cut(s) 25, 169, 360, 382, 447, 447, 468, 495, 538
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 1 cut(s) 77
MaeII ACGT 2 cut(s) 200, 351
MaeIII GTNAC 3 cut(s) 208, 272, 604
MalI GATC 2 cut(s) 336, 620
MboI GATC 2 cut(s) 334, 618
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 43
MluCI AATT 5 cut(s) 34, 45, 103, 213, 535
MluNI TGGCCA 1 cut(s) 43
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 7 cut(s) 152, 256, 455, 461, 502, 595, 624
Mox20I TGGCCA 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 539
MscI TGGCCA 1 cut(s) 43
MseI TTAA 2 cut(s) 48, 122
Msp20I TGGCCA 1 cut(s) 43
MspI CCGG 2 cut(s) 156, 434
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 1 cut(s) 152
NdeII GATC 2 cut(s) 334, 618
NlaIII CATG 4 cut(s) 228, 503, 572, 581
NlaIV GGNNCC 2 cut(s) 238, 336
NmuCI GTSAC 2 cut(s) 272, 604
NspI RCATGY 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 539
Pfl23II CGTACG 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 1 cut(s) 564
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 238, 336
PspPI GGNCC 1 cut(s) 415
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 3 cut(s) 199, 203, 557
RsaNI GTAC 3 cut(s) 198, 202, 556
SaqAI TTAA 2 cut(s) 48, 122
SatI GCNGC 1 cut(s) 563
Sau3AI GATC 2 cut(s) 334, 618
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 1 cut(s) 483
SexAI ACCWGGT 1 cut(s) 481
SnaBI TACGTA 1 cut(s) 201
Sse9I AATT 5 cut(s) 34, 45, 103, 213, 535
SsiI CCGC 2 cut(s) 550, 563
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 2 cut(s) 197, 459
TaiI ACGT 2 cut(s) 203, 354
TaqI TCGA 1 cut(s) 533
TasI AATT 5 cut(s) 34, 45, 103, 213, 535
TauI GCSGC 1 cut(s) 565
Tru1I TTAA 2 cut(s) 48, 122
Tru9I TTAA 2 cut(s) 48, 122
TseFI GTSAC 2 cut(s) 272, 604
Tsp45I GTSAC 2 cut(s) 272, 604
TspDTI ATGAA 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 505
XceI RCATGY 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 1 cut(s) 77
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.